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The map label for this gene is ispB [C]

Identifier: 222525266

GI number: 222525266

Start: 2542185

End: 2543222

Strand: Direct

Name: ispB [C]

Synonym: Chy400_2006

Alternate gene names: 222525266

Gene position: 2542185-2543222 (Clockwise)

Preceding gene: 222525264

Following gene: 222525267

Centisome position: 48.25

GC content: 59.06

Gene sequence:

>1038_bases
ATGAATATGACAGCAACACCAGACGTTCGTTATCAAATTCAGGCGGAAATGCGTGCTGCATTTCCGGTGGTCGAAGGGCG
GTTGAGCCGGTTTTACGCCATGCAGGAGTACCATCTCGGCTGGCGCAATGAGGATTTGACGCCGGCGGACGCCGATCCAG
GCAAGTTGATCCGGCCACAGCTCGTATTACTGGCCTGTGCGGCTGCCGGTGGTGATCCGCAACAGGCGTTGCCGCTTGCC
GCCGGTATTCAGTTATTGCACGACTTCTCGCTCATCCACGATGACATCGAAGATGACAGTGCTATGCGGCGTGGACGGCG
CACACTGTGGCACATCTGGGGGCTGGCCCATGGCATCAATGCCGGCGATGCCATGTTTGTCATCGCTCATCTGGCTATTC
ACCGCCTGAGCGAACGCGGCTTGCCGGCAGAGCGGGTGTTATCGATTCTGCGTCGTTTCGATGAGATCATCCTCCGCATC
TGCGAAGGGCAATACCTTGATCTCAGCTTTGAGGGCGATCTGAGTATTACTCCCGCTGATTACCTGACGATGATCGGTGG
CAAAACTGCCGCTCTCATCGCCGGCGCTGCGGAACTCGGTGCCCGTGCCGCCGGCGCTGACGGAGAGACGGTGGCTGCAC
TGGCCGATTTTGGGCGAGCGGTTGGCCTGGCCTTCCAGATCGAAGACGATATTCTCGGTATCTGGGGTGCTCCCGAACAG
ACCGGTAAACCGTTCGCTGCCGATCTCTACCGCCGTAAAGTCAGTCTACCGGTTGTGCATGCGTTGACGCATAGCCCTGA
ACGCGAGCGTCTGGCTGACCTCTACCGCCAGCCCACACTCGACGAGGCTGCTGTGATGCAGGCGATGGCTATTCTCGATG
CTGCCGGATCACGTGCCTTCTGCGCTGCAATGGCAAATGAACATCATCAGGACGCCTTCAAAGCCCTGGATCGCCTGAAT
CCGGCTACCGCGGCGGCAGCCGCAGCCCGTAATCAGTTACGTTCACTGACGCAACGTCTGATTGGGCGGCAGGCGTAG

Upstream 100 bases:

>100_bases
GGAAAACATTACACGGCGATGACTCATCGGTAGTACTATTTCTCGCTTATTTATACGACAAATCGTCGTATTCGGCTGTT
AGAATTGTAAACCATTTGTA

Downstream 100 bases:

>100_bases
GTGGTCTCTCTGCCGTGTATGAGCGTGGTCATGACAGAGTCTGTGCCACTCGCTTGATGTCATCGGGCACAGAACCTAAT
CTGTTTCTCTTAAACTAATG

Product: polyprenyl synthetase

Products: NA

Alternate protein names: GGPP synthase; GGPPSase; (2E,6E)-farnesyl diphosphate synthase; Dimethylallyltranstransferase; Farnesyl diphosphate synthase; Farnesyltranstransferase; Geranylgeranyl diphosphate synthase; Geranyltranstransferase [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MNMTATPDVRYQIQAEMRAAFPVVEGRLSRFYAMQEYHLGWRNEDLTPADADPGKLIRPQLVLLACAAAGGDPQQALPLA
AGIQLLHDFSLIHDDIEDDSAMRRGRRTLWHIWGLAHGINAGDAMFVIAHLAIHRLSERGLPAERVLSILRRFDEIILRI
CEGQYLDLSFEGDLSITPADYLTMIGGKTAALIAGAAELGARAAGADGETVAALADFGRAVGLAFQIEDDILGIWGAPEQ
TGKPFAADLYRRKVSLPVVHALTHSPERERLADLYRQPTLDEAAVMQAMAILDAAGSRAFCAAMANEHHQDAFKALDRLN
PATAAAAAARNQLRSLTQRLIGRQA

Sequences:

>Translated_345_residues
MNMTATPDVRYQIQAEMRAAFPVVEGRLSRFYAMQEYHLGWRNEDLTPADADPGKLIRPQLVLLACAAAGGDPQQALPLA
AGIQLLHDFSLIHDDIEDDSAMRRGRRTLWHIWGLAHGINAGDAMFVIAHLAIHRLSERGLPAERVLSILRRFDEIILRI
CEGQYLDLSFEGDLSITPADYLTMIGGKTAALIAGAAELGARAAGADGETVAALADFGRAVGLAFQIEDDILGIWGAPEQ
TGKPFAADLYRRKVSLPVVHALTHSPERERLADLYRQPTLDEAAVMQAMAILDAAGSRAFCAAMANEHHQDAFKALDRLN
PATAAAAAARNQLRSLTQRLIGRQA
>Mature_345_residues
MNMTATPDVRYQIQAEMRAAFPVVEGRLSRFYAMQEYHLGWRNEDLTPADADPGKLIRPQLVLLACAAAGGDPQQALPLA
AGIQLLHDFSLIHDDIEDDSAMRRGRRTLWHIWGLAHGINAGDAMFVIAHLAIHRLSERGLPAERVLSILRRFDEIILRI
CEGQYLDLSFEGDLSITPADYLTMIGGKTAALIAGAAELGARAAGADGETVAALADFGRAVGLAFQIEDDILGIWGAPEQ
TGKPFAADLYRRKVSLPVVHALTHSPERERLADLYRQPTLDEAAVMQAMAILDAAGSRAFCAAMANEHHQDAFKALDRLN
PATAAAAAARNQLRSLTQRLIGRQA

Specific function: Catalyzes the trans-addition of the three molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate which is a precursor of the ether-linked lipids [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=279, Percent_Identity=30.1075268817204, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI4758430, Length=272, Percent_Identity=27.2058823529412, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI83700220, Length=272, Percent_Identity=27.2058823529412, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1789578, Length=267, Percent_Identity=32.2097378277154, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1786623, Length=260, Percent_Identity=33.4615384615385, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17505681, Length=245, Percent_Identity=29.3877551020408, Blast_Score=84, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6325188, Length=219, Percent_Identity=29.2237442922374, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24651612, Length=222, Percent_Identity=33.3333333333333, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI281365769, Length=251, Percent_Identity=28.2868525896414, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24660002, Length=251, Percent_Identity=28.2868525896414, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.1; =2.5.1.29; =2.5.1.10 [H]

Molecular weight: Translated: 37514; Mature: 37514

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMTATPDVRYQIQAEMRAAFPVVEGRLSRFYAMQEYHLGWRNEDLTPADADPGKLIRPQ
CCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
LVLLACAAAGGDPQQALPLAAGIQLLHDFSLIHDDIEDDSAMRRGRRTLWHIWGLAHGIN
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
AGDAMFVIAHLAIHRLSERGLPAERVLSILRRFDEIILRICEGQYLDLSFEGDLSITPAD
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCHH
YLTMIGGKTAALIAGAAELGARAAGADGETVAALADFGRAVGLAFQIEDDILGIWGAPEQ
HHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCEEEEECCCEEEECCCCCC
TGKPFAADLYRRKVSLPVVHALTHSPERERLADLYRQPTLDEAAVMQAMAILDAAGSRAF
CCCCHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHH
CAAMANEHHQDAFKALDRLNPATAAAAAARNQLRSLTQRLIGRQA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNMTATPDVRYQIQAEMRAAFPVVEGRLSRFYAMQEYHLGWRNEDLTPADADPGKLIRPQ
CCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
LVLLACAAAGGDPQQALPLAAGIQLLHDFSLIHDDIEDDSAMRRGRRTLWHIWGLAHGIN
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
AGDAMFVIAHLAIHRLSERGLPAERVLSILRRFDEIILRICEGQYLDLSFEGDLSITPAD
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCHH
YLTMIGGKTAALIAGAAELGARAAGADGETVAALADFGRAVGLAFQIEDDILGIWGAPEQ
HHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCEEEEECCCEEEECCCCCC
TGKPFAADLYRRKVSLPVVHALTHSPERERLADLYRQPTLDEAAVMQAMAILDAAGSRAF
CCCCHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHH
CAAMANEHHQDAFKALDRLNPATAAAAAARNQLRSLTQRLIGRQA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]