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The map label for this gene is rimO

Identifier: 222525264

GI number: 222525264

Start: 2540145

End: 2541512

Strand: Direct

Name: rimO

Synonym: Chy400_2004

Alternate gene names: 222525264

Gene position: 2540145-2541512 (Clockwise)

Preceding gene: 222525263

Following gene: 222525266

Centisome position: 48.21

GC content: 59.5

Gene sequence:

>1368_bases
ATGAAATATCACATCGTTACGCTGGGATGCCCCAAAAATGCTGTCGATAGCGAGGGAATGGATGGGCTGCTCAGCACACA
GGGTCATCAGGCAGTTGCTTCGGCTGAAGAGGCCGATGTTATTATTGTTAACACCTGTAGTTTTATCGCGGCTGCGCGTG
CCGAAACCCTTGGTGTCCTGAAAGAACTGGCCGGGCGTAAACGCCCCGGTCAACGGCTCATTGCCGCCGGTTGTATGGCG
CAGAGCCATCCACACGAGGTGGCCGGTGTGCAAGGCGTAGACGCAACGCTGGGTACGCAGCAGTGGACGCAGATCAACGC
GCTGGTCGGGCAACTGGAACGCCCGGTTATTCCGTTGACGCCAGGGCAACCGGTGGCCACCATTCCACTGACGACGACCA
CAAACGGCCAGCCGACCTCGTATGCCGATTGGCGCACAACGCAGATTCGGCGCACACACCAGACACCCTCCGCCTACCTC
AAAATCTCCGATGGCTGCAACCTGCGCTGCGCCTTCTGCACAATTCCCAGCTTCAAGGGCGATATGCGCTCGAAGCCGGT
CGGTGCGGTGCTGGCGGAAGCGCAAGAACTGGTTGCCGGAGGCGTCCGGGAGATTGTGCTGGTTGCGCAGCATCTGACCG
ACTACGGACGTGATCTTGGTTTGAAAGATGGTCTGGCCACGCTCCTGGCCGAGTTGTGTCAGGTAACGCCCCCAGAGACC
TGGATTCGCCTGATGTACGCTTACCCGCACGGTATCAGCGAGCGGCTGATTACCACGATGGCCAGCTACCCTCAAATCTG
CCACTACCTCGATATGCCGTTGCAACATGCGCATCCGGCTACGCTGCGGCGGATGCGCCGGCCACCGGACACCGACCGCA
CCCTGCGGATTATCGCTGAACTACGGGCTGCGATGCCCGATATTGCCATCCGTTCAACATTCATTGTCGGGTATCCCGGC
GAAACCACGGCTGAATTCCACGCCCTGCTGGAGTTCTTGCAAACTGCCCAACTCGACCGGGTCGGTGCCTTTCGCTACTC
CCGCGAACCAGGAACACCAGCTGCCGAATTGCCCGATCAGGTACGCCCCCAGGTGATCGAGCGGCGCTGGCACGAGCTGA
TGCGTCTGCAACAAACCATCTCCTATACGCGCAATCAGCGCTGGGTGGGGCGAACGATCAAGGTGTTGATCGAAGGGAAT
GGCACGGCAGATGATGGCAGTGCGCTGAGTATCGGGCGCTCGTTCCGTGATGCACCTGAGATTGACGGTCAGGTGTTTGT
CTGGGGCAACTATCCAGCGGGTACAATGATACCGGTACAGGTTACCCAGGCGACTGCGTACGACTTGTGGGGAGAGGCTC
TGTCGTAG

Upstream 100 bases:

>100_bases
GCCCTGGTACACGCTCGCGCTGAAACTCGATCTATAATGGAACGAGAGGATGCTGTATCCTTTTACAAGAATGTACAACA
ATATATAGAGAACGTAACCA

Downstream 100 bases:

>100_bases
ATGAACAGCAGCGAGTGGCGCGGATGTCAATCCACGCCACTCACCGTTTGGGAAAGCGATTACTTTGTTCCGTTTAACCG
CTGACTAATCTCACGCTGGA

Product: MiaB-like tRNA modifying protein YliG

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MKYHIVTLGCPKNAVDSEGMDGLLSTQGHQAVASAEEADVIIVNTCSFIAAARAETLGVLKELAGRKRPGQRLIAAGCMA
QSHPHEVAGVQGVDATLGTQQWTQINALVGQLERPVIPLTPGQPVATIPLTTTTNGQPTSYADWRTTQIRRTHQTPSAYL
KISDGCNLRCAFCTIPSFKGDMRSKPVGAVLAEAQELVAGGVREIVLVAQHLTDYGRDLGLKDGLATLLAELCQVTPPET
WIRLMYAYPHGISERLITTMASYPQICHYLDMPLQHAHPATLRRMRRPPDTDRTLRIIAELRAAMPDIAIRSTFIVGYPG
ETTAEFHALLEFLQTAQLDRVGAFRYSREPGTPAAELPDQVRPQVIERRWHELMRLQQTISYTRNQRWVGRTIKVLIEGN
GTADDGSALSIGRSFRDAPEIDGQVFVWGNYPAGTMIPVQVTQATAYDLWGEALS

Sequences:

>Translated_455_residues
MKYHIVTLGCPKNAVDSEGMDGLLSTQGHQAVASAEEADVIIVNTCSFIAAARAETLGVLKELAGRKRPGQRLIAAGCMA
QSHPHEVAGVQGVDATLGTQQWTQINALVGQLERPVIPLTPGQPVATIPLTTTTNGQPTSYADWRTTQIRRTHQTPSAYL
KISDGCNLRCAFCTIPSFKGDMRSKPVGAVLAEAQELVAGGVREIVLVAQHLTDYGRDLGLKDGLATLLAELCQVTPPET
WIRLMYAYPHGISERLITTMASYPQICHYLDMPLQHAHPATLRRMRRPPDTDRTLRIIAELRAAMPDIAIRSTFIVGYPG
ETTAEFHALLEFLQTAQLDRVGAFRYSREPGTPAAELPDQVRPQVIERRWHELMRLQQTISYTRNQRWVGRTIKVLIEGN
GTADDGSALSIGRSFRDAPEIDGQVFVWGNYPAGTMIPVQVTQATAYDLWGEALS
>Mature_455_residues
MKYHIVTLGCPKNAVDSEGMDGLLSTQGHQAVASAEEADVIIVNTCSFIAAARAETLGVLKELAGRKRPGQRLIAAGCMA
QSHPHEVAGVQGVDATLGTQQWTQINALVGQLERPVIPLTPGQPVATIPLTTTTNGQPTSYADWRTTQIRRTHQTPSAYL
KISDGCNLRCAFCTIPSFKGDMRSKPVGAVLAEAQELVAGGVREIVLVAQHLTDYGRDLGLKDGLATLLAELCQVTPPET
WIRLMYAYPHGISERLITTMASYPQICHYLDMPLQHAHPATLRRMRRPPDTDRTLRIIAELRAAMPDIAIRSTFIVGYPG
ETTAEFHALLEFLQTAQLDRVGAFRYSREPGTPAAELPDQVRPQVIERRWHELMRLQQTISYTRNQRWVGRTIKVLIEGN
GTADDGSALSIGRSFRDAPEIDGQVFVWGNYPAGTMIPVQVTQATAYDLWGEALS

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=430, Percent_Identity=26.9767441860465, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI28872782, Length=430, Percent_Identity=26.9767441860465, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI93277076, Length=458, Percent_Identity=26.4192139737991, Blast_Score=139, Evalue=6e-33,
Organism=Escherichia coli, GI1787057, Length=464, Percent_Identity=40.5172413793103, Blast_Score=308, Evalue=4e-85,
Organism=Escherichia coli, GI1786882, Length=405, Percent_Identity=30.3703703703704, Blast_Score=174, Evalue=8e-45,
Organism=Caenorhabditis elegans, GI17553146, Length=453, Percent_Identity=26.2693156732892, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71996771, Length=384, Percent_Identity=27.6041666666667, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21356207, Length=494, Percent_Identity=24.8987854251012, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI19922432, Length=458, Percent_Identity=24.8908296943231, Blast_Score=129, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_CHLAA (A9WDA3)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001635459.1
- ProteinModelPortal:   A9WDA3
- SMR:   A9WDA3
- GeneID:   5826303
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_1853
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- ProtClustDB:   CLSK973014
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR000385
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49939; Mature: 49939

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM; PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYHIVTLGCPKNAVDSEGMDGLLSTQGHQAVASAEEADVIIVNTCSFIAAARAETLGVL
CEEEEEEECCCCCCCCCCCCCCHHCCCCCHHHHCCCCCCEEEEECHHHHHHHHHHHHHHH
KELAGRKRPGQRLIAAGCMAQSHPHEVAGVQGVDATLGTQQWTQINALVGQLERPVIPLT
HHHHCCCCCCHHHHHHHHHCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECC
PGQPVATIPLTTTTNGQPTSYADWRTTQIRRTHQTPSAYLKISDGCNLRCAFCTIPSFKG
CCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCCC
DMRSKPVGAVLAEAQELVAGGVREIVLVAQHLTDYGRDLGLKDGLATLLAELCQVTPPET
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHH
WIRLMYAYPHGISERLITTMASYPQICHYLDMPLQHAHPATLRRMRRPPDTDRTLRIIAE
HHEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHCCCCCCHHHHHHHHH
LRAAMPDIAIRSTFIVGYPGETTAEFHALLEFLQTAQLDRVGAFRYSREPGTPAAELPDQ
HHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHCCCHH
VRPQVIERRWHELMRLQQTISYTRNQRWVGRTIKVLIEGNGTADDGSALSIGRSFRDAPE
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHCCEEEEEEECCCCCCCCCHHHHCCCCCCCCC
IDGQVFVWGNYPAGTMIPVQVTQATAYDLWGEALS
CCCEEEEEECCCCCCEEEEEEEHHHHHHHHHHHCC
>Mature Secondary Structure
MKYHIVTLGCPKNAVDSEGMDGLLSTQGHQAVASAEEADVIIVNTCSFIAAARAETLGVL
CEEEEEEECCCCCCCCCCCCCCHHCCCCCHHHHCCCCCCEEEEECHHHHHHHHHHHHHHH
KELAGRKRPGQRLIAAGCMAQSHPHEVAGVQGVDATLGTQQWTQINALVGQLERPVIPLT
HHHHCCCCCCHHHHHHHHHCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECC
PGQPVATIPLTTTTNGQPTSYADWRTTQIRRTHQTPSAYLKISDGCNLRCAFCTIPSFKG
CCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCCC
DMRSKPVGAVLAEAQELVAGGVREIVLVAQHLTDYGRDLGLKDGLATLLAELCQVTPPET
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHH
WIRLMYAYPHGISERLITTMASYPQICHYLDMPLQHAHPATLRRMRRPPDTDRTLRIIAE
HHEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHCCCCCCHHHHHHHHH
LRAAMPDIAIRSTFIVGYPGETTAEFHALLEFLQTAQLDRVGAFRYSREPGTPAAELPDQ
HHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHCCCHH
VRPQVIERRWHELMRLQQTISYTRNQRWVGRTIKVLIEGNGTADDGSALSIGRSFRDAPE
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHCCEEEEEEECCCCCCCCCHHHHCCCCCCCCC
IDGQVFVWGNYPAGTMIPVQVTQATAYDLWGEALS
CCCEEEEEECCCCCCEEEEEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA