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The map label for this gene is potD [H]

Identifier: 222524519

GI number: 222524519

Start: 1582007

End: 1583059

Strand: Direct

Name: potD [H]

Synonym: Chy400_1243

Alternate gene names: 222524519

Gene position: 1582007-1583059 (Clockwise)

Preceding gene: 222524517

Following gene: 222524520

Centisome position: 30.03

GC content: 51.09

Gene sequence:

>1053_bases
ATGCTACCACGTCTCATTTCTACAATCCTGCTCTGCTGCATCCTGATCGGATGTACCATACCACCATCACCGCCATCACT
GCCTTCTTCGTTGCCGGTGTTGCATATTCTGAACCGGGAAACCTCGATTGATCATGCGCTCCTGGTGCAATTTACTGCCG
AAACCGGGGTAGATATTGCGTATCATACGTACAGGAGTAGTGAAGAAGCGTACAATCTGCTAACAACGAGCAAGCAAGGC
TACGATCTGATCATCGTCAGTGATGAACTGGCCGAGCGCTTACGTCGTGAGCAAAGACTGGCTGTGCTCAATCACGAACG
GATCACACATCTGAACAACATCGATCCGGTCTTTGCCAGATCGAGCATTGATCCAGGTTATCGCTTTTGTGTGCCATACT
TGTGGCGCATCGTCGGCCTTGGTTATCGTTATTCAGCCATCGGTCGCGAGATCGAGAGTCTGGCAGCAGCGTTTGATCTG
GCTCAAATCAAGCGCATGGCCTTGATCGATGATGCACGTGTAAGTTTGGGACTGGTCCTGATTATGTTGGGGTATAGTCC
AAATACCAGTGATCCGGTCGCTATCGGTGCGGCCCGGGACTGGTTACTAACGGTGCGACCGTACATACAGTATCTTGCCG
CAAATGACGGACAGGATATGTTGCAACAAGACAGGGTTGACATTGTTGTGGAGTGGAGCGGTGATTTGTTGCAAGTGGCA
CGTACCGATGATAACCTGCGTTTTGCCATTCCCCAGGAGGGTAGTCTGTTTACGGTTGATAGCATGTGTATACCGACTGG
GTCTGCTCAGCAAGCGGCTGCCGAACAGTTTATCGATTTTATCCTGGATGCTGAAAACGGTGCAGCTCTAGCCAATACGA
CCCGCTACGGCACACCCAATCAGGCTGCGTGGCCGCTGTTAGATGCTGAGTTACAGGCGCAGATTCAGCATCAACAGACT
CTTGAGCAAAGAGGATTACTCTTTCGGCTAATTGATGTAGACCCTGCGGTACGTGAACTGTACCGTGCGGCGTGGATAGA
GGTACGCCAATGA

Upstream 100 bases:

>100_bases
TGCAACGATTTTTCATACGTATCTCCATTTGTCGGTAATAGTGGAGTGCTATTATTAAAACCGTTGAGAATGGTAAATCG
TTGCACTATCATCACACTTT

Downstream 100 bases:

>100_bases
CGTCATCAACCCCTTCATGCCGACGTCGTCTCGTTGTCGGGATTGCCGGTGCCGTTTCGTTTTCGTTATTGATCTCATTC
ATTGGGTTGGGCCTGTTCTT

Product: family 1 extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MLPRLISTILLCCILIGCTIPPSPPSLPSSLPVLHILNRETSIDHALLVQFTAETGVDIAYHTYRSSEEAYNLLTTSKQG
YDLIIVSDELAERLRREQRLAVLNHERITHLNNIDPVFARSSIDPGYRFCVPYLWRIVGLGYRYSAIGREIESLAAAFDL
AQIKRMALIDDARVSLGLVLIMLGYSPNTSDPVAIGAARDWLLTVRPYIQYLAANDGQDMLQQDRVDIVVEWSGDLLQVA
RTDDNLRFAIPQEGSLFTVDSMCIPTGSAQQAAAEQFIDFILDAENGAALANTTRYGTPNQAAWPLLDAELQAQIQHQQT
LEQRGLLFRLIDVDPAVRELYRAAWIEVRQ

Sequences:

>Translated_350_residues
MLPRLISTILLCCILIGCTIPPSPPSLPSSLPVLHILNRETSIDHALLVQFTAETGVDIAYHTYRSSEEAYNLLTTSKQG
YDLIIVSDELAERLRREQRLAVLNHERITHLNNIDPVFARSSIDPGYRFCVPYLWRIVGLGYRYSAIGREIESLAAAFDL
AQIKRMALIDDARVSLGLVLIMLGYSPNTSDPVAIGAARDWLLTVRPYIQYLAANDGQDMLQQDRVDIVVEWSGDLLQVA
RTDDNLRFAIPQEGSLFTVDSMCIPTGSAQQAAAEQFIDFILDAENGAALANTTRYGTPNQAAWPLLDAELQAQIQHQQT
LEQRGLLFRLIDVDPAVRELYRAAWIEVRQ
>Mature_350_residues
MLPRLISTILLCCILIGCTIPPSPPSLPSSLPVLHILNRETSIDHALLVQFTAETGVDIAYHTYRSSEEAYNLLTTSKQG
YDLIIVSDELAERLRREQRLAVLNHERITHLNNIDPVFARSSIDPGYRFCVPYLWRIVGLGYRYSAIGREIESLAAAFDL
AQIKRMALIDDARVSLGLVLIMLGYSPNTSDPVAIGAARDWLLTVRPYIQYLAANDGQDMLQQDRVDIVVEWSGDLLQVA
RTDDNLRFAIPQEGSLFTVDSMCIPTGSAQQAAAEQFIDFILDAENGAALANTTRYGTPNQAAWPLLDAELQAQIQHQQT
LEQRGLLFRLIDVDPAVRELYRAAWIEVRQ

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=281, Percent_Identity=29.8932384341637, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1787078, Length=361, Percent_Identity=25.4847645429363, Blast_Score=131, Evalue=7e-32,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39134; Mature: 39134

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPRLISTILLCCILIGCTIPPSPPSLPSSLPVLHILNRETSIDHALLVQFTAETGVDIA
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCEEE
YHTYRSSEEAYNLLTTSKQGYDLIIVSDELAERLRREQRLAVLNHERITHLNNIDPVFAR
EEECCCCHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCC
SSIDPGYRFCVPYLWRIVGLGYRYSAIGREIESLAAAFDLAQIKRMALIDDARVSLGLVL
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
IMLGYSPNTSDPVAIGAARDWLLTVRPYIQYLAANDGQDMLQQDRVDIVVEWSGDLLQVA
EEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEECCCEEEEE
RTDDNLRFAIPQEGSLFTVDSMCIPTGSAQQAAAEQFIDFILDAENGAALANTTRYGTPN
ECCCCEEEEECCCCCEEEECCEECCCCCHHHHHHHHHHHHHEECCCCCEEECCCCCCCCC
QAAWPLLDAELQAQIQHQQTLEQRGLLFRLIDVDPAVRELYRAAWIEVRQ
CCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLPRLISTILLCCILIGCTIPPSPPSLPSSLPVLHILNRETSIDHALLVQFTAETGVDIA
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCEEE
YHTYRSSEEAYNLLTTSKQGYDLIIVSDELAERLRREQRLAVLNHERITHLNNIDPVFAR
EEECCCCHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCC
SSIDPGYRFCVPYLWRIVGLGYRYSAIGREIESLAAAFDLAQIKRMALIDDARVSLGLVL
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
IMLGYSPNTSDPVAIGAARDWLLTVRPYIQYLAANDGQDMLQQDRVDIVVEWSGDLLQVA
EEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEECCCEEEEE
RTDDNLRFAIPQEGSLFTVDSMCIPTGSAQQAAAEQFIDFILDAENGAALANTTRYGTPN
ECCCCEEEEECCCCCEEEECCEECCCCCHHHHHHHHHHHHHEECCCCCEEECCCCCCCCC
QAAWPLLDAELQAQIQHQQTLEQRGLLFRLIDVDPAVRELYRAAWIEVRQ
CCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]