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The map label for this gene is cooH [H]

Identifier: 222524474

GI number: 222524474

Start: 1527171

End: 1528256

Strand: Direct

Name: cooH [H]

Synonym: Chy400_1198

Alternate gene names: 222524474

Gene position: 1527171-1528256 (Clockwise)

Preceding gene: 222524471

Following gene: 222524475

Centisome position: 28.98

GC content: 60.13

Gene sequence:

>1086_bases
TTGAAGTACCAACTGACACTCCATCCGGTTGCAGGAGCCTGGCATAGCGAGCGCCTGACGCTGACGATTCAGGGTGAGCG
CATTGTTGATGTTGAGTATCGCCCTTCGGTCGGTGATGGGGCGTATGCCCGGCGTCTTATGCAAGGGGGAATAACAGGCG
CATTGCAGGCTGCACGTCAGGTTTGCCCACATTGTCATGTGGCCCACACCCTGGCATTCAGCCTGGCTATCGAGCAACTC
GCCGGCGTCACCGCACCGCTCCGTGCGCAGGCGGTGCGCGTTATGCTGGCCGAACTGGAACGCGCAACCTCTCACATCAA
TACGCTGGCGGCACTGGCAACTGCGCTCGGCCTGACCGATGCGGCCCGGCTCAGCCGGTTAACGACCCGTTTGGGCTTGA
TCGCGCTGAAACTCTCTGGCAATGACGAGGCCGCGCTGATTCGGCCAGGCGGCCTGCTGGCTGATCCCAGTGAATCGGCG
CTAGCCGAAACGGCTCAGGCCATCAACGATTTATTGCCGAGGATGATAGCGGTTGCCGAGCAGAGCATTCCACGCCGCTC
ACTGCTGGCGCGCACGGTAGATATTGGCGTGTTGCAGCCAACCGCTGCCCGGCAGTTTGGTCTGGCCGGCCCACTGGCCC
GAGCAAGTGGGGTTGCCACTGATGTCCGCATTCAGGAACCTTATGCTGCTTACAACATCCTGGCCCCGGCACTGGTAACC
GAGGAGGGTGGCGATGTGCATTCCAGGGCAGTGGTTCTGTTGCTCGAAGCAATAGAGTCGTTGCGGCTGGTTGTCCGCTG
GTTGCAGAATCTCCCAATTGGCAACGTGTATGAAGAGGTTCCGTTGGTGGCCGGCGAAGCAACGGTGACGGTTGAAGCGC
CGCGTGGCTCATTGCGCTATACTGTGCGTAGCGATGGTCAGCGTTTGACCGGGGTGGAGATTGGTATTGCGCCACAGCTT
GATCGATTGCTGGCTCGCTCGCTGTTGCAGCAGGCAGCGGTTGATGATGTGATGCTGATCGCCATTTCGACCGATCCCTG
TACGGCTTGCTGGCAGTCGGCAACCTACCTGATCGGACGTAGTTAG

Upstream 100 bases:

>100_bases
GCCGGACTTCTCTGGGTACCGGTGTGTGTCCGACTTACCTACCGGCATATCACATGAGACGTGCGTGGTATAATGCGCCG
CGACAGAAAAAGGAGGTTGC

Downstream 100 bases:

>100_bases
TGTCCAGAACCCTGATGCCGCATTGCAGAGCCGTGTCTATGTAGTGTGTCACCCGGTGGAGTACCGGGTTGTTATATCGA
GGATGCATGGGTCTTATCGC

Product: NADH-ubiquinone oxidoreductase subunit 49kDa

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKYQLTLHPVAGAWHSERLTLTIQGERIVDVEYRPSVGDGAYARRLMQGGITGALQAARQVCPHCHVAHTLAFSLAIEQL
AGVTAPLRAQAVRVMLAELERATSHINTLAALATALGLTDAARLSRLTTRLGLIALKLSGNDEAALIRPGGLLADPSESA
LAETAQAINDLLPRMIAVAEQSIPRRSLLARTVDIGVLQPTAARQFGLAGPLARASGVATDVRIQEPYAAYNILAPALVT
EEGGDVHSRAVVLLLEAIESLRLVVRWLQNLPIGNVYEEVPLVAGEATVTVEAPRGSLRYTVRSDGQRLTGVEIGIAPQL
DRLLARSLLQQAAVDDVMLIAISTDPCTACWQSATYLIGRS

Sequences:

>Translated_361_residues
MKYQLTLHPVAGAWHSERLTLTIQGERIVDVEYRPSVGDGAYARRLMQGGITGALQAARQVCPHCHVAHTLAFSLAIEQL
AGVTAPLRAQAVRVMLAELERATSHINTLAALATALGLTDAARLSRLTTRLGLIALKLSGNDEAALIRPGGLLADPSESA
LAETAQAINDLLPRMIAVAEQSIPRRSLLARTVDIGVLQPTAARQFGLAGPLARASGVATDVRIQEPYAAYNILAPALVT
EEGGDVHSRAVVLLLEAIESLRLVVRWLQNLPIGNVYEEVPLVAGEATVTVEAPRGSLRYTVRSDGQRLTGVEIGIAPQL
DRLLARSLLQQAAVDDVMLIAISTDPCTACWQSATYLIGRS
>Mature_361_residues
MKYQLTLHPVAGAWHSERLTLTIQGERIVDVEYRPSVGDGAYARRLMQGGITGALQAARQVCPHCHVAHTLAFSLAIEQL
AGVTAPLRAQAVRVMLAELERATSHINTLAALATALGLTDAARLSRLTTRLGLIALKLSGNDEAALIRPGGLLADPSESA
LAETAQAINDLLPRMIAVAEQSIPRRSLLARTVDIGVLQPTAARQFGLAGPLARASGVATDVRIQEPYAAYNILAPALVT
EEGGDVHSRAVVLLLEAIESLRLVVRWLQNLPIGNVYEEVPLVAGEATVTVEAPRGSLRYTVRSDGQRLTGVEIGIAPQL
DRLLARSLLQQAAVDDVMLIAISTDPCTACWQSATYLIGRS

Specific function: The carbon monoxide dehydrogenase (CODH) oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (probably CooH) [H]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli formate hydrogenlyase hydrogenase isozyme 3 and to bovine mitochondrial NADH-ubiquinone oxidoreductase [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=266, Percent_Identity=28.1954887218045, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI260898743, Length=266, Percent_Identity=28.1954887218045, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1789076, Length=349, Percent_Identity=28.3667621776504, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1788832, Length=352, Percent_Identity=27.5568181818182, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI145693162, Length=355, Percent_Identity=24.5070422535211, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17555284, Length=263, Percent_Identity=28.5171102661597, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17568379, Length=263, Percent_Identity=27.3764258555133, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI221459469, Length=212, Percent_Identity=31.6037735849057, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24638644, Length=207, Percent_Identity=29.951690821256, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001501 [H]

Pfam domain/function: PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 38479; Mature: 38479

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYQLTLHPVAGAWHSERLTLTIQGERIVDVEYRPSVGDGAYARRLMQGGITGALQAARQ
CCEEEEEECCCCCCCCCEEEEEEECCEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHH
VCPHCHVAHTLAFSLAIEQLAGVTAPLRAQAVRVMLAELERATSHINTLAALATALGLTD
HCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
AARLSRLTTRLGLIALKLSGNDEAALIRPGGLLADPSESALAETAQAINDLLPRMIAVAE
HHHHHHHHHHHCEEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QSIPRRSLLARTVDIGVLQPTAARQFGLAGPLARASGVATDVRIQEPYAAYNILAPALVT
HCCCHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHCCCEEEEEECCCHHHHHHHHHHHEE
EEGGDVHSRAVVLLLEAIESLRLVVRWLQNLPIGNVYEEVPLVAGEATVTVEAPRGSLRY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCEEECCEEEEEECCCCCEEE
TVRSDGQRLTGVEIGIAPQLDRLLARSLLQQAAVDDVMLIAISTDPCTACWQSATYLIGR
EEECCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHCCCEEEEC
S
C
>Mature Secondary Structure
MKYQLTLHPVAGAWHSERLTLTIQGERIVDVEYRPSVGDGAYARRLMQGGITGALQAARQ
CCEEEEEECCCCCCCCCEEEEEEECCEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHH
VCPHCHVAHTLAFSLAIEQLAGVTAPLRAQAVRVMLAELERATSHINTLAALATALGLTD
HCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
AARLSRLTTRLGLIALKLSGNDEAALIRPGGLLADPSESALAETAQAINDLLPRMIAVAE
HHHHHHHHHHHCEEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QSIPRRSLLARTVDIGVLQPTAARQFGLAGPLARASGVATDVRIQEPYAAYNILAPALVT
HCCCHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHCCCEEEEEECCCHHHHHHHHHHHEE
EEGGDVHSRAVVLLLEAIESLRLVVRWLQNLPIGNVYEEVPLVAGEATVTVEAPRGSLRY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCEEECCEEEEEECCCCCEEE
TVRSDGQRLTGVEIGIAPQLDRLLARSLLQQAAVDDVMLIAISTDPCTACWQSATYLIGR
EEECCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHCCCEEEEC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8626276; 1644755 [H]