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The map label for this gene is nfo [H]

Identifier: 222524430

GI number: 222524430

Start: 1463552

End: 1464409

Strand: Direct

Name: nfo [H]

Synonym: Chy400_1154

Alternate gene names: 222524430

Gene position: 1463552-1464409 (Clockwise)

Preceding gene: 222524428

Following gene: 222524431

Centisome position: 27.78

GC content: 56.29

Gene sequence:

>858_bases
ATGCCGCGATTTGGTGCACACATGTCGATTTCGGGCGGCGTCTCAAAATCGTTTGCCCGTGGTGAATCGGTTGGCCTGGA
CTCGATGCAGATTTTCGCCAAAAACGAACGGCAGTGGACAGCGAAACCCATCTCGCCGGAAGAGGCGACAGCATTTCGGA
CAGAGCAACAACGTACCGGTATTCATCCGGTCGTCGTCCACGACTCCTACCTCATCAATCTGGCAGCGCCAGCCGATGAA
CTGCGCGAGAAGTCGATTGCAGCGTTCGCCGACGAGCTGGAACGTTGTGCACAACTCGATATTCCGTATCTGGTGACCCA
TCCCGGTGCCCATACCGGCATTGGTGAAGAGGCTGGTCTGGCGCGGGTTGCTGATGCGATTTGCCGGCTGTTGGCAGAGG
GTGTCGGCGGTAATACGATGATCTTGCTTGAGACGACGGCAGGGCAGGGGACAGCGCTGGGTTACCGCTTTGAGCATTTG
GCCCGCCTCTTCGAATTGATCCCGTACCACGAGCGGTTGGGGATTTGTGTTGACACCTGTCATATCTTTGCGGCCGGCTA
CGACATTCGTGATCCCGAAGGGTATCAGACCACATTTGCCGAGCTTGACCGTCTGGTTGGCCTGACACGGGTCAAATGCT
TTCATCTAAACGACTCCCAGAAGGATTTGGGTAGTCGGGTGGATCGGCATGCGCACATTGGGCAGGGTTGTATCGGTGTT
GAGGCGTTCCGTATGCTGGTGAATGACCCACGCTTTGCCGATTTGCCGATGATTATCGAAACCCCAAAAGGCGAGGATAT
GGCTGAGGATCGCATGAATCTGGCCCTGCTGCGTTCGCTCGTACAGGGTGCCGAATAG

Upstream 100 bases:

>100_bases
TCCCATCCTCACCGACTACCCTTCTATCCGGCTTCCGTGAATGCCGTGATATAATTGCAGTATCCGGTACTCGCCGGCAC
AAAGTGATTAAGGTGACTGC

Downstream 100 bases:

>100_bases
AGGTATGGATCCGCATCTACAACCGGGGAGCAAACTGTTATGTGGCGTAAGTTGCTGTTGGTTGCCTTATTACTCGGGAT
TGGATACGTGATCTGGCGCC

Product: apurinic endonuclease Apn1

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MPRFGAHMSISGGVSKSFARGESVGLDSMQIFAKNERQWTAKPISPEEATAFRTEQQRTGIHPVVVHDSYLINLAAPADE
LREKSIAAFADELERCAQLDIPYLVTHPGAHTGIGEEAGLARVADAICRLLAEGVGGNTMILLETTAGQGTALGYRFEHL
ARLFELIPYHERLGICVDTCHIFAAGYDIRDPEGYQTTFAELDRLVGLTRVKCFHLNDSQKDLGSRVDRHAHIGQGCIGV
EAFRMLVNDPRFADLPMIIETPKGEDMAEDRMNLALLRSLVQGAE

Sequences:

>Translated_285_residues
MPRFGAHMSISGGVSKSFARGESVGLDSMQIFAKNERQWTAKPISPEEATAFRTEQQRTGIHPVVVHDSYLINLAAPADE
LREKSIAAFADELERCAQLDIPYLVTHPGAHTGIGEEAGLARVADAICRLLAEGVGGNTMILLETTAGQGTALGYRFEHL
ARLFELIPYHERLGICVDTCHIFAAGYDIRDPEGYQTTFAELDRLVGLTRVKCFHLNDSQKDLGSRVDRHAHIGQGCIGV
EAFRMLVNDPRFADLPMIIETPKGEDMAEDRMNLALLRSLVQGAE
>Mature_284_residues
PRFGAHMSISGGVSKSFARGESVGLDSMQIFAKNERQWTAKPISPEEATAFRTEQQRTGIHPVVVHDSYLINLAAPADEL
REKSIAAFADELERCAQLDIPYLVTHPGAHTGIGEEAGLARVADAICRLLAEGVGGNTMILLETTAGQGTALGYRFEHLA
RLFELIPYHERLGICVDTCHIFAAGYDIRDPEGYQTTFAELDRLVGLTRVKCFHLNDSQKDLGSRVDRHAHIGQGCIGVE
AFRMLVNDPRFADLPMIIETPKGEDMAEDRMNLALLRSLVQGAE

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788483, Length=273, Percent_Identity=42.4908424908425, Blast_Score=224, Evalue=4e-60,
Organism=Caenorhabditis elegans, GI17531193, Length=265, Percent_Identity=38.4905660377359, Blast_Score=200, Evalue=7e-52,
Organism=Saccharomyces cerevisiae, GI6322735, Length=267, Percent_Identity=34.8314606741573, Blast_Score=181, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 31302; Mature: 31171

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00729 AP_NUCLEASE_F2_1 ; PS00730 AP_NUCLEASE_F2_2 ; PS00731 AP_NUCLEASE_F2_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRFGAHMSISGGVSKSFARGESVGLDSMQIFAKNERQWTAKPISPEEATAFRTEQQRTG
CCCCCCCEEECCCCCHHHHCCCCCCCCHHEEEECCCCCEECCCCCCCHHHHHHHHHHHCC
IHPVVVHDSYLINLAAPADELREKSIAAFADELERCAQLDIPYLVTHPGAHTGIGEEAGL
CCEEEEECCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHH
ARVADAICRLLAEGVGGNTMILLETTAGQGTALGYRFEHLARLFELIPYHERLGICVDTC
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHCHHHHHH
HIFAAGYDIRDPEGYQTTFAELDRLVGLTRVKCFHLNDSQKDLGSRVDRHAHIGQGCIGV
HHHEECCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHCCCCHHHH
EAFRMLVNDPRFADLPMIIETPKGEDMAEDRMNLALLRSLVQGAE
HHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PRFGAHMSISGGVSKSFARGESVGLDSMQIFAKNERQWTAKPISPEEATAFRTEQQRTG
CCCCCCEEECCCCCHHHHCCCCCCCCHHEEEECCCCCEECCCCCCCHHHHHHHHHHHCC
IHPVVVHDSYLINLAAPADELREKSIAAFADELERCAQLDIPYLVTHPGAHTGIGEEAGL
CCEEEEECCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHH
ARVADAICRLLAEGVGGNTMILLETTAGQGTALGYRFEHLARLFELIPYHERLGICVDTC
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHCHHHHHH
HIFAAGYDIRDPEGYQTTFAELDRLVGLTRVKCFHLNDSQKDLGSRVDRHAHIGQGCIGV
HHHEECCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHCCCCHHHH
EAFRMLVNDPRFADLPMIIETPKGEDMAEDRMNLALLRSLVQGAE
HHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA