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The map label for this gene is menC [H]

Identifier: 222524213

GI number: 222524213

Start: 1178878

End: 1179987

Strand: Direct

Name: menC [H]

Synonym: Chy400_0934

Alternate gene names: 222524213

Gene position: 1178878-1179987 (Clockwise)

Preceding gene: 222524212

Following gene: 222524214

Centisome position: 22.37

GC content: 61.71

Gene sequence:

>1110_bases
ATGCGGATTGAGGCCATCGAATTGCGACGGATCCGGTTGCCATACCGGGCACCCTTCGAGACGAGCGGCTGGCGGGAAGA
GGCCAATCATTCGGTGATTGTGACCCTGCATAGCGAGGGGGTAGTGGGCTGGGGCGAAGCTCCGGTTGGCGTGGGGCCGT
GGTACAACGAAGAGACCCAATCTACCGCCTGGGTGATGATGCGCGAGATTCTGGCCCCGATGCTGCTGGGGCGTGATCTG
AGCCGGCCAGAGGAGGTGGATGCGATCTTTACCCGTGTGCGCGGCAACCGCATGGCACGGGCCGGTCTTGAGTTCGCCGC
CTGGGATCTGTTCGGGCGCGCACAGGGCACGAGCCTGAGCACAATGCTCGGCGGCACCCGGCAACGGGTGGAAGTTGGTG
TCAGTGTTGGTGTCCAGGAGTCGATAGAGGCGTTGCTTGATGTGGTCGCCGGTTATGTCAATGCCGGTTACCAGCGGGTG
AAACTCAAGATCAAACCGGGCTGGGATGTCGAGCCAACACGGGCTGTACGCGAGCGCTGGCCCGATCTGCGCTTGCAGGT
TGATGCCAACAGCATCTACACCCTCGATCAGGCCGACCAGTTGGCCCGGCTCGACGAGTTTGATCTGTTGTTGATCGAGC
AGCCCCTGGCGCATGACGACATCATCGATCACGCCAAACTTCAGCGGCTGCTCCGCACGCCGATTTGTCTCGATGAGAGC
ATTGTCTCGCCAGATCATGCCCGCTGGGCAATTGAGTTGGGTGCCTGCGGCGTAATCAATATCAAACCGTCGCGGGTTGG
TGGTTTTACGGCAGCCCGGCGAATTCACGATCTGGCACAGGCCGCCGGCATCCCGGTCTGGTGCGGCGGCATGCTCGAAA
CCGGCATCGGGCGGGCTGCCAACGTCGGGCTGGCCAGTCTGCCCAACTTTAGCCTGCCCGGCGACATCAGCGCGAATGAT
CGCTACTTCCACCAGGACATTGTGACCAACCCGTTTACGCTCAACCCCGACAGTACACTCAGCGTCCCAACCGGGCCAGG
GAGTGGGGCTGAGGTCGATCTGGAACGTCTTGCGGCAGTCACGACCGATTATGTCATGCTGCGTGCGTAG

Upstream 100 bases:

>100_bases
TTATTCCGGCGGCGTTGGCCTACGGCAATCGCGGGGCGGGTGGTGTGATCCCACCAGGAGCAACTCTGATCTTTGAAGTG
GAGCTGTTGGAGGTGCGTTG

Downstream 100 bases:

>100_bases
CAGCATGTGGAATGCATGATGGAACAGCGCCCAATTCAATGGCTGGTGAGCGGCGTGGTGGCGCTCGTGATGATTGGTCT
GCTCGTGTTCGTCAACAGCG

Product: Mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: OSB synthase; OSBS; 4-(2'-carboxyphenyl)-4-oxybutyric acid synthase; o-succinylbenzoic acid synthase [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MRIEAIELRRIRLPYRAPFETSGWREEANHSVIVTLHSEGVVGWGEAPVGVGPWYNEETQSTAWVMMREILAPMLLGRDL
SRPEEVDAIFTRVRGNRMARAGLEFAAWDLFGRAQGTSLSTMLGGTRQRVEVGVSVGVQESIEALLDVVAGYVNAGYQRV
KLKIKPGWDVEPTRAVRERWPDLRLQVDANSIYTLDQADQLARLDEFDLLLIEQPLAHDDIIDHAKLQRLLRTPICLDES
IVSPDHARWAIELGACGVINIKPSRVGGFTAARRIHDLAQAAGIPVWCGGMLETGIGRAANVGLASLPNFSLPGDISAND
RYFHQDIVTNPFTLNPDSTLSVPTGPGSGAEVDLERLAAVTTDYVMLRA

Sequences:

>Translated_369_residues
MRIEAIELRRIRLPYRAPFETSGWREEANHSVIVTLHSEGVVGWGEAPVGVGPWYNEETQSTAWVMMREILAPMLLGRDL
SRPEEVDAIFTRVRGNRMARAGLEFAAWDLFGRAQGTSLSTMLGGTRQRVEVGVSVGVQESIEALLDVVAGYVNAGYQRV
KLKIKPGWDVEPTRAVRERWPDLRLQVDANSIYTLDQADQLARLDEFDLLLIEQPLAHDDIIDHAKLQRLLRTPICLDES
IVSPDHARWAIELGACGVINIKPSRVGGFTAARRIHDLAQAAGIPVWCGGMLETGIGRAANVGLASLPNFSLPGDISAND
RYFHQDIVTNPFTLNPDSTLSVPTGPGSGAEVDLERLAAVTTDYVMLRA
>Mature_369_residues
MRIEAIELRRIRLPYRAPFETSGWREEANHSVIVTLHSEGVVGWGEAPVGVGPWYNEETQSTAWVMMREILAPMLLGRDL
SRPEEVDAIFTRVRGNRMARAGLEFAAWDLFGRAQGTSLSTMLGGTRQRVEVGVSVGVQESIEALLDVVAGYVNAGYQRV
KLKIKPGWDVEPTRAVRERWPDLRLQVDANSIYTLDQADQLARLDEFDLLLIEQPLAHDDIIDHAKLQRLLRTPICLDES
IVSPDHARWAIELGACGVINIKPSRVGGFTAARRIHDLAQAAGIPVWCGGMLETGIGRAANVGLASLPNFSLPGDISAND
RYFHQDIVTNPFTLNPDSTLSVPTGPGSGAEVDLERLAAVTTDYVMLRA

Specific function: Converts SHCHC to OSB [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=305, Percent_Identity=29.8360655737705, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354
- InterPro:   IPR010197 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.113 [H]

Molecular weight: Translated: 40623; Mature: 40623

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIEAIELRRIRLPYRAPFETSGWREEANHSVIVTLHSEGVVGWGEAPVGVGPWYNEETQ
CCCCCEEHEEEECCCCCCCCCCCCHHHCCCEEEEEEECCCEEECCCCCCCCCCCCCCCCC
STAWVMMREILAPMLLGRDLSRPEEVDAIFTRVRGNRMARAGLEFAAWDLFGRAQGTSLS
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHCCCCCCHH
TMLGGTRQRVEVGVSVGVQESIEALLDVVAGYVNAGYQRVKLKIKPGWDVEPTRAVRERW
HHHCCCHHEEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHCC
PDLRLQVDANSIYTLDQADQLARLDEFDLLLIEQPLAHDDIIDHAKLQRLLRTPICLDES
CCEEEEECCCCEEEECCHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCC
IVSPDHARWAIELGACGVINIKPSRVGGFTAARRIHDLAQAAGIPVWCGGMLETGIGRAA
CCCCCCCEEEEEECCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEECHHHHHCCCCHH
NVGLASLPNFSLPGDISANDRYFHQDIVTNPFTLNPDSTLSVPTGPGSGAEVDLERLAAV
CCCHHCCCCCCCCCCCCCCCCEEHHHHCCCCEEECCCCEEECCCCCCCCCCHHHHHHHHH
TTDYVMLRA
HHCEEEEEC
>Mature Secondary Structure
MRIEAIELRRIRLPYRAPFETSGWREEANHSVIVTLHSEGVVGWGEAPVGVGPWYNEETQ
CCCCCEEHEEEECCCCCCCCCCCCHHHCCCEEEEEEECCCEEECCCCCCCCCCCCCCCCC
STAWVMMREILAPMLLGRDLSRPEEVDAIFTRVRGNRMARAGLEFAAWDLFGRAQGTSLS
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHCCCCCCHH
TMLGGTRQRVEVGVSVGVQESIEALLDVVAGYVNAGYQRVKLKIKPGWDVEPTRAVRERW
HHHCCCHHEEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHCC
PDLRLQVDANSIYTLDQADQLARLDEFDLLLIEQPLAHDDIIDHAKLQRLLRTPICLDES
CCEEEEECCCCEEEECCHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCC
IVSPDHARWAIELGACGVINIKPSRVGGFTAARRIHDLAQAAGIPVWCGGMLETGIGRAA
CCCCCCCEEEEEECCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEECHHHHHCCCCHH
NVGLASLPNFSLPGDISANDRYFHQDIVTNPFTLNPDSTLSVPTGPGSGAEVDLERLAAV
CCCHHCCCCCCCCCCCCCCCCEEHHHHCCCCEEECCCCEEECCCCCCCCCCHHHHHHHHH
TTDYVMLRA
HHCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 10194342 [H]