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The map label for this gene is dapA

Identifier: 222151297

GI number: 222151297

Start: 1159019

End: 1159909

Strand: Direct

Name: dapA

Synonym: MCCL_1050

Alternate gene names: 222151297

Gene position: 1159019-1159909 (Clockwise)

Preceding gene: 222151296

Following gene: 222151298

Centisome position: 55.13

GC content: 38.5

Gene sequence:

>891_bases
ATGAACATACACTTTAAAGGAACAGGTGTTGCAGTAACCACACCATTCAACGGACAGACGATAGATTACAACTTATTTGA
AGATCATTTAAATTTTCTTATCAACAACAATGTTGAAGCATTGATAATTAATGGAACAACTGGTGAAGGTTCTACATTAA
CAGAAGATGAAAAACTCAAAACGATTGAAATAGCGGTGCGCGTTGCACATGGCAGAGTTCCTGTAATTGCTGGCACAGGA
ACCAACAATACTCAGGCAACAATTGAACATTCATTAAAGGCCAAAGCTTTAGGCGTTGATAGCATCATGCTAATTACCCC
TTACTATAATAAAACAAATCAGCGTGGTCTTCTCGCACATTTCACTACAATTGCAGATGCTGTAGAACTACCGGTATTAC
TCTATAATGTACCTGCCAGAACAAATATGACGATAGAGCAGGAAACTGTTGCAGCCCTGGCAGAGCATCCTTATATATAC
GGTATAAAAGATGCGACAGGTGATATAAACTATATGAAGACACTAAAATCTGTAGTCCCCGATGACTTTGCATTATATAG
TGGCAATGATGATGCTGTCCTGCCATTTTATGAAGCGGGTGGTGATGGCGTGATTTCAGTTATTGCAAATGCGATACCAG
CAGAGTTCAGTGATATTTACCGAACATATCAGGTTAATCGCCATGAAGCTGAGCGTCAGTTCAACAACCTACTCCCTCTC
ATAAATGCATTATCTGTAGACGTAAATCCTATACCAATCAAAGCATTGGTCGCTTATATCGGTTATGCGAATGGTGAATT
ACGATTACCTTTAGTTCCAATGCTAGAACAAGATACAAAACAGCTGATTGAATTATATAATCGTATTGCTAAAGGAAGTG
ATCTTTCATGA

Upstream 100 bases:

>100_bases
CTGCCGATAACCTTCTAAAAGGTGCAGCACTAAATGCGGTTCAAATTTTAGAACAGATCCATACGCACCAATTAAATCGA
TGATTCTAAGGAGCGATATA

Downstream 100 bases:

>100_bases
AGATTATTTTAAATGGTTACGGAGCTATGAATGAACGTGTGGCACATCTAGCTGAGAAGAGAGGACATGAAATAGCAGGT
ATAATATTAAGTAAAAAAAA

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MNIHFKGTGVAVTTPFNGQTIDYNLFEDHLNFLINNNVEALIINGTTGEGSTLTEDEKLKTIEIAVRVAHGRVPVIAGTG
TNNTQATIEHSLKAKALGVDSIMLITPYYNKTNQRGLLAHFTTIADAVELPVLLYNVPARTNMTIEQETVAALAEHPYIY
GIKDATGDINYMKTLKSVVPDDFALYSGNDDAVLPFYEAGGDGVISVIANAIPAEFSDIYRTYQVNRHEAERQFNNLLPL
INALSVDVNPIPIKALVAYIGYANGELRLPLVPMLEQDTKQLIELYNRIAKGSDLS

Sequences:

>Translated_296_residues
MNIHFKGTGVAVTTPFNGQTIDYNLFEDHLNFLINNNVEALIINGTTGEGSTLTEDEKLKTIEIAVRVAHGRVPVIAGTG
TNNTQATIEHSLKAKALGVDSIMLITPYYNKTNQRGLLAHFTTIADAVELPVLLYNVPARTNMTIEQETVAALAEHPYIY
GIKDATGDINYMKTLKSVVPDDFALYSGNDDAVLPFYEAGGDGVISVIANAIPAEFSDIYRTYQVNRHEAERQFNNLLPL
INALSVDVNPIPIKALVAYIGYANGELRLPLVPMLEQDTKQLIELYNRIAKGSDLS
>Mature_296_residues
MNIHFKGTGVAVTTPFNGQTIDYNLFEDHLNFLINNNVEALIINGTTGEGSTLTEDEKLKTIEIAVRVAHGRVPVIAGTG
TNNTQATIEHSLKAKALGVDSIMLITPYYNKTNQRGLLAHFTTIADAVELPVLLYNVPARTNMTIEQETVAALAEHPYIY
GIKDATGDINYMKTLKSVVPDDFALYSGNDDAVLPFYEAGGDGVISVIANAIPAEFSDIYRTYQVNRHEAERQFNNLLPL
INALSVDVNPIPIKALVAYIGYANGELRLPLVPMLEQDTKQLIELYNRIAKGSDLS

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Homo sapiens, GI31543060, Length=269, Percent_Identity=29.7397769516729, Blast_Score=133, Evalue=2e-31,
Organism=Escherichia coli, GI1788823, Length=279, Percent_Identity=38.7096774193548, Blast_Score=184, Evalue=5e-48,
Organism=Escherichia coli, GI87082415, Length=264, Percent_Identity=34.469696969697, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1786463, Length=197, Percent_Identity=35.0253807106599, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1789620, Length=223, Percent_Identity=26.457399103139, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): DAPA_MACCJ (B9EBZ6)

Other databases:

- EMBL:   AP009484
- RefSeq:   YP_002560453.1
- ProteinModelPortal:   B9EBZ6
- GeneID:   7390197
- GenomeReviews:   AP009484_GR
- KEGG:   mcl:MCCL_1050
- ProtClustDB:   PRK03170
- GO:   GO:0005737
- HAMAP:   MF_00418
- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146
- TIGRFAMs:   TIGR00674

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.52

Molecular weight: Translated: 32422; Mature: 32422

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 163-163 BINDING 108-108

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIHFKGTGVAVTTPFNGQTIDYNLFEDHLNFLINNNVEALIINGTTGEGSTLTEDEKLK
CEEEEECCCEEEECCCCCCEEEEHHHHHHHHEEECCCCEEEEEECCCCCCCCCCCCCCEE
TIEIAVRVAHGRVPVIAGTGTNNTQATIEHSLKAKALGVDSIMLITPYYNKTNQRGLLAH
EEEEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHH
FTTIADAVELPVLLYNVPARTNMTIEQETVAALAEHPYIYGIKDATGDINYMKTLKSVVP
HHHHHHHHHCCEEEEECCCCCCCEEHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCC
DDFALYSGNDDAVLPFYEAGGDGVISVIANAIPAEFSDIYRTYQVNRHEAERQFNNLLPL
CCEEEEECCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
INALSVDVNPIPIKALVAYIGYANGELRLPLVPMLEQDTKQLIELYNRIAKGSDLS
HHHHCCCCCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNIHFKGTGVAVTTPFNGQTIDYNLFEDHLNFLINNNVEALIINGTTGEGSTLTEDEKLK
CEEEEECCCEEEECCCCCCEEEEHHHHHHHHEEECCCCEEEEEECCCCCCCCCCCCCCEE
TIEIAVRVAHGRVPVIAGTGTNNTQATIEHSLKAKALGVDSIMLITPYYNKTNQRGLLAH
EEEEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHH
FTTIADAVELPVLLYNVPARTNMTIEQETVAALAEHPYIYGIKDATGDINYMKTLKSVVP
HHHHHHHHHCCEEEEECCCCCCCEEHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCC
DDFALYSGNDDAVLPFYEAGGDGVISVIANAIPAEFSDIYRTYQVNRHEAERQFNNLLPL
CCEEEEECCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
INALSVDVNPIPIKALVAYIGYANGELRLPLVPMLEQDTKQLIELYNRIAKGSDLS
HHHHCCCCCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA