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The map label for this gene is glnA [H]

Identifier: 222151149

GI number: 222151149

Start: 994240

End: 995577

Strand: Direct

Name: glnA [H]

Synonym: MCCL_0900

Alternate gene names: 222151149

Gene position: 994240-995577 (Clockwise)

Preceding gene: 222151148

Following gene: 222151156

Centisome position: 47.29

GC content: 38.42

Gene sequence:

>1338_bases
ATGGCGAAATTTACAAGAGAAGACATCATCAAGTTAGCAGAGGAATCAAACGTACGTTATTTAAGACTACAATTTTCTGA
TATTCTAGGAACGATTAAAAACGTAGAGGTACCCATCAGCCAGTTAGAGAAAGTACTAGACAATGAAATGATGTTTGATG
GTTCATCAATCGAAGGGTTTGTACGTATCGAAGAATCAGATATGAAATTACATCCAGACTTAGACACATGGGTTATTTTC
CCTTGGACTTCAGACAAAGGTAAAGTCGCACGTTTAATCTGTGATGTGTATAAGCCAGACGGCACACCATTCTCAGGAGA
TCCACGTAGTAACTTAAAACGTGTATTGAAAGAAATGGAAGCTTTAGGATTTACAGACTTTAACTTAGGACCAGAACCAG
AATTCTTCTTATTCAAATTAGATGAAAAAGGGGAACCAACACTTGAATTAAATGATAATGGTGGATACTTCGATTTAGCG
CCTACAGATTTAGGTGAGAACTGTCGTCGAGACATCGTTTTAGAACTTGAAGACATGGGCTTTGATATAGAAGCAAGTCA
CCACGAAGTAGCGCCAGGTCAGCACGAAATCGACTTTAAATATGCAGATGCAGTTACTGCATGTGATAACATTCAAACAT
TCAAATTAGTTGTTAAGACAATTGCGCGTAAACATAATCTTCATGCAACATTTATGCCTAAACCATTATATGGTGTAAAT
GGTTCAGGTATGCACTTTAACGTTTCGTTATTCAAAGGACCGAAAGAAAATGCGTTCTATGATCCGAACGGAGAAATGGA
ATTATCTGAAGATGCGCGTCACTTTATCGCAGGAGTTCTTAAACATGCAAGAGGATTTACTGCAGTGTGTAACCCATTAG
TAAACTCTTACAAACGTTTAGTACCTGGTTATGAAGCACCAGTTTATATCGCATGGAGTGGCCAAAACCGTTCGCCATTA
ATTCGTATTCCTTCATCACGTGGACTATCTACGCGTGTAGAAGTACGTTCAGTTGATCCATCTGCTAACCCATATATGGC
ATTAGCAACGATTCTTCAAGCAGGATTAGATGGCATCAAGAATAAATTAGTTTGTCCAGCACCAGTTGATCAGAATATTT
ATGAAATGAATCGTGAAGAACGTGAATCTGTTGGTGTAGAAGACTTACCATCAACACTTTACACGGCAATCAAAGCGATG
AAAGAAGACGATATTTTAAAAGAAGCACTGGGTGACCACATCTATCGTGCATTCATCCATTCAAAAAATATTGAATGGGA
CATGTACCGTATGCAAGTGTCTGAGTGGGAACGTGGCCAATATATGAAGCAGTATTAA

Upstream 100 bases:

>100_bases
GAATCTCTTGATTACGAAGTTTCAAGGCTCGACCGTAGTAAAATCCCTATTAATCGTGGGGATCTGTCAAGATTTTTTAA
ATAAACAACTGGAGGATATT

Downstream 100 bases:

>100_bases
TTGCTTAAACCGTTGAAACTAAAATGTTTCGACGGTTTTTAATTTGTACCCAATTTGTAAAAAGCATCATAAAAATTATG
CTCTGGGTGTTTTTTGGGTA

Product: glutamine-ammonia ligase

Products: NA

Alternate protein names: GS; Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MAKFTREDIIKLAEESNVRYLRLQFSDILGTIKNVEVPISQLEKVLDNEMMFDGSSIEGFVRIEESDMKLHPDLDTWVIF
PWTSDKGKVARLICDVYKPDGTPFSGDPRSNLKRVLKEMEALGFTDFNLGPEPEFFLFKLDEKGEPTLELNDNGGYFDLA
PTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKLVVKTIARKHNLHATFMPKPLYGVN
GSGMHFNVSLFKGPKENAFYDPNGEMELSEDARHFIAGVLKHARGFTAVCNPLVNSYKRLVPGYEAPVYIAWSGQNRSPL
IRIPSSRGLSTRVEVRSVDPSANPYMALATILQAGLDGIKNKLVCPAPVDQNIYEMNREERESVGVEDLPSTLYTAIKAM
KEDDILKEALGDHIYRAFIHSKNIEWDMYRMQVSEWERGQYMKQY

Sequences:

>Translated_445_residues
MAKFTREDIIKLAEESNVRYLRLQFSDILGTIKNVEVPISQLEKVLDNEMMFDGSSIEGFVRIEESDMKLHPDLDTWVIF
PWTSDKGKVARLICDVYKPDGTPFSGDPRSNLKRVLKEMEALGFTDFNLGPEPEFFLFKLDEKGEPTLELNDNGGYFDLA
PTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKLVVKTIARKHNLHATFMPKPLYGVN
GSGMHFNVSLFKGPKENAFYDPNGEMELSEDARHFIAGVLKHARGFTAVCNPLVNSYKRLVPGYEAPVYIAWSGQNRSPL
IRIPSSRGLSTRVEVRSVDPSANPYMALATILQAGLDGIKNKLVCPAPVDQNIYEMNREERESVGVEDLPSTLYTAIKAM
KEDDILKEALGDHIYRAFIHSKNIEWDMYRMQVSEWERGQYMKQY
>Mature_444_residues
AKFTREDIIKLAEESNVRYLRLQFSDILGTIKNVEVPISQLEKVLDNEMMFDGSSIEGFVRIEESDMKLHPDLDTWVIFP
WTSDKGKVARLICDVYKPDGTPFSGDPRSNLKRVLKEMEALGFTDFNLGPEPEFFLFKLDEKGEPTLELNDNGGYFDLAP
TDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKLVVKTIARKHNLHATFMPKPLYGVNG
SGMHFNVSLFKGPKENAFYDPNGEMELSEDARHFIAGVLKHARGFTAVCNPLVNSYKRLVPGYEAPVYIAWSGQNRSPLI
RIPSSRGLSTRVEVRSVDPSANPYMALATILQAGLDGIKNKLVCPAPVDQNIYEMNREERESVGVEDLPSTLYTAIKAMK
EDDILKEALGDHIYRAFIHSKNIEWDMYRMQVSEWERGQYMKQY

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=393, Percent_Identity=27.4809160305344, Blast_Score=133, Evalue=3e-31,
Organism=Escherichia coli, GI1790301, Length=466, Percent_Identity=38.412017167382, Blast_Score=278, Evalue=6e-76,
Organism=Escherichia coli, GI87081870, Length=459, Percent_Identity=27.8867102396514, Blast_Score=150, Evalue=1e-37,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 50551; Mature: 50420

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKFTREDIIKLAEESNVRYLRLQFSDILGTIKNVEVPISQLEKVLDNEMMFDGSSIEGF
CCCCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHEEECCCCCCEE
VRIEESDMKLHPDLDTWVIFPWTSDKGKVARLICDVYKPDGTPFSGDPRSNLKRVLKEME
EEEECCCCEECCCCCEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
ALGFTDFNLGPEPEFFLFKLDEKGEPTLELNDNGGYFDLAPTDLGENCRRDIVLELEDMG
HCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCEEEECCCCCCCCCCCCEEEEEECCC
FDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKLVVKTIARKHNLHATFMPKPLYGVN
CEEECCCCCCCCCCCCCCEEEHHHHHHHCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
GSGMHFNVSLFKGPKENAFYDPNGEMELSEDARHFIAGVLKHARGFTAVCNPLVNSYKRL
CCCEEEEEEEECCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
VPGYEAPVYIAWSGQNRSPLIRIPSSRGLSTRVEVRSVDPSANPYMALATILQAGLDGIK
CCCCCCCEEEEECCCCCCCEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
NKLVCPAPVDQNIYEMNREERESVGVEDLPSTLYTAIKAMKEDDILKEALGDHIYRAFIH
CCEECCCCCCCHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKNIEWDMYRMQVSEWERGQYMKQY
CCCCCEEHEEEEHHHHHCCHHHHCC
>Mature Secondary Structure 
AKFTREDIIKLAEESNVRYLRLQFSDILGTIKNVEVPISQLEKVLDNEMMFDGSSIEGF
CCCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHEEECCCCCCEE
VRIEESDMKLHPDLDTWVIFPWTSDKGKVARLICDVYKPDGTPFSGDPRSNLKRVLKEME
EEEECCCCEECCCCCEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
ALGFTDFNLGPEPEFFLFKLDEKGEPTLELNDNGGYFDLAPTDLGENCRRDIVLELEDMG
HCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCEEEECCCCCCCCCCCCEEEEEECCC
FDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKLVVKTIARKHNLHATFMPKPLYGVN
CEEECCCCCCCCCCCCCCEEEHHHHHHHCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
GSGMHFNVSLFKGPKENAFYDPNGEMELSEDARHFIAGVLKHARGFTAVCNPLVNSYKRL
CCCEEEEEEEECCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
VPGYEAPVYIAWSGQNRSPLIRIPSSRGLSTRVEVRSVDPSANPYMALATILQAGLDGIK
CCCCCCCEEEEECCCCCCCEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
NKLVCPAPVDQNIYEMNREERESVGVEDLPSTLYTAIKAMKEDDILKEALGDHIYRAFIH
CCEECCCCCCCHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKNIEWDMYRMQVSEWERGQYMKQY
CCCCCEEHEEEEHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA