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The map label for this gene is glpK

Identifier: 222151141

GI number: 222151141

Start: 985341

End: 986834

Strand: Direct

Name: glpK

Synonym: MCCL_0892

Alternate gene names: 222151141

Gene position: 985341-986834 (Clockwise)

Preceding gene: 222151140

Following gene: 222151142

Centisome position: 46.87

GC content: 39.02

Gene sequence:

>1494_bases
ATGGAAAAATATATTTTATCAATTGATCAAGGGACAACAAGTACACGTGTAATCGTTTTTAATAAAGACGGAGAGATTAA
AGGGGTATCTCAAAGAGAATTTACTCAGCATTTTCCAAAGGCAGGCTGGGTTGAACATGATGCCAATGAAATTTGGTCAA
CTGTATTATCATGTTTTGCGTCCGTTCTTACAGAATCGAATATTAGGCCTGATCAGATTGCGGGTATTGGTATCACAAAT
CAACGTGAAACAGCAGTCGTATGGGATAAGAATACAGAGCGTCCAATCTATAACGCAATCGTATGGCAGTCTCGTCAAAC
TCAGGAAATCTGTAATGATCTTAAAGATAAAGGATTGGAAGATGAATTTAGAGAGAAGACTGGCCTTTTACTCGATCCTT
ATTTTTCGGGTACAAAAGTTAAATGGATTCTTGATAATGTAGAAGGTGCTAGAGAAAAAGCTGAGAATGGTGATCTATTA
TTTGGGACGATTGATTCATGGCTCGTATGGAAACTTTCTGGATGTAAAGAGCATGTAACTGACTACTCAAATGCAAGTCG
TACATTGATGTATAATATTCATGAATTGAAATGGGATGAACAGCTTCTTGAATATTTAACTGTACCTGCTTCAATGTTAC
CTGAAGTACGCCCATCAAGTGAAGTATATGGTAAAACAGCAAATCATCATTTCTTTGGTCATCAGATTCCGATTGCAGGT
ATTGCTGGTGATCAGCAGGCAGCGTTATTTGGGCAAGCATGCTTTGAGTCAGGAGAAGCTAAGAACACATACGGTACCGG
CTGCTTTATGTTAATGAACACAGGTGAAAAAGCAGTTAAAAGTGAAAATGGCTTATTAACGACTTTAGCTTATGGTATTG
ATGGCAAAGTCAACTACGCACTTGAAGGATCCATCTTTGTTGCGGGAAGTGCAATTCAATGGTTACGTGATGGTATGCGT
ATGATTCAAAGTGCTCCTCAAAGTGAAGAGTACGCTACAAATGTTACAGATGCTGATGGTGTGTATGTCGTTCCTGCATT
TGTTGGACTAGGTACACCTTACTGGGATTCTGAAGCACGTGGTGCAGTTTTCGGTTTAACGCGTGGTACACAAAAAGAAC
ATTTTATTCGTGCGACACTTGAGTCACTTGCATATCAGACTCGTGATGTTCTTGATGCGATGGAAAAAGATTCAAAGATT
GAAGTTAAAACATTAAGAGTTGATGGTGGTGCTGTCAAGAATGATTTCTTGATGCAGTTCCAGTCTGATATTTTAGATGT
ACCGGTAGAGCGACCTGAAATTAACGAGACAACTGCCTTAGGTGCAGCATACCTTGCTGGACTGGCAGTAGGTTACTGGA
AATCGAAAGATGAAATTCGTAATCGCTGGAACCTTGAAAAGCAGTTCGATCCAAAAATGGATGAAACAAAAAGAGAAGAT
CTATATAAAGGCTGGCAGACAGCGGTTAAAGCTACACAAGTATTTAAAAAATAA

Upstream 100 bases:

>100_bases
CGCATTAATTGTCGCTACATTAGTGCTTGGTGTATTCTTGAATAAAGCAGTGTTAAAGAATGAAAAAACTGACTTAATAT
AAAACAAAAGGGTGATGATT

Downstream 100 bases:

>100_bases
GTTTTAATGGAGGAATAATAATGCAACTTTCAAGTCTGAATAGAAATCAAACATTGAACAAAATGAAGGAAGAATATTAT
GATATCGTAGTGGTTGGTGG

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MEKYILSIDQGTTSTRVIVFNKDGEIKGVSQREFTQHFPKAGWVEHDANEIWSTVLSCFASVLTESNIRPDQIAGIGITN
QRETAVVWDKNTERPIYNAIVWQSRQTQEICNDLKDKGLEDEFREKTGLLLDPYFSGTKVKWILDNVEGAREKAENGDLL
FGTIDSWLVWKLSGCKEHVTDYSNASRTLMYNIHELKWDEQLLEYLTVPASMLPEVRPSSEVYGKTANHHFFGHQIPIAG
IAGDQQAALFGQACFESGEAKNTYGTGCFMLMNTGEKAVKSENGLLTTLAYGIDGKVNYALEGSIFVAGSAIQWLRDGMR
MIQSAPQSEEYATNVTDADGVYVVPAFVGLGTPYWDSEARGAVFGLTRGTQKEHFIRATLESLAYQTRDVLDAMEKDSKI
EVKTLRVDGGAVKNDFLMQFQSDILDVPVERPEINETTALGAAYLAGLAVGYWKSKDEIRNRWNLEKQFDPKMDETKRED
LYKGWQTAVKATQVFKK

Sequences:

>Translated_497_residues
MEKYILSIDQGTTSTRVIVFNKDGEIKGVSQREFTQHFPKAGWVEHDANEIWSTVLSCFASVLTESNIRPDQIAGIGITN
QRETAVVWDKNTERPIYNAIVWQSRQTQEICNDLKDKGLEDEFREKTGLLLDPYFSGTKVKWILDNVEGAREKAENGDLL
FGTIDSWLVWKLSGCKEHVTDYSNASRTLMYNIHELKWDEQLLEYLTVPASMLPEVRPSSEVYGKTANHHFFGHQIPIAG
IAGDQQAALFGQACFESGEAKNTYGTGCFMLMNTGEKAVKSENGLLTTLAYGIDGKVNYALEGSIFVAGSAIQWLRDGMR
MIQSAPQSEEYATNVTDADGVYVVPAFVGLGTPYWDSEARGAVFGLTRGTQKEHFIRATLESLAYQTRDVLDAMEKDSKI
EVKTLRVDGGAVKNDFLMQFQSDILDVPVERPEINETTALGAAYLAGLAVGYWKSKDEIRNRWNLEKQFDPKMDETKRED
LYKGWQTAVKATQVFKK
>Mature_497_residues
MEKYILSIDQGTTSTRVIVFNKDGEIKGVSQREFTQHFPKAGWVEHDANEIWSTVLSCFASVLTESNIRPDQIAGIGITN
QRETAVVWDKNTERPIYNAIVWQSRQTQEICNDLKDKGLEDEFREKTGLLLDPYFSGTKVKWILDNVEGAREKAENGDLL
FGTIDSWLVWKLSGCKEHVTDYSNASRTLMYNIHELKWDEQLLEYLTVPASMLPEVRPSSEVYGKTANHHFFGHQIPIAG
IAGDQQAALFGQACFESGEAKNTYGTGCFMLMNTGEKAVKSENGLLTTLAYGIDGKVNYALEGSIFVAGSAIQWLRDGMR
MIQSAPQSEEYATNVTDADGVYVVPAFVGLGTPYWDSEARGAVFGLTRGTQKEHFIRATLESLAYQTRDVLDAMEKDSKI
EVKTLRVDGGAVKNDFLMQFQSDILDVPVERPEINETTALGAAYLAGLAVGYWKSKDEIRNRWNLEKQFDPKMDETKRED
LYKGWQTAVKATQVFKK

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI189409120, Length=496, Percent_Identity=46.5725806451613, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI4504007, Length=496, Percent_Identity=46.5725806451613, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI42794763, Length=500, Percent_Identity=46.2, Blast_Score=435, Evalue=1e-122,
Organism=Homo sapiens, GI41393575, Length=496, Percent_Identity=45.9677419354839, Blast_Score=434, Evalue=1e-122,
Organism=Homo sapiens, GI88196792, Length=505, Percent_Identity=31.8811881188119, Blast_Score=305, Evalue=8e-83,
Organism=Escherichia coli, GI1790361, Length=489, Percent_Identity=64.4171779141104, Blast_Score=669, Evalue=0.0,
Organism=Escherichia coli, GI1790005, Length=523, Percent_Identity=23.1357552581262, Blast_Score=113, Evalue=4e-26,
Organism=Escherichia coli, GI1789987, Length=486, Percent_Identity=24.0740740740741, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1789168, Length=494, Percent_Identity=25.1012145748988, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787789, Length=520, Percent_Identity=24.0384615384615, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789138, Length=520, Percent_Identity=23.0769230769231, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17535599, Length=497, Percent_Identity=42.6559356136821, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI71986140, Length=515, Percent_Identity=25.631067961165, Blast_Score=186, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI71986147, Length=262, Percent_Identity=31.6793893129771, Blast_Score=135, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6321755, Length=553, Percent_Identity=34.9005424954792, Blast_Score=310, Evalue=2e-85,
Organism=Drosophila melanogaster, GI17864214, Length=500, Percent_Identity=43.2, Blast_Score=404, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24654573, Length=429, Percent_Identity=44.7552447552448, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24655555, Length=496, Percent_Identity=37.9032258064516, Blast_Score=359, Evalue=3e-99,
Organism=Drosophila melanogaster, GI24655560, Length=496, Percent_Identity=37.9032258064516, Blast_Score=359, Evalue=3e-99,
Organism=Drosophila melanogaster, GI24655570, Length=496, Percent_Identity=37.9032258064516, Blast_Score=359, Evalue=3e-99,
Organism=Drosophila melanogaster, GI24655574, Length=496, Percent_Identity=37.9032258064516, Blast_Score=359, Evalue=3e-99,
Organism=Drosophila melanogaster, GI24655565, Length=496, Percent_Identity=37.9032258064516, Blast_Score=359, Evalue=3e-99,
Organism=Drosophila melanogaster, GI19922058, Length=522, Percent_Identity=31.8007662835249, Blast_Score=282, Evalue=4e-76,
Organism=Drosophila melanogaster, GI24652871, Length=489, Percent_Identity=32.3108384458078, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24656367, Length=493, Percent_Identity=26.5720081135903, Blast_Score=212, Evalue=5e-55,
Organism=Drosophila melanogaster, GI21357867, Length=508, Percent_Identity=25.7874015748031, Blast_Score=204, Evalue=8e-53,
Organism=Drosophila melanogaster, GI24656372, Length=328, Percent_Identity=28.9634146341463, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI45552909, Length=290, Percent_Identity=26.551724137931, Blast_Score=125, Evalue=8e-29,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_MACCJ (B9EBI8)

Other databases:

- EMBL:   AP009484
- RefSeq:   YP_002560295.1
- ProteinModelPortal:   B9EBI8
- GeneID:   7391097
- GenomeReviews:   AP009484_GR
- KEGG:   mcl:MCCL_0892
- OMA:   KPSSEVY
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 55809; Mature: 55809

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 12-12 BINDING 16-16 BINDING 82-82 BINDING 134-134 BINDING 244-244 BINDING 266-266 BINDING 309-309

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKYILSIDQGTTSTRVIVFNKDGEIKGVSQREFTQHFPKAGWVEHDANEIWSTVLSCFA
CCCEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
SVLTESNIRPDQIAGIGITNQRETAVVWDKNTERPIYNAIVWQSRQTQEICNDLKDKGLE
HHHHCCCCCHHHEEECCCCCCCCEEEEECCCCCCCHHHHHHCCCCCHHHHHHHHHHCCCC
DEFREKTGLLLDPYFSGTKVKWILDNVEGAREKAENGDLLFGTIDSWLVWKLSGCKEHVT
HHHHHHCCCEEECCCCCCEEEEEEECCHHHHHHCCCCCEEEEECCCEEEEEECCHHHHHH
DYSNASRTLMYNIHELKWDEQLLEYLTVPASMLPEVRPSSEVYGKTANHHFFGHQIPIAG
CCCCCCCEEEEEHHHHCHHHHHHHHHHCCHHHCCCCCCCHHHCCCCCCCCCCCCCCEEEE
IAGDQQAALFGQACFESGEAKNTYGTGCFMLMNTGEKAVKSENGLLTTLAYGIDGKVNYA
ECCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCHHHHHCCCCEEEEEEECCCCEEEEE
LEGSIFVAGSAIQWLRDGMRMIQSAPQSEEYATNVTDADGVYVVPAFVGLGTPYWDSEAR
EECCEEEECHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCEEEEHHHHCCCCCCCCCCC
GAVFGLTRGTQKEHFIRATLESLAYQTRDVLDAMEKDSKIEVKTLRVDGGAVKNDFLMQF
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHH
QSDILDVPVERPEINETTALGAAYLAGLAVGYWKSKDEIRNRWNLEKQFDPKMDETKRED
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCHHHHHH
LYKGWQTAVKATQVFKK
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEKYILSIDQGTTSTRVIVFNKDGEIKGVSQREFTQHFPKAGWVEHDANEIWSTVLSCFA
CCCEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
SVLTESNIRPDQIAGIGITNQRETAVVWDKNTERPIYNAIVWQSRQTQEICNDLKDKGLE
HHHHCCCCCHHHEEECCCCCCCCEEEEECCCCCCCHHHHHHCCCCCHHHHHHHHHHCCCC
DEFREKTGLLLDPYFSGTKVKWILDNVEGAREKAENGDLLFGTIDSWLVWKLSGCKEHVT
HHHHHHCCCEEECCCCCCEEEEEEECCHHHHHHCCCCCEEEEECCCEEEEEECCHHHHHH
DYSNASRTLMYNIHELKWDEQLLEYLTVPASMLPEVRPSSEVYGKTANHHFFGHQIPIAG
CCCCCCCEEEEEHHHHCHHHHHHHHHHCCHHHCCCCCCCHHHCCCCCCCCCCCCCCEEEE
IAGDQQAALFGQACFESGEAKNTYGTGCFMLMNTGEKAVKSENGLLTTLAYGIDGKVNYA
ECCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCHHHHHCCCCEEEEEEECCCCEEEEE
LEGSIFVAGSAIQWLRDGMRMIQSAPQSEEYATNVTDADGVYVVPAFVGLGTPYWDSEAR
EECCEEEECHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCEEEEHHHHCCCCCCCCCCC
GAVFGLTRGTQKEHFIRATLESLAYQTRDVLDAMEKDSKIEVKTLRVDGGAVKNDFLMQF
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHH
QSDILDVPVERPEINETTALGAAYLAGLAVGYWKSKDEIRNRWNLEKQFDPKMDETKRED
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCHHHHHH
LYKGWQTAVKATQVFKK
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA