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The map label for this gene is glpF [H]

Identifier: 222151140

GI number: 222151140

Start: 984504

End: 985322

Strand: Direct

Name: glpF [H]

Synonym: MCCL_0891

Alternate gene names: 222151140

Gene position: 984504-985322 (Clockwise)

Preceding gene: 222151139

Following gene: 222151141

Centisome position: 46.83

GC content: 40.66

Gene sequence:

>819_bases
ATGAATGCTTTTGTGGGAGAATTAATCGGTACTGCAATCTTAATTTTATTTGGTTGTGGTGTAAATGCGAACGTTAATTT
AAAGGGGACGTACGCAAAAGGCAGTGACTGGATTGTTATCGCAGTAGGTTGGGGTCTTGCTGTTGTTATGGGTGTGTATG
CAGTTGGATCAATTACTGGTGCGCATTTAAATCCAGCAGTTACAATAGGAATGGCAGTTGATGGGAGCCTTCCGTGGAAT
CAAGTCCTTCCGTATTTTGCAGGGCAGATGATTGGCGGGGTACTTGGAGCAGCATTAGTATGGTTCCAGTTTATGCCGCA
TTTTAAAGAAGAAGATGATGCAGGTACTAAACTTGGCGTATTTGCTACAGGACCAGCTTATCCTAACTATGTTTCTAACT
TTGTATCGGAAATCATAGGAACTTCTATATTGGTTATGGCACTGTTATTCATTGGAGGAAATAAATTCTCTGAAGGGTTG
AATCCTCTTGTAGTAGGGCTATTGATTATAGCCATCGGAGTAAGTTTAGGTGGAACAACTGGTTATGCAATCAATCCGGC
ACGTGACTGGGGTCCGCGTATCGCTCATACGATTCTACCGATACCAGGTAAAGGGTCTTCCAACTGGAAATATGCAGTGG
TTCCAATGGTAGGTCCGATGGTAGGTGGTGTCTTCGGCGCTACATTATTCCATGTTTTATTTAAGGAAGATCAATATATG
TTATTTGGTTTATCAATCGCATTAATTGTCGCTACATTAGTGCTTGGTGTATTCTTGAATAAAGCAGTGTTAAAGAATGA
AAAAACTGACTTAATATAA

Upstream 100 bases:

>100_bases
TTAAGTTAATATTTGGGACGAGTAGAAGAGACCTCTAATATAAAGTATTAGGGGTCTCTTCGTCTTTTTTTAACTTAACT
AAACAAAGAGGAGTGGGTCT

Downstream 100 bases:

>100_bases
AACAAAAGGGTGATGATTATGGAAAAATATATTTTATCAATTGATCAAGGGACAACAAGTACACGTGTAATCGTTTTTAA
TAAAGACGGAGAGATTAAAG

Product: glycerol uptake facilitator

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MNAFVGELIGTAILILFGCGVNANVNLKGTYAKGSDWIVIAVGWGLAVVMGVYAVGSITGAHLNPAVTIGMAVDGSLPWN
QVLPYFAGQMIGGVLGAALVWFQFMPHFKEEDDAGTKLGVFATGPAYPNYVSNFVSEIIGTSILVMALLFIGGNKFSEGL
NPLVVGLLIIAIGVSLGGTTGYAINPARDWGPRIAHTILPIPGKGSSNWKYAVVPMVGPMVGGVFGATLFHVLFKEDQYM
LFGLSIALIVATLVLGVFLNKAVLKNEKTDLI

Sequences:

>Translated_272_residues
MNAFVGELIGTAILILFGCGVNANVNLKGTYAKGSDWIVIAVGWGLAVVMGVYAVGSITGAHLNPAVTIGMAVDGSLPWN
QVLPYFAGQMIGGVLGAALVWFQFMPHFKEEDDAGTKLGVFATGPAYPNYVSNFVSEIIGTSILVMALLFIGGNKFSEGL
NPLVVGLLIIAIGVSLGGTTGYAINPARDWGPRIAHTILPIPGKGSSNWKYAVVPMVGPMVGGVFGATLFHVLFKEDQYM
LFGLSIALIVATLVLGVFLNKAVLKNEKTDLI
>Mature_272_residues
MNAFVGELIGTAILILFGCGVNANVNLKGTYAKGSDWIVIAVGWGLAVVMGVYAVGSITGAHLNPAVTIGMAVDGSLPWN
QVLPYFAGQMIGGVLGAALVWFQFMPHFKEEDDAGTKLGVFATGPAYPNYVSNFVSEIIGTSILVMALLFIGGNKFSEGL
NPLVVGLLIIAIGVSLGGTTGYAINPARDWGPRIAHTILPIPGKGSSNWKYAVVPMVGPMVGGVFGATLFHVLFKEDQYM
LFGLSIALIVATLVLGVFLNKAVLKNEKTDLI

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=248, Percent_Identity=34.6774193548387, Blast_Score=124, Evalue=9e-29,
Organism=Homo sapiens, GI157266307, Length=255, Percent_Identity=34.1176470588235, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI4502187, Length=248, Percent_Identity=34.2741935483871, Blast_Score=107, Evalue=8e-24,
Organism=Homo sapiens, GI22538420, Length=245, Percent_Identity=31.0204081632653, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI310133356, Length=206, Percent_Identity=33.0097087378641, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI310114181, Length=206, Percent_Identity=33.0097087378641, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI45446752, Length=189, Percent_Identity=31.2169312169312, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4755125, Length=246, Percent_Identity=26.0162601626016, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI4502181, Length=246, Percent_Identity=26.0162601626016, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI4502179, Length=258, Percent_Identity=28.2945736434109, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1790362, Length=246, Percent_Identity=35.7723577235772, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1787101, Length=186, Percent_Identity=31.1827956989247, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI71994009, Length=233, Percent_Identity=34.3347639484979, Blast_Score=114, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI71992966, Length=248, Percent_Identity=29.8387096774194, Blast_Score=107, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17531429, Length=243, Percent_Identity=32.9218106995885, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17544068, Length=248, Percent_Identity=34.2741935483871, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17531431, Length=243, Percent_Identity=32.9218106995885, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17533613, Length=238, Percent_Identity=28.5714285714286, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI32564052, Length=238, Percent_Identity=28.5714285714286, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71992961, Length=243, Percent_Identity=27.9835390946502, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17558372, Length=204, Percent_Identity=29.4117647058824, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71993722, Length=201, Percent_Identity=26.3681592039801, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321054, Length=208, Percent_Identity=37.5, Blast_Score=123, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6322985, Length=283, Percent_Identity=29.3286219081272, Blast_Score=92, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24652747, Length=234, Percent_Identity=29.4871794871795, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45551084, Length=234, Percent_Identity=29.4871794871795, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24762342, Length=258, Percent_Identity=25.968992248062, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24762344, Length=254, Percent_Identity=26.3779527559055, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24762346, Length=254, Percent_Identity=26.3779527559055, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24762348, Length=254, Percent_Identity=26.3779527559055, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI20130305, Length=254, Percent_Identity=26.3779527559055, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 28531; Mature: 28531

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAFVGELIGTAILILFGCGVNANVNLKGTYAKGSDWIVIAVGWGLAVVMGVYAVGSITG
CCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHCCC
AHLNPAVTIGMAVDGSLPWNQVLPYFAGQMIGGVLGAALVWFQFMPHFKEEDDAGTKLGV
CCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
FATGPAYPNYVSNFVSEIIGTSILVMALLFIGGNKFSEGLNPLVVGLLIIAIGVSLGGTT
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHCCCCC
GYAINPARDWGPRIAHTILPIPGKGSSNWKYAVVPMVGPMVGGVFGATLFHVLFKEDQYM
CCEECCCHHCCCHHHHHEECCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCEE
LFGLSIALIVATLVLGVFLNKAVLKNEKTDLI
EHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNAFVGELIGTAILILFGCGVNANVNLKGTYAKGSDWIVIAVGWGLAVVMGVYAVGSITG
CCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHCCC
AHLNPAVTIGMAVDGSLPWNQVLPYFAGQMIGGVLGAALVWFQFMPHFKEEDDAGTKLGV
CCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
FATGPAYPNYVSNFVSEIIGTSILVMALLFIGGNKFSEGLNPLVVGLLIIAIGVSLGGTT
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHCCCCC
GYAINPARDWGPRIAHTILPIPGKGSSNWKYAVVPMVGPMVGGVFGATLFHVLFKEDQYM
CCEECCCHHCCCHHHHHEECCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCEE
LFGLSIALIVATLVLGVFLNKAVLKNEKTDLI
EHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8436953; 9579061; 9384377; 2127799 [H]