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The map label for this gene is murE [H]

Identifier: 222150881

GI number: 222150881

Start: 675031

End: 676500

Strand: Direct

Name: murE [H]

Synonym: MCCL_0631

Alternate gene names: 222150881

Gene position: 675031-676500 (Clockwise)

Preceding gene: 222150878

Following gene: 222150882

Centisome position: 32.11

GC content: 43.54

Gene sequence:

>1470_bases
GTGAAAACGTCTGAATTACTTTCTAAAATAAAAGCCAAGAAGCTTTACGGAACATTTCCTGAACATATCAATAAAGTGGT
TGTAGATTCAAGAGCTGCAGACAGCGAGAGTGTATTTGTAGCAAGTCATGGGTATACAGTAGACAGCCATAAATTTATAC
CGAATGTAGTTAGACAGGGCTGTCGTATGATTGTCGTCGATCACTATATCGAAGGACTGGACATCGGGCAGGTTGTCGTT
CGTGATACTTTGCAGGCAGCTCGTATATTCACGCAGCATGTAATGGATTTCCCGTCACATAAGCTTACGACTTATGGTGT
GACAGGTACGAACGGTAAGACGAGTGTGGCAACGATGATTCATCATCTGCACCGCGCATTGAACATAAATAGTGCATATC
TCGGTACAAATGGCTTTCAATATAATGAAGAAGTTGCTAAAGGTGCGAATTCAACCCCTGAAACGATTACCTTAAATCAG
CATATCGCACATGCAGTAGAACAAGGTGCACAGTCTATGGCGATGGAAATGTCGAGCCATGGATTAGCACTTGGTCGTAC
GGATGGCGTTGACATCGATGTTGCAATCTTCACGAACTTGACGCAAGATCATCTGGATTTTCATGGTACGATGGAACGTT
ATGGCTTTCATAAGGCGCTGCTCTTTGCACAGCTTGGGAATGACTTTAAGCAGCAGAAGTATGCAGTGGTAAATGGAGAT
GATGATTATTCGAAAGAACTGATCATTGCTTCACCAAGAGAAGTGATTACATATGGCATTGAACAGAATACAGATATTAA
AGCAACAAATATTAAAGAATCTATCCATGGCATTGCCTTTACACTTGAAACACCAGATGGCAATGTTGAGATAACTTGTC
CTTATATTGGTCGATTCAACATCTATAATATGCTTGCAGCCCTCACCGCACTGTGGACACAAGGTCATGCACTGAGTGCA
CTTGCTGAAGCAGCACAAAGTTTACCACCAGTAGAGGGCCGTCTAGAAGTGCTTGACCGCAACTTACCGATAGACTTAGT
GATTGATTATGCACATACGCCAGACGGCATGAACAAACTTATCGATGCACTGGAACCATTCAAGCGCGGGAAGATGATTT
TCTTAATTGGTATGGCAGGAGAACGTGATTTGTCGAAGACGCCTGAGATGGGAGCGGTTGCTTGTCGTGCAGACTATGTC
ATCTTTACACCAGATAATCCGGCAAATGATGACCCGCGTAAGCTTACGGATGCACTTGAAAGTGGTGCCACACATACGAA
CTACGTATCATTTCTTGACCGTGCAGAAGGGATTCGTCACGCGATTGCCATGAGTGAGCCAGGAGATATGGTCGTTTTAG
CCTCAAAAGGTCGTGAGCCTTATCAGATTATGGAAAATTACATTAAAGTACCGCATCGTGATGACTTGATCGGTCTGGAA
GCGGCATATGATAAATACGGGAGAGAGTAA

Upstream 100 bases:

>100_bases
CTTCTGTTTTATACAAATTATTTGTTTAAACCTATCTTCAACAATTCATAATCCTATGAAAAAGGTGTATAATTAAGAAT
TGACTAAAGGAGGATTATCA

Downstream 100 bases:

>100_bases
CGATGAAAATTAGAAACAGTATAGGAGAAAACCTGCAATGTAAAGTATATATTCATGAAAACAAAAAGGAAGAAACGTTT
TTAGTAAGTATACCGGATAT

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--L-lysine ligase

Products: NA

Alternate protein names: L-lysine-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:L-Lys ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MKTSELLSKIKAKKLYGTFPEHINKVVVDSRAADSESVFVASHGYTVDSHKFIPNVVRQGCRMIVVDHYIEGLDIGQVVV
RDTLQAARIFTQHVMDFPSHKLTTYGVTGTNGKTSVATMIHHLHRALNINSAYLGTNGFQYNEEVAKGANSTPETITLNQ
HIAHAVEQGAQSMAMEMSSHGLALGRTDGVDIDVAIFTNLTQDHLDFHGTMERYGFHKALLFAQLGNDFKQQKYAVVNGD
DDYSKELIIASPREVITYGIEQNTDIKATNIKESIHGIAFTLETPDGNVEITCPYIGRFNIYNMLAALTALWTQGHALSA
LAEAAQSLPPVEGRLEVLDRNLPIDLVIDYAHTPDGMNKLIDALEPFKRGKMIFLIGMAGERDLSKTPEMGAVACRADYV
IFTPDNPANDDPRKLTDALESGATHTNYVSFLDRAEGIRHAIAMSEPGDMVVLASKGREPYQIMENYIKVPHRDDLIGLE
AAYDKYGRE

Sequences:

>Translated_489_residues
MKTSELLSKIKAKKLYGTFPEHINKVVVDSRAADSESVFVASHGYTVDSHKFIPNVVRQGCRMIVVDHYIEGLDIGQVVV
RDTLQAARIFTQHVMDFPSHKLTTYGVTGTNGKTSVATMIHHLHRALNINSAYLGTNGFQYNEEVAKGANSTPETITLNQ
HIAHAVEQGAQSMAMEMSSHGLALGRTDGVDIDVAIFTNLTQDHLDFHGTMERYGFHKALLFAQLGNDFKQQKYAVVNGD
DDYSKELIIASPREVITYGIEQNTDIKATNIKESIHGIAFTLETPDGNVEITCPYIGRFNIYNMLAALTALWTQGHALSA
LAEAAQSLPPVEGRLEVLDRNLPIDLVIDYAHTPDGMNKLIDALEPFKRGKMIFLIGMAGERDLSKTPEMGAVACRADYV
IFTPDNPANDDPRKLTDALESGATHTNYVSFLDRAEGIRHAIAMSEPGDMVVLASKGREPYQIMENYIKVPHRDDLIGLE
AAYDKYGRE
>Mature_489_residues
MKTSELLSKIKAKKLYGTFPEHINKVVVDSRAADSESVFVASHGYTVDSHKFIPNVVRQGCRMIVVDHYIEGLDIGQVVV
RDTLQAARIFTQHVMDFPSHKLTTYGVTGTNGKTSVATMIHHLHRALNINSAYLGTNGFQYNEEVAKGANSTPETITLNQ
HIAHAVEQGAQSMAMEMSSHGLALGRTDGVDIDVAIFTNLTQDHLDFHGTMERYGFHKALLFAQLGNDFKQQKYAVVNGD
DDYSKELIIASPREVITYGIEQNTDIKATNIKESIHGIAFTLETPDGNVEITCPYIGRFNIYNMLAALTALWTQGHALSA
LAEAAQSLPPVEGRLEVLDRNLPIDLVIDYAHTPDGMNKLIDALEPFKRGKMIFLIGMAGERDLSKTPEMGAVACRADYV
IFTPDNPANDDPRKLTDALESGATHTNYVSFLDRAEGIRHAIAMSEPGDMVVLASKGREPYQIMENYIKVPHRDDLIGLE
AAYDKYGRE

Specific function: Catalyzes the addition of L-lysine to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=466, Percent_Identity=31.7596566523605, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI1786274, Length=317, Percent_Identity=27.4447949526814, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.7 [H]

Molecular weight: Translated: 54024; Mature: 54024

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSELLSKIKAKKLYGTFPEHINKVVVDSRAADSESVFVASHGYTVDSHKFIPNVVRQG
CCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEECCEECCCCHHHHHHHHCC
CRMIVVDHYIEGLDIGQVVVRDTLQAARIFTQHVMDFPSHKLTTYGVTGTNGKTSVATMI
CEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHH
HHLHRALNINSAYLGTNGFQYNEEVAKGANSTPETITLNQHIAHAVEQGAQSMAMEMSSH
HHHHHHHCCCCEEECCCCCCCCHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHC
GLALGRTDGVDIDVAIFTNLTQDHLDFHGTMERYGFHKALLFAQLGNDFKQQKYAVVNGD
CEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCEEEEECCC
DDYSKELIIASPREVITYGIEQNTDIKATNIKESIHGIAFTLETPDGNVEITCPYIGRFN
CCCCCCEEEECCHHHEEECCCCCCCEEECCHHHHHCEEEEEEECCCCCEEEEECCCCCCH
IYNMLAALTALWTQGHALSALAEAAQSLPPVEGRLEVLDRNLPIDLVIDYAHTPDGMNKL
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCEEEEEEECCCCCHHHHH
IDALEPFKRGKMIFLIGMAGERDLSKTPEMGAVACRADYVIFTPDNPANDDPRKLTDALE
HHHHHHHHCCCEEEEEEECCCCCCCCCCCCCEEEEECCEEEECCCCCCCCCHHHHHHHHH
SGATHTNYVSFLDRAEGIRHAIAMSEPGDMVVLASKGREPYQIMENYIKVPHRDDLIGLE
CCCCHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCCCHHHHHHHHHCCCCCCCEEEHH
AAYDKYGRE
HHHHHCCCC
>Mature Secondary Structure
MKTSELLSKIKAKKLYGTFPEHINKVVVDSRAADSESVFVASHGYTVDSHKFIPNVVRQG
CCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEECCEECCCCHHHHHHHHCC
CRMIVVDHYIEGLDIGQVVVRDTLQAARIFTQHVMDFPSHKLTTYGVTGTNGKTSVATMI
CEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHH
HHLHRALNINSAYLGTNGFQYNEEVAKGANSTPETITLNQHIAHAVEQGAQSMAMEMSSH
HHHHHHHCCCCEEECCCCCCCCHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHC
GLALGRTDGVDIDVAIFTNLTQDHLDFHGTMERYGFHKALLFAQLGNDFKQQKYAVVNGD
CEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCEEEEECCC
DDYSKELIIASPREVITYGIEQNTDIKATNIKESIHGIAFTLETPDGNVEITCPYIGRFN
CCCCCCEEEECCHHHEEECCCCCCCEEECCHHHHHCEEEEEEECCCCCEEEEECCCCCCH
IYNMLAALTALWTQGHALSALAEAAQSLPPVEGRLEVLDRNLPIDLVIDYAHTPDGMNKL
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCEEEEEEECCCCCHHHHH
IDALEPFKRGKMIFLIGMAGERDLSKTPEMGAVACRADYVIFTPDNPANDDPRKLTDALE
HHHHHHHHCCCEEEEEEECCCCCCCCCCCCCEEEEECCEEEECCCCCCCCCHHHHHHHHH
SGATHTNYVSFLDRAEGIRHAIAMSEPGDMVVLASKGREPYQIMENYIKVPHRDDLIGLE
CCCCHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCCCHHHHHHHHHCCCCCCCEEEHH
AAYDKYGRE
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA