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The map label for this gene is prfC

Identifier: 222150882

GI number: 222150882

Start: 676754

End: 678316

Strand: Direct

Name: prfC

Synonym: MCCL_0632

Alternate gene names: 222150882

Gene position: 676754-678316 (Clockwise)

Preceding gene: 222150881

Following gene: 222150883

Centisome position: 32.19

GC content: 42.67

Gene sequence:

>1563_bases
ATGACATTAAGAGAAGAAGTAGAAATAAGAAAGACATTTGCCATCATCTCTCACCCGGATGCCGGTAAGACCACATTAAC
GGAGAAGTTATTATTATTCGGTGGCGCCATTCGTGAAGCAGGTACCGTCAAGGGTAAGAAGACCGGTAAGTTTGCGACAA
GTGACTGGATGGAAGTAGAAAAGCAGCGTGGAATTTCTGTAACGAGTTCTGTCATGCAGTTTGATTATGATAACTTTAAA
ATCAACATATTGGATACACCAGGGCATGAAGATTTCTCGGAAGATACGTATCGTACGTTAATGGCGGTGGATAGTGCTGT
CATGGTGATCGACTGCGCGAAAGGGATCGAGCCGCAGACGTTAAAGTTATTTAAAGTCTGTAAGATGCGTGGGATTCCGA
TATTCACATTCATCAATAAGCTTGACCGTGTCGGTAAGGAGCCATTTGAACTGCTGGAAGAAATCGAGAAGACACTTGAA
ATAGAGACGTATCCGATGAACTGGCCAATCGGTATGGGGCAATCATTCTTCGGTATCATTGACCGTAAGACAAAAACGAT
TGAGCCGTTTAGAGATGAAGAGAACGTACTGCATTTAAATGAGGATTATGAGCTTCAGGAAAGTCATGCAATCACGTCAG
ATAGCGCATATGAACAGGCGATTGAAGAGTTGATGCTTGTGGATGAAGCAGGCGAGACGTTTGATAAGGAGAAACTGATG
ACAGGTGATCTAACACCCGTGTTCTTCGGCTCAGCACTTGCAAACTTCGGTGTACAGAACTTCCTGAATGCATATGTAGA
TCATGCGCCGATGCCATCTGGACGTAAAACGGAGTCAGGTGAAGAAATATCACCATTTGATGAAAGTTTCAGTGGATTTA
TCTTCAAGATTCAGGCGAATATGAACCCGCAGCATAGAGATAGAATCGCCTTCATGCGTATCGTGAGTGGTGCATTTGAA
CGTGGCATGGACATTAAGATGACACGTACAGATAAGAAGATGAAGATTTCACGTTCAACATCGTTTATGGCAGATGATAC
ACAGACGGTAAACCATGCAGTGAGTGGCGATATTATCGGACTATATGACTCTGGGAACTTCCAGATCGGTGATACACTTG
TCGGTGGTAACCAGAAGTTCCAGTTTGAGAAATTACCTCAGTTCACACCAGAAATCTTTATGAAAGTGTCACCGAAGAAC
GTAATGAAGCAGAAGCATTTCCATAAAGGAATTGAACAGCTTGTCCAGGAAGGTGCCATTCAGCTTTATCGTACACTGCA
TACGAATCAGATTATCTTAGGGGCAGTCGGACAGCTTCAGTTTGAAGTATTTGAACATCGTATGAATAACGAATACAATG
TAGACGTCATCATGGAACCTGTAGGCAGAAAGATTGCCCGCTGGATTGAGAACGAAGCAGATATTCGTGATGCAATGAAC
TCAAGTCGTTCGATACTTGTTGAAGATAGGTTTGAAAACAAAGTATTCTTATTTGAGAATGAATTTGCGACACGCTGGTT
CCTGGATAAGTTCCCGGAAATTAAGCTCTACAGCTTACTGTAA

Upstream 100 bases:

>100_bases
ATCTATATTTACATTTATTTAATTTATTAGACGAAAAAGATGCATCTGAACTTGCACTTCGCATAGTGCAATGGACGAGC
GAAACTTAAGGAGCGAATTT

Downstream 100 bases:

>100_bases
GAAGGAGGAGTTATAGTGGATCCGTCAATTATCATCACCTATCTATGGGTTGTACTTGTACTCGTTGTATTAGAAGGCCT
GCTTGCTGCAGATAACGCTA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MTLREEVEIRKTFAIISHPDAGKTTLTEKLLLFGGAIREAGTVKGKKTGKFATSDWMEVEKQRGISVTSSVMQFDYDNFK
INILDTPGHEDFSEDTYRTLMAVDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLEEIEKTLE
IETYPMNWPIGMGQSFFGIIDRKTKTIEPFRDEENVLHLNEDYELQESHAITSDSAYEQAIEELMLVDEAGETFDKEKLM
TGDLTPVFFGSALANFGVQNFLNAYVDHAPMPSGRKTESGEEISPFDESFSGFIFKIQANMNPQHRDRIAFMRIVSGAFE
RGMDIKMTRTDKKMKISRSTSFMADDTQTVNHAVSGDIIGLYDSGNFQIGDTLVGGNQKFQFEKLPQFTPEIFMKVSPKN
VMKQKHFHKGIEQLVQEGAIQLYRTLHTNQIILGAVGQLQFEVFEHRMNNEYNVDVIMEPVGRKIARWIENEADIRDAMN
SSRSILVEDRFENKVFLFENEFATRWFLDKFPEIKLYSLL

Sequences:

>Translated_520_residues
MTLREEVEIRKTFAIISHPDAGKTTLTEKLLLFGGAIREAGTVKGKKTGKFATSDWMEVEKQRGISVTSSVMQFDYDNFK
INILDTPGHEDFSEDTYRTLMAVDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLEEIEKTLE
IETYPMNWPIGMGQSFFGIIDRKTKTIEPFRDEENVLHLNEDYELQESHAITSDSAYEQAIEELMLVDEAGETFDKEKLM
TGDLTPVFFGSALANFGVQNFLNAYVDHAPMPSGRKTESGEEISPFDESFSGFIFKIQANMNPQHRDRIAFMRIVSGAFE
RGMDIKMTRTDKKMKISRSTSFMADDTQTVNHAVSGDIIGLYDSGNFQIGDTLVGGNQKFQFEKLPQFTPEIFMKVSPKN
VMKQKHFHKGIEQLVQEGAIQLYRTLHTNQIILGAVGQLQFEVFEHRMNNEYNVDVIMEPVGRKIARWIENEADIRDAMN
SSRSILVEDRFENKVFLFENEFATRWFLDKFPEIKLYSLL
>Mature_519_residues
TLREEVEIRKTFAIISHPDAGKTTLTEKLLLFGGAIREAGTVKGKKTGKFATSDWMEVEKQRGISVTSSVMQFDYDNFKI
NILDTPGHEDFSEDTYRTLMAVDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLEEIEKTLEI
ETYPMNWPIGMGQSFFGIIDRKTKTIEPFRDEENVLHLNEDYELQESHAITSDSAYEQAIEELMLVDEAGETFDKEKLMT
GDLTPVFFGSALANFGVQNFLNAYVDHAPMPSGRKTESGEEISPFDESFSGFIFKIQANMNPQHRDRIAFMRIVSGAFER
GMDIKMTRTDKKMKISRSTSFMADDTQTVNHAVSGDIIGLYDSGNFQIGDTLVGGNQKFQFEKLPQFTPEIFMKVSPKNV
MKQKHFHKGIEQLVQEGAIQLYRTLHTNQIILGAVGQLQFEVFEHRMNNEYNVDVIMEPVGRKIARWIENEADIRDAMNS
SRSILVEDRFENKVFLFENEFATRWFLDKFPEIKLYSLL

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily

Homologues:

Organism=Homo sapiens, GI18390331, Length=486, Percent_Identity=30.2469135802469, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI19923640, Length=525, Percent_Identity=25.5238095238095, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI25306283, Length=390, Percent_Identity=28.974358974359, Blast_Score=154, Evalue=3e-37,
Organism=Homo sapiens, GI25306287, Length=300, Percent_Identity=31, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI157426893, Length=164, Percent_Identity=32.3170731707317, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI94966754, Length=138, Percent_Identity=31.1594202898551, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=28.1879194630872, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI310132016, Length=113, Percent_Identity=32.7433628318584, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI310110807, Length=113, Percent_Identity=32.7433628318584, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI310123363, Length=113, Percent_Identity=32.7433628318584, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI84043963, Length=135, Percent_Identity=32.5925925925926, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1790835, Length=527, Percent_Identity=45.9203036053131, Blast_Score=476, Evalue=1e-135,
Organism=Escherichia coli, GI1789738, Length=480, Percent_Identity=28.75, Blast_Score=185, Evalue=7e-48,
Organism=Escherichia coli, GI48994988, Length=180, Percent_Identity=32.7777777777778, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788922, Length=158, Percent_Identity=29.746835443038, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1789737, Length=133, Percent_Identity=30.8270676691729, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790412, Length=133, Percent_Identity=30.8270676691729, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17533571, Length=481, Percent_Identity=28.4823284823285, Blast_Score=188, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI17556745, Length=177, Percent_Identity=33.8983050847458, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17557151, Length=137, Percent_Identity=31.3868613138686, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=29.1925465838509, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71988819, Length=137, Percent_Identity=30.6569343065693, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71988811, Length=137, Percent_Identity=30.6569343065693, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=476, Percent_Identity=27.5210084033613, Blast_Score=189, Evalue=6e-49,
Organism=Saccharomyces cerevisiae, GI6322359, Length=521, Percent_Identity=27.447216890595, Blast_Score=154, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6323320, Length=138, Percent_Identity=29.7101449275362, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324707, Length=215, Percent_Identity=26.046511627907, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6320593, Length=215, Percent_Identity=26.046511627907, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24582462, Length=484, Percent_Identity=30.5785123966942, Blast_Score=207, Evalue=2e-53,
Organism=Drosophila melanogaster, GI221458488, Length=502, Percent_Identity=24.9003984063745, Blast_Score=159, Evalue=6e-39,
Organism=Drosophila melanogaster, GI78706572, Length=190, Percent_Identity=30, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=28.7581699346405, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24585711, Length=154, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24585713, Length=154, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF3_MACCJ (B9EAS8)

Other databases:

- EMBL:   AP009484
- RefSeq:   YP_002560035.1
- GeneID:   7390717
- GenomeReviews:   AP009484_GR
- KEGG:   mcl:MCCL_0632
- OMA:   LQFEVVQ
- ProtClustDB:   PRK00741
- GO:   GO:0005737
- HAMAP:   MF_00072
- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00503
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 59522; Mature: 59390

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLREEVEIRKTFAIISHPDAGKTTLTEKLLLFGGAIREAGTVKGKKTGKFATSDWMEVE
CCCHHHHHHHHHEEEEECCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHH
KQRGISVTSSVMQFDYDNFKINILDTPGHEDFSEDTYRTLMAVDSAVMVIDCAKGIEPQT
HHCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHH
LKLFKVCKMRGIPIFTFINKLDRVGKEPFELLEEIEKTLEIETYPMNWPIGMGQSFFGII
HHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHH
DRKTKTIEPFRDEENVLHLNEDYELQESHAITSDSAYEQAIEELMLVDEAGETFDKEKLM
CCCCCCCCCCCCCCCEEEECCCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHCC
TGDLTPVFFGSALANFGVQNFLNAYVDHAPMPSGRKTESGEEISPFDESFSGFIFKIQAN
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEC
MNPQHRDRIAFMRIVSGAFERGMDIKMTRTDKKMKISRSTSFMADDTQTVNHAVSGDIIG
CCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCCCCCHHHHHHHCCCCEEE
LYDSGNFQIGDTLVGGNQKFQFEKLPQFTPEIFMKVSPKNVMKQKHFHKGIEQLVQEGAI
EEECCCEEECCEEECCCCCEEHHHCCCCCCHHEEEECCHHHHHHHHHHHHHHHHHHHHHH
QLYRTLHTNQIILGAVGQLQFEVFEHRMNNEYNVDVIMEPVGRKIARWIENEADIRDAMN
HHHHHHCCCEEEEEHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHCCHHHHHHHHC
SSRSILVEDRFENKVFLFENEFATRWFLDKFPEIKLYSLL
CCCEEEEEECCCCEEEEEECCCHHHHHHHCCCCEEEEECC
>Mature Secondary Structure 
TLREEVEIRKTFAIISHPDAGKTTLTEKLLLFGGAIREAGTVKGKKTGKFATSDWMEVE
CCHHHHHHHHHEEEEECCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHH
KQRGISVTSSVMQFDYDNFKINILDTPGHEDFSEDTYRTLMAVDSAVMVIDCAKGIEPQT
HHCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHH
LKLFKVCKMRGIPIFTFINKLDRVGKEPFELLEEIEKTLEIETYPMNWPIGMGQSFFGII
HHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHH
DRKTKTIEPFRDEENVLHLNEDYELQESHAITSDSAYEQAIEELMLVDEAGETFDKEKLM
CCCCCCCCCCCCCCCEEEECCCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHCC
TGDLTPVFFGSALANFGVQNFLNAYVDHAPMPSGRKTESGEEISPFDESFSGFIFKIQAN
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEC
MNPQHRDRIAFMRIVSGAFERGMDIKMTRTDKKMKISRSTSFMADDTQTVNHAVSGDIIG
CCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCCCCCHHHHHHHCCCCEEE
LYDSGNFQIGDTLVGGNQKFQFEKLPQFTPEIFMKVSPKNVMKQKHFHKGIEQLVQEGAI
EEECCCEEECCEEECCCCCEEHHHCCCCCCHHEEEECCHHHHHHHHHHHHHHHHHHHHHH
QLYRTLHTNQIILGAVGQLQFEVFEHRMNNEYNVDVIMEPVGRKIARWIENEADIRDAMN
HHHHHHCCCEEEEEHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHCCHHHHHHHHC
SSRSILVEDRFENKVFLFENEFATRWFLDKFPEIKLYSLL
CCCEEEEEECCCCEEEEEECCCHHHHHHHCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA