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The map label for this gene is nifU [H]

Identifier: 222150795

GI number: 222150795

Start: 583771

End: 584235

Strand: Direct

Name: nifU [H]

Synonym: MCCL_0545

Alternate gene names: 222150795

Gene position: 583771-584235 (Clockwise)

Preceding gene: 222150794

Following gene: 222150796

Centisome position: 27.77

GC content: 38.06

Gene sequence:

>465_bases
ATGAGTTTTAATAATTTGGATCAGCTATATCGTTCAGTCATCATGGATCATTATAAGAACCCTCGTAATAAAGGGATTAT
TGAAGATGGGACATTAACGATTGATATGAACAACCCGACTTGTGGCGATAGAATTCGTTTAACTTTGGATGTAGTTGATG
ATAATGTTAAAGATGTAAAGTTTGAAGGTGAAGGATGTTCTATCTCGATGTCAAGTGCATCGATGATGACAGAAGCGATT
AAAGGGAAATCAGTAAGTGAAGCACTTGAAATGGGAGAAGAATTCTCTAAGATGATGCTTGGAGAAGACTATACATTTGA
TGAAAGTGCTGGAGATATCGAAGCGCTTTCTGGTGTGGCGAAGTTCCCTGCACGTATTAAATGTGCGACACTTGCCTGGA
AAGCTTTGGAACGTGGCACGAAAGAAAACAATGATACACATGTCAGTGAAGAAATAGATGAATAA

Upstream 100 bases:

>100_bases
CAGTCATCCACTGCACGTGCAAGTTTCTACATCTATAACACAAAAGAAGAAATCGATCAGCTAGTAACAAGCTTAAAAGA
AACGAAGGAGTTTTTCAGTT

Downstream 100 bases:

>100_bases
AATAAAAGGAGTGACGTCAATTGGCTAAAAAAGCACCTGAAGTTGGAGATTACAAATATGGTTTCCACGATAAAGACGTA
TCGATTTTCCGTTCAGAAAG

Product: nitrogen fixation protein NifU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MSFNNLDQLYRSVIMDHYKNPRNKGIIEDGTLTIDMNNPTCGDRIRLTLDVVDDNVKDVKFEGEGCSISMSSASMMTEAI
KGKSVSEALEMGEEFSKMMLGEDYTFDESAGDIEALSGVAKFPARIKCATLAWKALERGTKENNDTHVSEEIDE

Sequences:

>Translated_154_residues
MSFNNLDQLYRSVIMDHYKNPRNKGIIEDGTLTIDMNNPTCGDRIRLTLDVVDDNVKDVKFEGEGCSISMSSASMMTEAI
KGKSVSEALEMGEEFSKMMLGEDYTFDESAGDIEALSGVAKFPARIKCATLAWKALERGTKENNDTHVSEEIDE
>Mature_153_residues
SFNNLDQLYRSVIMDHYKNPRNKGIIEDGTLTIDMNNPTCGDRIRLTLDVVDDNVKDVKFEGEGCSISMSSASMMTEAIK
GKSVSEALEMGEEFSKMMLGEDYTFDESAGDIEALSGVAKFPARIKCATLAWKALERGTKENNDTHVSEEIDE

Specific function: May be involved in the formation or repair of [Fe-S] clusters present in iron-sulfur proteins (Potential) [H]

COG id: COG0822

COG function: function code C; NifU homolog involved in Fe-S cluster formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifU family [H]

Homologues:

Organism=Homo sapiens, GI56699456, Length=88, Percent_Identity=35.2272727272727, Blast_Score=65, Evalue=3e-11,
Organism=Escherichia coli, GI1788878, Length=83, Percent_Identity=36.144578313253, Blast_Score=63, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324800, Length=89, Percent_Identity=35.9550561797753, Blast_Score=66, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21355597, Length=85, Percent_Identity=40, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002871
- InterPro:   IPR011341 [H]

Pfam domain/function: PF01592 NifU_N [H]

EC number: NA

Molecular weight: Translated: 17101; Mature: 16970

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFNNLDQLYRSVIMDHYKNPRNKGIIEDGTLTIDMNNPTCGDRIRLTLDVVDDNVKDVK
CCCCHHHHHHHHHHHHHHCCCCCCCCEECCEEEEECCCCCCCCEEEEEEEEECCCCEEEE
FEGEGCSISMSSASMMTEAIKGKSVSEALEMGEEFSKMMLGEDYTFDESAGDIEALSGVA
ECCCCCEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
KFPARIKCATLAWKALERGTKENNDTHVSEEIDE
HCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHCCC
>Mature Secondary Structure 
SFNNLDQLYRSVIMDHYKNPRNKGIIEDGTLTIDMNNPTCGDRIRLTLDVVDDNVKDVK
CCCHHHHHHHHHHHHHHCCCCCCCCEECCEEEEECCCCCCCCEEEEEEEEECCCCEEEE
FEGEGCSISMSSASMMTEAIKGKSVSEALEMGEEFSKMMLGEDYTFDESAGDIEALSGVA
ECCCCCEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
KFPARIKCATLAWKALERGTKENNDTHVSEEIDE
HCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]