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The map label for this gene is nreB [H]

Identifier: 222150391

GI number: 222150391

Start: 146952

End: 147983

Strand: Direct

Name: nreB [H]

Synonym: MCCL_0141

Alternate gene names: 222150391

Gene position: 146952-147983 (Clockwise)

Preceding gene: 222150390

Following gene: 222150392

Centisome position: 6.99

GC content: 35.56

Gene sequence:

>1032_bases
GTGTCGAAGCTTGAGCATGAAGTAAATGATGATGGATTGGTTGATGCCTTTTATAACGATACCGATGAACTCATCATATT
TATAGATGAAAAGAACAATATACTCACAATGAATCAAGCCGCTCAAAAAGTGATTGATTTACATGATAATATTGATGGTC
TGACAAGAAATCTTTGTCGCACATGTTTAGGAGTTACAAGTGAAAATGCAATAATGGAGTGCAGGGACTGTTTTATTAAA
CGTGAACAAAATAATCAGTCGTTTCAATTATTTCTAAAAGATGAAAATGGGGAACCTAAACCGTATTCAGCATCTTACGT
CGTGCTAAAAGAAAATAACGGCACTAAAGTACTGACATTGCAAAATATTTCCCAGCAAATAAAAACGCAGGAAATTCTTC
ATCAGAAAACAATTACACAAAAAATTATTAGTGCGCAGGAAAATGAAAGAAAAAGAATCTCACGAGAATTGCATGATAGT
GTGATTCAAGAGTTGCTTAATGTATCTGTTGACATTCGTTTGATGAAATATAAAACTGAAGAAGAGAAAGAGAAGCATCT
TCAGATGATGGAAGGTTCAATCGCACGACTAATGGATGATATACGCAATCTATCCTTAGAGTTGAGGCCTTCTTCTTTGG
ATGATCTGGGTTTAGTAGCAGCATTTAACTCTCATTTTAAGCAACTTGAAAAGAGCTATGGCCTTGAAGTTAATATGGAT
GAGGATATAGGCAACATTCGTTTTTCTAATGAGATAGAAACAGCAGTATACAGAATTACACAAGAAGCAATACTAAATGC
CTTAAAATATGCGAATGTCGATGAAGTAAATGTATTTATACAAAAAGATGAAACTGAATTAACAGTAGAAATTAGCGACA
GTGGAGATGGGTTTACACCAGGAGATACACCAAAAGGATCTGGACTCGGATTCTTTGGTATGCAAGAACGTGCGGCTATC
GTCAATGGGCATGTTGAGATTAATAGCGAAACAGGAAAAGGTACGTTTGTAACGTTAACCATCCCACTCTAG

Upstream 100 bases:

>100_bases
AATAGAAGTAAACGTCCGTTTACTTTAACGATGCATGAACAGCTCAAAAAAAATCTTGAGCAAGGACTCGGTGCTTTTTA
TTGTAAGGATGTGATCAATA

Downstream 100 bases:

>100_bases
GGGGATAAATATGAAGATTGTAATTGCGGATGATCATGCAGTTGTGCGTACGGGATTCTCGATGATTTTGAACTATCAAG
ACAATATGGAAGTTGTTGCA

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: Nitrogen regulation protein B [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MSKLEHEVNDDGLVDAFYNDTDELIIFIDEKNNILTMNQAAQKVIDLHDNIDGLTRNLCRTCLGVTSENAIMECRDCFIK
REQNNQSFQLFLKDENGEPKPYSASYVVLKENNGTKVLTLQNISQQIKTQEILHQKTITQKIISAQENERKRISRELHDS
VIQELLNVSVDIRLMKYKTEEEKEKHLQMMEGSIARLMDDIRNLSLELRPSSLDDLGLVAAFNSHFKQLEKSYGLEVNMD
EDIGNIRFSNEIETAVYRITQEAILNALKYANVDEVNVFIQKDETELTVEISDSGDGFTPGDTPKGSGLGFFGMQERAAI
VNGHVEINSETGKGTFVTLTIPL

Sequences:

>Translated_343_residues
MSKLEHEVNDDGLVDAFYNDTDELIIFIDEKNNILTMNQAAQKVIDLHDNIDGLTRNLCRTCLGVTSENAIMECRDCFIK
REQNNQSFQLFLKDENGEPKPYSASYVVLKENNGTKVLTLQNISQQIKTQEILHQKTITQKIISAQENERKRISRELHDS
VIQELLNVSVDIRLMKYKTEEEKEKHLQMMEGSIARLMDDIRNLSLELRPSSLDDLGLVAAFNSHFKQLEKSYGLEVNMD
EDIGNIRFSNEIETAVYRITQEAILNALKYANVDEVNVFIQKDETELTVEISDSGDGFTPGDTPKGSGLGFFGMQERAAI
VNGHVEINSETGKGTFVTLTIPL
>Mature_342_residues
SKLEHEVNDDGLVDAFYNDTDELIIFIDEKNNILTMNQAAQKVIDLHDNIDGLTRNLCRTCLGVTSENAIMECRDCFIKR
EQNNQSFQLFLKDENGEPKPYSASYVVLKENNGTKVLTLQNISQQIKTQEILHQKTITQKIISAQENERKRISRELHDSV
IQELLNVSVDIRLMKYKTEEEKEKHLQMMEGSIARLMDDIRNLSLELRPSSLDDLGLVAAFNSHFKQLEKSYGLEVNMDE
DIGNIRFSNEIETAVYRITQEAILNALKYANVDEVNVFIQKDETELTVEISDSGDGFTPGDTPKGSGLGFFGMQERAAIV
NGHVEINSETGKGTFVTLTIPL

Specific function: Member of the two-component regulatory system nreB/nreC involved in the control of dissimilatory nitrate/nitrite reduction in response to oxygen. NreB functions as a direct oxygen sensor histidine kinase which is autophosphorylated, in the absence of oxyg

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788812, Length=197, Percent_Identity=27.9187817258883, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787474, Length=217, Percent_Identity=23.963133640553, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87082311, Length=232, Percent_Identity=25, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017203 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 38855; Mature: 38724

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLEHEVNDDGLVDAFYNDTDELIIFIDEKNNILTMNQAAQKVIDLHDNIDGLTRNLCR
CCCCCCCCCCCCEEEHEECCCCEEEEEEECCCCEEEHHHHHHHHHHHHCCHHHHHHHHHH
TCLGVTSENAIMECRDCFIKREQNNQSFQLFLKDENGEPKPYSASYVVLKENNGTKVLTL
HHHCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCEEEEEECCCCCEEEEE
QNISQQIKTQEILHQKTITQKIISAQENERKRISRELHDSVIQELLNVSVDIRLMKYKTE
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCH
EEKEKHLQMMEGSIARLMDDIRNLSLELRPSSLDDLGLVAAFNSHFKQLEKSYGLEVNMD
HHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEC
EDIGNIRFSNEIETAVYRITQEAILNALKYANVDEVNVFIQKDETELTVEISDSGDGFTP
CCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEEEEEEECCCCCCCC
GDTPKGSGLGFFGMQERAAIVNGHVEINSETGKGTFVTLTIPL
CCCCCCCCCEECCCCCCCEEEECEEEEECCCCCEEEEEEEECC
>Mature Secondary Structure 
SKLEHEVNDDGLVDAFYNDTDELIIFIDEKNNILTMNQAAQKVIDLHDNIDGLTRNLCR
CCCCCCCCCCCEEEHEECCCCEEEEEEECCCCEEEHHHHHHHHHHHHCCHHHHHHHHHH
TCLGVTSENAIMECRDCFIKREQNNQSFQLFLKDENGEPKPYSASYVVLKENNGTKVLTL
HHHCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCEEEEEECCCCCEEEEE
QNISQQIKTQEILHQKTITQKIISAQENERKRISRELHDSVIQELLNVSVDIRLMKYKTE
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCH
EEKEKHLQMMEGSIARLMDDIRNLSLELRPSSLDDLGLVAAFNSHFKQLEKSYGLEVNMD
HHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEC
EDIGNIRFSNEIETAVYRITQEAILNALKYANVDEVNVFIQKDETELTVEISDSGDGFTP
CCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEEEEEEECCCCCCCC
GDTPKGSGLGFFGMQERAAIVNGHVEINSETGKGTFVTLTIPL
CCCCCCCCCEECCCCCCCEEEECEEEEECCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA