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The map label for this gene is nreC [H]

Identifier: 222150392

GI number: 222150392

Start: 147994

End: 148644

Strand: Direct

Name: nreC [H]

Synonym: MCCL_0142

Alternate gene names: 222150392

Gene position: 147994-148644 (Clockwise)

Preceding gene: 222150391

Following gene: 222150393

Centisome position: 7.04

GC content: 35.18

Gene sequence:

>651_bases
ATGAAGATTGTAATTGCGGATGATCATGCAGTTGTGCGTACGGGATTCTCGATGATTTTGAACTATCAAGACAATATGGA
AGTTGTTGCAACAGCAGCAGATGGTATGGAAGCTTTCCAAATGGTCTCGCAATATAAACCAGATGTACTGATTATGGACT
TAAGTATGCCGCCAGGAGAATCAGGATTAATTGCTACAGGTAAAATAAAAGAGGCATTTCCTGAGACAAAGATACTTATA
TTGACGATGTATGATGATGAAGAGTATTTGTTTCATGTTCTCAAAAATGGTGCAAACGGCTATATTCTGAAAAATGCTCC
AGACGAAGAGTTGATTAAAGCAGTGCGAACGGTTTATCAAGAAGGAACTTATATCGATCCTAAGATGGCAACGTCACTCG
TGCATGAATTAATACACCATGATACAGAATACTCAGCGAAAAATGATCATTTGAAAATATTAACAAAACGAGAATTAGAG
ATATTACCTTTAATTGCGAAAGGCTATGGTAATAAAGAAATAGCTGAAATGTTATATGTTTCAGTAAAAACTGTAGAAGC
ACATAAAGCAAGAATCATGGATAAGCTAGAGCTTAAGAGTAAACCAGAACTTGTAGAATATGCCTTAAAGAAAAAACTGC
TAGAATTTTAA

Upstream 100 bases:

>100_bases
CAAGAACGTGCGGCTATCGTCAATGGGCATGTTGAGATTAATAGCGAAACAGGAAAAGGTACGTTTGTAACGTTAACCAT
CCCACTCTAGGGGGATAAAT

Downstream 100 bases:

>100_bases
AAAAGGTAGTGACACGAGCATTCAGACACAAATAAAATCATGAAAAAGCGCAAAAGCAAGTGTTTTTGCTGCGAGCGCTT
TTTTGATTTTACGAAGTGTC

Product: two-component response regulator NreC

Products: NA

Alternate protein names: Nitrogen regulation protein C [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MKIVIADDHAVVRTGFSMILNYQDNMEVVATAADGMEAFQMVSQYKPDVLIMDLSMPPGESGLIATGKIKEAFPETKILI
LTMYDDEEYLFHVLKNGANGYILKNAPDEELIKAVRTVYQEGTYIDPKMATSLVHELIHHDTEYSAKNDHLKILTKRELE
ILPLIAKGYGNKEIAEMLYVSVKTVEAHKARIMDKLELKSKPELVEYALKKKLLEF

Sequences:

>Translated_216_residues
MKIVIADDHAVVRTGFSMILNYQDNMEVVATAADGMEAFQMVSQYKPDVLIMDLSMPPGESGLIATGKIKEAFPETKILI
LTMYDDEEYLFHVLKNGANGYILKNAPDEELIKAVRTVYQEGTYIDPKMATSLVHELIHHDTEYSAKNDHLKILTKRELE
ILPLIAKGYGNKEIAEMLYVSVKTVEAHKARIMDKLELKSKPELVEYALKKKLLEF
>Mature_216_residues
MKIVIADDHAVVRTGFSMILNYQDNMEVVATAADGMEAFQMVSQYKPDVLIMDLSMPPGESGLIATGKIKEAFPETKILI
LTMYDDEEYLFHVLKNGANGYILKNAPDEELIKAVRTVYQEGTYIDPKMATSLVHELIHHDTEYSAKNDHLKILTKRELE
ILPLIAKGYGNKEIAEMLYVSVKTVEAHKARIMDKLELKSKPELVEYALKKKLLEF

Specific function: Member of the two-component regulatory system nreB/nreC involved in the control of dissimilatory nitrate/nitrite reduction in response to oxygen. Phosphorylated nreC binds to a GC-rich palindromic sequence at the promoters of the nitrate (narGHJI) and nit

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=214, Percent_Identity=32.7102803738318, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1788521, Length=200, Percent_Identity=32, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1790102, Length=206, Percent_Identity=32.5242718446602, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1786747, Length=206, Percent_Identity=28.1553398058252, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1788712, Length=213, Percent_Identity=28.6384976525822, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1788546, Length=209, Percent_Identity=31.1004784688995, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI1787473, Length=186, Percent_Identity=30.6451612903226, Blast_Score=80, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 24478; Mature: 24478

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVIADDHAVVRTGFSMILNYQDNMEVVATAADGMEAFQMVSQYKPDVLIMDLSMPPGE
CEEEEECCCHHHHHHHHHEEECCCCCEEEEEEHHHHHHHHHHHHCCCCEEEEEECCCCCC
SGLIATGKIKEAFPETKILILTMYDDEEYLFHVLKNGANGYILKNAPDEELIKAVRTVYQ
CCEEEECCHHHHCCCCEEEEEEEECCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHH
EGTYIDPKMATSLVHELIHHDTEYSAKNDHLKILTKRELEILPLIAKGYGNKEIAEMLYV
CCCEECHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHH
SVKTVEAHKARIMDKLELKSKPELVEYALKKKLLEF
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIVIADDHAVVRTGFSMILNYQDNMEVVATAADGMEAFQMVSQYKPDVLIMDLSMPPGE
CEEEEECCCHHHHHHHHHEEECCCCCEEEEEEHHHHHHHHHHHHCCCCEEEEEECCCCCC
SGLIATGKIKEAFPETKILILTMYDDEEYLFHVLKNGANGYILKNAPDEELIKAVRTVYQ
CCEEEECCHHHHCCCCEEEEEEEECCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHH
EGTYIDPKMATSLVHELIHHDTEYSAKNDHLKILTKRELEILPLIAKGYGNKEIAEMLYV
CCCEECHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHH
SVKTVEAHKARIMDKLELKSKPELVEYALKKKLLEF
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12426351 [H]