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The map label for this gene is purA [H]

Identifier: 222150269

GI number: 222150269

Start: 23502

End: 24791

Strand: Direct

Name: purA [H]

Synonym: MCCL_0019

Alternate gene names: 222150269

Gene position: 23502-24791 (Clockwise)

Preceding gene: 222150268

Following gene: 222150271

Centisome position: 1.12

GC content: 36.51

Gene sequence:

>1290_bases
ATGTCATCAATCGTAGTTGTTGGGACTCAATGGGGAGACGAAGGTAAAGGTAAAATCACGGACTTTTTAGCAGAAGAAGC
AAACGTTATTGCGCGTTTTTCAGGTGGCAATAACGCAGGACATACAATCAAATTTGATGGTGAAACATACAAATTGCATT
TAGTACCATCAGGTATTTTCTATTCAGATAAGCTATCTGTTATTGGAAATGGTGTAGTAATAGATCCTGTTGCATTATTA
AAAGAATTAGATGCCCTAAATGCACGTGGTGTGAAAACTGATAACCTACGCATTTCAAACCGTGCACAAGTTATTTTGCC
GTATCACTTAAAACAAGATGAATTAGAAGAAGAAAAACGTGGTGATAATAAGATTGGTACGACGAAAAAAGGAATCGGAC
CAGCTTATGTAGATAAAGTACAACGTATCGGTATTCGTATGGCGGATTTACTTGATAAAGAAACGTTTGAAACAAAGTTA
AAAGAAAATGTAGCAATTAAAAACGAAATGTTTGAGAAATTATTTGATGCTGAAGGTTTCAAATTTGAAGATATTTTTGA
TGTATATTATGCAGCTGGACAACGATTAAAAGAATATACTGTAGATACTGCAAAAGTATTAGACGATGCATTTATGGCAG
ATGAAAAAGTGTTATTTGAAGGTGCTCAAGGAGTAATGCTTGATATCGATCATGGTACTTATCCGTTCGTCACTTCAAGT
AACCCGATTGCTGGTAACGTAACAGTTGGTACTGGTGTAGGTCCAACGTTTGTAGATAAAGTTGTTGGTGTATGTAAAGC
TTACACATCACGTGTAGGGGATGGACCATTCCCAACTGAATTATTTGATGAAGATGGTCATCATATTCGTGAAGTAGGTC
GTGAATATGGAACGACTACAGGGCGTCCACGTCGTGTAGGTTGGTTTGACTCTGTTGTATTACGTCATTCTCGCCGTGCA
AGTGGTATTACGGACTTGTCAATTAACTCTATTGACGTATTAACTGGTTTAGAAACTGTTAAGATTTGTACAGCATATGA
ATTGGATGGAAAAGAGATTACAGAATATCCAGCGAATTTAAATGAACTAAATCGTTGTAAACCGATTTTCGAAGAATTAC
CAGGATGGACTGAAGATGTGACTTCATGTAAGTCATTAGACGAGCTACCTGATAACGCACGCCGTTACTTAGAGCGTATT
TCTGAGTTATGTAATGTTAAGATTTCTATCTTCTCAGTTGGACCAGATAGAAATCAAACGAACTTACTTGAAAACTTATG
GGAAAAATAA

Upstream 100 bases:

>100_bases
AAATAAGTGAATATTTATTAATGTTCGTTTTTCGGTTGATTTTTCCCGATTTCATTGTTAAAATGTTTAAGGTTAGTGAA
CAAAATTTGGAGGTGCACTC

Downstream 100 bases:

>100_bases
GAGGCTATCATCTGAGCGTTTAGCTTTAACCTAAAATAAAAGCCATAAAAACATTTAATGTTTTTATGGCTTTTAAAATT
GCTTTAATGTATATCTTTCT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSSIVVVGTQWGDEGKGKITDFLAEEANVIARFSGGNNAGHTIKFDGETYKLHLVPSGIFYSDKLSVIGNGVVIDPVALL
KELDALNARGVKTDNLRISNRAQVILPYHLKQDELEEEKRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLDKETFETKL
KENVAIKNEMFEKLFDAEGFKFEDIFDVYYAAGQRLKEYTVDTAKVLDDAFMADEKVLFEGAQGVMLDIDHGTYPFVTSS
NPIAGNVTVGTGVGPTFVDKVVGVCKAYTSRVGDGPFPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRA
SGITDLSINSIDVLTGLETVKICTAYELDGKEITEYPANLNELNRCKPIFEELPGWTEDVTSCKSLDELPDNARRYLERI
SELCNVKISIFSVGPDRNQTNLLENLWEK

Sequences:

>Translated_429_residues
MSSIVVVGTQWGDEGKGKITDFLAEEANVIARFSGGNNAGHTIKFDGETYKLHLVPSGIFYSDKLSVIGNGVVIDPVALL
KELDALNARGVKTDNLRISNRAQVILPYHLKQDELEEEKRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLDKETFETKL
KENVAIKNEMFEKLFDAEGFKFEDIFDVYYAAGQRLKEYTVDTAKVLDDAFMADEKVLFEGAQGVMLDIDHGTYPFVTSS
NPIAGNVTVGTGVGPTFVDKVVGVCKAYTSRVGDGPFPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRA
SGITDLSINSIDVLTGLETVKICTAYELDGKEITEYPANLNELNRCKPIFEELPGWTEDVTSCKSLDELPDNARRYLERI
SELCNVKISIFSVGPDRNQTNLLENLWEK
>Mature_428_residues
SSIVVVGTQWGDEGKGKITDFLAEEANVIARFSGGNNAGHTIKFDGETYKLHLVPSGIFYSDKLSVIGNGVVIDPVALLK
ELDALNARGVKTDNLRISNRAQVILPYHLKQDELEEEKRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLDKETFETKLK
ENVAIKNEMFEKLFDAEGFKFEDIFDVYYAAGQRLKEYTVDTAKVLDDAFMADEKVLFEGAQGVMLDIDHGTYPFVTSSN
PIAGNVTVGTGVGPTFVDKVVGVCKAYTSRVGDGPFPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRAS
GITDLSINSIDVLTGLETVKICTAYELDGKEITEYPANLNELNRCKPIFEELPGWTEDVTSCKSLDELPDNARRYLERIS
ELCNVKISIFSVGPDRNQTNLLENLWEK

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=422, Percent_Identity=41.7061611374408, Blast_Score=315, Evalue=7e-86,
Organism=Homo sapiens, GI34577063, Length=422, Percent_Identity=39.3364928909953, Blast_Score=305, Evalue=7e-83,
Organism=Homo sapiens, GI40316944, Length=392, Percent_Identity=40.3061224489796, Blast_Score=274, Evalue=1e-73,
Organism=Escherichia coli, GI1790620, Length=426, Percent_Identity=47.4178403755869, Blast_Score=410, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI25146553, Length=421, Percent_Identity=40.1425178147268, Blast_Score=306, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI25146555, Length=421, Percent_Identity=40.1425178147268, Blast_Score=305, Evalue=2e-83,
Organism=Saccharomyces cerevisiae, GI6324109, Length=424, Percent_Identity=41.0377358490566, Blast_Score=330, Evalue=3e-91,
Organism=Drosophila melanogaster, GI21356033, Length=420, Percent_Identity=39.2857142857143, Blast_Score=303, Evalue=1e-82,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 47691; Mature: 47560

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00178 AA_TRNA_LIGASE_I ; PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIVVVGTQWGDEGKGKITDFLAEEANVIARFSGGNNAGHTIKFDGETYKLHLVPSGIF
CCCEEEEEECCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCEEEECCCEEEEEEEECCEE
YSDKLSVIGNGVVIDPVALLKELDALNARGVKTDNLRISNRAQVILPYHLKQDELEEEKR
ECCCHHHCCCCEEECHHHHHHHHHHCCCCCCCCCCEEECCCCEEEEEECCCHHHHHHHHC
GDNKIGTTKKGIGPAYVDKVQRIGIRMADLLDKETFETKLKENVAIKNEMFEKLFDAEGF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KFEDIFDVYYAAGQRLKEYTVDTAKVLDDAFMADEKVLFEGAQGVMLDIDHGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCCEEEEEECCCCCCEEECC
NPIAGNVTVGTGVGPTFVDKVVGVCKAYTSRVGDGPFPTELFDEDGHHIREVGREYGTTT
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHCCCC
GRPRRVGWFDSVVLRHSRRASGITDLSINSIDVLTGLETVKICTAYELDGKEITEYPANL
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCEEEEEEEECCCCHHHHCCCCH
NELNRCKPIFEELPGWTEDVTSCKSLDELPDNARRYLERISELCNVKISIFSVGPDRNQT
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEEECCCCCHH
NLLENLWEK
HHHHHHHCC
>Mature Secondary Structure 
SSIVVVGTQWGDEGKGKITDFLAEEANVIARFSGGNNAGHTIKFDGETYKLHLVPSGIF
CCEEEEEECCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCEEEECCCEEEEEEEECCEE
YSDKLSVIGNGVVIDPVALLKELDALNARGVKTDNLRISNRAQVILPYHLKQDELEEEKR
ECCCHHHCCCCEEECHHHHHHHHHHCCCCCCCCCCEEECCCCEEEEEECCCHHHHHHHHC
GDNKIGTTKKGIGPAYVDKVQRIGIRMADLLDKETFETKLKENVAIKNEMFEKLFDAEGF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KFEDIFDVYYAAGQRLKEYTVDTAKVLDDAFMADEKVLFEGAQGVMLDIDHGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCCEEEEEECCCCCCEEECC
NPIAGNVTVGTGVGPTFVDKVVGVCKAYTSRVGDGPFPTELFDEDGHHIREVGREYGTTT
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHCCCC
GRPRRVGWFDSVVLRHSRRASGITDLSINSIDVLTGLETVKICTAYELDGKEITEYPANL
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCEEEEEEEECCCCHHHHCCCCH
NELNRCKPIFEELPGWTEDVTSCKSLDELPDNARRYLERISELCNVKISIFSVGPDRNQT
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEEECCCCCHH
NLLENLWEK
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA