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The map label for this gene is dnaB [H]

Identifier: 222150268

GI number: 222150268

Start: 21773

End: 23179

Strand: Direct

Name: dnaB [H]

Synonym: MCCL_0018

Alternate gene names: 222150268

Gene position: 21773-23179 (Clockwise)

Preceding gene: 222150267

Following gene: 222150269

Centisome position: 1.04

GC content: 39.09

Gene sequence:

>1407_bases
ATGGCAGATTATCTTGAAGGTAAGAAACTCCCGTTTAATTTAGTTGCGGAACAGTCAGTTTTAGGCGCTATCTTTATCGA
TAATGAAGTATTATCTTCCGTTCTAGAAGTACTCGTTCCTGGACATTTTTACAGACAGTCGCATCAACATATATTTGAAG
CGATGATGACATTGTCTGAGAAAAATGAAATAGTAGATATTGTAACTGTAGCGGATCAGATGACATCAGACGGGACATTA
GAAGCGGCTGGTGGAGAGATGTATCTTGTAGATTTAGCTGCAATTGTACCGAGTGCCCGTAATGTAGATTTTTACGCGGA
TATCGTCTATAAAGATGCGGTAAAGCGACGATTAATTCATACGGCAGACACTATTATGGCGGATGGTTATAATGAGACAC
TAGATCTCGAAACGTTACTTTCTGATGCCGAAAAGCGTATTATGGAAGTGTCTACTTCTAGAGGATCAGAAGGTTTTCAG
GAAATCAAAGATGTATTGACGCAAGTCTTTAATAATGCTGAAGAACTTGATAAGAACCAAGGGCAGACCCCAGGTATCCC
GACTGGATATAGAGATCTTGATCAAATGACGGCTGGATTCAATAGAAATGATCTGATCATTATTGCAGCACGTCCTTCGG
TAGGTAAGACCGCTTTCGCCTTAAATATTGCAACACAAGTAGCAACGCATGATCGAGGCGATGAGACGCCTTATAGTGTG
GGGATATTTAGTCTTGAGATGGGCGCTGATCAGTTAGCAACACGTATGATTTGTAGTACAGGTAACGTAGATTCTAATAG
ATTACGTACAGGAACAATGGATGGAGAAGACTGGAATCGTTTCACAGTAGCAGTGGGTAAGCTATCTAAGACAAATATAT
ATATAGATGATACACCAGGTATCCGTATTACAGATATCCGTTCTAAGTGTAGAAGACTAAAACAAGAACATGGATTAGAT
ATGATACTGATTGATTATCTTCAGCTTATTCAAGGTTCAGGCAGTCGCTCCAGTGACAATAGACAACAAGAAGTATCAGA
AATCTCGCGTATGCTGAAAGCTTTAGCGAGAGAACTAGAATGTCCTGTAATTGCGTTATCACAGTTATCTCGTGGCGTGG
AACAAAGACAGGATAAACGTCCAATGATGAGTGATATTCGTGAATCAGGTTCGATTGAGCAAGATGCAGATATCGTTGCA
TTCTTATATCGAGATGACTATTACAATCGTGGTAATGGTGAAGATGGTGAAGAAGAAGTAGATGCAGGTGCTCAAGAGGA
GAACGGAGAAATAGAAATTATTATTGCAAAGCAACGTAATGGTCCGACAGGAACAGTTAAACTTCATTTCTTAAAGCAAT
ATAATAAATTTACAGATATTGATTATGCACATTCAGATGTATATTAA

Upstream 100 bases:

>100_bases
GAAGTTTAGGCTATACAAATGTACCTGTTAAACTTCATAACAAGGTAATCGGTACATTAAAAGTGCATGTAACTGAAGCT
TAATAGATAAGGAGGATAAT

Downstream 100 bases:

>100_bases
TGAGTAAAGTGTATAAAACGAATAAAAACCGTACATTAATTTTTGTTTATAATTAATTGTACGGTTTTTATGTTGTATTT
AAAAGAAAAAACATAAGATA

Product: DnaB helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MADYLEGKKLPFNLVAEQSVLGAIFIDNEVLSSVLEVLVPGHFYRQSHQHIFEAMMTLSEKNEIVDIVTVADQMTSDGTL
EAAGGEMYLVDLAAIVPSARNVDFYADIVYKDAVKRRLIHTADTIMADGYNETLDLETLLSDAEKRIMEVSTSRGSEGFQ
EIKDVLTQVFNNAEELDKNQGQTPGIPTGYRDLDQMTAGFNRNDLIIIAARPSVGKTAFALNIATQVATHDRGDETPYSV
GIFSLEMGADQLATRMICSTGNVDSNRLRTGTMDGEDWNRFTVAVGKLSKTNIYIDDTPGIRITDIRSKCRRLKQEHGLD
MILIDYLQLIQGSGSRSSDNRQQEVSEISRMLKALARELECPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDADIVA
FLYRDDYYNRGNGEDGEEEVDAGAQEENGEIEIIIAKQRNGPTGTVKLHFLKQYNKFTDIDYAHSDVY

Sequences:

>Translated_468_residues
MADYLEGKKLPFNLVAEQSVLGAIFIDNEVLSSVLEVLVPGHFYRQSHQHIFEAMMTLSEKNEIVDIVTVADQMTSDGTL
EAAGGEMYLVDLAAIVPSARNVDFYADIVYKDAVKRRLIHTADTIMADGYNETLDLETLLSDAEKRIMEVSTSRGSEGFQ
EIKDVLTQVFNNAEELDKNQGQTPGIPTGYRDLDQMTAGFNRNDLIIIAARPSVGKTAFALNIATQVATHDRGDETPYSV
GIFSLEMGADQLATRMICSTGNVDSNRLRTGTMDGEDWNRFTVAVGKLSKTNIYIDDTPGIRITDIRSKCRRLKQEHGLD
MILIDYLQLIQGSGSRSSDNRQQEVSEISRMLKALARELECPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDADIVA
FLYRDDYYNRGNGEDGEEEVDAGAQEENGEIEIIIAKQRNGPTGTVKLHFLKQYNKFTDIDYAHSDVY
>Mature_467_residues
ADYLEGKKLPFNLVAEQSVLGAIFIDNEVLSSVLEVLVPGHFYRQSHQHIFEAMMTLSEKNEIVDIVTVADQMTSDGTLE
AAGGEMYLVDLAAIVPSARNVDFYADIVYKDAVKRRLIHTADTIMADGYNETLDLETLLSDAEKRIMEVSTSRGSEGFQE
IKDVLTQVFNNAEELDKNQGQTPGIPTGYRDLDQMTAGFNRNDLIIIAARPSVGKTAFALNIATQVATHDRGDETPYSVG
IFSLEMGADQLATRMICSTGNVDSNRLRTGTMDGEDWNRFTVAVGKLSKTNIYIDDTPGIRITDIRSKCRRLKQEHGLDM
ILIDYLQLIQGSGSRSSDNRQQEVSEISRMLKALARELECPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDADIVAF
LYRDDYYNRGNGEDGEEEVDAGAQEENGEIEIIIAKQRNGPTGTVKLHFLKQYNKFTDIDYAHSDVY

Specific function: Participates In Initiation And Elongation During Chromosome Replication; It Exhibits DNA-Dependent ATPase Activity And Contains Distinct Active Sites For ATP Binding, DNA Binding, And Interaction With DNAc Protein, Primase, And Other Prepriming Proteins.

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=458, Percent_Identity=44.9781659388646, Blast_Score=360, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 52212; Mature: 52081

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADYLEGKKLPFNLVAEQSVLGAIFIDNEVLSSVLEVLVPGHFYRQSHQHIFEAMMTLSE
CCCCCCCCCCCHHHHHHHHHHEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
KNEIVDIVTVADQMTSDGTLEAAGGEMYLVDLAAIVPSARNVDFYADIVYKDAVKRRLIH
CCCEEEEEEEHHHHCCCCCEEECCCCEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
TADTIMADGYNETLDLETLLSDAEKRIMEVSTSRGSEGFQEIKDVLTQVFNNAEELDKNQ
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHCC
GQTPGIPTGYRDLDQMTAGFNRNDLIIIAARPSVGKTAFALNIATQVATHDRGDETPYSV
CCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEEHHHHHHHCCCCCCCCCEE
GIFSLEMGADQLATRMICSTGNVDSNRLRTGTMDGEDWNRFTVAVGKLSKTNIYIDDTPG
EEEEEECCHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCEEEEEEECCEECEEEEECCCC
IRITDIRSKCRRLKQEHGLDMILIDYLQLIQGSGSRSSDNRQQEVSEISRMLKALARELE
EEEEHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
CPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDADIVAFLYRDDYYNRGNGEDGEEEV
CCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHH
DAGAQEENGEIEIIIAKQRNGPTGTVKLHFLKQYNKFTDIDYAHSDVY
CCCCCCCCCCEEEEEEECCCCCCCEEEEEEEHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ADYLEGKKLPFNLVAEQSVLGAIFIDNEVLSSVLEVLVPGHFYRQSHQHIFEAMMTLSE
CCCCCCCCCCHHHHHHHHHHEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
KNEIVDIVTVADQMTSDGTLEAAGGEMYLVDLAAIVPSARNVDFYADIVYKDAVKRRLIH
CCCEEEEEEEHHHHCCCCCEEECCCCEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
TADTIMADGYNETLDLETLLSDAEKRIMEVSTSRGSEGFQEIKDVLTQVFNNAEELDKNQ
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHCC
GQTPGIPTGYRDLDQMTAGFNRNDLIIIAARPSVGKTAFALNIATQVATHDRGDETPYSV
CCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEEHHHHHHHCCCCCCCCCEE
GIFSLEMGADQLATRMICSTGNVDSNRLRTGTMDGEDWNRFTVAVGKLSKTNIYIDDTPG
EEEEEECCHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCEEEEEEECCEECEEEEECCCC
IRITDIRSKCRRLKQEHGLDMILIDYLQLIQGSGSRSSDNRQQEVSEISRMLKALARELE
EEEEHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
CPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDADIVAFLYRDDYYNRGNGEDGEEEV
CCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHH
DAGAQEENGEIEIIIAKQRNGPTGTVKLHFLKQYNKFTDIDYAHSDVY
CCCCCCCCCCEEEEEEECCCCCCCEEEEEEEHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 1312531 [H]