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The map label for this gene is purD [H]

Identifier: 222110897

GI number: 222110897

Start: 1810233

End: 1811519

Strand: Direct

Name: purD [H]

Synonym: Dtpsy_1704

Alternate gene names: 222110897

Gene position: 1810233-1811519 (Clockwise)

Preceding gene: 222110896

Following gene: 222110898

Centisome position: 47.68

GC content: 67.06

Gene sequence:

>1287_bases
ATGAAAATCCTAGTCATTGGCGGCGGCGGCCGGGAACACGCACTGGCCTGGAAGCTGAGCCAGTCCCCCAAGACCTCCAA
GGTGTACGTGGCGCCCGGCAACGGCGGCACGGCGCTGTCGCCAAAGTATGAGAACGTGCCCATCAGCGACGTGACGGCCC
TGCGCGAGTGGGCGCAGGCGCAGAAGATCGCACTCACCGTGGTCGGCCCCGAGGCACCGCTGGCCGCTGGCGTGGTGGAC
GAGTTCCGTACCCACGGCCTGCGCATCTTCGGCCCCACGCAGGCTGCCGCGCAACTGGAAAGCTCCAAGGCCTTCTCCAA
GGCCTTCATGAAGCGCCACGGCATCCCCACGGCCGAGTACGAGACCTTCACCGACCCGCAGGCCGCGCATGCCTACGTGA
ACCGTCTGGGCGCACCCATCGTCATCAAGGCCGACGGCCTGGCGGCGGGCAAGGGCGTAGTGGTAGCCATGACGCTGCAG
GAGGCCCACGACGCCGTGGACTTCATGCTGGTGGACAACAAGTACGGCGTGGCCCACAACGAAGGCGGCGCCCGCGTGGT
GATCGAGCAGTTCCTCGAAGGTGAGGAAGCCTCGTTCATCGTGCTGTGCGACGGCAAGAACGTCCTGGCGCTGGCCACCA
GCCAGGACCACAAGCGCCTGAAGGATGGCGACCAAGGCCCCAACACCGGTGGCATGGGCGCGTATTCGCCTGCGCCGGTG
GTCACGGCCGATGTGCATGCGCGCGCCATGCGCGAGGTGATCCTACCCACCATCCGTGGCATGGAAAAAGACGGCATCCC
CTACACGGGCTTTCTGTATGCCGGCCTGATGATCGACGCGACGGGCCATCCCAAAACGTTGGAGTTCAACTGCCGCATGG
GCGACCCCGAGACACAGCCCATCCTCATGCGCCTCAAGAGCGACCTGGTGGAGGTGCTGGGGGCGGCCGTGGACGGCAAG
CTCGACCAGGTGGAACTGCAATGGGATCGCCGCACGGCGCTGGGCGTGGTGATGGCTGCCCAGGGCTATCCCGAGAACCC
GCGCAAGGGCGACGCCATCACCGGCCTGCCGCAGGACGCGGACGACGCCATGGTCTTCCACGCCGGCACACAGCTCGTGG
ACGGCGTGGTGCGGACCAGTGGCGGGCGCGTGCTGTGCGTGACGGCGCTGGCCGACAGCGTCAAGCAGGCACAGCAGCGC
GTGTATGACGTGGCGCGCGGCATTCACTTTGACGGCGCGCAGTACCGCCACGACATTGGCCACCGCGCGGTGAAGGCCAA
CGGATGA

Upstream 100 bases:

>100_bases
CGCGCGCATGCAAAAGCTCCTGGACATGATCGAAGACCTCGACGATGTGCAAGAGGTCTACCACAACGCAGAGCTCTAGG
AACTTGAGCACCGACACCCT

Downstream 100 bases:

>100_bases
CCACACAAAAACCCACCTTCAACACGGCGGACACCGTGGCCCGCGTGCGCGGCTATCTGGAGGGCCTGCAGGCGCGCATC
ACCAGCGCGCTGGAGGACAT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MKILVIGGGGREHALAWKLSQSPKTSKVYVAPGNGGTALSPKYENVPISDVTALREWAQAQKIALTVVGPEAPLAAGVVD
EFRTHGLRIFGPTQAAAQLESSKAFSKAFMKRHGIPTAEYETFTDPQAAHAYVNRLGAPIVIKADGLAAGKGVVVAMTLQ
EAHDAVDFMLVDNKYGVAHNEGGARVVIEQFLEGEEASFIVLCDGKNVLALATSQDHKRLKDGDQGPNTGGMGAYSPAPV
VTADVHARAMREVILPTIRGMEKDGIPYTGFLYAGLMIDATGHPKTLEFNCRMGDPETQPILMRLKSDLVEVLGAAVDGK
LDQVELQWDRRTALGVVMAAQGYPENPRKGDAITGLPQDADDAMVFHAGTQLVDGVVRTSGGRVLCVTALADSVKQAQQR
VYDVARGIHFDGAQYRHDIGHRAVKANG

Sequences:

>Translated_428_residues
MKILVIGGGGREHALAWKLSQSPKTSKVYVAPGNGGTALSPKYENVPISDVTALREWAQAQKIALTVVGPEAPLAAGVVD
EFRTHGLRIFGPTQAAAQLESSKAFSKAFMKRHGIPTAEYETFTDPQAAHAYVNRLGAPIVIKADGLAAGKGVVVAMTLQ
EAHDAVDFMLVDNKYGVAHNEGGARVVIEQFLEGEEASFIVLCDGKNVLALATSQDHKRLKDGDQGPNTGGMGAYSPAPV
VTADVHARAMREVILPTIRGMEKDGIPYTGFLYAGLMIDATGHPKTLEFNCRMGDPETQPILMRLKSDLVEVLGAAVDGK
LDQVELQWDRRTALGVVMAAQGYPENPRKGDAITGLPQDADDAMVFHAGTQLVDGVVRTSGGRVLCVTALADSVKQAQQR
VYDVARGIHFDGAQYRHDIGHRAVKANG
>Mature_428_residues
MKILVIGGGGREHALAWKLSQSPKTSKVYVAPGNGGTALSPKYENVPISDVTALREWAQAQKIALTVVGPEAPLAAGVVD
EFRTHGLRIFGPTQAAAQLESSKAFSKAFMKRHGIPTAEYETFTDPQAAHAYVNRLGAPIVIKADGLAAGKGVVVAMTLQ
EAHDAVDFMLVDNKYGVAHNEGGARVVIEQFLEGEEASFIVLCDGKNVLALATSQDHKRLKDGDQGPNTGGMGAYSPAPV
VTADVHARAMREVILPTIRGMEKDGIPYTGFLYAGLMIDATGHPKTLEFNCRMGDPETQPILMRLKSDLVEVLGAAVDGK
LDQVELQWDRRTALGVVMAAQGYPENPRKGDAITGLPQDADDAMVFHAGTQLVDGVVRTSGGRVLCVTALADSVKQAQQR
VYDVARGIHFDGAQYRHDIGHRAVKANG

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=428, Percent_Identity=51.1682242990654, Blast_Score=407, Evalue=1e-113,
Organism=Homo sapiens, GI209869995, Length=428, Percent_Identity=51.1682242990654, Blast_Score=407, Evalue=1e-113,
Organism=Homo sapiens, GI209869993, Length=428, Percent_Identity=51.1682242990654, Blast_Score=407, Evalue=1e-113,
Organism=Homo sapiens, GI28416899, Length=428, Percent_Identity=51.1682242990654, Blast_Score=406, Evalue=1e-113,
Organism=Escherichia coli, GI1790438, Length=429, Percent_Identity=62.2377622377622, Blast_Score=525, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI17567511, Length=425, Percent_Identity=41.6470588235294, Blast_Score=322, Evalue=2e-88,
Organism=Saccharomyces cerevisiae, GI6321203, Length=446, Percent_Identity=43.9461883408072, Blast_Score=351, Evalue=1e-97,
Organism=Drosophila melanogaster, GI62471619, Length=435, Percent_Identity=47.816091954023, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24582400, Length=435, Percent_Identity=47.816091954023, Blast_Score=359, Evalue=2e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45652; Mature: 45652

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVIGGGGREHALAWKLSQSPKTSKVYVAPGNGGTALSPKYENVPISDVTALREWAQA
CEEEEEECCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
QKIALTVVGPEAPLAAGVVDEFRTHGLRIFGPTQAAAQLESSKAFSKAFMKRHGIPTAEY
CEEEEEEECCCCCCHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCC
ETFTDPQAAHAYVNRLGAPIVIKADGLAAGKGVVVAMTLQEAHDAVDFMLVDNKYGVAHN
CCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEHHHCCCEEEEEEECCCCCEEC
EGGARVVIEQFLEGEEASFIVLCDGKNVLALATSQDHKRLKDGDQGPNTGGMGAYSPAPV
CCCCHHHHHHHHCCCCCCEEEEECCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCE
VTADVHARAMREVILPTIRGMEKDGIPYTGFLYAGLMIDATGHPKTLEFNCRMGDPETQP
EEEHHHHHHHHHHHHHHHCCCCCCCCCEEHHEEEEEEEECCCCCCEEEEEEECCCCCCCH
ILMRLKSDLVEVLGAAVDGKLDQVELQWDRRTALGVVMAAQGYPENPRKGDAITGLPQDA
HHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCC
DDAMVFHAGTQLVDGVVRTSGGRVLCVTALADSVKQAQQRVYDVARGIHFDGAQYRHDIG
CCCEEEECCHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHC
HRAVKANG
CCEEECCC
>Mature Secondary Structure
MKILVIGGGGREHALAWKLSQSPKTSKVYVAPGNGGTALSPKYENVPISDVTALREWAQA
CEEEEEECCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
QKIALTVVGPEAPLAAGVVDEFRTHGLRIFGPTQAAAQLESSKAFSKAFMKRHGIPTAEY
CEEEEEEECCCCCCHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCC
ETFTDPQAAHAYVNRLGAPIVIKADGLAAGKGVVVAMTLQEAHDAVDFMLVDNKYGVAHN
CCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEHHHCCCEEEEEEECCCCCEEC
EGGARVVIEQFLEGEEASFIVLCDGKNVLALATSQDHKRLKDGDQGPNTGGMGAYSPAPV
CCCCHHHHHHHHCCCCCCEEEEECCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCE
VTADVHARAMREVILPTIRGMEKDGIPYTGFLYAGLMIDATGHPKTLEFNCRMGDPETQP
EEEHHHHHHHHHHHHHHHCCCCCCCCCEEHHEEEEEEEECCCCCCEEEEEEECCCCCCCH
ILMRLKSDLVEVLGAAVDGKLDQVELQWDRRTALGVVMAAQGYPENPRKGDAITGLPQDA
HHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCC
DDAMVFHAGTQLVDGVVRTSGGRVLCVTALADSVKQAQQRVYDVARGIHFDGAQYRHDIG
CCCEEEECCHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHC
HRAVKANG
CCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]