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The map label for this gene is rlmL [H]

Identifier: 222110855

GI number: 222110855

Start: 1755568

End: 1756527

Strand: Direct

Name: rlmL [H]

Synonym: Dtpsy_1661

Alternate gene names: 222110855

Gene position: 1755568-1756527 (Clockwise)

Preceding gene: 222110853

Following gene: 222110856

Centisome position: 46.24

GC content: 69.17

Gene sequence:

>960_bases
ATGCAAGCCCTGCTCGACGCCATCGCCACCCTCACTCCGCCCGTTGAGGCCTGCCGCATCTTCCATGGGCGTGGCGGGCG
GTTTCCCGGCAGCGAGCAGTGGACGCTGGATGCCTATCCACCCGTGCTGGTGCTCACCAGCTTCGCCCCGGTGACGGACG
ACGCACTCGCCACCATCGACGGCGCCCTGCGCGCACGCTGGCAGCAGGTGGCGCCAGGGCAGCCGCTGAACTGGGTGTTC
CAGCACCGCGGCGAGGCGCTGCGCATCGAGGGCCGCAGCGATACGCGGCTGATGGCGGGCGAGGTGCCCGAGCCGCATGT
GGTCACCGAGGATGGCGCACGCCTGCGCGTCCAGGTGCTGCGCGGGCAGAACCACGGGTTGTTCCTGGACATGGCCGAAG
GGCGTCGCTGGGTGCGCGCGCATGTGGCGAACCACCGCGCACGCACCGGGCGCGGCGCTCGGGTGCTCAACCTGTTCGCC
TATACCTGTGCGTTTTCGGTCGCTGCGCTGCAGGCGGGCGCGGCAGAGGTGGTGAATGTGGACATGAGCCGGGGTGCCAT
AGCCACCGGGCAGCAGAACCACCAGCTCAACGGGCTGGGCGCCGGTGCCCGCTTTCTGGCGCATGACATCTTCAGCTCGT
GGGGCAAGATCACGCGCAGCGGGCCGTACGACCTGGTGATCGTGGATCCGCCGTCCTACCAGAAGGGCAGCTTCGTCGCG
ACCAAGGACTATGCGCGGCTCATGCGGCGCCTGCCGGAACTGCTGATGCCGGGCGGCCACGCCCTGCTGTGCCTGAACGC
GCCGGAGCTCGGGATGGAGTTTCTGCGCGAGCAGATGCGATCCCAGGCGCCTTCGCTGGCGTTCGTGGAACGCGTTCCCA
ACCCGGCGGCGTTCGCCGATGTGGACGAGGAGCGGTCGCTGAAGGTACTGGTTTTCCGGGATACGGCGGCGGTGGGTTGA

Upstream 100 bases:

>100_bases
AAACCGTGACGATAACGTAAAAACCATAGCTGTTAGCGCTGGCACAATCTGCGTTAGAGGGCATTTTGATGCCTATTTTT
CTTTGCGCCCGACCCCACTC

Downstream 100 bases:

>100_bases
AGGGGCTCCGCGCGCCACAATGCGGTGGACACCGCTTCCCACCCACCGGTTGAAACGCAGCACATGAACACCCCTCGCAT
CGGACTGATCGGCGTAGGCC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MQALLDAIATLTPPVEACRIFHGRGGRFPGSEQWTLDAYPPVLVLTSFAPVTDDALATIDGALRARWQQVAPGQPLNWVF
QHRGEALRIEGRSDTRLMAGEVPEPHVVTEDGARLRVQVLRGQNHGLFLDMAEGRRWVRAHVANHRARTGRGARVLNLFA
YTCAFSVAALQAGAAEVVNVDMSRGAIATGQQNHQLNGLGAGARFLAHDIFSSWGKITRSGPYDLVIVDPPSYQKGSFVA
TKDYARLMRRLPELLMPGGHALLCLNAPELGMEFLREQMRSQAPSLAFVERVPNPAAFADVDEERSLKVLVFRDTAAVG

Sequences:

>Translated_319_residues
MQALLDAIATLTPPVEACRIFHGRGGRFPGSEQWTLDAYPPVLVLTSFAPVTDDALATIDGALRARWQQVAPGQPLNWVF
QHRGEALRIEGRSDTRLMAGEVPEPHVVTEDGARLRVQVLRGQNHGLFLDMAEGRRWVRAHVANHRARTGRGARVLNLFA
YTCAFSVAALQAGAAEVVNVDMSRGAIATGQQNHQLNGLGAGARFLAHDIFSSWGKITRSGPYDLVIVDPPSYQKGSFVA
TKDYARLMRRLPELLMPGGHALLCLNAPELGMEFLREQMRSQAPSLAFVERVPNPAAFADVDEERSLKVLVFRDTAAVG
>Mature_319_residues
MQALLDAIATLTPPVEACRIFHGRGGRFPGSEQWTLDAYPPVLVLTSFAPVTDDALATIDGALRARWQQVAPGQPLNWVF
QHRGEALRIEGRSDTRLMAGEVPEPHVVTEDGARLRVQVLRGQNHGLFLDMAEGRRWVRAHVANHRARTGRGARVLNLFA
YTCAFSVAALQAGAAEVVNVDMSRGAIATGQQNHQLNGLGAGARFLAHDIFSSWGKITRSGPYDLVIVDPPSYQKGSFVA
TKDYARLMRRLPELLMPGGHALLCLNAPELGMEFLREQMRSQAPSLAFVERVPNPAAFADVDEERSLKVLVFRDTAAVG

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=256, Percent_Identity=28.125, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787181, Length=180, Percent_Identity=31.1111111111111, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 34878; Mature: 34878

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQALLDAIATLTPPVEACRIFHGRGGRFPGSEQWTLDAYPPVLVLTSFAPVTDDALATID
CHHHHHHHHHCCCCHHHHHHEECCCCCCCCCCCEEECCCCCEEEEECCCCCCCCHHHHHH
GALRARWQQVAPGQPLNWVFQHRGEALRIEGRSDTRLMAGEVPEPHVVTEDGARLRVQVL
HHHHHHHHHCCCCCCHHHHHHCCCCEEEEECCCCCEEEECCCCCCCEECCCCCEEEEEEE
RGQNHGLFLDMAEGRRWVRAHVANHRARTGRGARVLNLFAYTCAFSVAALQAGAAEVVNV
ECCCCCEEEEHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHEEEE
DMSRGAIATGQQNHQLNGLGAGARFLAHDIFSSWGKITRSGPYDLVIVDPPSYQKGSFVA
ECCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEE
TKDYARLMRRLPELLMPGGHALLCLNAPELGMEFLREQMRSQAPSLAFVERVPNPAAFAD
HHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEHHHHCCCCCCCCC
VDEERSLKVLVFRDTAAVG
CCCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MQALLDAIATLTPPVEACRIFHGRGGRFPGSEQWTLDAYPPVLVLTSFAPVTDDALATID
CHHHHHHHHHCCCCHHHHHHEECCCCCCCCCCCEEECCCCCEEEEECCCCCCCCHHHHHH
GALRARWQQVAPGQPLNWVFQHRGEALRIEGRSDTRLMAGEVPEPHVVTEDGARLRVQVL
HHHHHHHHHCCCCCCHHHHHHCCCCEEEEECCCCCEEEECCCCCCCEECCCCCEEEEEEE
RGQNHGLFLDMAEGRRWVRAHVANHRARTGRGARVLNLFAYTCAFSVAALQAGAAEVVNV
ECCCCCEEEEHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHEEEE
DMSRGAIATGQQNHQLNGLGAGARFLAHDIFSSWGKITRSGPYDLVIVDPPSYQKGSFVA
ECCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEE
TKDYARLMRRLPELLMPGGHALLCLNAPELGMEFLREQMRSQAPSLAFVERVPNPAAFAD
HHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEHHHHCCCCCCCCC
VDEERSLKVLVFRDTAAVG
CCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA