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The map label for this gene is lpdA [H]

Identifier: 222110853

GI number: 222110853

Start: 1753135

End: 1754994

Strand: Direct

Name: lpdA [H]

Synonym: Dtpsy_1659

Alternate gene names: 222110853

Gene position: 1753135-1754994 (Clockwise)

Preceding gene: 222110852

Following gene: 222110855

Centisome position: 46.18

GC content: 68.28

Gene sequence:

>1860_bases
ATGGCAATCATCGACATCAAGGTGCCCGACATCGGCGACTTCGCCGAGGTGGGCGTGATCGAAGTGCTGGTGCAGCCGGG
CGACACCATCCGGGCCGAGCAGAGCCTGGTCACCGTGGAGTCCGACAAGGCCTCCATGGAGATTCCCAGCAGCCACGCCG
GGGTGGTCAAGGAGCTCAAGGTCAAGCTGGGCGACAAGGTGGCCGAGGGCTCCGTGCTGCTGACGCTGGAAGTGGCGCAG
GGTGCGGCGGCGCCGGCTGCGGCCCCTGCCGCGGCGAGCACACCCAGCGCACCGCCATCTGAGTCAAAACCGGCTCCAGC
GCCCGCTGTTCAAGCGCCAGCAGCTCCTAAAACCGAAGCAGCCAGCTTCGGCGGCAGTGCCGACGTGGAGTGCGATGTGC
TGGTGCTGGGTGGCGGCCCCGGCGGTTACAGCGCCGCCTTCCGCGCCGCCGACCTGGGCCTGAACGTGGTGCTGGTCGAG
CGCTACGCCACCCTGGGCGGCGTGTGCCTGAACGTGGGCTGCATTCCCTCCAAGGCGCTGCTGCACGTGGCCGCGGTGAT
GGACGAGGTGAGCCACCTGAAGAGCGCCGGCATCGACTTCGGCGCGCCGCAGGTCCACATCGACACCCTGCGCGGCCACA
AGGAAAAGGTCATCGGCAAGCTGACCGGCGGGCTGGCGCAGATGGCCAAGATGCGCAAGGTGACCGTGCTGCGCGGCTAC
GGCCACTTCGTCGGCGCCAACCACCTGGAGGTGGAGGAAACCACCGGCACCAGCCAGGACAAGACCGGCGCCAAGAAGGT
GGTGGCGTTCAAGCGCGCCATCATCGCCGCCGGCAGCCAGGCGGTGCGCCTGCCCTTTATGCCCGACGATCCTCGCGTGG
TGGACTCGACCGGCGCGCTGGCGCTGCAGGGCGTGCCCAAACGCATGCTGATCCTGGGCGGCGGCATCATCGGCCTGGAA
ATGGGCACGGTGTACAGCACGCTGGGCGCGCGCCTGGATGTGGTGGAGATGATGGACGGCCTGATGCAGGGCGCCGACCG
CGACCTGGTCAAGGTATGGCAGAAGATGAACCAGCACCGTTTCGACAACGTGATGCTGAATACCAAGACGGTGGCCGCCG
AGGCCACGCCCGAAGGTATCAAGGTGAGCTTCGCGCCCGCCAAGGACGGCGTCACGGTGCCCGAGCCCCAGGTGTACGAC
CTGGTGCTGCAGGCCGTGGGTCGCACGCCCAACGGCAAGAAGATCGCCGCCGAGAAGGCCGGCGTCGCCGTGACGGACCG
CGGCTTCATCAACGTCGACATCCAGATGCGCACCAACGTGCCGCACATCTTCGCCATCGGCGACATCGTGGGCCAGCCCA
TGCTTGCGCACAAGGCGGTGCACGAGGCGCACGTGGCGGCCGAGGTCATCGCCGGTGAACTGCAGGGCAACAAGGAACTG
GCAAGCGCCGCCTTCAACGCCCGCGTGATCCCGAGCGTGGCCTATACCGACCCCGAAGTGGCTTGGGTGGGCCTGACCGA
GGACCAGGCCAAGGCGCAGGGCATCAAGGTCAAGAAAGGCCTGTTCCCCTGGGCGGCCAGCGGCCGCGCCATCGCCAACG
GCCGCGACGAAGGCTTTACCAAGCTGCTGTTCGACGACAGCCCCGAGGCGCACGGCCACGGCCGCATCCTGGGCGGCGGC
ATCGTCGGCACGCACGCCGGCGACATGATCGGCGAGATCGCCCTGGCCATCGAGATGGGCGCCGACGCGGTGGACATCGG
CAAGACCATCCACCCGCACCCCACGCTGGGCGAGAGCATCGGCATGGCGGCGGAGATTGCCCACGGCAGTTGCACGGACG
TGCCGCCGCAGCGCAGGTAA

Upstream 100 bases:

>100_bases
CGCGTGATCGACGGCGCCTCTGCCGCGCGCTTCAACGCCTACCTGGGTCAGGTGTTGGCGGACTACCGCCGCATCCTGCT
GTAAGGACGGAGCACAACAT

Downstream 100 bases:

>100_bases
AGGCCGCGGCCGTAGCAAAAAAGCAGCCTCTGGCTGCTTTTTTTATGCTTTTGATAGCGCCTAGCGCTTGCTGTGCAAGG
GCTGGAGGCGTTTTTTAGTA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein [H]

Number of amino acids: Translated: 619; Mature: 618

Protein sequence:

>619_residues
MAIIDIKVPDIGDFAEVGVIEVLVQPGDTIRAEQSLVTVESDKASMEIPSSHAGVVKELKVKLGDKVAEGSVLLTLEVAQ
GAAAPAAAPAAASTPSAPPSESKPAPAPAVQAPAAPKTEAASFGGSADVECDVLVLGGGPGGYSAAFRAADLGLNVVLVE
RYATLGGVCLNVGCIPSKALLHVAAVMDEVSHLKSAGIDFGAPQVHIDTLRGHKEKVIGKLTGGLAQMAKMRKVTVLRGY
GHFVGANHLEVEETTGTSQDKTGAKKVVAFKRAIIAAGSQAVRLPFMPDDPRVVDSTGALALQGVPKRMLILGGGIIGLE
MGTVYSTLGARLDVVEMMDGLMQGADRDLVKVWQKMNQHRFDNVMLNTKTVAAEATPEGIKVSFAPAKDGVTVPEPQVYD
LVLQAVGRTPNGKKIAAEKAGVAVTDRGFINVDIQMRTNVPHIFAIGDIVGQPMLAHKAVHEAHVAAEVIAGELQGNKEL
ASAAFNARVIPSVAYTDPEVAWVGLTEDQAKAQGIKVKKGLFPWAASGRAIANGRDEGFTKLLFDDSPEAHGHGRILGGG
IVGTHAGDMIGEIALAIEMGADAVDIGKTIHPHPTLGESIGMAAEIAHGSCTDVPPQRR

Sequences:

>Translated_619_residues
MAIIDIKVPDIGDFAEVGVIEVLVQPGDTIRAEQSLVTVESDKASMEIPSSHAGVVKELKVKLGDKVAEGSVLLTLEVAQ
GAAAPAAAPAAASTPSAPPSESKPAPAPAVQAPAAPKTEAASFGGSADVECDVLVLGGGPGGYSAAFRAADLGLNVVLVE
RYATLGGVCLNVGCIPSKALLHVAAVMDEVSHLKSAGIDFGAPQVHIDTLRGHKEKVIGKLTGGLAQMAKMRKVTVLRGY
GHFVGANHLEVEETTGTSQDKTGAKKVVAFKRAIIAAGSQAVRLPFMPDDPRVVDSTGALALQGVPKRMLILGGGIIGLE
MGTVYSTLGARLDVVEMMDGLMQGADRDLVKVWQKMNQHRFDNVMLNTKTVAAEATPEGIKVSFAPAKDGVTVPEPQVYD
LVLQAVGRTPNGKKIAAEKAGVAVTDRGFINVDIQMRTNVPHIFAIGDIVGQPMLAHKAVHEAHVAAEVIAGELQGNKEL
ASAAFNARVIPSVAYTDPEVAWVGLTEDQAKAQGIKVKKGLFPWAASGRAIANGRDEGFTKLLFDDSPEAHGHGRILGGG
IVGTHAGDMIGEIALAIEMGADAVDIGKTIHPHPTLGESIGMAAEIAHGSCTDVPPQRR
>Mature_618_residues
AIIDIKVPDIGDFAEVGVIEVLVQPGDTIRAEQSLVTVESDKASMEIPSSHAGVVKELKVKLGDKVAEGSVLLTLEVAQG
AAAPAAAPAAASTPSAPPSESKPAPAPAVQAPAAPKTEAASFGGSADVECDVLVLGGGPGGYSAAFRAADLGLNVVLVER
YATLGGVCLNVGCIPSKALLHVAAVMDEVSHLKSAGIDFGAPQVHIDTLRGHKEKVIGKLTGGLAQMAKMRKVTVLRGYG
HFVGANHLEVEETTGTSQDKTGAKKVVAFKRAIIAAGSQAVRLPFMPDDPRVVDSTGALALQGVPKRMLILGGGIIGLEM
GTVYSTLGARLDVVEMMDGLMQGADRDLVKVWQKMNQHRFDNVMLNTKTVAAEATPEGIKVSFAPAKDGVTVPEPQVYDL
VLQAVGRTPNGKKIAAEKAGVAVTDRGFINVDIQMRTNVPHIFAIGDIVGQPMLAHKAVHEAHVAAEVIAGELQGNKELA
SAAFNARVIPSVAYTDPEVAWVGLTEDQAKAQGIKVKKGLFPWAASGRAIANGRDEGFTKLLFDDSPEAHGHGRILGGGI
VGTHAGDMIGEIALAIEMGADAVDIGKTIHPHPTLGESIGMAAEIAHGSCTDVPPQRR

Specific function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=477, Percent_Identity=39.4129979035639, Blast_Score=313, Evalue=3e-85,
Organism=Homo sapiens, GI50301238, Length=484, Percent_Identity=27.8925619834711, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI291045266, Length=469, Percent_Identity=27.7185501066098, Blast_Score=136, Evalue=8e-32,
Organism=Homo sapiens, GI148277071, Length=452, Percent_Identity=26.5486725663717, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI148277065, Length=452, Percent_Identity=26.5486725663717, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI33519430, Length=452, Percent_Identity=26.5486725663717, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI33519428, Length=452, Percent_Identity=26.5486725663717, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI33519426, Length=452, Percent_Identity=26.5486725663717, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI291045268, Length=462, Percent_Identity=26.8398268398268, Blast_Score=116, Evalue=7e-26,
Organism=Homo sapiens, GI22035672, Length=485, Percent_Identity=27.4226804123711, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI1786307, Length=492, Percent_Identity=60.1626016260163, Blast_Score=556, Evalue=1e-159,
Organism=Escherichia coli, GI87082354, Length=488, Percent_Identity=26.6393442622951, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI87081717, Length=474, Percent_Identity=25.5274261603376, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI1789915, Length=466, Percent_Identity=29.1845493562232, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1786305, Length=74, Percent_Identity=59.4594594594595, Blast_Score=87, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI32565766, Length=474, Percent_Identity=38.8185654008439, Blast_Score=308, Evalue=4e-84,
Organism=Caenorhabditis elegans, GI17557007, Length=497, Percent_Identity=27.1629778672032, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI71983429, Length=473, Percent_Identity=24.9471458773784, Blast_Score=99, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI71983419, Length=473, Percent_Identity=24.9471458773784, Blast_Score=99, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71982272, Length=460, Percent_Identity=24.7826086956522, Blast_Score=93, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6321091, Length=480, Percent_Identity=39.1666666666667, Blast_Score=297, Evalue=3e-81,
Organism=Saccharomyces cerevisiae, GI6325240, Length=496, Percent_Identity=28.2258064516129, Blast_Score=180, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6325166, Length=492, Percent_Identity=25.4065040650407, Blast_Score=134, Evalue=5e-32,
Organism=Drosophila melanogaster, GI21358499, Length=494, Percent_Identity=39.6761133603239, Blast_Score=322, Evalue=7e-88,
Organism=Drosophila melanogaster, GI24640551, Length=547, Percent_Identity=27.9707495429616, Blast_Score=117, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24640553, Length=514, Percent_Identity=27.6264591439689, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24640549, Length=504, Percent_Identity=27.7777777777778, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI17737741, Length=493, Percent_Identity=25.3549695740365, Blast_Score=102, Evalue=8e-22,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 64148; Mature: 64017

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIIDIKVPDIGDFAEVGVIEVLVQPGDTIRAEQSLVTVESDKASMEIPSSHAGVVKELK
CEEEEEECCCCCCHHHCCEEEEEECCCCCEECCCEEEEEECCCCCEECCCCCHHHHHHHH
VKLGDKVAEGSVLLTLEVAQGAAAPAAAPAAASTPSAPPSESKPAPAPAVQAPAAPKTEA
HHHCCEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
ASFGGSADVECDVLVLGGGPGGYSAAFRAADLGLNVVLVERYATLGGVCLNVGCIPSKAL
HHCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEEHHHHHCCEEEEECCCCHHHH
LHVAAVMDEVSHLKSAGIDFGAPQVHIDTLRGHKEKVIGKLTGGLAQMAKMRKVTVLRGY
HHHHHHHHHHHHHHHCCCCCCCCEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHEEEEECC
GHFVGANHLEVEETTGTSQDKTGAKKVVAFKRAIIAAGSQAVRLPFMPDDPRVVDSTGAL
CCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEECCCCE
ALQGVPKRMLILGGGIIGLEMGTVYSTLGARLDVVEMMDGLMQGADRDLVKVWQKMNQHR
EECCCCCEEEEEECCEEEEEHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FDNVMLNTKTVAAEATPEGIKVSFAPAKDGVTVPEPQVYDLVLQAVGRTPNGKKIAAEKA
CCCEEEECEEEEECCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEHHCC
GVAVTDRGFINVDIQMRTNVPHIFAIGDIVGQPMLAHKAVHEAHVAAEVIAGELQGNKEL
CEEEECCCEEEEEEEEECCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHH
ASAAFNARVIPSVAYTDPEVAWVGLTEDQAKAQGIKVKKGLFPWAASGRAIANGRDEGFT
HHHHCCCEECCEEEECCCCEEEEECCCHHHHHCCEEEECCCCCCCCCCCEEECCCCCCCE
KLLFDDSPEAHGHGRILGGGIVGTHAGDMIGEIALAIEMGADAVDIGKTIHPHPTLGESI
EEEECCCCCCCCCCEEEECCEECCCCHHHHHHEEEEEECCCCHHHCCCCCCCCCCCCHHC
GMAAEIAHGSCTDVPPQRR
CCCEEECCCCCCCCCCCCC
>Mature Secondary Structure 
AIIDIKVPDIGDFAEVGVIEVLVQPGDTIRAEQSLVTVESDKASMEIPSSHAGVVKELK
EEEEEECCCCCCHHHCCEEEEEECCCCCEECCCEEEEEECCCCCEECCCCCHHHHHHHH
VKLGDKVAEGSVLLTLEVAQGAAAPAAAPAAASTPSAPPSESKPAPAPAVQAPAAPKTEA
HHHCCEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
ASFGGSADVECDVLVLGGGPGGYSAAFRAADLGLNVVLVERYATLGGVCLNVGCIPSKAL
HHCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEEHHHHHCCEEEEECCCCHHHH
LHVAAVMDEVSHLKSAGIDFGAPQVHIDTLRGHKEKVIGKLTGGLAQMAKMRKVTVLRGY
HHHHHHHHHHHHHHHCCCCCCCCEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHEEEEECC
GHFVGANHLEVEETTGTSQDKTGAKKVVAFKRAIIAAGSQAVRLPFMPDDPRVVDSTGAL
CCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEECCCCE
ALQGVPKRMLILGGGIIGLEMGTVYSTLGARLDVVEMMDGLMQGADRDLVKVWQKMNQHR
EECCCCCEEEEEECCEEEEEHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FDNVMLNTKTVAAEATPEGIKVSFAPAKDGVTVPEPQVYDLVLQAVGRTPNGKKIAAEKA
CCCEEEECEEEEECCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEHHCC
GVAVTDRGFINVDIQMRTNVPHIFAIGDIVGQPMLAHKAVHEAHVAAEVIAGELQGNKEL
CEEEECCCEEEEEEEEECCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHH
ASAAFNARVIPSVAYTDPEVAWVGLTEDQAKAQGIKVKKGLFPWAASGRAIANGRDEGFT
HHHHCCCEECCEEEECCCCEEEEECCCHHHHHCCEEEECCCCCCCCCCCEEECCCCCCCE
KLLFDDSPEAHGHGRILGGGIVGTHAGDMIGEIALAIEMGADAVDIGKTIHPHPTLGESI
EEEECCCCCCCCCCEEEECCEECCCCHHHHHHEEEEEECCCCHHHCCCCCCCCCCCCHHC
GMAAEIAHGSCTDVPPQRR
CCCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]