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The map label for this gene is pdhB [H]

Identifier: 222110852

GI number: 222110852

Start: 1751433

End: 1753118

Strand: Direct

Name: pdhB [H]

Synonym: Dtpsy_1658

Alternate gene names: 222110852

Gene position: 1751433-1753118 (Clockwise)

Preceding gene: 222110851

Following gene: 222110853

Centisome position: 46.13

GC content: 67.02

Gene sequence:

>1686_bases
ATGGCATTGGTAAACATCCAGGTTCCCGACATCGGCGACTTCGACGAAGTCGGCGTGATCGAGCTGCTGGTCAAGGTGGG
CGACACGGTGAAGGCGGAGCAGTCGCTGATCACCGTGGAGTCCGACAAGGCCTCGATGGAAATCCCGTCGAGCCACGCGG
GTGTGGTCAAGGAACTCAAGGTCCAGCTGGGCGACAAGGTCAAGCAGGGCAGCGTGATCGCCGTGCTGGAGGCCACGGGC
GAAGCCGCTGCTTCTGATGAAAAACCGGCTCCAGCGCCCGCCGCGCAAGCGCCGGCAGCTATCGAAACGCAAGCGCCTGC
CGCGCCTGCTGCACCCGCCGCCCCTGCTGCGGGTGGTGGCGCGGTTGAGGTGCGTGTGCCCGACATCGGTGACTTCAAGG
ACGTCGCCGTGATCGAGTTGCTGGTCCAGCCCGGCGATGCGGTCAAGGTGGAGCAGTCGCTGTTCACCGTGGAGTCCGAC
AAGGCCTCGATGGAGATCCCTTCGCCCGCCGCCGGCGTTCTCAAGGAGCTCAAGGTCAAGATCGGCGACAAGATCAATGT
GGGCGATCTCGTGGCGGTGCTTGAAGGTGCCGCCGCTGCACCGGCTGCCGCCCCGGCGCAGGGTTCTACACCAGCGGCCG
CTTCCGCTCCGGCCGCTGCCGCGCCCGTGGCGGCTCAGGCCTCCGCGCCCGCACCAGCTGCGGCAACGGCAACGGCGCCC
GTGCCGGCCCACCAGCCCTCGACGCCCACGCCGGGCCTGCCGCACGCCAGCCCCTCGGTGCGCAAGTTCGCCCGCGAACT
GGGTGTGCCGCTGGCGGAGGTCAAGGGCACGGGCATCAAGGGCCGCATCACGCAGGAGGATGTGGCGGCCTTCACGCGCC
AGGTGATGAGCGGTGCGCTGCAGACCCAGGCACAGGGCAGCAAGGCGCCCGCTGCGGGTGGCTCCGGGGTGGGCCTGGAC
CTGCTGCCGTGGCCCAAGGTGGACTTCGCCAAGTTCGGCCCCGTCGAGCGCAAGGAGCTGTCGCGCATCAAGAAGATCAG
CGGCGCCAACCTGTCGCGCAACTGGGTGATGATTCCCCACGTCACCAACAACGACGAGGCCGACATCACCGAACTGGAAG
CCTTCCGCGTCTCCACCAACAAGGAGAACGAGAAGAGCGGCGTCAAGGTCACCATGCTGGCCTTCGTCATCAAGGCCGTG
GTCGCGGCGCTGAAGAAGTTCCCCGAGTTCAACGCCAGCCTGGACGGCGACGCGCTGGTCTACAAGCAGTACTTCCACAT
CGGCTTTGCGGCCGACACGCCCAACGGCCTGGTGGTGCCTGTGCTGAAAGATGCCGACAAGAAGGGCATCCTGCAAATCA
GCGCCGAGATGGCCGAGCTGGCCAAGAAGGCCCGCGACGGCAAGCTGGGTGCGGCCGACATGCAGGGCGGCAGCATGTCG
ATCAGCTCGCTGGGCGGCATTGGCGGCACGCATTTCACCCCCATCATCAATGCGCCCGAGGTGGCCATCCTCGGCCTGTC
CAAGAGCCAGATGAAGCCCGTGTGGGACGGCCAGCAGTTCGTGCCGCGCCTCACGCTGCCGCTGAGCCTGTCGTACGACC
ACCGCGTGATCGACGGCGCCTCTGCCGCGCGCTTCAACGCCTACCTGGGTCAGGTGTTGGCGGACTACCGCCGCATCCTG
CTGTAA

Upstream 100 bases:

>100_bases
TAAGCTGCCCAAGGCGAAGGTGGCCGAGGCGATTGCCAAGTACGGCATCCAGACCGAGAAGATCAACCCCTTGCACGCCT
GATGGCCCGGGAGACACGAC

Downstream 100 bases:

>100_bases
GGACGGAGCACAACATATGGCAATCATCGACATCAAGGTGCCCGACATCGGCGACTTCGCCGAGGTGGGCGTGATCGAAG
TGCTGGTGCAGCCGGGCGAC

Product: pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 561; Mature: 560

Protein sequence:

>561_residues
MALVNIQVPDIGDFDEVGVIELLVKVGDTVKAEQSLITVESDKASMEIPSSHAGVVKELKVQLGDKVKQGSVIAVLEATG
EAAASDEKPAPAPAAQAPAAIETQAPAAPAAPAAPAAGGGAVEVRVPDIGDFKDVAVIELLVQPGDAVKVEQSLFTVESD
KASMEIPSPAAGVLKELKVKIGDKINVGDLVAVLEGAAAAPAAAPAQGSTPAAASAPAAAAPVAAQASAPAPAAATATAP
VPAHQPSTPTPGLPHASPSVRKFARELGVPLAEVKGTGIKGRITQEDVAAFTRQVMSGALQTQAQGSKAPAAGGSGVGLD
LLPWPKVDFAKFGPVERKELSRIKKISGANLSRNWVMIPHVTNNDEADITELEAFRVSTNKENEKSGVKVTMLAFVIKAV
VAALKKFPEFNASLDGDALVYKQYFHIGFAADTPNGLVVPVLKDADKKGILQISAEMAELAKKARDGKLGAADMQGGSMS
ISSLGGIGGTHFTPIINAPEVAILGLSKSQMKPVWDGQQFVPRLTLPLSLSYDHRVIDGASAARFNAYLGQVLADYRRIL
L

Sequences:

>Translated_561_residues
MALVNIQVPDIGDFDEVGVIELLVKVGDTVKAEQSLITVESDKASMEIPSSHAGVVKELKVQLGDKVKQGSVIAVLEATG
EAAASDEKPAPAPAAQAPAAIETQAPAAPAAPAAPAAGGGAVEVRVPDIGDFKDVAVIELLVQPGDAVKVEQSLFTVESD
KASMEIPSPAAGVLKELKVKIGDKINVGDLVAVLEGAAAAPAAAPAQGSTPAAASAPAAAAPVAAQASAPAPAAATATAP
VPAHQPSTPTPGLPHASPSVRKFARELGVPLAEVKGTGIKGRITQEDVAAFTRQVMSGALQTQAQGSKAPAAGGSGVGLD
LLPWPKVDFAKFGPVERKELSRIKKISGANLSRNWVMIPHVTNNDEADITELEAFRVSTNKENEKSGVKVTMLAFVIKAV
VAALKKFPEFNASLDGDALVYKQYFHIGFAADTPNGLVVPVLKDADKKGILQISAEMAELAKKARDGKLGAADMQGGSMS
ISSLGGIGGTHFTPIINAPEVAILGLSKSQMKPVWDGQQFVPRLTLPLSLSYDHRVIDGASAARFNAYLGQVLADYRRIL
L
>Mature_560_residues
ALVNIQVPDIGDFDEVGVIELLVKVGDTVKAEQSLITVESDKASMEIPSSHAGVVKELKVQLGDKVKQGSVIAVLEATGE
AAASDEKPAPAPAAQAPAAIETQAPAAPAAPAAPAAGGGAVEVRVPDIGDFKDVAVIELLVQPGDAVKVEQSLFTVESDK
ASMEIPSPAAGVLKELKVKIGDKINVGDLVAVLEGAAAAPAAAPAQGSTPAAASAPAAAAPVAAQASAPAPAAATATAPV
PAHQPSTPTPGLPHASPSVRKFARELGVPLAEVKGTGIKGRITQEDVAAFTRQVMSGALQTQAQGSKAPAAGGSGVGLDL
LPWPKVDFAKFGPVERKELSRIKKISGANLSRNWVMIPHVTNNDEADITELEAFRVSTNKENEKSGVKVTMLAFVIKAVV
AALKKFPEFNASLDGDALVYKQYFHIGFAADTPNGLVVPVLKDADKKGILQISAEMAELAKKARDGKLGAADMQGGSMSI
SSLGGIGGTHFTPIINAPEVAILGLSKSQMKPVWDGQQFVPRLTLPLSLSYDHRVIDGASAARFNAYLGQVLADYRRILL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=457, Percent_Identity=29.5404814004376, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI31711992, Length=331, Percent_Identity=32.3262839879154, Blast_Score=142, Evalue=1e-33,
Organism=Homo sapiens, GI203098816, Length=490, Percent_Identity=26.3265306122449, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI203098753, Length=430, Percent_Identity=26.9767441860465, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI19923748, Length=231, Percent_Identity=33.3333333333333, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI260898739, Length=151, Percent_Identity=37.0860927152318, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786305, Length=563, Percent_Identity=53.4635879218472, Blast_Score=515, Evalue=1e-147,
Organism=Escherichia coli, GI1786946, Length=445, Percent_Identity=30.7865168539326, Blast_Score=187, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17537937, Length=433, Percent_Identity=29.7921478060046, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17560088, Length=454, Percent_Identity=29.7356828193833, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI25146366, Length=210, Percent_Identity=39.0476190476191, Blast_Score=135, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17538894, Length=219, Percent_Identity=34.2465753424658, Blast_Score=104, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6320352, Length=418, Percent_Identity=29.4258373205742, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6324258, Length=433, Percent_Identity=29.7921478060046, Blast_Score=128, Evalue=3e-30,
Organism=Drosophila melanogaster, GI18859875, Length=450, Percent_Identity=32.6666666666667, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24582497, Length=435, Percent_Identity=30.1149425287356, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI20129315, Length=203, Percent_Identity=36.4532019704434, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24645909, Length=215, Percent_Identity=33.953488372093, Blast_Score=116, Evalue=4e-26,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 57718; Mature: 57586

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVNIQVPDIGDFDEVGVIELLVKVGDTVKAEQSLITVESDKASMEIPSSHAGVVKELK
CEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEECCCCHHHHHHHHH
VQLGDKVKQGSVIAVLEATGEAAASDEKPAPAPAAQAPAAIETQAPAAPAAPAAPAAGGG
HHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AVEVRVPDIGDFKDVAVIELLVQPGDAVKVEQSLFTVESDKASMEIPSPAAGVLKELKVK
EEEEECCCCCCCHHHEEHHHEECCCCCEEEHHHHEEEECCCCCCCCCCCHHHHHHHHHHH
IGDKINVGDLVAVLEGAAAAPAAAPAQGSTPAAASAPAAAAPVAAQASAPAPAAATATAP
CCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCC
VPAHQPSTPTPGLPHASPSVRKFARELGVPLAEVKGTGIKGRITQEDVAAFTRQVMSGAL
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
QTQAQGSKAPAAGGSGVGLDLLPWPKVDFAKFGPVERKELSRIKKISGANLSRNWVMIPH
HHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEE
VTNNDEADITELEAFRVSTNKENEKSGVKVTMLAFVIKAVVAALKKFPEFNASLDGDALV
ECCCCCCCHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
YKQYFHIGFAADTPNGLVVPVLKDADKKGILQISAEMAELAKKARDGKLGAADMQGGSMS
EEEEEEEEEECCCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCEE
ISSLGGIGGTHFTPIINAPEVAILGLSKSQMKPVWDGQQFVPRLTLPLSLSYDHRVIDGA
HHHHCCCCCCCCCCCCCCCCEEEEECCHHHCCCCCCCHHHCCEEEEEEEECCCCEEECCC
SAARFNAYLGQVLADYRRILL
CHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALVNIQVPDIGDFDEVGVIELLVKVGDTVKAEQSLITVESDKASMEIPSSHAGVVKELK
EEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEECCCCHHHHHHHHH
VQLGDKVKQGSVIAVLEATGEAAASDEKPAPAPAAQAPAAIETQAPAAPAAPAAPAAGGG
HHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AVEVRVPDIGDFKDVAVIELLVQPGDAVKVEQSLFTVESDKASMEIPSPAAGVLKELKVK
EEEEECCCCCCCHHHEEHHHEECCCCCEEEHHHHEEEECCCCCCCCCCCHHHHHHHHHHH
IGDKINVGDLVAVLEGAAAAPAAAPAQGSTPAAASAPAAAAPVAAQASAPAPAAATATAP
CCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCC
VPAHQPSTPTPGLPHASPSVRKFARELGVPLAEVKGTGIKGRITQEDVAAFTRQVMSGAL
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
QTQAQGSKAPAAGGSGVGLDLLPWPKVDFAKFGPVERKELSRIKKISGANLSRNWVMIPH
HHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEE
VTNNDEADITELEAFRVSTNKENEKSGVKVTMLAFVIKAVVAALKKFPEFNASLDGDALV
ECCCCCCCHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
YKQYFHIGFAADTPNGLVVPVLKDADKKGILQISAEMAELAKKARDGKLGAADMQGGSMS
EEEEEEEEEECCCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCEE
ISSLGGIGGTHFTPIINAPEVAILGLSKSQMKPVWDGQQFVPRLTLPLSLSYDHRVIDGA
HHHHCCCCCCCCCCCCCCCCEEEEECCHHHCCCCCCCHHHCCEEEEEEEECCCCEEECCC
SAARFNAYLGQVLADYRRILL
CHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8021225 [H]