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The map label for this gene is htrB [H]

Identifier: 222109901

GI number: 222109901

Start: 712272

End: 713144

Strand: Direct

Name: htrB [H]

Synonym: Dtpsy_0685

Alternate gene names: 222109901

Gene position: 712272-713144 (Clockwise)

Preceding gene: 222109900

Following gene: 222109902

Centisome position: 18.76

GC content: 67.81

Gene sequence:

>873_bases
ATGATGGCGCGCGCGGGCATCGCGCTGCTGAATCTGCTGGGTCGCCTGCCGCTGCCGATGCTGCGCGCGCTGGGGCGGTT
CATCGGCCGGGTGCTGTTCGTGCTGGCCGTGCCGCGGCGCAGGGTGGCGCTGCGCAACCTGGCCCTGTGTTTTCCGGAGG
TGCCCGAATCGCAGCGCCGCGCGTGGGCGCGCGAGTCCTTCGTGGTGTTCTGCCAGACCTTCCTGGACCGCGGCTGGTTG
TGGTCGGGCTCTGAGGCGCTGGTGCGCAGTCGCGTGAAGCTGGTGGGCGCGCTGCACGAGCTGGAAGGCGACACGCCAAC
CATCGTCTTCGCGCCGCACTTCTACAGCATGGACGCAGGCGGTCTCGCCTTGCCGCTGAACACACGGCGCGAGTTCACCT
CGATCTTCGCCACCAACCCCGACCCGGTGCTGGACGCATGGTTCATGGCGGGCCGCCAGCGCTTTGGCAACGTGCGCATG
CTCAACCGCGCCGACGGCGTGAAACCCATCATCTCGTGCCTGCGCAAGGGCGGGCTGCTGTACCTGCTGCCCGACATGGA
CTACGGCAGGAACGACTCGGTGTTCGTGCCCTTCTTCGCCATGCCGGACGCGGCCACCATCCCGTCACTGTCGCGCTTTG
CTCGCCTGGGGCGCGCCAAGGTGGTGGCGCTGTACAGCCGCATGACGCCCGAGGGTTACGTGGCCGAGCTGACCCCCGCC
TGGGACCACTTTCCCACCGAGGACCATGTGGCCGACACGGCGCGCATGAACCGTGAGCTGGAAGCCGCCATCCGCACCAT
GCCCGCGCAGTACTACTGGGTACACAAGCGCTTCAAGACCCGCCCGGAGGGACAGGATTCGGTGTATCAGTAA

Upstream 100 bases:

>100_bases
AAAGCCCCGACCAATACCTGTGGGGCTATGCCCGCTACAAGCAGCCCCGCGCAGAGATGCATACCTCTGCCGGGACCGAT
GCGCCGCCGGAGGGCCGCGC

Downstream 100 bases:

>100_bases
AAATGGCTCTAGCAGGCCGTTGAAAAATCCCCCTCCGACGCCCGCGTTCGCCGCTACGATCTGAAGCCATAGGTGAACGA
CCCAGACGAGGACGATGAGA

Product: lipid a biosynthesis acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MMARAGIALLNLLGRLPLPMLRALGRFIGRVLFVLAVPRRRVALRNLALCFPEVPESQRRAWARESFVVFCQTFLDRGWL
WSGSEALVRSRVKLVGALHELEGDTPTIVFAPHFYSMDAGGLALPLNTRREFTSIFATNPDPVLDAWFMAGRQRFGNVRM
LNRADGVKPIISCLRKGGLLYLLPDMDYGRNDSVFVPFFAMPDAATIPSLSRFARLGRAKVVALYSRMTPEGYVAELTPA
WDHFPTEDHVADTARMNRELEAAIRTMPAQYYWVHKRFKTRPEGQDSVYQ

Sequences:

>Translated_290_residues
MMARAGIALLNLLGRLPLPMLRALGRFIGRVLFVLAVPRRRVALRNLALCFPEVPESQRRAWARESFVVFCQTFLDRGWL
WSGSEALVRSRVKLVGALHELEGDTPTIVFAPHFYSMDAGGLALPLNTRREFTSIFATNPDPVLDAWFMAGRQRFGNVRM
LNRADGVKPIISCLRKGGLLYLLPDMDYGRNDSVFVPFFAMPDAATIPSLSRFARLGRAKVVALYSRMTPEGYVAELTPA
WDHFPTEDHVADTARMNRELEAAIRTMPAQYYWVHKRFKTRPEGQDSVYQ
>Mature_290_residues
MMARAGIALLNLLGRLPLPMLRALGRFIGRVLFVLAVPRRRVALRNLALCFPEVPESQRRAWARESFVVFCQTFLDRGWL
WSGSEALVRSRVKLVGALHELEGDTPTIVFAPHFYSMDAGGLALPLNTRREFTSIFATNPDPVLDAWFMAGRQRFGNVRM
LNRADGVKPIISCLRKGGLLYLLPDMDYGRNDSVFVPFFAMPDAATIPSLSRFARLGRAKVVALYSRMTPEGYVAELTPA
WDHFPTEDHVADTARMNRELEAAIRTMPAQYYWVHKRFKTRPEGQDSVYQ

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=289, Percent_Identity=32.5259515570934, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI87082094, Length=290, Percent_Identity=31.0344827586207, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1788161, Length=296, Percent_Identity=34.7972972972973, Blast_Score=129, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 32877; Mature: 32877

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMARAGIALLNLLGRLPLPMLRALGRFIGRVLFVLAVPRRRVALRNLALCFPEVPESQRR
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHH
AWARESFVVFCQTFLDRGWLWSGSEALVRSRVKLVGALHELEGDTPTIVFAPHFYSMDAG
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEECCC
GLALPLNTRREFTSIFATNPDPVLDAWFMAGRQRFGNVRMLNRADGVKPIISCLRKGGLL
CEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCEE
YLLPDMDYGRNDSVFVPFFAMPDAATIPSLSRFARLGRAKVVALYSRMTPEGYVAELTPA
EEECCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHCHHHHHHHHHHCCCCCCEEEECCC
WDHFPTEDHVADTARMNRELEAAIRTMPAQYYWVHKRFKTRPEGQDSVYQ
CCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MMARAGIALLNLLGRLPLPMLRALGRFIGRVLFVLAVPRRRVALRNLALCFPEVPESQRR
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHH
AWARESFVVFCQTFLDRGWLWSGSEALVRSRVKLVGALHELEGDTPTIVFAPHFYSMDAG
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEECCC
GLALPLNTRREFTSIFATNPDPVLDAWFMAGRQRFGNVRMLNRADGVKPIISCLRKGGLL
CEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCEE
YLLPDMDYGRNDSVFVPFFAMPDAATIPSLSRFARLGRAKVVALYSRMTPEGYVAELTPA
EEECCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHCHHHHHHHHHHCCCCCCEEEECCC
WDHFPTEDHVADTARMNRELEAAIRTMPAQYYWVHKRFKTRPEGQDSVYQ
CCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]