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The map label for this gene is metF [H]

Identifier: 222109815

GI number: 222109815

Start: 619622

End: 620461

Strand: Direct

Name: metF [H]

Synonym: Dtpsy_0599

Alternate gene names: 222109815

Gene position: 619622-620461 (Clockwise)

Preceding gene: 222109814

Following gene: 222109816

Centisome position: 16.32

GC content: 69.05

Gene sequence:

>840_bases
ATGAGCCTTTCCACGGCCTTTCCGGTCAGCTTTGAATTCTTCCCGCCCAAGACGCCCGAGGGCGCCGACAAACTGCGCGC
CGTGCGCCAGCAGCTGTATGCCCGCCGGCCCGAGTTCTGCTCCGTCACCTACGGCGCGGGGGGCTCCACGCAGGCGGGCA
CCTTCGGCACGGTGCGCGAGATCCTCGCCGAGGGCGTGAGCGCGGCCTCGCACTTCTCGTGCATCGGCGCCACCAAGGAC
GGCGTGCGCGCGCAGCTCGCCGAGCTGAAAGCCATGGGCGTGAAGCGCCTGGTTGCGCTGCGCGGCGACCTGCCCAGTGG
CTACGGCATCGGGGGCGAGTTCCATTACGCGAGCGATCTGGTGGCGTTCATCCGCGCCGAGACGGGGCGCGACTTCCACA
TCGAGGTGGCGGCCTACCCCGAGGTCCATCCCCAGGCCCGTTCGTCGGATGCCGACCTGCAGGCCTTTGCCACCAAGGTG
CGCGCGGGCGCCGACTCGGCCATCACACAGTACTTCTTCAATGCCGACGCCTACCTGCGCTTCGTGCAGGACGTGCGCCG
CCTGGGGCTGGACGTGCCCGTGGTGCCGGGCATCATGCCGATCATGGGCGCGTCGCAGCTGCTGCGCTTTTCGGACGCCT
GCGGCGCCGAGATCCCGCGCTGGATCCGCCTGCGCCTGCAGGGCTATGGTGACGATGTGGCGAGCATTCGCGCCTTCGGC
CTGGATGTCGTCACGCACCTGTGCGAGCAACTGCGCCGCGAGGGCGTGCCGGGCCTGCACTTCTACACCATGAACCAGAG
CGCGGCCACGTTGGAAATCTGCGACCGCCTGGGGCTCTGA

Upstream 100 bases:

>100_bases
GCTGCACGGAGCTGGGGCTGCACGTGGTGGCCTGGATCGATAGCCCCATCGAGGGGGGCGACGGCAACCGCGAGTTCTTC
ATCCATGCAAGGAGGAAGGC

Downstream 100 bases:

>100_bases
AGCAGCGGGGATGGGTAATATCGGCAGCTTTTTCCCGTCCTCTTCCTCCTATCCCCTGCGCGTTTGCGGTATGAGTCCCG
TCATTGCCTTCCTGCTGTTC

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MSLSTAFPVSFEFFPPKTPEGADKLRAVRQQLYARRPEFCSVTYGAGGSTQAGTFGTVREILAEGVSAASHFSCIGATKD
GVRAQLAELKAMGVKRLVALRGDLPSGYGIGGEFHYASDLVAFIRAETGRDFHIEVAAYPEVHPQARSSDADLQAFATKV
RAGADSAITQYFFNADAYLRFVQDVRRLGLDVPVVPGIMPIMGASQLLRFSDACGAEIPRWIRLRLQGYGDDVASIRAFG
LDVVTHLCEQLRREGVPGLHFYTMNQSAATLEICDRLGL

Sequences:

>Translated_279_residues
MSLSTAFPVSFEFFPPKTPEGADKLRAVRQQLYARRPEFCSVTYGAGGSTQAGTFGTVREILAEGVSAASHFSCIGATKD
GVRAQLAELKAMGVKRLVALRGDLPSGYGIGGEFHYASDLVAFIRAETGRDFHIEVAAYPEVHPQARSSDADLQAFATKV
RAGADSAITQYFFNADAYLRFVQDVRRLGLDVPVVPGIMPIMGASQLLRFSDACGAEIPRWIRLRLQGYGDDVASIRAFG
LDVVTHLCEQLRREGVPGLHFYTMNQSAATLEICDRLGL
>Mature_278_residues
SLSTAFPVSFEFFPPKTPEGADKLRAVRQQLYARRPEFCSVTYGAGGSTQAGTFGTVREILAEGVSAASHFSCIGATKDG
VRAQLAELKAMGVKRLVALRGDLPSGYGIGGEFHYASDLVAFIRAETGRDFHIEVAAYPEVHPQARSSDADLQAFATKVR
AGADSAITQYFFNADAYLRFVQDVRRLGLDVPVVPGIMPIMGASQLLRFSDACGAEIPRWIRLRLQGYGDDVASIRAFGL
DVVTHLCEQLRREGVPGLHFYTMNQSAATLEICDRLGL

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=279, Percent_Identity=38.7096774193548, Blast_Score=184, Evalue=1e-46,
Organism=Escherichia coli, GI1790377, Length=272, Percent_Identity=41.1764705882353, Blast_Score=212, Evalue=3e-56,
Organism=Caenorhabditis elegans, GI25147441, Length=278, Percent_Identity=34.5323741007194, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI25147436, Length=278, Percent_Identity=34.5323741007194, Blast_Score=163, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6321313, Length=281, Percent_Identity=33.4519572953737, Blast_Score=160, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6325234, Length=288, Percent_Identity=32.9861111111111, Blast_Score=136, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 30300; Mature: 30169

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSTAFPVSFEFFPPKTPEGADKLRAVRQQLYARRPEFCSVTYGAGGSTQAGTFGTVRE
CCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHH
ILAEGVSAASHFSCIGATKDGVRAQLAELKAMGVKRLVALRGDLPSGYGIGGEFHYASDL
HHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
VAFIRAETGRDFHIEVAAYPEVHPQARSSDADLQAFATKVRAGADSAITQYFFNADAYLR
HHHHHHCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCHHHHHH
FVQDVRRLGLDVPVVPGIMPIMGASQLLRFSDACGAEIPRWIRLRLQGYGDDVASIRAFG
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHEECCCCCCHHHHHHHH
LDVVTHLCEQLRREGVPGLHFYTMNQSAATLEICDRLGL
HHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SLSTAFPVSFEFFPPKTPEGADKLRAVRQQLYARRPEFCSVTYGAGGSTQAGTFGTVRE
CCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHH
ILAEGVSAASHFSCIGATKDGVRAQLAELKAMGVKRLVALRGDLPSGYGIGGEFHYASDL
HHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
VAFIRAETGRDFHIEVAAYPEVHPQARSSDADLQAFATKVRAGADSAITQYFFNADAYLR
HHHHHHCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCHHHHHH
FVQDVRRLGLDVPVVPGIMPIMGASQLLRFSDACGAEIPRWIRLRLQGYGDDVASIRAFG
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHEECCCCCCHHHHHHHH
LDVVTHLCEQLRREGVPGLHFYTMNQSAATLEICDRLGL
HHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]