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The map label for this gene is alkJ [H]

Identifier: 222109696

GI number: 222109696

Start: 491649

End: 493244

Strand: Direct

Name: alkJ [H]

Synonym: Dtpsy_0477

Alternate gene names: 222109696

Gene position: 491649-493244 (Clockwise)

Preceding gene: 222109688

Following gene: 222109697

Centisome position: 12.95

GC content: 70.24

Gene sequence:

>1596_bases
ATGCGTGAGTTCGACTATGTCGTGATCGGGGGCGGCTCGGCGGGCTGCGTGCTGGCGGGGCGGCTGAGCGAGGACCCCAC
GGTGCGCGTGTGCCTGTTGGAGGCGGGCGGCAGCGATGCCAGTGTGCTCATCCATTGCCCGGCGGGGCTGGCCGCCATGG
CGCGCAGCGGCGCCTTCAACTGGGGGCTGCACACCACGCCGCAGGCGGGCCTGGGCGGGCGTCGGGGCTACCAGCCGCGC
GGCAAGGTGCTGGGTGGTTCCAGCTCGGTCAACGCCATGATCTACGCGCGCGGCCACGCCAGCGACTACGACCACTGGGC
TGCGGCCGGCAATGCAGGCTGGGGCTGGAACGACGTGCTGCCGTACTTTCTCCGGGCCGAGCACAACGAGCGCGGTGCAA
GCGCCTGGCACGGCGCGGACGGGCCGCTGAATGTGGCGGACCTGCAGTCGCCGCAGCGTGCCAGCCGCGCCTTCGTCGAA
GCCGGCGTGCAGGCTGGCCATCCACGCAATGACGACTTCAACGGCGCGCAACTGGAGGGCGTGGGTCTCTACCAGGTCAC
GCACCGGGCGGGCGAACGCTTCAGCGTGGCCAAGGCCTACCTCACGCCCCACCTGGGCCGGACCAATCTGCAGGTGGTGA
CCGGCGCGCAGGCCACGCGCATCCTGTTTGCGGGCCGGCGCGCCACGGGCGTGGAATACCGCCGAGGCGGCCAGACGCAG
CAGGTGCGCGCCACGCGTGAGGTGCTGCTGTCGGCGGGGGCGCTGCTATCGCCGCAATTGCTCATGCTCTCGGGTGTGGG
GCCGGGCGCGCAGTTGCAGAGCCACGGCATTGGCGTGGTGCACGATTTGCCGGGCGTGGGCGCGCATCTGCACGACCATC
CCGACGTGGTGCAGGTGATGGACGCACCGCGGTTGACGGACCTCTTCGGCCTGTCGCTGCCCGGGGCGCTGAACGTGCTG
CGCGGCGTGCGGGAGTGGCGTGCGCACCGCAGCGGCATGCTGACCACCAACTTCGCCGAGGCTGGAGGCTTCATCAAGAG
TCGCCCCGAGGAGCCGTTGCCCGACCTGCAGCTGCACTTCGTGGTCGGCAAGCTGCTGGACCACGGCCGGCGGGCCACAT
GGGGCCATGGCTATTCCTGCCATGTATGCCTGCTGCAGCCCCGAAGCCGCGGCCGGGTGACGCTGGCCAGCGCCGACCCG
GCGGCCGCTCCGCTGGTGGACCCGGCATTCTTCAGTGATCCCGACGACATGCAACGCATGGTGCGCGGCGTGCGGCGCAT
GCGCGAGATCTTGGCGCAGCCCGCCCTGGCCAGCCTGGGCGCGCGTGAACTGCCCCATTCGTCCCGTGCGCAAAGCGATG
CACAGATCGAGCAGTTCATCCGCGACTGGGCCGACACCATCTACCACCCCGTGGGCACGTGCCGCATGGGCTGCGGTCCG
CTGGACGTGGTGGATGCCCAACTGCGTGTGCATGGCCTGCAGGCTCTGCGTGTGGTGGACGCATCCATCATGCCGCGCAT
TGTGAGCGGCAACACGAATGCACCCACGGTGATGATTGCCGAGCGCGCGGTGGACTTGCTGCGCCAGGCTGCATGA

Upstream 100 bases:

>100_bases
TGAAAGTGTAGCGGTGAGAAAAAAACGATGCCGTGATGGTATCGGCAATCGCGCGTCTGCTGCTGCGTTGCAGCATCGCC
ATACAACGAGGGGACACTGC

Downstream 100 bases:

>100_bases
CGCGCTCCGGTTCGGCAGTGCGCGCTTAAGAAATTTTGAGGGTTCGCATAAGCGTTTTTTAGCAGCACGCGCGCTGCACC
GTTCCTAGACTGCACAGCAC

Product: choline dehydrogenase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MREFDYVVIGGGSAGCVLAGRLSEDPTVRVCLLEAGGSDASVLIHCPAGLAAMARSGAFNWGLHTTPQAGLGGRRGYQPR
GKVLGGSSSVNAMIYARGHASDYDHWAAAGNAGWGWNDVLPYFLRAEHNERGASAWHGADGPLNVADLQSPQRASRAFVE
AGVQAGHPRNDDFNGAQLEGVGLYQVTHRAGERFSVAKAYLTPHLGRTNLQVVTGAQATRILFAGRRATGVEYRRGGQTQ
QVRATREVLLSAGALLSPQLLMLSGVGPGAQLQSHGIGVVHDLPGVGAHLHDHPDVVQVMDAPRLTDLFGLSLPGALNVL
RGVREWRAHRSGMLTTNFAEAGGFIKSRPEEPLPDLQLHFVVGKLLDHGRRATWGHGYSCHVCLLQPRSRGRVTLASADP
AAAPLVDPAFFSDPDDMQRMVRGVRRMREILAQPALASLGARELPHSSRAQSDAQIEQFIRDWADTIYHPVGTCRMGCGP
LDVVDAQLRVHGLQALRVVDASIMPRIVSGNTNAPTVMIAERAVDLLRQAA

Sequences:

>Translated_531_residues
MREFDYVVIGGGSAGCVLAGRLSEDPTVRVCLLEAGGSDASVLIHCPAGLAAMARSGAFNWGLHTTPQAGLGGRRGYQPR
GKVLGGSSSVNAMIYARGHASDYDHWAAAGNAGWGWNDVLPYFLRAEHNERGASAWHGADGPLNVADLQSPQRASRAFVE
AGVQAGHPRNDDFNGAQLEGVGLYQVTHRAGERFSVAKAYLTPHLGRTNLQVVTGAQATRILFAGRRATGVEYRRGGQTQ
QVRATREVLLSAGALLSPQLLMLSGVGPGAQLQSHGIGVVHDLPGVGAHLHDHPDVVQVMDAPRLTDLFGLSLPGALNVL
RGVREWRAHRSGMLTTNFAEAGGFIKSRPEEPLPDLQLHFVVGKLLDHGRRATWGHGYSCHVCLLQPRSRGRVTLASADP
AAAPLVDPAFFSDPDDMQRMVRGVRRMREILAQPALASLGARELPHSSRAQSDAQIEQFIRDWADTIYHPVGTCRMGCGP
LDVVDAQLRVHGLQALRVVDASIMPRIVSGNTNAPTVMIAERAVDLLRQAA
>Mature_531_residues
MREFDYVVIGGGSAGCVLAGRLSEDPTVRVCLLEAGGSDASVLIHCPAGLAAMARSGAFNWGLHTTPQAGLGGRRGYQPR
GKVLGGSSSVNAMIYARGHASDYDHWAAAGNAGWGWNDVLPYFLRAEHNERGASAWHGADGPLNVADLQSPQRASRAFVE
AGVQAGHPRNDDFNGAQLEGVGLYQVTHRAGERFSVAKAYLTPHLGRTNLQVVTGAQATRILFAGRRATGVEYRRGGQTQ
QVRATREVLLSAGALLSPQLLMLSGVGPGAQLQSHGIGVVHDLPGVGAHLHDHPDVVQVMDAPRLTDLFGLSLPGALNVL
RGVREWRAHRSGMLTTNFAEAGGFIKSRPEEPLPDLQLHFVVGKLLDHGRRATWGHGYSCHVCLLQPRSRGRVTLASADP
AAAPLVDPAFFSDPDDMQRMVRGVRRMREILAQPALASLGARELPHSSRAQSDAQIEQFIRDWADTIYHPVGTCRMGCGP
LDVVDAQLRVHGLQALRVVDASIMPRIVSGNTNAPTVMIAERAVDLLRQAA

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=538, Percent_Identity=47.2118959107807, Blast_Score=451, Evalue=1e-126,
Organism=Escherichia coli, GI1786503, Length=535, Percent_Identity=42.6168224299065, Blast_Score=397, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17532301, Length=544, Percent_Identity=38.2352941176471, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI45549471, Length=578, Percent_Identity=39.4463667820069, Blast_Score=341, Evalue=7e-94,
Organism=Drosophila melanogaster, GI45551458, Length=578, Percent_Identity=39.4463667820069, Blast_Score=341, Evalue=9e-94,
Organism=Drosophila melanogaster, GI24642055, Length=576, Percent_Identity=39.4097222222222, Blast_Score=335, Evalue=4e-92,
Organism=Drosophila melanogaster, GI24642037, Length=571, Percent_Identity=37.8283712784588, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24642042, Length=573, Percent_Identity=38.5689354275742, Blast_Score=329, Evalue=2e-90,
Organism=Drosophila melanogaster, GI24642035, Length=582, Percent_Identity=36.9415807560137, Blast_Score=319, Evalue=3e-87,
Organism=Drosophila melanogaster, GI24642048, Length=570, Percent_Identity=36.8421052631579, Blast_Score=318, Evalue=7e-87,
Organism=Drosophila melanogaster, GI24650267, Length=567, Percent_Identity=35.0970017636684, Blast_Score=315, Evalue=5e-86,
Organism=Drosophila melanogaster, GI24642059, Length=582, Percent_Identity=36.0824742268041, Blast_Score=313, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24642039, Length=565, Percent_Identity=35.7522123893805, Blast_Score=307, Evalue=1e-83,
Organism=Drosophila melanogaster, GI17137792, Length=561, Percent_Identity=36.8983957219251, Blast_Score=305, Evalue=4e-83,
Organism=Drosophila melanogaster, GI18859995, Length=570, Percent_Identity=35.9649122807018, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI18859993, Length=571, Percent_Identity=34.3257443082312, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24642051, Length=567, Percent_Identity=33.3333333333333, Blast_Score=270, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24645930, Length=591, Percent_Identity=29.2724196277496, Blast_Score=168, Evalue=9e-42,
Organism=Drosophila melanogaster, GI24642057, Length=587, Percent_Identity=29.6422487223169, Blast_Score=153, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 56835; Mature: 56835

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREFDYVVIGGGSAGCVLAGRLSEDPTVRVCLLEAGGSDASVLIHCPAGLAAMARSGAFN
CCCCCEEEEECCCCCEEEEECCCCCCCEEEEEEECCCCCCEEEEECCCCHHHHHHCCCCC
WGLHTTPQAGLGGRRGYQPRGKVLGGSSSVNAMIYARGHASDYDHWAAAGNAGWGWNDVL
CCCCCCCCCCCCCCCCCCCCCCEECCCCCCEEEEEECCCCCCCCHHHCCCCCCCCHHHHH
PYFLRAEHNERGASAWHGADGPLNVADLQSPQRASRAFVEAGVQAGHPRNDDFNGAQLEG
HHHHHCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEECC
VGLYQVTHRAGERFSVAKAYLTPHLGRTNLQVVTGAQATRILFAGRRATGVEYRRGGQTQ
CCHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEECCCEEEEEEECCCCCCCHHHCCCCHH
QVRATREVLLSAGALLSPQLLMLSGVGPGAQLQSHGIGVVHDLPGVGAHLHDHPDVVQVM
HHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHCCCEEEECCCCCCCCCCCCCCHHHHH
DAPRLTDLFGLSLPGALNVLRGVREWRAHRSGMLTTNFAEAGGFIKSRPEEPLPDLQLHF
CCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEECHHHHCCHHHCCCCCCCCCHHHHH
VVGKLLDHGRRATWGHGYSCHVCLLQPRSRGRVTLASADPAAAPLVDPAFFSDPDDMQRM
HHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCCCCCCCCCHHCCCHHHHHHH
VRGVRRMREILAQPALASLGARELPHSSRAQSDAQIEQFIRDWADTIYHPVGTCRMGCGP
HHHHHHHHHHHHCCHHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCC
LDVVDAQLRVHGLQALRVVDASIMPRIVSGNTNAPTVMIAERAVDLLRQAA
HHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCEEEEHHHHHHHHHHCC
>Mature Secondary Structure
MREFDYVVIGGGSAGCVLAGRLSEDPTVRVCLLEAGGSDASVLIHCPAGLAAMARSGAFN
CCCCCEEEEECCCCCEEEEECCCCCCCEEEEEEECCCCCCEEEEECCCCHHHHHHCCCCC
WGLHTTPQAGLGGRRGYQPRGKVLGGSSSVNAMIYARGHASDYDHWAAAGNAGWGWNDVL
CCCCCCCCCCCCCCCCCCCCCCEECCCCCCEEEEEECCCCCCCCHHHCCCCCCCCHHHHH
PYFLRAEHNERGASAWHGADGPLNVADLQSPQRASRAFVEAGVQAGHPRNDDFNGAQLEG
HHHHHCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEECC
VGLYQVTHRAGERFSVAKAYLTPHLGRTNLQVVTGAQATRILFAGRRATGVEYRRGGQTQ
CCHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEECCCEEEEEEECCCCCCCHHHCCCCHH
QVRATREVLLSAGALLSPQLLMLSGVGPGAQLQSHGIGVVHDLPGVGAHLHDHPDVVQVM
HHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHCCCEEEECCCCCCCCCCCCCCHHHHH
DAPRLTDLFGLSLPGALNVLRGVREWRAHRSGMLTTNFAEAGGFIKSRPEEPLPDLQLHF
CCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEECHHHHCCHHHCCCCCCCCCHHHHH
VVGKLLDHGRRATWGHGYSCHVCLLQPRSRGRVTLASADPAAAPLVDPAFFSDPDDMQRM
HHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCCCCCCCCCHHCCCHHHHHHH
VRGVRRMREILAQPALASLGARELPHSSRAQSDAQIEQFIRDWADTIYHPVGTCRMGCGP
HHHHHHHHHHHHCCHHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCC
LDVVDAQLRVHGLQALRVVDASIMPRIVSGNTNAPTVMIAERAVDLLRQAA
HHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCEEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]