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The map label for this gene is hipO [H]

Identifier: 222109664

GI number: 222109664

Start: 456908

End: 458113

Strand: Direct

Name: hipO [H]

Synonym: Dtpsy_0444

Alternate gene names: 222109664

Gene position: 456908-458113 (Clockwise)

Preceding gene: 222109663

Following gene: 222109665

Centisome position: 12.03

GC content: 67.25

Gene sequence:

>1206_bases
ATGAACATCATCGACTCCATCGCCCGCGAAGCGGCCGCCATCGCCGAGGTGCGGCGCGACATCCATGCCCATCCCGAACT
GTGCTTTCAGGAGGTGCGCACGGCCGACGTGATCGCGGCCAAGCTCACCGAATGGGGCATCCCCATCCACCGCGGGCTGG
GCACCACCGGTGTGGTGGGCATCGTGCACGGCCGTGACGGCGGTGCCTGCGGCCGCGCCGTGGGCCTGCGCGCAGACATC
GACGCCCTGCCCATGCAGGAGTTCAACACCTTCGCCCATGCGAGCAAGCACCAGGGCCGGATGCACGCCTGCGGCCACGA
CGGCCACACGGCCATGCTGTTGGCGGCGGCGCAGTATTTCTCCAAGCACCGCGACTTCGACGGCACCGTGTATTTGATCT
TCCAGCCGGCGGAAGAGGGCGGCGGCGGCGCGCGCGAGATGATCAAGGACGGTCTGTTTGAGCGGTTCCCCATGCAGGCG
GTGTTCGGCATGCACAACTGGCCGGGTATGCCGGCCGGCAGCTTTGCCGTGAGCCCCGGCCCGGTGATGGCGTCAAGCAA
TGAGTTCAAGATCACCATCCGCGGCAAGGGCAGCCACGGCGCCATGCCGCACATGGGCATCGACCCCGTGCCCGTGGCCT
GCCAGATGGTGCAGGCGTTCCAGAACATCATCAGCCGCAACAAGAAGCCCATCGAGGCGGGCGTGATCTCGGTGACGATG
ATCCACGCCGGCGAGGCCACCAACGTAGTGCCCGACAGCTGCGAGCTGCAGGGCACGGTGCGCACCTTCTCCACCGAGCT
GCTGGACATGATCGAGCGCCGCATGCGGCAGGTGGCCGAGCACACCTGCGCCGCGTTCGAGGCGCGCTGCGAATTCGAGT
TCGTGCGCAATTACCCCCCCACCATCAACTCGCCGGCCGAGGCCGAGTTCGCGCGCCAGGTGATGGTGGGCATCGTGGGT
GAAGACAAGGTGCTGGCGCAGGAGCCCACCGGGGGCGCGGAGGATTTTTCCTTCATGCTGCAGGCCAAGCCGGGCGCCTA
TGTGTTCATCGCCAACGGCGACGGCGACCACCGCGCCATGGGCCACGGCGGCGGTCCCTGCACACTGCACAACCCCAGCT
ACGACTTCAACGATGCGCTGATTCCGCTGGGCGGCACCTATTGGGTGGAACTGGCGCGCCAATGGCTGGCACAGCCCACG
GCCTGA

Upstream 100 bases:

>100_bases
CGGCGCCATCGGCGGGCCGCAGGGGCTTCGGGCCCGCTTCGGCGCGGATCGGGGAGCACGGCTAGACTGTGGCATTCCCA
GGACGACAGGCCCGATCCCC

Downstream 100 bases:

>100_bases
GAGGTTCCGGGTCCCCGTGAGGCGGCATTCACATGGCTGACATGCCCAGGCTCCACCGTTGCGCGGTGGGGCCTTTTTTA
CATGTCCAAAGGGGGTTTCG

Product: amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MNIIDSIAREAAAIAEVRRDIHAHPELCFQEVRTADVIAAKLTEWGIPIHRGLGTTGVVGIVHGRDGGACGRAVGLRADI
DALPMQEFNTFAHASKHQGRMHACGHDGHTAMLLAAAQYFSKHRDFDGTVYLIFQPAEEGGGGAREMIKDGLFERFPMQA
VFGMHNWPGMPAGSFAVSPGPVMASSNEFKITIRGKGSHGAMPHMGIDPVPVACQMVQAFQNIISRNKKPIEAGVISVTM
IHAGEATNVVPDSCELQGTVRTFSTELLDMIERRMRQVAEHTCAAFEARCEFEFVRNYPPTINSPAEAEFARQVMVGIVG
EDKVLAQEPTGGAEDFSFMLQAKPGAYVFIANGDGDHRAMGHGGGPCTLHNPSYDFNDALIPLGGTYWVELARQWLAQPT
A

Sequences:

>Translated_401_residues
MNIIDSIAREAAAIAEVRRDIHAHPELCFQEVRTADVIAAKLTEWGIPIHRGLGTTGVVGIVHGRDGGACGRAVGLRADI
DALPMQEFNTFAHASKHQGRMHACGHDGHTAMLLAAAQYFSKHRDFDGTVYLIFQPAEEGGGGAREMIKDGLFERFPMQA
VFGMHNWPGMPAGSFAVSPGPVMASSNEFKITIRGKGSHGAMPHMGIDPVPVACQMVQAFQNIISRNKKPIEAGVISVTM
IHAGEATNVVPDSCELQGTVRTFSTELLDMIERRMRQVAEHTCAAFEARCEFEFVRNYPPTINSPAEAEFARQVMVGIVG
EDKVLAQEPTGGAEDFSFMLQAKPGAYVFIANGDGDHRAMGHGGGPCTLHNPSYDFNDALIPLGGTYWVELARQWLAQPT
A
>Mature_401_residues
MNIIDSIAREAAAIAEVRRDIHAHPELCFQEVRTADVIAAKLTEWGIPIHRGLGTTGVVGIVHGRDGGACGRAVGLRADI
DALPMQEFNTFAHASKHQGRMHACGHDGHTAMLLAAAQYFSKHRDFDGTVYLIFQPAEEGGGGAREMIKDGLFERFPMQA
VFGMHNWPGMPAGSFAVSPGPVMASSNEFKITIRGKGSHGAMPHMGIDPVPVACQMVQAFQNIISRNKKPIEAGVISVTM
IHAGEATNVVPDSCELQGTVRTFSTELLDMIERRMRQVAEHTCAAFEARCEFEFVRNYPPTINSPAEAEFARQVMVGIVG
EDKVLAQEPTGGAEDFSFMLQAKPGAYVFIANGDGDHRAMGHGGGPCTLHNPSYDFNDALIPLGGTYWVELARQWLAQPT
A

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=425, Percent_Identity=26.1176470588235, Blast_Score=99, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 43378; Mature: 43378

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIIDSIAREAAAIAEVRRDIHAHPELCFQEVRTADVIAAKLTEWGIPIHRGLGTTGVVG
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCEEE
IVHGRDGGACGRAVGLRADIDALPMQEFNTFAHASKHQGRMHACGHDGHTAMLLAAAQYF
EEECCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHH
SKHRDFDGTVYLIFQPAEEGGGGAREMIKDGLFERFPMQAVFGMHNWPGMPAGSFAVSPG
HHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCEECCCC
PVMASSNEFKITIRGKGSHGAMPHMGIDPVPVACQMVQAFQNIISRNKKPIEAGVISVTM
CEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
IHAGEATNVVPDSCELQGTVRTFSTELLDMIERRMRQVAEHTCAAFEARCEFEFVRNYPP
EECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCC
TINSPAEAEFARQVMVGIVGEDKVLAQEPTGGAEDFSFMLQAKPGAYVFIANGDGDHRAM
CCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEEEECCCEEEEEECCCCCCCCC
GHGGGPCTLHNPSYDFNDALIPLGGTYWVELARQWLAQPTA
CCCCCCEEECCCCCCCCCEEEECCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNIIDSIAREAAAIAEVRRDIHAHPELCFQEVRTADVIAAKLTEWGIPIHRGLGTTGVVG
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCEEE
IVHGRDGGACGRAVGLRADIDALPMQEFNTFAHASKHQGRMHACGHDGHTAMLLAAAQYF
EEECCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHH
SKHRDFDGTVYLIFQPAEEGGGGAREMIKDGLFERFPMQAVFGMHNWPGMPAGSFAVSPG
HHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCEECCCC
PVMASSNEFKITIRGKGSHGAMPHMGIDPVPVACQMVQAFQNIISRNKKPIEAGVISVTM
CEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
IHAGEATNVVPDSCELQGTVRTFSTELLDMIERRMRQVAEHTCAAFEARCEFEFVRNYPP
EECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCC
TINSPAEAEFARQVMVGIVGEDKVLAQEPTGGAEDFSFMLQAKPGAYVFIANGDGDHRAM
CCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEEEECCCEEEEEECCCCCCCCC
GHGGGPCTLHNPSYDFNDALIPLGGTYWVELARQWLAQPTA
CCCCCCEEECCCCCCCCCEEEECCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]