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The map label for this gene is ysgA [C]

Identifier: 222109475

GI number: 222109475

Start: 272493

End: 273182

Strand: Direct

Name: ysgA [C]

Synonym: Dtpsy_0255

Alternate gene names: 222109475

Gene position: 272493-273182 (Clockwise)

Preceding gene: 222109472

Following gene: 222109482

Centisome position: 7.18

GC content: 72.9

Gene sequence:

>690_bases
ATGGGACAGTGGGTGGACCTTACCGCCAGCGACGGATTCACGGCCCCCGCCTGGGTGGCGCGCACCGACGGCGCGCCGCG
CGGCGCGGTGGTGGTGCTGCAGGAGATCTTCGGCGTCAACGCCCACATCCGCGCCGTGACCGACCGGCTGGCCATGCGCG
GCTACCTGGCGGTGGCGCCGTCCACCTTCGCGCGGGTGCAAAAGGGCGTGGACCTGGGCTACAGCGATGCCGACATGAAG
GCCGGCCGGGCACTCAAGGCCGCCGTGGAGGGGTTGCCCGCGCCGGGCGTGATGCCTGACATCCAGGCCGCCATCGACTA
TGCCGCGCGGCACAGCGACTGCAAGGTCGGCGTGGTGGGCTTTTGCTGGGGCGGGCTGCTCACCTGGCGCGCGGCTTGCA
TGCTGCAGGGGCTGTCCGCGGCCGTGCCCTACTACGGCGGCGGCATGACCACGCCCGCCGAGGTGGCGCGCCAGCCGCGC
GTGCCGGTGCTGGCGCACTTTGGCGAGCGCGACCAGTGGATCCCGCTGGACAGCGTGCAGGCCTTTGCCCGCGCGCACCC
CGAGGCGCAGGTGCATGTGTACGCGGCGGACCACGGCTTCAACTGTGACCAGCGCGCCAGCTACGACGAGGCCGCCGCCA
TGACCGCGCGCGACCGCACGCTCGCCTTCCTGGCACGGCACGTCGGCTGA

Upstream 100 bases:

>100_bases
TGACTCCTGCAAGAATGGCGCATTGGAACAGGTTTGCGGCGCTGACGCGTATCGTCCCCGCGCCTGCATTCCCTTCGCTC
AACGCCTCAGGAGACACGAC

Downstream 100 bases:

>100_bases
AATTTATAGAGAAAATCACTGCTAGCGCCAACCTGGTAAGCGCTAGCAGCTAGATTATTGATAGCGTTTTCGCATCGACA
TCGCCGCTCAATGCGCACCC

Product: carboxymethylenebutenolidase

Products: NA

Alternate protein names: Dienelactone hydrolase; DLH [H]

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MGQWVDLTASDGFTAPAWVARTDGAPRGAVVVLQEIFGVNAHIRAVTDRLAMRGYLAVAPSTFARVQKGVDLGYSDADMK
AGRALKAAVEGLPAPGVMPDIQAAIDYAARHSDCKVGVVGFCWGGLLTWRAACMLQGLSAAVPYYGGGMTTPAEVARQPR
VPVLAHFGERDQWIPLDSVQAFARAHPEAQVHVYAADHGFNCDQRASYDEAAAMTARDRTLAFLARHVG

Sequences:

>Translated_229_residues
MGQWVDLTASDGFTAPAWVARTDGAPRGAVVVLQEIFGVNAHIRAVTDRLAMRGYLAVAPSTFARVQKGVDLGYSDADMK
AGRALKAAVEGLPAPGVMPDIQAAIDYAARHSDCKVGVVGFCWGGLLTWRAACMLQGLSAAVPYYGGGMTTPAEVARQPR
VPVLAHFGERDQWIPLDSVQAFARAHPEAQVHVYAADHGFNCDQRASYDEAAAMTARDRTLAFLARHVG
>Mature_228_residues
GQWVDLTASDGFTAPAWVARTDGAPRGAVVVLQEIFGVNAHIRAVTDRLAMRGYLAVAPSTFARVQKGVDLGYSDADMKA
GRALKAAVEGLPAPGVMPDIQAAIDYAARHSDCKVGVVGFCWGGLLTWRAACMLQGLSAAVPYYGGGMTTPAEVARQPRV
PVLAHFGERDQWIPLDSVQAFARAHPEAQVHVYAADHGFNCDQRASYDEAAAMTARDRTLAFLARHVG

Specific function: Unknown

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dienelactone hydrolase family [H]

Homologues:

Organism=Escherichia coli, GI48994982, Length=238, Percent_Identity=35.2941176470588, Blast_Score=114, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: =3.1.1.45 [H]

Molecular weight: Translated: 24423; Mature: 24292

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQWVDLTASDGFTAPAWVARTDGAPRGAVVVLQEIFGVNAHIRAVTDRLAMRGYLAVAP
CCCEEEEECCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHEEECC
STFARVQKGVDLGYSDADMKAGRALKAAVEGLPAPGVMPDIQAAIDYAARHSDCKVGVVG
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEHHH
FCWGGLLTWRAACMLQGLSAAVPYYGGGMTTPAEVARQPRVPVLAHFGERDQWIPLDSVQ
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCEEECCCCCCCCCHHHHH
AFARAHPEAQVHVYAADHGFNCDQRASYDEAAAMTARDRTLAFLARHVG
HHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GQWVDLTASDGFTAPAWVARTDGAPRGAVVVLQEIFGVNAHIRAVTDRLAMRGYLAVAP
CCEEEEECCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHEEECC
STFARVQKGVDLGYSDADMKAGRALKAAVEGLPAPGVMPDIQAAIDYAARHSDCKVGVVG
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEHHH
FCWGGLLTWRAACMLQGLSAAVPYYGGGMTTPAEVARQPRVPVLAHFGERDQWIPLDSVQ
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCEEECCCCCCCCCHHHHH
AFARAHPEAQVHVYAADHGFNCDQRASYDEAAAMTARDRTLAFLARHVG
HHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]