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The map label for this gene is acsA1 [H]

Identifier: 222109299

GI number: 222109299

Start: 82917

End: 85004

Strand: Direct

Name: acsA1 [H]

Synonym: Dtpsy_0074

Alternate gene names: 222109299

Gene position: 82917-85004 (Clockwise)

Preceding gene: 222109298

Following gene: 222109305

Centisome position: 2.18

GC content: 69.4

Gene sequence:

>2088_bases
ATGGAGACACACCACAACATCCCCCCAACACACCCGCCTTACACGCCCCAGATACGCCGCTACCAGGATTGGCTGCAGGC
GCAGCACGGCCTGCGCTTTGACGATTACCACGCGCTTTGGCGCTGGTCCACCACCGATCTGGACGCCTTCTGGCAGAGCG
TCTGGGACTACTTCGACCTGCAGTCACCCACGCCGCACCGCGCGGTACTCGCAAAGAACGTGATGCCCGGCGCGCAGTGG
TTCCCCGGCGCGCAGGTGAACTACGCGCGCCAGGTGCTGCGCCATGTGGACGCCGCACAGGCCGCGGGCCAGCCGGCCCT
CATCGGCCGCAACGAACGCGGCGAGCGCCGCGAGCTGTCCTGGCCCGCGCTGCGCCGGCAGGTGGCGGCGCTGGCGCTGC
ACATGAGGGCCCAAGGCGTACAGCCCGGCGATCGCGTGGCAGCCTACCTGCCCAACATCCCCGAGACCATCGTCGCCTTC
CTGGCCTGCGCCAGCATCGGCGCGGTGTGGAGCCTGTGCGCGCCCGACATGGGCACGCACGCGGTGCTGGACCGCTTCCG
CCAGATCGCACCGACGGTGCTGTTGGCCGTGGACGGCGTCACCTATGGGGGGCGCGACCATGACCGTTGCGACGTGCTGG
CGGAACTGCGCGCGCAACTGCCCACGCTGCGCCACGTGGTGCTGGTGAGCAATCTGGACGCTTCAAAAACAGTAGCTGAT
ACCGCTTGCTACGCAAGCGTTATCGCCCGAAATGATGCAGAAACTGCGGCCTTTGAACCGGCGTGGCTGCCGTTCGACCA
CCCGCTGTGGATCGTCTATTCCAGCGGCACCACCGGCCTGCCCAAGCCCATCGTGCATGGCCATGGCGGCATGTTGCTGG
TGATGCTGCAGCTGGCGGTGCTGCACAACGACATCGGCTGCAGCTACGCGCCCAACTCCCTGGGCGAGCGCTACCACTGG
TACAGCTCCACCGGCTGGATCATGTGGAACGCCCAGGTCGGCGGCCTGCTGGGCGGCACCACCTGCGTGCTGTACGACGG
CAACCCCGGCGGCAGCAAGGAGCGGCCCGACTGGACGGTGCTGTGGCGCATGGCAGCAGAGGAGCGCGTGACCTTCTTCG
GCGCTGGCGCCGCGTTCTACGGCCACTGCATGAAGGCCGGGATCACCCCTGCGGCCTGCGGCGATCTGTCGGCGATCCGC
GTGCTGGGCTCCACGGGGTCGCCGCTGCCTGCCGAAGTGCAGCGGTGGGGATCGCAACTCCTGGCCAGCGCGGGCGTGCC
TCAGGTCTGGTGGTGCAACATCTCCGGCGGCACCGACTTCGCGGGCGCCTTCATCGGCGGCAATCGCGAGCTGCCGCAAA
CGCCCGGGCGCATGCAGTGCCGCATGCTGGGCGCAGCCGTCGAGGCCTGGGACGAGCAGGGCCGCCCGGTGCTGGACGCC
GTGGGCGAGCTGGTGTGCACCCAGCCCATCCCGTCCATGCCCTTGTTCCTGTGGGGCGACGCCGATGGCGCGCGCTACCG
CGCCAGCTACTTCGAGATGTACCCGCCCGGCCATGGCCGCAGACCCGGCGGCGGGGATCTGGGGCCCGAGGCCGGGGCCG
TCTGGCGCCACGGCGACTGGATCGAGATCGGCTCCAGCGCCCACCCCGGCTGCGTGATCTACGGCCGCAGCGACGCCACC
ATCAACCGCCACGGGCTGCGCATGGGCACGAGCGAGATCTACAGCGCCGTGGAGGCTCTGCCCGAGGTGCTCGACAGCCT
GGTCGTGGACCTGGAATACCTGGGCCGCGCCAGCTGGATGCCGCTGTTCGTCGTACTGCGCGCCGGCGCCGTGCTGGACG
AGGCCCTGCGCGCGCGCATCCAGGGCGCGATCCGCAGCCATTTGAGCCCGCGCTTCGTGCCCGACGAGATCGTGCAGGTC
GCCGAGGTGCCGCGCACGCTCAGCGGCAAGAAGCAGGAGCTGCCCATCAAGAAGCTGCTGCTGGGCCAGCCGATCGAGAA
GGTGGTCAACAAGGACGCGATGGCCAACCCGGGCTGCCTGGCGTGGTACGTGGAATTCGCGGCGTGGCGGCAGGCTCATG
CTTCGTGA

Upstream 100 bases:

>100_bases
CGGCGAGGTGCGACCGGCCAGGTGCGACCGGCCAGGTGCGACCGGCCAGTTGTCACGTGCGTGGCGCGCACGGCGACCGC
GACCGCGCTAGGCTGTGCGC

Downstream 100 bases:

>100_bases
ATGGGGGCTGCTTGTGCACGTGTGGCGAGCGTTGGCACGCATCACCGCCGAGACGAACGGCGAAGTGAAGGTCCGCTTGC
CCCTGAGCCGCTCGCTGCCG

Product: acetoacetyl-CoA synthetase

Products: NA

Alternate protein names: Acetoacetate--CoA ligase 1; Acyl-activating enzyme 1 [H]

Number of amino acids: Translated: 695; Mature: 695

Protein sequence:

>695_residues
METHHNIPPTHPPYTPQIRRYQDWLQAQHGLRFDDYHALWRWSTTDLDAFWQSVWDYFDLQSPTPHRAVLAKNVMPGAQW
FPGAQVNYARQVLRHVDAAQAAGQPALIGRNERGERRELSWPALRRQVAALALHMRAQGVQPGDRVAAYLPNIPETIVAF
LACASIGAVWSLCAPDMGTHAVLDRFRQIAPTVLLAVDGVTYGGRDHDRCDVLAELRAQLPTLRHVVLVSNLDASKTVAD
TACYASVIARNDAETAAFEPAWLPFDHPLWIVYSSGTTGLPKPIVHGHGGMLLVMLQLAVLHNDIGCSYAPNSLGERYHW
YSSTGWIMWNAQVGGLLGGTTCVLYDGNPGGSKERPDWTVLWRMAAEERVTFFGAGAAFYGHCMKAGITPAACGDLSAIR
VLGSTGSPLPAEVQRWGSQLLASAGVPQVWWCNISGGTDFAGAFIGGNRELPQTPGRMQCRMLGAAVEAWDEQGRPVLDA
VGELVCTQPIPSMPLFLWGDADGARYRASYFEMYPPGHGRRPGGGDLGPEAGAVWRHGDWIEIGSSAHPGCVIYGRSDAT
INRHGLRMGTSEIYSAVEALPEVLDSLVVDLEYLGRASWMPLFVVLRAGAVLDEALRARIQGAIRSHLSPRFVPDEIVQV
AEVPRTLSGKKQELPIKKLLLGQPIEKVVNKDAMANPGCLAWYVEFAAWRQAHAS

Sequences:

>Translated_695_residues
METHHNIPPTHPPYTPQIRRYQDWLQAQHGLRFDDYHALWRWSTTDLDAFWQSVWDYFDLQSPTPHRAVLAKNVMPGAQW
FPGAQVNYARQVLRHVDAAQAAGQPALIGRNERGERRELSWPALRRQVAALALHMRAQGVQPGDRVAAYLPNIPETIVAF
LACASIGAVWSLCAPDMGTHAVLDRFRQIAPTVLLAVDGVTYGGRDHDRCDVLAELRAQLPTLRHVVLVSNLDASKTVAD
TACYASVIARNDAETAAFEPAWLPFDHPLWIVYSSGTTGLPKPIVHGHGGMLLVMLQLAVLHNDIGCSYAPNSLGERYHW
YSSTGWIMWNAQVGGLLGGTTCVLYDGNPGGSKERPDWTVLWRMAAEERVTFFGAGAAFYGHCMKAGITPAACGDLSAIR
VLGSTGSPLPAEVQRWGSQLLASAGVPQVWWCNISGGTDFAGAFIGGNRELPQTPGRMQCRMLGAAVEAWDEQGRPVLDA
VGELVCTQPIPSMPLFLWGDADGARYRASYFEMYPPGHGRRPGGGDLGPEAGAVWRHGDWIEIGSSAHPGCVIYGRSDAT
INRHGLRMGTSEIYSAVEALPEVLDSLVVDLEYLGRASWMPLFVVLRAGAVLDEALRARIQGAIRSHLSPRFVPDEIVQV
AEVPRTLSGKKQELPIKKLLLGQPIEKVVNKDAMANPGCLAWYVEFAAWRQAHAS
>Mature_695_residues
METHHNIPPTHPPYTPQIRRYQDWLQAQHGLRFDDYHALWRWSTTDLDAFWQSVWDYFDLQSPTPHRAVLAKNVMPGAQW
FPGAQVNYARQVLRHVDAAQAAGQPALIGRNERGERRELSWPALRRQVAALALHMRAQGVQPGDRVAAYLPNIPETIVAF
LACASIGAVWSLCAPDMGTHAVLDRFRQIAPTVLLAVDGVTYGGRDHDRCDVLAELRAQLPTLRHVVLVSNLDASKTVAD
TACYASVIARNDAETAAFEPAWLPFDHPLWIVYSSGTTGLPKPIVHGHGGMLLVMLQLAVLHNDIGCSYAPNSLGERYHW
YSSTGWIMWNAQVGGLLGGTTCVLYDGNPGGSKERPDWTVLWRMAAEERVTFFGAGAAFYGHCMKAGITPAACGDLSAIR
VLGSTGSPLPAEVQRWGSQLLASAGVPQVWWCNISGGTDFAGAFIGGNRELPQTPGRMQCRMLGAAVEAWDEQGRPVLDA
VGELVCTQPIPSMPLFLWGDADGARYRASYFEMYPPGHGRRPGGGDLGPEAGAVWRHGDWIEIGSSAHPGCVIYGRSDAT
INRHGLRMGTSEIYSAVEALPEVLDSLVVDLEYLGRASWMPLFVVLRAGAVLDEALRARIQGAIRSHLSPRFVPDEIVQV
AEVPRTLSGKKQELPIKKLLLGQPIEKVVNKDAMANPGCLAWYVEFAAWRQAHAS

Specific function: Involved in poly-3-hydroxybutyrate degradation. Activates acetoacetate to acetoacetyl-CoA [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI31982927, Length=676, Percent_Identity=35.6508875739645, Blast_Score=407, Evalue=1e-113,
Organism=Homo sapiens, GI8923896, Length=696, Percent_Identity=25.5747126436782, Blast_Score=174, Evalue=3e-43,
Organism=Homo sapiens, GI28416953, Length=662, Percent_Identity=26.4350453172205, Blast_Score=171, Evalue=3e-42,
Organism=Homo sapiens, GI253970489, Length=709, Percent_Identity=24.9647390691114, Blast_Score=169, Evalue=1e-41,
Organism=Homo sapiens, GI13375727, Length=644, Percent_Identity=23.4472049689441, Blast_Score=125, Evalue=2e-28,
Organism=Escherichia coli, GI1790505, Length=672, Percent_Identity=26.4880952380952, Blast_Score=163, Evalue=3e-41,
Organism=Escherichia coli, GI1786529, Length=642, Percent_Identity=26.1682242990654, Blast_Score=137, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17569507, Length=679, Percent_Identity=32.1060382916053, Blast_Score=364, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI71982997, Length=672, Percent_Identity=25.1488095238095, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71983001, Length=672, Percent_Identity=25.1488095238095, Blast_Score=141, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6319264, Length=692, Percent_Identity=25.4335260115607, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6323182, Length=678, Percent_Identity=25.6637168141593, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24667955, Length=657, Percent_Identity=25.5707762557078, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI62472339, Length=566, Percent_Identity=26.5017667844523, Blast_Score=159, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24667959, Length=533, Percent_Identity=26.078799249531, Blast_Score=138, Evalue=1e-32,
Organism=Drosophila melanogaster, GI21355225, Length=635, Percent_Identity=22.3622047244094, Blast_Score=113, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005914
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.16 [H]

Molecular weight: Translated: 76078; Mature: 76078

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METHHNIPPTHPPYTPQIRRYQDWLQAQHGLRFDDYHALWRWSTTDLDAFWQSVWDYFDL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHEEEECCCCHHHHHHHHHHHHCC
QSPTPHRAVLAKNVMPGAQWFPGAQVNYARQVLRHVDAAQAAGQPALIGRNERGERRELS
CCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCC
WPALRRQVAALALHMRAQGVQPGDRVAAYLPNIPETIVAFLACASIGAVWSLCAPDMGTH
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
AVLDRFRQIAPTVLLAVDGVTYGGRDHDRCDVLAELRAQLPTLRHVVLVSNLDASKTVAD
HHHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHCCHHEEEEEEECCCCHHHHHH
TACYASVIARNDAETAAFEPAWLPFDHPLWIVYSSGTTGLPKPIVHGHGGMLLVMLQLAV
HHHHHHHHHCCCCCHHCCCCCCCCCCCCEEEEECCCCCCCCCCCEECCCCHHHHHHHHHH
LHNDIGCSYAPNSLGERYHWYSSTGWIMWNAQVGGLLGGTTCVLYDGNPGGSKERPDWTV
HHHCCCCCCCCHHHCCCEEEECCCCEEEEECCCCCEECCCEEEEECCCCCCCCCCCCCEE
LWRMAAEERVTFFGAGAAFYGHCMKAGITPAACGDLSAIRVLGSTGSPLPAEVQRWGSQL
EEEHHHHCCEEEEECCHHHHHHHHHHCCCCHHCCCCHHHHEECCCCCCCCHHHHHHHHHH
LASAGVPQVWWCNISGGTDFAGAFIGGNRELPQTPGRMQCRMLGAAVEAWDEQGRPVLDA
HHHCCCCEEEEEEECCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHH
VGELVCTQPIPSMPLFLWGDADGARYRASYFEMYPPGHGRRPGGGDLGPEAGAVWRHGDW
HHHHHCCCCCCCCCEEEEECCCCCCEEHHHEEECCCCCCCCCCCCCCCCCCCCEEECCCE
IEIGSSAHPGCVIYGRSDATINRHGLRMGTSEIYSAVEALPEVLDSLVVDLEYLGRASWM
EEECCCCCCCEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
PLFVVLRAGAVLDEALRARIQGAIRSHLSPRFVPDEIVQVAEVPRTLSGKKQELPIKKLL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHCCCCCCCCHHHHH
LGQPIEKVVNKDAMANPGCLAWYVEFAAWRQAHAS
HCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
METHHNIPPTHPPYTPQIRRYQDWLQAQHGLRFDDYHALWRWSTTDLDAFWQSVWDYFDL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHEEEECCCCHHHHHHHHHHHHCC
QSPTPHRAVLAKNVMPGAQWFPGAQVNYARQVLRHVDAAQAAGQPALIGRNERGERRELS
CCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCC
WPALRRQVAALALHMRAQGVQPGDRVAAYLPNIPETIVAFLACASIGAVWSLCAPDMGTH
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
AVLDRFRQIAPTVLLAVDGVTYGGRDHDRCDVLAELRAQLPTLRHVVLVSNLDASKTVAD
HHHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHCCHHEEEEEEECCCCHHHHHH
TACYASVIARNDAETAAFEPAWLPFDHPLWIVYSSGTTGLPKPIVHGHGGMLLVMLQLAV
HHHHHHHHHCCCCCHHCCCCCCCCCCCCEEEEECCCCCCCCCCCEECCCCHHHHHHHHHH
LHNDIGCSYAPNSLGERYHWYSSTGWIMWNAQVGGLLGGTTCVLYDGNPGGSKERPDWTV
HHHCCCCCCCCHHHCCCEEEECCCCEEEEECCCCCEECCCEEEEECCCCCCCCCCCCCEE
LWRMAAEERVTFFGAGAAFYGHCMKAGITPAACGDLSAIRVLGSTGSPLPAEVQRWGSQL
EEEHHHHCCEEEEECCHHHHHHHHHHCCCCHHCCCCHHHHEECCCCCCCCHHHHHHHHHH
LASAGVPQVWWCNISGGTDFAGAFIGGNRELPQTPGRMQCRMLGAAVEAWDEQGRPVLDA
HHHCCCCEEEEEEECCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHH
VGELVCTQPIPSMPLFLWGDADGARYRASYFEMYPPGHGRRPGGGDLGPEAGAVWRHGDW
HHHHHCCCCCCCCCEEEEECCCCCCEEHHHEEECCCCCCCCCCCCCCCCCCCCEEECCCE
IEIGSSAHPGCVIYGRSDATINRHGLRMGTSEIYSAVEALPEVLDSLVVDLEYLGRASWM
EEECCCCCCCEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
PLFVVLRAGAVLDEALRARIQGAIRSHLSPRFVPDEIVQVAEVPRTLSGKKQELPIKKLL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHCCCCCCCCHHHHH
LGQPIEKVVNKDAMANPGCLAWYVEFAAWRQAHAS
HCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10735852; 11481430 [H]