Definition Agrobacterium vitis S4 plasmid pAtS4e, complete sequence.
Accession NC_011981
Length 631,775

Click here to switch to the map view.

The map label for this gene is ydfG [C]

Identifier: 222102912

GI number: 222102912

Start: 597318

End: 598070

Strand: Direct

Name: ydfG [C]

Synonym: Avi_7662

Alternate gene names: 222102912

Gene position: 597318-598070 (Clockwise)

Preceding gene: 222102910

Following gene: 222102913

Centisome position: 94.55

GC content: 55.11

Gene sequence:

>753_bases
ATGCCATTTTCGGATTACAAAACTGCGCTTGTCACTGGCGCATCCTCCGGGATCGGAGCCGCTATTGTGGAGCGGCTGTG
CGCTGAGAATGTTGAAGTGCATGCGGTTGCCAGAAGTGGTGATCTCCTCAAACAGCTTTCCGAGAAAACCGGCTGCATTG
CCCATGTGATTGACGTCACGGATCGCGCCGCTCTGGCGGACCTCACATCCAAAATTCCATTTGATATTCTCGTCAATAAT
GCCGGTGTTGACCGTCCAAAGAAGTTTCTGGAAGCAGAAGACGGCGATATTGACCTGCTGGTGGATGTGAACCTGCGGGC
TGTCTTGCATCTGTGCCGCATGGTTGTGCCGGGCATGGTGGCGCGCGATCGTGGCCACGTTATCAACATCTCATCCATCG
CCGCTCAGTATAATTTCGGTGGAAATTCCTCCTATCACGCTACCAAGGCTGGCGTCAGCATGCTGTCCAACCAGTTGCGC
ATTGATGCCTTTGGCAAGCGCGTGCGGGTCACGGAAATCTGCCCCGGCCGCGTTGCAACCGATATTTTCACCCATGTCCA
CGGTGATGACCCTTCAGTGCGTGAGCGCTTTATTGATGGCTTTGAATTGCCACAGGCGTCCGACATTGCCGATGCCATTG
CCTTTGCCATTGCCGCACCCATTGCCGTTAACATCGGCCATATGGAAATCACCCCCACCCTACAAGTGATGGGCGGATTG
CAGACGGCCAAGCCGGAGGAGCGGAAGCCTTGA

Upstream 100 bases:

>100_bases
ACCAAGCTCATAAGATAGGCTTATCACTCCACATATAATCGGCATTATCGCTCAGACTAAAGCTTCGCTAGTCTTTCCCA
AGAACAAAAGAGGACGTCAG

Downstream 100 bases:

>100_bases
AGGGGTTCGATCTCTCAGCAATTCTTGGCAATCCAGACTATGTCTCCATGCTGCTGCACGGCATAGAGATGACCTTCATT
ATCGCCATTGGCTCATGGGT

Product: short-chain dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MPFSDYKTALVTGASSGIGAAIVERLCAENVEVHAVARSGDLLKQLSEKTGCIAHVIDVTDRAALADLTSKIPFDILVNN
AGVDRPKKFLEAEDGDIDLLVDVNLRAVLHLCRMVVPGMVARDRGHVINISSIAAQYNFGGNSSYHATKAGVSMLSNQLR
IDAFGKRVRVTEICPGRVATDIFTHVHGDDPSVRERFIDGFELPQASDIADAIAFAIAAPIAVNIGHMEITPTLQVMGGL
QTAKPEERKP

Sequences:

>Translated_250_residues
MPFSDYKTALVTGASSGIGAAIVERLCAENVEVHAVARSGDLLKQLSEKTGCIAHVIDVTDRAALADLTSKIPFDILVNN
AGVDRPKKFLEAEDGDIDLLVDVNLRAVLHLCRMVVPGMVARDRGHVINISSIAAQYNFGGNSSYHATKAGVSMLSNQLR
IDAFGKRVRVTEICPGRVATDIFTHVHGDDPSVRERFIDGFELPQASDIADAIAFAIAAPIAVNIGHMEITPTLQVMGGL
QTAKPEERKP
>Mature_249_residues
PFSDYKTALVTGASSGIGAAIVERLCAENVEVHAVARSGDLLKQLSEKTGCIAHVIDVTDRAALADLTSKIPFDILVNNA
GVDRPKKFLEAEDGDIDLLVDVNLRAVLHLCRMVVPGMVARDRGHVINISSIAAQYNFGGNSSYHATKAGVSMLSNQLRI
DAFGKRVRVTEICPGRVATDIFTHVHGDDPSVRERFIDGFELPQASDIADAIAFAIAAPIAVNIGHMEITPTLQVMGGLQ
TAKPEERKP

Specific function: Unknown

COG id: COG4221

COG function: function code R; Short-chain alcohol dehydrogenase of unknown specificity

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI109715829, Length=248, Percent_Identity=28.6290322580645, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI7705925, Length=245, Percent_Identity=30.6122448979592, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI7705855, Length=190, Percent_Identity=32.1052631578947, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI40807363, Length=203, Percent_Identity=27.5862068965517, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI66933014, Length=156, Percent_Identity=28.8461538461538, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI32483357, Length=224, Percent_Identity=29.4642857142857, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI20149619, Length=190, Percent_Identity=27.3684210526316, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI126723191, Length=181, Percent_Identity=32.0441988950276, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI15277342, Length=251, Percent_Identity=28.2868525896414, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI226371731, Length=191, Percent_Identity=27.2251308900524, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1787820, Length=244, Percent_Identity=34.8360655737705, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1787335, Length=247, Percent_Identity=31.5789473684211, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87082100, Length=184, Percent_Identity=34.7826086956522, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI87082160, Length=235, Percent_Identity=28.936170212766, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI2367365, Length=179, Percent_Identity=34.0782122905028, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1790717, Length=186, Percent_Identity=28.494623655914, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1787905, Length=224, Percent_Identity=30.3571428571429, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17560676, Length=226, Percent_Identity=29.2035398230088, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI71994600, Length=254, Percent_Identity=27.5590551181102, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71994604, Length=257, Percent_Identity=26.4591439688716, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI115534694, Length=249, Percent_Identity=28.5140562248996, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI193203117, Length=203, Percent_Identity=29.064039408867, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17562910, Length=212, Percent_Identity=31.1320754716981, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI193203115, Length=203, Percent_Identity=29.064039408867, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17555706, Length=250, Percent_Identity=28, Blast_Score=71, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17562904, Length=217, Percent_Identity=30.4147465437788, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17559104, Length=237, Percent_Identity=28.6919831223629, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17508895, Length=190, Percent_Identity=26.8421052631579, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25147288, Length=248, Percent_Identity=26.6129032258064, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17561402, Length=188, Percent_Identity=29.7872340425532, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17562906, Length=237, Percent_Identity=29.535864978903, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI193204405, Length=237, Percent_Identity=27.4261603375527, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17544670, Length=248, Percent_Identity=25.8064516129032, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17564282, Length=208, Percent_Identity=25.9615384615385, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6323882, Length=249, Percent_Identity=30.5220883534137, Blast_Score=86, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6322227, Length=218, Percent_Identity=27.9816513761468, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322067, Length=186, Percent_Identity=28.494623655914, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6322226, Length=191, Percent_Identity=30.3664921465969, Blast_Score=66, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24643142, Length=179, Percent_Identity=35.7541899441341, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI116007236, Length=245, Percent_Identity=30.2040816326531, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI23397609, Length=240, Percent_Identity=29.5833333333333, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24648693, Length=247, Percent_Identity=30.3643724696356, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21355889, Length=247, Percent_Identity=30.3643724696356, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24641232, Length=252, Percent_Identity=28.968253968254, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI281364494, Length=247, Percent_Identity=28.7449392712551, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24640442, Length=191, Percent_Identity=29.3193717277487, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24644339, Length=228, Percent_Identity=28.0701754385965, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24639444, Length=246, Percent_Identity=31.3008130081301, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI28571526, Length=231, Percent_Identity=28.1385281385281, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI116007234, Length=247, Percent_Identity=31.9838056680162, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24648944, Length=237, Percent_Identity=29.1139240506329, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24581726, Length=206, Percent_Identity=27.6699029126214, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21357041, Length=233, Percent_Identity=27.0386266094421, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24584752, Length=225, Percent_Identity=28, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI28571387, Length=234, Percent_Identity=26.0683760683761, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24641388, Length=255, Percent_Identity=28.6274509803922, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24649181, Length=200, Percent_Identity=28.5, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI116007238, Length=184, Percent_Identity=31.5217391304348, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21358495, Length=191, Percent_Identity=24.6073298429319, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 26795; Mature: 26664

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MPFSDYKTALVTGASSGIGAAIVERLCAENVEVHAVARSGDLLKQLSEKTGCIAHVIDVT
CCCCHHHHHEEECCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCEEEEHHHH
DRAALADLTSKIPFDILVNNAGVDRPKKFLEAEDGDIDLLVDVNLRAVLHLCRMVVPGMV
HHHHHHHHHHCCCEEEEECCCCCCCHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCHH
ARDRGHVINISSIAAQYNFGGNSSYHATKAGVSMLSNQLRIDAFGKRVRVTEICPGRVAT
CCCCCCEEEHHHHHEEECCCCCCCCHHHHHHHHHHHHHHEEECCCCEEEEEEECCCHHHH
DIFTHVHGDDPSVRERFIDGFELPQASDIADAIAFAIAAPIAVNIGHMEITPTLQVMGGL
HHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHEEECCCEEECHHHHHHCCC
QTAKPEERKP
CCCCCCCCCC
>Mature Secondary Structure 
PFSDYKTALVTGASSGIGAAIVERLCAENVEVHAVARSGDLLKQLSEKTGCIAHVIDVT
CCCHHHHHEEECCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCEEEEHHHH
DRAALADLTSKIPFDILVNNAGVDRPKKFLEAEDGDIDLLVDVNLRAVLHLCRMVVPGMV
HHHHHHHHHHCCCEEEEECCCCCCCHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCHH
ARDRGHVINISSIAAQYNFGGNSSYHATKAGVSMLSNQLRIDAFGKRVRVTEICPGRVAT
CCCCCCEEEHHHHHEEECCCCCCCCHHHHHHHHHHHHHHEEECCCCEEEEEEECCCHHHH
DIFTHVHGDDPSVRERFIDGFELPQASDIADAIAFAIAAPIAVNIGHMEITPTLQVMGGL
HHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHEEECCCEEECHHHHHHCCC
QTAKPEERKP
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]