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The map label for this gene is potH [H]

Identifier: 222102867

GI number: 222102867

Start: 544583

End: 545410

Strand: Reverse

Name: potH [H]

Synonym: Avi_7602

Alternate gene names: 222102867

Gene position: 545410-544583 (Counterclockwise)

Preceding gene: 222102868

Following gene: 222102866

Centisome position: 86.33

GC content: 57.73

Gene sequence:

>828_bases
ATGAAAGCGAGCGGTTTCCCATACGTTGTCCCGATGCTTCTGCTGTCGGTGGCGTTCTTTGCGACGCCGCTCGCCGTTCT
CGTCGGATTCAGCTTCATCGGCCCCGACGGCCTTTCTCTCCACAATTACGCACGGTTTTTTGGCGATGCATTTAACTACC
GTGTTCTGGTCAACACCGCCACGCTCGGTCTCCAGACCATTGCCAGCACGACCCTGCTCGGTGTTCCGATCGCGCTTTTC
TATTGGCACAGTGGCAAGACCGCGCGACAGGTCATCATCTTTCTGACACTTATTCCGATGCTGACCAGCAATGTGGTTCG
GACCTTTGCCTGGATCGTCATTCTTGGACGACAGGGACCGATTAGCGAGGCATTTGTCGCGCTTGGGCTCGCGGACCGCC
CGTTCACGCTGATGTCCACCGAACTCGGTCTCGTCATGGCGATGTGTCAGATCGACCTGCCTTTGATCATCTTGCCTTTG
ATTGCCATTCTCTCGCGCACGCCGGTGCAGTTCACCGAGGCGGCGCAGGTTTCAGGCGCGGGTCCATGGCGTATCTTCGT
GACGGTTCTCCTGCCAATGATGCTACCCGGTCTGCTGGCGGGCTGGATCCTTGTGTTTGCCAGCACCAGCGCGTCCTTCG
TCACCCAGGCGGTCATCGGTGGTGCGCGAAACGTCTATGTACCGCAGCTGATCTATCGAGAAGTGGGCACGCTGTTTGAT
TGGCCGATGGCCTCTGCCATCGCCGTTGTCCTCCTTCTGTCAACCGGCATCCTTCTCGTCGTCATGACCATGATTTCACG
TCACAGGAGGCTTGTCGGCCATGCATAA

Upstream 100 bases:

>100_bases
GCTGGCCACACTGGTCTACCCCGACTGGGCGGCGATCAACAAGAACCGTGCGGAATGGATCCGCCAGTTCGATGCTCTCG
TCGCCGGTTGAGGGTTTACA

Downstream 100 bases:

>100_bases
TCATCGCGAAAATCCGATCCCGACCCTTCTCTACAAGGCATTTGTTTTCGGCTTCGGCAGTCTCAGCCTGATCTATCTGG
TGGCGCCGATTATCATTGTG

Product: ABC transporter membrane spanning protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MKASGFPYVVPMLLLSVAFFATPLAVLVGFSFIGPDGLSLHNYARFFGDAFNYRVLVNTATLGLQTIASTTLLGVPIALF
YWHSGKTARQVIIFLTLIPMLTSNVVRTFAWIVILGRQGPISEAFVALGLADRPFTLMSTELGLVMAMCQIDLPLIILPL
IAILSRTPVQFTEAAQVSGAGPWRIFVTVLLPMMLPGLLAGWILVFASTSASFVTQAVIGGARNVYVPQLIYREVGTLFD
WPMASAIAVVLLLSTGILLVVMTMISRHRRLVGHA

Sequences:

>Translated_275_residues
MKASGFPYVVPMLLLSVAFFATPLAVLVGFSFIGPDGLSLHNYARFFGDAFNYRVLVNTATLGLQTIASTTLLGVPIALF
YWHSGKTARQVIIFLTLIPMLTSNVVRTFAWIVILGRQGPISEAFVALGLADRPFTLMSTELGLVMAMCQIDLPLIILPL
IAILSRTPVQFTEAAQVSGAGPWRIFVTVLLPMMLPGLLAGWILVFASTSASFVTQAVIGGARNVYVPQLIYREVGTLFD
WPMASAIAVVLLLSTGILLVVMTMISRHRRLVGHA
>Mature_275_residues
MKASGFPYVVPMLLLSVAFFATPLAVLVGFSFIGPDGLSLHNYARFFGDAFNYRVLVNTATLGLQTIASTTLLGVPIALF
YWHSGKTARQVIIFLTLIPMLTSNVVRTFAWIVILGRQGPISEAFVALGLADRPFTLMSTELGLVMAMCQIDLPLIILPL
IAILSRTPVQFTEAAQVSGAGPWRIFVTVLLPMMLPGLLAGWILVFASTSASFVTQAVIGGARNVYVPQLIYREVGTLFD
WPMASAIAVVLLLSTGILLVVMTMISRHRRLVGHA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=233, Percent_Identity=28.3261802575107, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1787369, Length=258, Percent_Identity=27.1317829457364, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787713, Length=227, Percent_Identity=29.0748898678414, Blast_Score=85, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29782; Mature: 29782

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASGFPYVVPMLLLSVAFFATPLAVLVGFSFIGPDGLSLHNYARFFGDAFNYRVLVNTA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEEHH
TLGLQTIASTTLLGVPIALFYWHSGKTARQVIIFLTLIPMLTSNVVRTFAWIVILGRQGP
HHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ISEAFVALGLADRPFTLMSTELGLVMAMCQIDLPLIILPLIAILSRTPVQFTEAAQVSGA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHCCCC
GPWRIFVTVLLPMMLPGLLAGWILVFASTSASFVTQAVIGGARNVYVPQLIYREVGTLFD
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
WPMASAIAVVLLLSTGILLVVMTMISRHRRLVGHA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKASGFPYVVPMLLLSVAFFATPLAVLVGFSFIGPDGLSLHNYARFFGDAFNYRVLVNTA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEEHH
TLGLQTIASTTLLGVPIALFYWHSGKTARQVIIFLTLIPMLTSNVVRTFAWIVILGRQGP
HHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ISEAFVALGLADRPFTLMSTELGLVMAMCQIDLPLIILPLIAILSRTPVQFTEAAQVSGA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHCCCC
GPWRIFVTVLLPMMLPGLLAGWILVFASTSASFVTQAVIGGARNVYVPQLIYREVGTLFD
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
WPMASAIAVVLLLSTGILLVVMTMISRHRRLVGHA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]