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The map label for this gene is orfB [C]

Identifier: 222102790

GI number: 222102790

Start: 454438

End: 455295

Strand: Reverse

Name: orfB [C]

Synonym: Avi_7504

Alternate gene names: 222102790

Gene position: 455295-454438 (Counterclockwise)

Preceding gene: 222102791

Following gene: 222102789

Centisome position: 72.07

GC content: 58.28

Gene sequence:

>858_bases
ATGAAACGGGACATCCTCATGACAAACGCGGCTCAACCATCCGTAAAATCCACCCCCTACTGGTGGGATGGCTGCACGTT
TCCAACCTTGTCGACCAAGCCGGTGAGCGCCACCTGTGACGTCGCTATTGTTGGCGGTGGCTACACCGGTCTCTCAGCCG
CCATCGAGCTTGCGCGGGCCGGCCTGCATGTTCAGCTGTTTGATCGGACATCGCTTGGGCAAGCCGCATCCAGCCGCAAC
GGCTGGGTGGTGAATCCGAAGAGAAACTATCGTCTTTACAAGGAGATGGACCTGCAACTGCGCAACAAGGTGCCAAAACG
GCGCGTCAAAGCAAAGCTGCGATCGGACCGCACCGCAGCCACCCGTTCGAACGACGTCTGGGCCATGGATTTCGTGCATG
ACCAACTGGCCACAGGTCGCAAGATCAGGGTTCTCACGGTTGTCGATACCTTCTCGCGCTTCTCTCCGGCGGTAGATGCC
CGCTTCAGCTATAAAGGGGAAGACGTCGTGCAGACCCTCGAACGCGTATGCCGGCAGGTCGGTTACCCGGCCACCATTCG
GGTGGACAACGGCAGCGAATTCATCTCTCGTGACCTCGATCTCTGGGCCTATCATAGAGGCGTCGTGCTTGACTTCTCGC
GGCCGGGCAAGCCGACGGACAACAGCTACATCGAGAGTTTTAATGGCAAGTTCCGGGCAGAGTGCCTGAACGCCCACTGG
TTTATGAGCCTTGACGACGCAAGGGCAAAGATGGAGGATTGGCGTAGAGACTATAACGAGTTCCGGCCACACAGCGCGAT
CGGCAACAAGGTGCCGATTTCGCTTATGAACGGCTCATCGGCACCCACGCCAACCTGA

Upstream 100 bases:

>100_bases
ATGCATCCGGGCTTTACCGGGGTTTGGAAGGTCATCGAAACCACGCGGAAACTCTGGGTCTCTCGCGATTGAGATTGTCT
CACGCATTCCTTCCGATCAT

Downstream 100 bases:

>100_bases
GCCTTAACCCCGGAAAATTCCAGCTCCCGCTGGCCCAAAACCGGGGTGCACTTCAGAGGCGAGTGCCAACAGGCGTCGTA
CCTGGGCTGCATCCTTCGTC

Product: IS3 family transposase

Products: NA

Alternate protein names: ORF1 [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MKRDILMTNAAQPSVKSTPYWWDGCTFPTLSTKPVSATCDVAIVGGGYTGLSAAIELARAGLHVQLFDRTSLGQAASSRN
GWVVNPKRNYRLYKEMDLQLRNKVPKRRVKAKLRSDRTAATRSNDVWAMDFVHDQLATGRKIRVLTVVDTFSRFSPAVDA
RFSYKGEDVVQTLERVCRQVGYPATIRVDNGSEFISRDLDLWAYHRGVVLDFSRPGKPTDNSYIESFNGKFRAECLNAHW
FMSLDDARAKMEDWRRDYNEFRPHSAIGNKVPISLMNGSSAPTPT

Sequences:

>Translated_285_residues
MKRDILMTNAAQPSVKSTPYWWDGCTFPTLSTKPVSATCDVAIVGGGYTGLSAAIELARAGLHVQLFDRTSLGQAASSRN
GWVVNPKRNYRLYKEMDLQLRNKVPKRRVKAKLRSDRTAATRSNDVWAMDFVHDQLATGRKIRVLTVVDTFSRFSPAVDA
RFSYKGEDVVQTLERVCRQVGYPATIRVDNGSEFISRDLDLWAYHRGVVLDFSRPGKPTDNSYIESFNGKFRAECLNAHW
FMSLDDARAKMEDWRRDYNEFRPHSAIGNKVPISLMNGSSAPTPT
>Mature_285_residues
MKRDILMTNAAQPSVKSTPYWWDGCTFPTLSTKPVSATCDVAIVGGGYTGLSAAIELARAGLHVQLFDRTSLGQAASSRN
GWVVNPKRNYRLYKEMDLQLRNKVPKRRVKAKLRSDRTAATRSNDVWAMDFVHDQLATGRKIRVLTVVDTFSRFSPAVDA
RFSYKGEDVVQTLERVCRQVGYPATIRVDNGSEFISRDLDLWAYHRGVVLDFSRPGKPTDNSYIESFNGKFRAECLNAHW
FMSLDDARAKMEDWRRDYNEFRPHSAIGNKVPISLMNGSSAPTPT

Specific function: Unknown

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1786462, Length=156, Percent_Identity=32.0512820512821, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 32163; Mature: 32163

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRDILMTNAAQPSVKSTPYWWDGCTFPTLSTKPVSATCDVAIVGGGYTGLSAAIELARA
CCCCEEEECCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHC
GLHVQLFDRTSLGQAASSRNGWVVNPKRNYRLYKEMDLQLRNKVPKRRVKAKLRSDRTAA
CCEEEEECCCCCCCHHCCCCCEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
TRSNDVWAMDFVHDQLATGRKIRVLTVVDTFSRFSPAVDARFSYKGEDVVQTLERVCRQV
CCCCCEEEEHHHHHHHHCCCEEEEEEEEHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHC
GYPATIRVDNGSEFISRDLDLWAYHRGVVLDFSRPGKPTDNSYIESFNGKFRAECLNAHW
CCCEEEEECCCHHHHHCCCHHEEECCCEEEECCCCCCCCCCHHHHHCCCCEEEEEECCEE
FMSLDDARAKMEDWRRDYNEFRPHSAIGNKVPISLMNGSSAPTPT
EEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCC
>Mature Secondary Structure
MKRDILMTNAAQPSVKSTPYWWDGCTFPTLSTKPVSATCDVAIVGGGYTGLSAAIELARA
CCCCEEEECCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHC
GLHVQLFDRTSLGQAASSRNGWVVNPKRNYRLYKEMDLQLRNKVPKRRVKAKLRSDRTAA
CCEEEEECCCCCCCHHCCCCCEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
TRSNDVWAMDFVHDQLATGRKIRVLTVVDTFSRFSPAVDARFSYKGEDVVQTLERVCRQV
CCCCCEEEEHHHHHHHHCCCEEEEEEEEHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHC
GYPATIRVDNGSEFISRDLDLWAYHRGVVLDFSRPGKPTDNSYIESFNGKFRAECLNAHW
CCCEEEEECCCHHHHHCCCHHEEECCCEEEECCCCCCCCCCHHHHHCCCCEEEEEECCEE
FMSLDDARAKMEDWRRDYNEFRPHSAIGNKVPISLMNGSSAPTPT
EEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1718819 [H]