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The map label for this gene is nocT [H]

Identifier: 222102594

GI number: 222102594

Start: 201032

End: 201883

Strand: Direct

Name: nocT [H]

Synonym: Avi_7239

Alternate gene names: 222102594

Gene position: 201032-201883 (Clockwise)

Preceding gene: 222102593

Following gene: 222102595

Centisome position: 31.82

GC content: 55.05

Gene sequence:

>852_bases
ATGACGTTTCGTCGCATGGCCGCGTTGGCGGTCGCCTTCACGACACTGATATCGGGCTCAGTCACCATGGCGCAAGACGC
GCCGACCGAGATCAAGATTGCAACCGAAGGAGCCTATGCTCCCTGGAACTTTACGACGTCCGACGGCAAGCTCGATGGCC
TTGAAATCGAACTCGCCAATGACCTCTGCGCACGCATGAAGGTCAAATGCACCATCGTTGCTCAGGATTGGGACGGTCTT
ATTCCGTCGCTGACGGTTGGCAAGTTCGATGTCATTATGGCGAGCATGTTCATCACGCCAAAGCGTCTTGAGACGATCGA
TTTTACCCAGCCTTATGCGGTGGATCCTTCGGGTTTTGCTGTTCCCAAGGATAGCGCGGTTGGCAAGCTCGGCATCTCGA
CAGAAAAATTCAAACTTGAGGATGAGGCCCCCTCGAAAGCGGCGATCGACAAGTTGAAATCCCTCCTGAAGGGCAAAGTT
GTTGGCGTTCAGGCCGCAACGACAAATCTCGATTTTCTGCGTAAGTATTTTGCCGATACCGTAGAAATCCGCGAGTACAA
AACCACAGAGCAGCATGACCTCGACTTGGCAGCTGGTCGCATTGATGCGATCTTCGCCCAGCAGACGGCACTGGCCGCGA
CACTCGCAAAGCCAGAATTCGCAGACTATACCATTGCAGGCCCCGGCTTCGTGGGCGGCGTCTTCGGCCTTGGAACCGGT
GCGGGATTGCGCAAGCAGGACACCAAGCTCAAGGAGATGCTGAACACCGCAATCTCCTCGGCTATTGCCGACGGGACCAT
CAAACGTCTCTCCGAAAAATGGGTGAAAACCGACGTGACACCGGTCAAGTAA

Upstream 100 bases:

>100_bases
CGGCGCTGAACGCTGATCGGTCACTATGTCCGAAGCCGGAGGCAACATCCGGCAAGGATGGGGAACCGCCGTAAAAACGG
CTCAAAAAAGAGGAAAAGTC

Downstream 100 bases:

>100_bases
CAGAGGTGCGGACCGCATGCGGCCAGGGCCTTGGTTGCACTGGCCGCGTGAACTGCGAGAGGGTTTCGCCCATATGCTTG
AAAAACTGCATCTGCTCGGG

Product: ABC transporter substrate binding protein (nopaline)

Products: ADP; phosphate; L-histidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MTFRRMAALAVAFTTLISGSVTMAQDAPTEIKIATEGAYAPWNFTTSDGKLDGLEIELANDLCARMKVKCTIVAQDWDGL
IPSLTVGKFDVIMASMFITPKRLETIDFTQPYAVDPSGFAVPKDSAVGKLGISTEKFKLEDEAPSKAAIDKLKSLLKGKV
VGVQAATTNLDFLRKYFADTVEIREYKTTEQHDLDLAAGRIDAIFAQQTALAATLAKPEFADYTIAGPGFVGGVFGLGTG
AGLRKQDTKLKEMLNTAISSAIADGTIKRLSEKWVKTDVTPVK

Sequences:

>Translated_283_residues
MTFRRMAALAVAFTTLISGSVTMAQDAPTEIKIATEGAYAPWNFTTSDGKLDGLEIELANDLCARMKVKCTIVAQDWDGL
IPSLTVGKFDVIMASMFITPKRLETIDFTQPYAVDPSGFAVPKDSAVGKLGISTEKFKLEDEAPSKAAIDKLKSLLKGKV
VGVQAATTNLDFLRKYFADTVEIREYKTTEQHDLDLAAGRIDAIFAQQTALAATLAKPEFADYTIAGPGFVGGVFGLGTG
AGLRKQDTKLKEMLNTAISSAIADGTIKRLSEKWVKTDVTPVK
>Mature_282_residues
TFRRMAALAVAFTTLISGSVTMAQDAPTEIKIATEGAYAPWNFTTSDGKLDGLEIELANDLCARMKVKCTIVAQDWDGLI
PSLTVGKFDVIMASMFITPKRLETIDFTQPYAVDPSGFAVPKDSAVGKLGISTEKFKLEDEAPSKAAIDKLKSLLKGKVV
GVQAATTNLDFLRKYFADTVEIREYKTTEQHDLDLAAGRIDAIFAQQTALAATLAKPEFADYTIAGPGFVGGVFGLGTGA
GLRKQDTKLKEMLNTAISSAIADGTIKRLSEKWVKTDVTPVK

Specific function: Component of the octopine active transport system probably consisting of four subunits:Q, M, P and T [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788648, Length=256, Percent_Identity=39.0625, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1788649, Length=275, Percent_Identity=32, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787088, Length=267, Percent_Identity=30.3370786516854, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1787085, Length=260, Percent_Identity=28.0769230769231, Blast_Score=110, Evalue=7e-26,
Organism=Escherichia coli, GI1788228, Length=248, Percent_Identity=33.4677419354839, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787031, Length=275, Percent_Identity=22.5454545454545, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 3400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 30469; Mature: 30338

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFRRMAALAVAFTTLISGSVTMAQDAPTEIKIATEGAYAPWNFTTSDGKLDGLEIELAN
CCHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCCCCCEEECCCCCCCCEEEEEHH
DLCARMKVKCTIVAQDWDGLIPSLTVGKFDVIMASMFITPKRLETIDFTQPYAVDPSGFA
HHHHCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCHHCCEECCCCCCEECCCCCC
VPKDSAVGKLGISTEKFKLEDEAPSKAAIDKLKSLLKGKVVGVQAATTNLDFLRKYFADT
CCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHH
VEIREYKTTEQHDLDLAAGRIDAIFAQQTALAATLAKPEFADYTIAGPGFVGGVFGLGTG
HHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHCCCC
AGLRKQDTKLKEMLNTAISSAIADGTIKRLSEKWVKTDVTPVK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TFRRMAALAVAFTTLISGSVTMAQDAPTEIKIATEGAYAPWNFTTSDGKLDGLEIELAN
CHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCCCCCEEECCCCCCCCEEEEEHH
DLCARMKVKCTIVAQDWDGLIPSLTVGKFDVIMASMFITPKRLETIDFTQPYAVDPSGFA
HHHHCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCHHCCEECCCCCCEECCCCCC
VPKDSAVGKLGISTEKFKLEDEAPSKAAIDKLKSLLKGKVVGVQAATTNLDFLRKYFADT
CCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHH
VEIREYKTTEQHDLDLAAGRIDAIFAQQTALAATLAKPEFADYTIAGPGFVGGVFGLGTG
HHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHCCCC
AGLRKQDTKLKEMLNTAISSAIADGTIKRLSEKWVKTDVTPVK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA