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The map label for this gene is topB [H]

Identifier: 222094156

GI number: 222094156

Start: 445990

End: 448179

Strand: Direct

Name: topB [H]

Synonym: BCQ_0449

Alternate gene names: 222094156

Gene position: 445990-448179 (Clockwise)

Preceding gene: 222094155

Following gene: 222094157

Centisome position: 8.55

GC content: 35.84

Gene sequence:

>2190_bases
ATGGCAAAGAGTGTAGTAATTGCTGAGAAACCATCAGTTGCACGTGATATAGCAAAAGTATTAAAGTGTACAAAAAAAGG
TAATGGATTTTTAGAAGGTGACAAATACATCGTGACTTGGGCTCTTGGGCATTTAGTGACGCTTGCAGATCCTGAGACTT
ATGATAATAAGTATAAAACATGGACTCTTGAAGATCTTCCAATGCTTCCAGAACGTATGAAATTAGTTGTTATAAAACAA
ACGGGAAAACAGTTTAATGCTGTGAAACATCAACTTACTCGAAATGATGTTAATGAGCTGATTGTGGCTACCGATGCAGG
AAGAGAGGGCGAATTAGTCGCACGTTGGATAATTGAAAAAGCAAAAGTAAATAAACCAATTAAGCGTTTATGGATTTCTT
CTGTAACAGACAAAGCAATTAAAGATGGTTTTAATAATTTAAAACCAGGTAAAGCTTATGACAATTTATATGCCTCAGCC
GTTGCCCGCTCTGAAGCAGATTGGTATATTGGCTTAAATGCTACCCGTGCATTAACAACAAAATTCAATGCTCAATTAAA
CTGTGGGCGTGTACAAACTCCAACTGTAGCTATTATTGCTGGTCGTGAGGATGAAATTAAAAACTTTAAGGCTCAAACAT
ACTACGGTATCGAAGCGCAAACAAACGATAGGTTAAAACTAACTTGGCAAGATGCAAATGGTAATAGCCGTAGTTTTAAT
AAAGAAAAAATCGATGATATTGTAAAAAATCTAGATAAACAAAATGCCACTGTTGTGGAAATTGATAAAAAGCAGAAGAA
GTCATTCTCTCCTGGTCTTTACGATTTAACTGAATTGCAACGTGATGCCAATAAAAAGTTTGGTTACTCTGCGAAAGAAA
CATTAAACATTATGCAGAAATTATATGAACAACATAAAGTGCTAACGTATCCTCGTACAGATTCACGCTACATTTCATCT
GATATCGTTGGAACACTTCCAGAGCGTCTAAAAGCATGTGGTGTTGGAGAATACCGTCCTTTAGCACATAAAGTATTACA
AAAGCCTATCAAACCTAATAAATCATTTGTTGATGATAGTAAAGTGAGCGATCATCATGCGATTATTCCAACAGAAGGAT
ACGTTAACTTTTCAGCATTCACAGATAAAGAACGCAAAATTTATGATTTAGTTGTAAAACGTTTCTTAGCTGTTTTATTC
CCAGCATTCGAATACGAGCAACTAACATTACGTACCAAAGTTGGTAACGAAACGTTCATTGCACGCGGAAAAACGATTTT
ACATGCTGGTTGGAAGGAAGTATATGAAAATCGATTTGAGGATGATGATGTAACTGATGACGTAAAAGAGCAGCTTTTAC
CTCGTATCGAAAAAGGCGATACATTAACTGTAAAGTTAATTATGCAAACATCAGGCCAAACGAAAGCACCTGCACGTTTT
AACGAAGCGACTTTACTTTCAGCAATGGAAAATCCTACGAAATATATGGATACGCAAAATAAACAACTTGCTGATACGTT
AAAATCAACTGGTGGATTAGGTACTGTAGCAACGCGTGCTGATATTATCGACAAGCTATTCAATTCATTTTTAATTGAAA
AACGTGGTAAAGATATTCACATTACTTCAAAAGGTCGTCAATTACTTGATTTAGTACCAGAAGAGTTAAAATCACCTACA
CTAACTGGTGAGTGGGAACAAAAGCTAGAGGCAATTGCAAAGGGGAAACTGAAAAAAGAAGTATTCATTTCCGAAATGAA
GAACTATACGAAAGAAATTGTTTCTGAAATTAAATCGAGTGATAAAAAATATAAACATGACAACATTTCAACGAAGTCTT
GCCCGGACTGCGGTAAACCAATGTTAGAAGTAAACGGCAAAAAAGGAAAAATGCTCGTATGCCAAGACCGTGAATGCGGT
CATCGTAAAAATGTATCTCGTACAACAAACGCTCGTTGCCCACAATGTAAGAAGAAACTAGAATTACGTGGTGAAGGTGC
AGGTCAAATCTTTGCATGTAAATGTGGCTATCGCGAGAAGTTATCGACATTCCAAGAGCGACGTAAAAAAGAATCTGGAA
ACAAAGCGGATAAACGCGACGTTCAAAAGTATATGAAACAACAGAAAAAAGAAGAAGAACCATTAAATAATGCATTCGCA
GAAGCATTAAAGAAATTAAAATTTGATTAA

Upstream 100 bases:

>100_bases
GTCATGTCAATTATGACTGATATATAAGGTGCTTCTTACTGCTCTTCTTGAAAACTGTGGTATATTAAACGGTATATAGT
TAGTAAAGGAGTTTGAATAA

Downstream 100 bases:

>100_bases
AATGAGATCATAAAATTTAATTTAGCAGTTAGTTTAAAAACTCCATTTGGAATAAAGGTTATTCCAAATGGAGTTAATTC
TTTAAATTATTTTTTTGATC

Product: DNA topoisomerase iii

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 729; Mature: 728

Protein sequence:

>729_residues
MAKSVVIAEKPSVARDIAKVLKCTKKGNGFLEGDKYIVTWALGHLVTLADPETYDNKYKTWTLEDLPMLPERMKLVVIKQ
TGKQFNAVKHQLTRNDVNELIVATDAGREGELVARWIIEKAKVNKPIKRLWISSVTDKAIKDGFNNLKPGKAYDNLYASA
VARSEADWYIGLNATRALTTKFNAQLNCGRVQTPTVAIIAGREDEIKNFKAQTYYGIEAQTNDRLKLTWQDANGNSRSFN
KEKIDDIVKNLDKQNATVVEIDKKQKKSFSPGLYDLTELQRDANKKFGYSAKETLNIMQKLYEQHKVLTYPRTDSRYISS
DIVGTLPERLKACGVGEYRPLAHKVLQKPIKPNKSFVDDSKVSDHHAIIPTEGYVNFSAFTDKERKIYDLVVKRFLAVLF
PAFEYEQLTLRTKVGNETFIARGKTILHAGWKEVYENRFEDDDVTDDVKEQLLPRIEKGDTLTVKLIMQTSGQTKAPARF
NEATLLSAMENPTKYMDTQNKQLADTLKSTGGLGTVATRADIIDKLFNSFLIEKRGKDIHITSKGRQLLDLVPEELKSPT
LTGEWEQKLEAIAKGKLKKEVFISEMKNYTKEIVSEIKSSDKKYKHDNISTKSCPDCGKPMLEVNGKKGKMLVCQDRECG
HRKNVSRTTNARCPQCKKKLELRGEGAGQIFACKCGYREKLSTFQERRKKESGNKADKRDVQKYMKQQKKEEEPLNNAFA
EALKKLKFD

Sequences:

>Translated_729_residues
MAKSVVIAEKPSVARDIAKVLKCTKKGNGFLEGDKYIVTWALGHLVTLADPETYDNKYKTWTLEDLPMLPERMKLVVIKQ
TGKQFNAVKHQLTRNDVNELIVATDAGREGELVARWIIEKAKVNKPIKRLWISSVTDKAIKDGFNNLKPGKAYDNLYASA
VARSEADWYIGLNATRALTTKFNAQLNCGRVQTPTVAIIAGREDEIKNFKAQTYYGIEAQTNDRLKLTWQDANGNSRSFN
KEKIDDIVKNLDKQNATVVEIDKKQKKSFSPGLYDLTELQRDANKKFGYSAKETLNIMQKLYEQHKVLTYPRTDSRYISS
DIVGTLPERLKACGVGEYRPLAHKVLQKPIKPNKSFVDDSKVSDHHAIIPTEGYVNFSAFTDKERKIYDLVVKRFLAVLF
PAFEYEQLTLRTKVGNETFIARGKTILHAGWKEVYENRFEDDDVTDDVKEQLLPRIEKGDTLTVKLIMQTSGQTKAPARF
NEATLLSAMENPTKYMDTQNKQLADTLKSTGGLGTVATRADIIDKLFNSFLIEKRGKDIHITSKGRQLLDLVPEELKSPT
LTGEWEQKLEAIAKGKLKKEVFISEMKNYTKEIVSEIKSSDKKYKHDNISTKSCPDCGKPMLEVNGKKGKMLVCQDRECG
HRKNVSRTTNARCPQCKKKLELRGEGAGQIFACKCGYREKLSTFQERRKKESGNKADKRDVQKYMKQQKKEEEPLNNAFA
EALKKLKFD
>Mature_728_residues
AKSVVIAEKPSVARDIAKVLKCTKKGNGFLEGDKYIVTWALGHLVTLADPETYDNKYKTWTLEDLPMLPERMKLVVIKQT
GKQFNAVKHQLTRNDVNELIVATDAGREGELVARWIIEKAKVNKPIKRLWISSVTDKAIKDGFNNLKPGKAYDNLYASAV
ARSEADWYIGLNATRALTTKFNAQLNCGRVQTPTVAIIAGREDEIKNFKAQTYYGIEAQTNDRLKLTWQDANGNSRSFNK
EKIDDIVKNLDKQNATVVEIDKKQKKSFSPGLYDLTELQRDANKKFGYSAKETLNIMQKLYEQHKVLTYPRTDSRYISSD
IVGTLPERLKACGVGEYRPLAHKVLQKPIKPNKSFVDDSKVSDHHAIIPTEGYVNFSAFTDKERKIYDLVVKRFLAVLFP
AFEYEQLTLRTKVGNETFIARGKTILHAGWKEVYENRFEDDDVTDDVKEQLLPRIEKGDTLTVKLIMQTSGQTKAPARFN
EATLLSAMENPTKYMDTQNKQLADTLKSTGGLGTVATRADIIDKLFNSFLIEKRGKDIHITSKGRQLLDLVPEELKSPTL
TGEWEQKLEAIAKGKLKKEVFISEMKNYTKEIVSEIKSSDKKYKHDNISTKSCPDCGKPMLEVNGKKGKMLVCQDRECGH
RKNVSRTTNARCPQCKKKLELRGEGAGQIFACKCGYREKLSTFQERRKKESGNKADKRDVQKYMKQQKKEEEPLNNAFAE
ALKKLKFD

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=622, Percent_Identity=25.7234726688103, Blast_Score=136, Evalue=8e-32,
Organism=Homo sapiens, GI10835218, Length=643, Percent_Identity=24.5723172628305, Blast_Score=134, Evalue=4e-31,
Organism=Escherichia coli, GI1788061, Length=620, Percent_Identity=32.4193548387097, Blast_Score=339, Evalue=4e-94,
Organism=Escherichia coli, GI1787529, Length=671, Percent_Identity=25.3353204172876, Blast_Score=160, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI32563869, Length=747, Percent_Identity=25.0334672021419, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17555378, Length=642, Percent_Identity=24.7663551401869, Blast_Score=124, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6323263, Length=699, Percent_Identity=22.1745350500715, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24585251, Length=593, Percent_Identity=25.4637436762226, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24640096, Length=619, Percent_Identity=24.232633279483, Blast_Score=114, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 82830; Mature: 82699

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSVVIAEKPSVARDIAKVLKCTKKGNGFLEGDKYIVTWALGHLVTLADPETYDNKYKT
CCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHEEEECCCCCCCCCEEE
WTLEDLPMLPERMKLVVIKQTGKQFNAVKHQLTRNDVNELIVATDAGREGELVARWIIEK
EECCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCHHHEEEEECCCCCCHHHHHHHHHH
AKVNKPIKRLWISSVTDKAIKDGFNNLKPGKAYDNLYASAVARSEADWYIGLNATRALTT
HHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHE
KFNAQLNCGRVQTPTVAIIAGREDEIKNFKAQTYYGIEAQTNDRLKLTWQDANGNSRSFN
ECCCEECCCCCCCCEEEEEECCHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCC
KEKIDDIVKNLDKQNATVVEIDKKQKKSFSPGLYDLTELQRDANKKFGYSAKETLNIMQK
HHHHHHHHHHHCCCCCEEEEECCHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHH
LYEQHKVLTYPRTDSRYISSDIVGTLPERLKACGVGEYRPLAHKVLQKPIKPNKSFVDDS
HHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCC
KVSDHHAIIPTEGYVNFSAFTDKERKIYDLVVKRFLAVLFPAFEYEQLTLRTKVGNETFI
CCCCCCEEECCCCCEEEHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEE
ARGKTILHAGWKEVYENRFEDDDVTDDVKEQLLPRIEKGDTLTVKLIMQTSGQTKAPARF
ECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCC
NEATLLSAMENPTKYMDTQNKQLADTLKSTGGLGTVATRADIIDKLFNSFLIEKRGKDIH
CHHHHHHHHCCCHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
ITSKGRQLLDLVPEELKSPTLTGEWEQKLEAIAKGKLKKEVFISEMKNYTKEIVSEIKSS
EECCCHHHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DKKYKHDNISTKSCPDCGKPMLEVNGKKGKMLVCQDRECGHRKNVSRTTNARCPQCKKKL
CCHHCCCCCCCCCCCCCCCCCEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHH
ELRGEGAGQIFACKCGYREKLSTFQERRKKESGNKADKRDVQKYMKQQKKEEEPLNNAFA
HCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHH
EALKKLKFD
HHHHHHCCC
>Mature Secondary Structure 
AKSVVIAEKPSVARDIAKVLKCTKKGNGFLEGDKYIVTWALGHLVTLADPETYDNKYKT
CCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHEEEECCCCCCCCCEEE
WTLEDLPMLPERMKLVVIKQTGKQFNAVKHQLTRNDVNELIVATDAGREGELVARWIIEK
EECCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCHHHEEEEECCCCCCHHHHHHHHHH
AKVNKPIKRLWISSVTDKAIKDGFNNLKPGKAYDNLYASAVARSEADWYIGLNATRALTT
HHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHE
KFNAQLNCGRVQTPTVAIIAGREDEIKNFKAQTYYGIEAQTNDRLKLTWQDANGNSRSFN
ECCCEECCCCCCCCEEEEEECCHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCC
KEKIDDIVKNLDKQNATVVEIDKKQKKSFSPGLYDLTELQRDANKKFGYSAKETLNIMQK
HHHHHHHHHHHCCCCCEEEEECCHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHH
LYEQHKVLTYPRTDSRYISSDIVGTLPERLKACGVGEYRPLAHKVLQKPIKPNKSFVDDS
HHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCC
KVSDHHAIIPTEGYVNFSAFTDKERKIYDLVVKRFLAVLFPAFEYEQLTLRTKVGNETFI
CCCCCCEEECCCCCEEEHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEE
ARGKTILHAGWKEVYENRFEDDDVTDDVKEQLLPRIEKGDTLTVKLIMQTSGQTKAPARF
ECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCC
NEATLLSAMENPTKYMDTQNKQLADTLKSTGGLGTVATRADIIDKLFNSFLIEKRGKDIH
CHHHHHHHHCCCHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
ITSKGRQLLDLVPEELKSPTLTGEWEQKLEAIAKGKLKKEVFISEMKNYTKEIVSEIKSS
EECCCHHHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DKKYKHDNISTKSCPDCGKPMLEVNGKKGKMLVCQDRECGHRKNVSRTTNARCPQCKKKL
CCHHCCCCCCCCCCCCCCCCCEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHH
ELRGEGAGQIFACKCGYREKLSTFQERRKKESGNKADKRDVQKYMKQQKKEEEPLNNAFA
HCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHH
EALKKLKFD
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA