Click here to switch to the map view.

The map label for this gene is pleD [H]

Identifier: 221635980

GI number: 221635980

Start: 567736

End: 570006

Strand: Reverse

Name: pleD [H]

Synonym: trd_A0574

Alternate gene names: 221635980

Gene position: 570006-567736 (Counterclockwise)

Preceding gene: 221635981

Following gene: 221635976

Centisome position: 62.11

GC content: 62.92

Gene sequence:

>2271_bases
ATGGCATGGGACGATCTCGACCTCCTCATCATCGATCAGGATCCAGTTCGGCGAGGAGCGATCGCGAGGATCGCCCGGGA
ACTGGGTGTGCGGCGTGTCCAAGAGCGAGAGGAGTGGGTTCCTCCGGCTGAGGGTATTCGTGTACCGGGTGTCCGTGTGG
TGCTTCTCGATGCGGATATCTCGACCCCTCATACATCGGAACTCACTGAAGCGGCCAGTTCCGGCATGCTCCTCGTCGCG
CTGGTGCGGAGCGAGCGCGGAGCGGAACCGGAACTGCTGGCTGCTGGCTTCGGTGCGGTGCTGTACGACCCGGTAACCGC
GGTCGACTTGGAGCGCATCTTCGCGCTCGCACGCACACTCGCGTCGCAGGGACAGGAGCGAGAACGCCGCCAGTTCCTGC
GATTCAACGCGCTCCGACAGGTCGAAACCGACCTCGCCTTGTTGGCGGAGCTGAGTCCTGATTGGATGCAACGGAGTCTC
CTGCTTTTGCAGCGCATCCTCGATGTCGCAGCGCTCGGGCTGTGGCGGATCGATTGGGAGCAAGACCGGCTGGTTTGCGA
AGGAGCCGTCGGTCTACCGGAGGAGTTCATTCGCCAAGTGGAAGAGCGTGCTCAGGGCCGGGCAGCCGAACTGGTTCAGC
GGGTGCTGGAATCGGTGGTGCAACCGGTGGACATGCGCCCTGATTCGACTGACGAGCGAGTGATCACCGACGCCGCGGTG
CGCCGAGCATGTGGACTGGAAGCGGGTATCGTGGTGCCAGTGCGTCGGACTGGTCGCGTCGCTGCGTTGCTTTCGATCTA
CTTCCGGCGATATGAGGAAGTGAATCGCGAGGAGATTGGCTTGTTCGATGCGGCGGCGGAGGCGCTGGCGACTGCCTGGG
CAATCAGCGAGGTCAGGCGCGAACTTTTCTTGAACCAGCAGCAGTACCGCGCGTTGGTGGAAGAACAACCGGTCGGGGTG
CTGCTTTGCGAGCTGAACGGAACCGTGCGATTGGCGAATGGGGCAGCCGCTCGCCTTTTGGGATACGAGCGTCCGGAACG
GCTAGCCGGTACTCGGCTACCCGAGGTCGTGCGCTCCCTCGCGCCGATACCGTGGGAGGAGTGGTGCCAGCGCCCAGTCG
GTGCTCCGGCAGCGGAAGCGACTATCCCTGTGCTGACGCTGGACAAGCACCTCCGCATCATCGAGTTCCACGCACGTGTC
ATCGAGTTACCGATGAGCACGCGCGATTGGTTCCCACAAGTTCAACTGGTACTCCGAGACGTGACGCTCGAGCGACGCCG
TCTCATGGAGTTGGAGCTCCTGCACGACTTGACTCGTATGGTATCGGAGGATCGCAATTTGGATTCGGCCTTCGAAATGG
TGGCGGACCGTCTCCAGCGCGAGTTCGGCTATGCACTCGTCGGCTTGGCGCTGCTGAGTGCTGACGGTACACGATTCGTC
GGCCGGGCGGTCCGCGTGGAGGGTGGGCTGACGTTCAACGAGTGGCGGGCCGATCGCGGAGTGACCGGACGCGCAGTGCG
TGAGAATCGTGCTCAGCTTGTTGTCGATGTTCGGCAAGATCCAGATTACTTCGATCCGCAACCAGATCTGCCGATGGAGA
GTGAAGTCGTGGCTGTCCTGCGGCGGGAGGGTAAGCCGATCGGAGTTCTCAACATCGAGAGCCGGCGCGGTCACCGGTTG
GATCAAGAGGACTTGCGCCTCGCCTTGAACGTGGCAGTGCACCTGGAGCTGTTGATGCGCCAGGTGGAACTGACCGAGCA
ACTCGAACGGAAGGCACTGACCGACCCGCTGACGGGACAGCCGAACCGCCGAGCCCTGCTCGATCACTTGCGCCGTGCCC
TGGGCGATCGGCGCGTCGAGCACGTCGCAGTGATCCTGATCGATTTCGACGGTTTCAAGGTTCTCAACGATACCAAAGGC
CACTTGTTCGGTGACGCACTTCTGCAAGCGGTCGCAGAACGACTGGCGCGCTCGCTCCGACCGAGTGACCTCTTGGCCCG
CTACGGCGGTGACGAGTTCGCGGTGGTCCTCGCTGGCGTGGATCTCGCAGTGGCGCACGACATCGCTGAACGCTTGCGGC
GAGCGGTCGCCGACACATCGTTCCAGGTGCGCGACGAGGTCGTTTCTTTGACGATCTCGCTCGGGGTGGCAGCCTCGCCG
CGGCATGGTGACACGCCAGAAACATTGTTGCGAGCAGCTGACGAAGCGCTGTATGCCGCCAAGCGGCGGGGTGGCAATTC
GATCGCCATCGCCGGAGAATGGTCGCCCTGA

Upstream 100 bases:

>100_bases
CTGACGGGATAGGAAGAAAGTCCTACAGCGGCTCGACGCACGGTCGGATACGATCACGGTACGTCTTGGGAGGGAAGGGT
TTCGTGGGACTGCTCCTGTG

Downstream 100 bases:

>100_bases
ATCAGCCTTCAAGGGAGTGCAAGGAGTTCGTGCGGGGCGCGGGTGAGCCGCTCGGCGCCTTCTGCGGTGATGAGCACGAC
ATCCTCGATCCGGAGCCCCC

Product: ggdef domain protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 756; Mature: 755

Protein sequence:

>756_residues
MAWDDLDLLIIDQDPVRRGAIARIARELGVRRVQEREEWVPPAEGIRVPGVRVVLLDADISTPHTSELTEAASSGMLLVA
LVRSERGAEPELLAAGFGAVLYDPVTAVDLERIFALARTLASQGQERERRQFLRFNALRQVETDLALLAELSPDWMQRSL
LLLQRILDVAALGLWRIDWEQDRLVCEGAVGLPEEFIRQVEERAQGRAAELVQRVLESVVQPVDMRPDSTDERVITDAAV
RRACGLEAGIVVPVRRTGRVAALLSIYFRRYEEVNREEIGLFDAAAEALATAWAISEVRRELFLNQQQYRALVEEQPVGV
LLCELNGTVRLANGAAARLLGYERPERLAGTRLPEVVRSLAPIPWEEWCQRPVGAPAAEATIPVLTLDKHLRIIEFHARV
IELPMSTRDWFPQVQLVLRDVTLERRRLMELELLHDLTRMVSEDRNLDSAFEMVADRLQREFGYALVGLALLSADGTRFV
GRAVRVEGGLTFNEWRADRGVTGRAVRENRAQLVVDVRQDPDYFDPQPDLPMESEVVAVLRREGKPIGVLNIESRRGHRL
DQEDLRLALNVAVHLELLMRQVELTEQLERKALTDPLTGQPNRRALLDHLRRALGDRRVEHVAVILIDFDGFKVLNDTKG
HLFGDALLQAVAERLARSLRPSDLLARYGGDEFAVVLAGVDLAVAHDIAERLRRAVADTSFQVRDEVVSLTISLGVAASP
RHGDTPETLLRAADEALYAAKRRGGNSIAIAGEWSP

Sequences:

>Translated_756_residues
MAWDDLDLLIIDQDPVRRGAIARIARELGVRRVQEREEWVPPAEGIRVPGVRVVLLDADISTPHTSELTEAASSGMLLVA
LVRSERGAEPELLAAGFGAVLYDPVTAVDLERIFALARTLASQGQERERRQFLRFNALRQVETDLALLAELSPDWMQRSL
LLLQRILDVAALGLWRIDWEQDRLVCEGAVGLPEEFIRQVEERAQGRAAELVQRVLESVVQPVDMRPDSTDERVITDAAV
RRACGLEAGIVVPVRRTGRVAALLSIYFRRYEEVNREEIGLFDAAAEALATAWAISEVRRELFLNQQQYRALVEEQPVGV
LLCELNGTVRLANGAAARLLGYERPERLAGTRLPEVVRSLAPIPWEEWCQRPVGAPAAEATIPVLTLDKHLRIIEFHARV
IELPMSTRDWFPQVQLVLRDVTLERRRLMELELLHDLTRMVSEDRNLDSAFEMVADRLQREFGYALVGLALLSADGTRFV
GRAVRVEGGLTFNEWRADRGVTGRAVRENRAQLVVDVRQDPDYFDPQPDLPMESEVVAVLRREGKPIGVLNIESRRGHRL
DQEDLRLALNVAVHLELLMRQVELTEQLERKALTDPLTGQPNRRALLDHLRRALGDRRVEHVAVILIDFDGFKVLNDTKG
HLFGDALLQAVAERLARSLRPSDLLARYGGDEFAVVLAGVDLAVAHDIAERLRRAVADTSFQVRDEVVSLTISLGVAASP
RHGDTPETLLRAADEALYAAKRRGGNSIAIAGEWSP
>Mature_755_residues
AWDDLDLLIIDQDPVRRGAIARIARELGVRRVQEREEWVPPAEGIRVPGVRVVLLDADISTPHTSELTEAASSGMLLVAL
VRSERGAEPELLAAGFGAVLYDPVTAVDLERIFALARTLASQGQERERRQFLRFNALRQVETDLALLAELSPDWMQRSLL
LLQRILDVAALGLWRIDWEQDRLVCEGAVGLPEEFIRQVEERAQGRAAELVQRVLESVVQPVDMRPDSTDERVITDAAVR
RACGLEAGIVVPVRRTGRVAALLSIYFRRYEEVNREEIGLFDAAAEALATAWAISEVRRELFLNQQQYRALVEEQPVGVL
LCELNGTVRLANGAAARLLGYERPERLAGTRLPEVVRSLAPIPWEEWCQRPVGAPAAEATIPVLTLDKHLRIIEFHARVI
ELPMSTRDWFPQVQLVLRDVTLERRRLMELELLHDLTRMVSEDRNLDSAFEMVADRLQREFGYALVGLALLSADGTRFVG
RAVRVEGGLTFNEWRADRGVTGRAVRENRAQLVVDVRQDPDYFDPQPDLPMESEVVAVLRREGKPIGVLNIESRRGHRLD
QEDLRLALNVAVHLELLMRQVELTEQLERKALTDPLTGQPNRRALLDHLRRALGDRRVEHVAVILIDFDGFKVLNDTKGH
LFGDALLQAVAERLARSLRPSDLLARYGGDEFAVVLAGVDLAVAHDIAERLRRAVADTSFQVRDEVVSLTISLGVAASPR
HGDTPETLLRAADEALYAAKRRGGNSIAIAGEWSP

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=210, Percent_Identity=36.6666666666667, Blast_Score=106, Evalue=7e-24,
Organism=Escherichia coli, GI1787262, Length=161, Percent_Identity=35.4037267080745, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1787541, Length=169, Percent_Identity=37.2781065088757, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI87082007, Length=169, Percent_Identity=36.094674556213, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1788381, Length=164, Percent_Identity=35.9756097560976, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1788085, Length=177, Percent_Identity=34.4632768361582, Blast_Score=87, Evalue=5e-18,
Organism=Escherichia coli, GI87081881, Length=180, Percent_Identity=35.5555555555556, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1786584, Length=181, Percent_Identity=33.1491712707182, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787802, Length=168, Percent_Identity=32.1428571428571, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI87081921, Length=215, Percent_Identity=30.2325581395349, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI87081977, Length=155, Percent_Identity=32.258064516129, Blast_Score=77, Evalue=5e-15,
Organism=Escherichia coli, GI1787816, Length=160, Percent_Identity=30.625, Blast_Score=76, Evalue=8e-15,
Organism=Escherichia coli, GI87081974, Length=180, Percent_Identity=28.8888888888889, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788956, Length=160, Percent_Identity=34.375, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1787056, Length=153, Percent_Identity=35.9477124183007, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 84650; Mature: 84519

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50112 PAS ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAWDDLDLLIIDQDPVRRGAIARIARELGVRRVQEREEWVPPAEGIRVPGVRVVLLDADI
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCEEEEEEECCC
STPHTSELTEAASSGMLLVALVRSERGAEPELLAAGFGAVLYDPVTAVDLERIFALARTL
CCCCHHHHHHHHCCCEEEEEEEHHCCCCCCHHHHHCCCHHHHCCHHHHHHHHHHHHHHHH
ASQGQERERRQFLRFNALRQVETDLALLAELSPDWMQRSLLLLQRILDVAALGLWRIDWE
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEECCC
QDRLVCEGAVGLPEEFIRQVEERAQGRAAELVQRVLESVVQPVDMRPDSTDERVITDAAV
CCCEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
RRACGLEAGIVVPVRRTGRVAALLSIYFRRYEEVNREEIGLFDAAAEALATAWAISEVRR
HHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHH
ELFLNQQQYRALVEEQPVGVLLCELNGTVRLANGAAARLLGYERPERLAGTRLPEVVRSL
HHHCCHHHHHHHHHCCCCEEEEEECCCEEEECCCHHHHHHCCCCCHHHHCCCHHHHHHHH
APIPWEEWCQRPVGAPAAEATIPVLTLDKHLRIIEFHARVIELPMSTRDWFPQVQLVLRD
CCCCHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
VTLERRRLMELELLHDLTRMVSEDRNLDSAFEMVADRLQREFGYALVGLALLSADGTRFV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
GRAVRVEGGLTFNEWRADRGVTGRAVRENRAQLVVDVRQDPDYFDPQPDLPMESEVVAVL
CEEEEEECCCCHHHHHCCCCCCCHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHH
RREGKPIGVLNIESRRGHRLDQEDLRLALNVAVHLELLMRQVELTEQLERKALTDPLTGQ
HHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PNRRALLDHLRRALGDRRVEHVAVILIDFDGFKVLNDTKGHLFGDALLQAVAERLARSLR
CCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHCC
PSDLLARYGGDEFAVVLAGVDLAVAHDIAERLRRAVADTSFQVRDEVVSLTISLGVAASP
HHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCC
RHGDTPETLLRAADEALYAAKRRGGNSIAIAGEWSP
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
>Mature Secondary Structure 
AWDDLDLLIIDQDPVRRGAIARIARELGVRRVQEREEWVPPAEGIRVPGVRVVLLDADI
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCEEEEEEECCC
STPHTSELTEAASSGMLLVALVRSERGAEPELLAAGFGAVLYDPVTAVDLERIFALARTL
CCCCHHHHHHHHCCCEEEEEEEHHCCCCCCHHHHHCCCHHHHCCHHHHHHHHHHHHHHHH
ASQGQERERRQFLRFNALRQVETDLALLAELSPDWMQRSLLLLQRILDVAALGLWRIDWE
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEECCC
QDRLVCEGAVGLPEEFIRQVEERAQGRAAELVQRVLESVVQPVDMRPDSTDERVITDAAV
CCCEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
RRACGLEAGIVVPVRRTGRVAALLSIYFRRYEEVNREEIGLFDAAAEALATAWAISEVRR
HHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHH
ELFLNQQQYRALVEEQPVGVLLCELNGTVRLANGAAARLLGYERPERLAGTRLPEVVRSL
HHHCCHHHHHHHHHCCCCEEEEEECCCEEEECCCHHHHHHCCCCCHHHHCCCHHHHHHHH
APIPWEEWCQRPVGAPAAEATIPVLTLDKHLRIIEFHARVIELPMSTRDWFPQVQLVLRD
CCCCHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
VTLERRRLMELELLHDLTRMVSEDRNLDSAFEMVADRLQREFGYALVGLALLSADGTRFV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
GRAVRVEGGLTFNEWRADRGVTGRAVRENRAQLVVDVRQDPDYFDPQPDLPMESEVVAVL
CEEEEEECCCCHHHHHCCCCCCCHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHH
RREGKPIGVLNIESRRGHRLDQEDLRLALNVAVHLELLMRQVELTEQLERKALTDPLTGQ
HHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PNRRALLDHLRRALGDRRVEHVAVILIDFDGFKVLNDTKGHLFGDALLQAVAERLARSLR
CCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHCC
PSDLLARYGGDEFAVVLAGVDLAVAHDIAERLRRAVADTSFQVRDEVVSLTISLGVAASP
HHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCC
RHGDTPETLLRAADEALYAAKRRGGNSIAIAGEWSP
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA