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The map label for this gene is yfiS [H]

Identifier: 221635981

GI number: 221635981

Start: 570116

End: 571399

Strand: Reverse

Name: yfiS [H]

Synonym: trd_A0575

Alternate gene names: 221635981

Gene position: 571399-570116 (Counterclockwise)

Preceding gene: 221635983

Following gene: 221635980

Centisome position: 62.26

GC content: 65.26

Gene sequence:

>1284_bases
GTGCGAGTTCGCTGGGGGGAAGCGCTGGTTGCGCTCCGGTTCCGGGAGTTTCGCTTGCTCTGGGCCGGGCAGTTCGTCTC
CATGCTCGGCACGCAGATGCAGACCGTCGCCCTGAGCTGGTTGGTCTATGACTTGACAGGATCGCCGGCACAGCTCGGCG
GTGTGGCTCTGGTGCGAGCGATCCCGACAATGGTGCTCTCGCTCTTCGGCGGCACGTTGGCCGATCAGATGGATCGGCGT
CGACTTCTTTTACTCACGCAATCGACGGCCGCGTTTCTCTTGGGAGTGCTCGCGCTCGTGGTCTCCTTGGGGATGACGTC
GCTCCCGCTCTTGTATCTCCTCGCGTTCACCTCGGCAGCAGCCATGTCGTTCGATGCACCGGCTCGTCAGGCCCTCATTC
CTTTGCTGGTACCTCGCGAACGACTCGCCAACGCGCTGGCTTTGAATGTCCTGGCCTGGGATGTGGCGGCCATCGCTGGG
CCGGCCCTAGGTGGGCTGGTGATCGCGCATGTGAGTGTGGCCGCCGCATTTTGGGTCGATGCCGCGAGCTATTTGGCTGT
GGTGGCAGCCTTGCTGGCCATGCGGCGCGATCTGCCCGTGCCGAAACTCGAGCGGCGGGGGTGGACTGCTTTCATGGACG
GGTTGCGGTTCGTCCGCCACCGTCCGCTCCTCTGGCAACTGATGCTCCTCGACTTCTTTGCAGTCCTCCTGGCCTCCTCG
ACGGGACTTCTCCCCGTGTTCGCTCGCGACGTTCTCAGGGTCGGGCCAGATGGACTCGGGCTCTTGTTCGCAGCCCCCAG
TGCTGGAGCGATCGTCGGGGCAGGACTGTTCGCGCTGCTGCCGACACCGCGTTGGCCGGGCCAGGTGGTTGCCTGGGTCG
TCGTCGGGTATGGTGCGGCGCTGGCCGTCTTCGGCATAAGTCGCCTGTTCGTCTTGTCATTCCTCCTGTTGGCACTAGCG
GGAGCCTTGGACGCGGTCGCTATGGCGCTTCGCCATACCGTCCGGCAGTTGGCGACCCCTGATGCCTATCGCGGCCGGGT
GGGAGCGATTGCAGCGGTCTTTGCGGCAGGTGGCCCGCGACTCGGACAGTTCCAAGCCGGTGTGCTCGCAGGTGCTGTGG
GGCCAGCCGGTGCGATGGTGATCGGCGGGGTGGGCTGTGTCCTGGTAGCGGTGGCGTCTCGCTGGTGGGCTCCGGAACTC
TGGCGATTTTGCGGAGAAGAGCTGTTCGAAGCGGCTGGTGCCGGGCACCGGAAACCTCGAGCGAAACCCTCGCCCTCGGA
GTAG

Upstream 100 bases:

>100_bases
GAGCGTGCATGCGGACGATCCTTTCGTTCGCGCGCAGTGTACCATCGGTCGAGGCTTGCCGGCAGAAGTGTCTCGCAGAT
GTCGTTTCGGGGGCAGGCAG

Downstream 100 bases:

>100_bases
CACAGCGTGCTGACGGGATAGGAAGAAAGTCCTACAGCGGCTCGACGCACGGTCGGATACGATCACGGTACGTCTTGGGA
GGGAAGGGTTTCGTGGGACT

Product: major facilitator superfamily MFS_1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MRVRWGEALVALRFREFRLLWAGQFVSMLGTQMQTVALSWLVYDLTGSPAQLGGVALVRAIPTMVLSLFGGTLADQMDRR
RLLLLTQSTAAFLLGVLALVVSLGMTSLPLLYLLAFTSAAAMSFDAPARQALIPLLVPRERLANALALNVLAWDVAAIAG
PALGGLVIAHVSVAAAFWVDAASYLAVVAALLAMRRDLPVPKLERRGWTAFMDGLRFVRHRPLLWQLMLLDFFAVLLASS
TGLLPVFARDVLRVGPDGLGLLFAAPSAGAIVGAGLFALLPTPRWPGQVVAWVVVGYGAALAVFGISRLFVLSFLLLALA
GALDAVAMALRHTVRQLATPDAYRGRVGAIAAVFAAGGPRLGQFQAGVLAGAVGPAGAMVIGGVGCVLVAVASRWWAPEL
WRFCGEELFEAAGAGHRKPRAKPSPSE

Sequences:

>Translated_427_residues
MRVRWGEALVALRFREFRLLWAGQFVSMLGTQMQTVALSWLVYDLTGSPAQLGGVALVRAIPTMVLSLFGGTLADQMDRR
RLLLLTQSTAAFLLGVLALVVSLGMTSLPLLYLLAFTSAAAMSFDAPARQALIPLLVPRERLANALALNVLAWDVAAIAG
PALGGLVIAHVSVAAAFWVDAASYLAVVAALLAMRRDLPVPKLERRGWTAFMDGLRFVRHRPLLWQLMLLDFFAVLLASS
TGLLPVFARDVLRVGPDGLGLLFAAPSAGAIVGAGLFALLPTPRWPGQVVAWVVVGYGAALAVFGISRLFVLSFLLLALA
GALDAVAMALRHTVRQLATPDAYRGRVGAIAAVFAAGGPRLGQFQAGVLAGAVGPAGAMVIGGVGCVLVAVASRWWAPEL
WRFCGEELFEAAGAGHRKPRAKPSPSE
>Mature_427_residues
MRVRWGEALVALRFREFRLLWAGQFVSMLGTQMQTVALSWLVYDLTGSPAQLGGVALVRAIPTMVLSLFGGTLADQMDRR
RLLLLTQSTAAFLLGVLALVVSLGMTSLPLLYLLAFTSAAAMSFDAPARQALIPLLVPRERLANALALNVLAWDVAAIAG
PALGGLVIAHVSVAAAFWVDAASYLAVVAALLAMRRDLPVPKLERRGWTAFMDGLRFVRHRPLLWQLMLLDFFAVLLASS
TGLLPVFARDVLRVGPDGLGLLFAAPSAGAIVGAGLFALLPTPRWPGQVVAWVVVGYGAALAVFGISRLFVLSFLLLALA
GALDAVAMALRHTVRQLATPDAYRGRVGAIAAVFAAGGPRLGQFQAGVLAGAVGPAGAMVIGGVGCVLVAVASRWWAPEL
WRFCGEELFEAAGAGHRKPRAKPSPSE

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1786806, Length=334, Percent_Identity=28.4431137724551, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45100; Mature: 45100

Theoretical pI: Translated: 11.47; Mature: 11.47

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVRWGEALVALRFREFRLLWAGQFVSMLGTQMQTVALSWLVYDLTGSPAQLGGVALVRA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHH
IPTMVLSLFGGTLADQMDRRRLLLLTQSTAAFLLGVLALVVSLGMTSLPLLYLLAFTSAA
HHHHHHHHHCCHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
AMSFDAPARQALIPLLVPRERLANALALNVLAWDVAAIAGPALGGLVIAHVSVAAAFWVD
HHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
AASYLAVVAALLAMRRDLPVPKLERRGWTAFMDGLRFVRHRPLLWQLMLLDFFAVLLASS
HHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
TGLLPVFARDVLRVGPDGLGLLFAAPSAGAIVGAGLFALLPTPRWPGQVVAWVVVGYGAA
CCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LAVFGISRLFVLSFLLLALAGALDAVAMALRHTVRQLATPDAYRGRVGAIAAVFAAGGPR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHCCCCC
LGQFQAGVLAGAVGPAGAMVIGGVGCVLVAVASRWWAPELWRFCGEELFEAAGAGHRKPR
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCC
AKPSPSE
CCCCCCC
>Mature Secondary Structure
MRVRWGEALVALRFREFRLLWAGQFVSMLGTQMQTVALSWLVYDLTGSPAQLGGVALVRA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHH
IPTMVLSLFGGTLADQMDRRRLLLLTQSTAAFLLGVLALVVSLGMTSLPLLYLLAFTSAA
HHHHHHHHHCCHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
AMSFDAPARQALIPLLVPRERLANALALNVLAWDVAAIAGPALGGLVIAHVSVAAAFWVD
HHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
AASYLAVVAALLAMRRDLPVPKLERRGWTAFMDGLRFVRHRPLLWQLMLLDFFAVLLASS
HHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
TGLLPVFARDVLRVGPDGLGLLFAAPSAGAIVGAGLFALLPTPRWPGQVVAWVVVGYGAA
CCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LAVFGISRLFVLSFLLLALAGALDAVAMALRHTVRQLATPDAYRGRVGAIAAVFAAGGPR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHCCCCC
LGQFQAGVLAGAVGPAGAMVIGGVGCVLVAVASRWWAPELWRFCGEELFEAAGAGHRKPR
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCC
AKPSPSE
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]