| Definition | Thermomicrobium roseum DSM 5159 plasmid unnamed, complete sequence. |
|---|---|
| Accession | NC_011961 |
| Length | 917,738 |
Click here to switch to the map view.
The map label for this gene is xsc [H]
Identifier: 221635734
GI number: 221635734
Start: 314715
End: 316361
Strand: Reverse
Name: xsc [H]
Synonym: trd_A0328
Alternate gene names: 221635734
Gene position: 316361-314715 (Counterclockwise)
Preceding gene: 221635735
Following gene: 221635733
Centisome position: 34.47
GC content: 59.56
Gene sequence:
>1647_bases GTGGCTAAGGTCTACGAGCTCCTTTCGCGCCAGCTCATCGGCCTCGGTTCCGATACGATCTTTGGCCTCGTAGGGAGTGG CAACTTCTTCCTGGCCGAGAGTCTCGTCACCCATGGTGCGAAGTTCGTGGCTGCGCGACATGAGGCCGGAGCCTTAGCGA TGGCCGATGCCTGGGCTCGTCTGACACAAAAGGTCGGAATCTGCACTGTTCACATGGGTCCAGGTTTCACGAACATCATT ACACCACTCGTCGAAGCTGCAAAAGCACGGACACCGCTCCTCGTCCTGACCGCCGATGTGTCGACAGGGGCCGTTACGTC CACGTTCGCCATCGATATCGATGCCTTGGCCACCGCCAGTGGAGCCATCCCTGAACGGTTGTGGAAGAACTCCACAGCAA CCCAAGACCTTGTCCGTGCCTATCGCCGCGCTCGATACGAGCGTCGGCCAGTCGTCTTCGTCATGCCACAAGACATCCAA GCAGCGGAGTCCACGGCAGCTGTCATAGCTCCAGAGGACAATGCCTACTTTTCTGTGATCCCTGACCGAAGCATACTCGA GAAGATCGTCTCCCTCGTTCGGGACTCGCGTCACCCTCTCATTTTGGCAGGCCGCGGCTCAGTTCTCGCGGGAGCGAGGG CAGGTCTCGAGAAACTCGCCGAAGCCATTGGAGCTCTTCTCGCCACGACAGCGAATGCCCACGGACTGTTCGCTGGGAAC CCCTGGTCGATCGGTATCGCTGGCGGATTCTCGTCTCCCGGAGCAGTCGAACTCTTCCGACAGGCCGATCTGATTTTGGC CTTCGGCGCCTCCCTCACCGTTTGGACCCGCCGCCACGGTCGTCTCTTTCCAGAGTCGTGCCCGATCGTGCAGATCGACC TCGACCCCGCCGCGCTGCACCGCCAATACCCGGTCACGCTCGCTTGCGTCGGCGACGCCCGTGCCACGGCAGAAGCACTA CTCGAGCTCTTGCCCGACAAGCCAGTAGGGCAATGGCGAACACCGTCGACGCTCCAGTTCATTCAGGAACACTCCTGGAT TAACACACCGTTCGAGGATGCCAGCACGGAGAACACGATCGACCCGAGAACGCTTTCACTGTTCCTGGAACAGAACCTTC CACCGGATCGTATCCTCGTCCTCGACTCTGGTCACTTCATGGCATACCCGGTGATGTATCTGAGTGTCCCAGATGAAAAA GGTTTTGTTTTCACGCAAGCATTACAGTCGATCGGCTTCGGTTTGCCGAATGCGATCGGAGCAGCCATCGCCTATCCAGA TCGTCTCACCATCGCAGCGACTGGTGACGGAGGACTGCTGCTCTCGTTGAGCGAACTCGAGACAGTCGCACGCTATCGGC TCCCCATCCTCATCGTGGTTTACAACGATGCCGCCTATGGGGCCGAGGTTCATCACTTCGCTCCGATGGGTTGGCCAACT TCCACCGTGCAATTCCCTGACACGAACATCGCCGAGCTCGCTCGCGCCCTGGGTATGCACGCCTTTACCGTCCGCACACA GCGCGACCTGGAATCGCTCCGGGAGGCGCTCTCCGATCGCTCCTGCTATCCCATGCTGCTGGATGCCAAGGTCAACCCAG CGGTACGCGGCGGAGAATGGTTCGATCTGGCTTTCGAGGGTCACTAA
Upstream 100 bases:
>100_bases AAATCTTCGCCGAGCAGGTTCGACTCCAACAGGGTCTGACGACGCCAGCCCAAGCTGTCACGATATAGGTTTTGGTCAGT TCAAGAGAAAGGGGTTCACC
Downstream 100 bases:
>100_bases CTCGGTGTTCTGTTTGGAAAGGAGGACACATGGCCCTGTGGTTGACCCATGTCGGCGTTCGCGTGCCGAATCTGCAGGAC TCCATCGAGTTTCACACGTC
Product: thiamine pyrophosphate enzyme, central domain family
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 548; Mature: 547
Protein sequence:
>548_residues MAKVYELLSRQLIGLGSDTIFGLVGSGNFFLAESLVTHGAKFVAARHEAGALAMADAWARLTQKVGICTVHMGPGFTNII TPLVEAAKARTPLLVLTADVSTGAVTSTFAIDIDALATASGAIPERLWKNSTATQDLVRAYRRARYERRPVVFVMPQDIQ AAESTAAVIAPEDNAYFSVIPDRSILEKIVSLVRDSRHPLILAGRGSVLAGARAGLEKLAEAIGALLATTANAHGLFAGN PWSIGIAGGFSSPGAVELFRQADLILAFGASLTVWTRRHGRLFPESCPIVQIDLDPAALHRQYPVTLACVGDARATAEAL LELLPDKPVGQWRTPSTLQFIQEHSWINTPFEDASTENTIDPRTLSLFLEQNLPPDRILVLDSGHFMAYPVMYLSVPDEK GFVFTQALQSIGFGLPNAIGAAIAYPDRLTIAATGDGGLLLSLSELETVARYRLPILIVVYNDAAYGAEVHHFAPMGWPT STVQFPDTNIAELARALGMHAFTVRTQRDLESLREALSDRSCYPMLLDAKVNPAVRGGEWFDLAFEGH
Sequences:
>Translated_548_residues MAKVYELLSRQLIGLGSDTIFGLVGSGNFFLAESLVTHGAKFVAARHEAGALAMADAWARLTQKVGICTVHMGPGFTNII TPLVEAAKARTPLLVLTADVSTGAVTSTFAIDIDALATASGAIPERLWKNSTATQDLVRAYRRARYERRPVVFVMPQDIQ AAESTAAVIAPEDNAYFSVIPDRSILEKIVSLVRDSRHPLILAGRGSVLAGARAGLEKLAEAIGALLATTANAHGLFAGN PWSIGIAGGFSSPGAVELFRQADLILAFGASLTVWTRRHGRLFPESCPIVQIDLDPAALHRQYPVTLACVGDARATAEAL LELLPDKPVGQWRTPSTLQFIQEHSWINTPFEDASTENTIDPRTLSLFLEQNLPPDRILVLDSGHFMAYPVMYLSVPDEK GFVFTQALQSIGFGLPNAIGAAIAYPDRLTIAATGDGGLLLSLSELETVARYRLPILIVVYNDAAYGAEVHHFAPMGWPT STVQFPDTNIAELARALGMHAFTVRTQRDLESLREALSDRSCYPMLLDAKVNPAVRGGEWFDLAFEGH >Mature_547_residues AKVYELLSRQLIGLGSDTIFGLVGSGNFFLAESLVTHGAKFVAARHEAGALAMADAWARLTQKVGICTVHMGPGFTNIIT PLVEAAKARTPLLVLTADVSTGAVTSTFAIDIDALATASGAIPERLWKNSTATQDLVRAYRRARYERRPVVFVMPQDIQA AESTAAVIAPEDNAYFSVIPDRSILEKIVSLVRDSRHPLILAGRGSVLAGARAGLEKLAEAIGALLATTANAHGLFAGNP WSIGIAGGFSSPGAVELFRQADLILAFGASLTVWTRRHGRLFPESCPIVQIDLDPAALHRQYPVTLACVGDARATAEALL ELLPDKPVGQWRTPSTLQFIQEHSWINTPFEDASTENTIDPRTLSLFLEQNLPPDRILVLDSGHFMAYPVMYLSVPDEKG FVFTQALQSIGFGLPNAIGAAIAYPDRLTIAATGDGGLLLSLSELETVARYRLPILIVVYNDAAYGAEVHHFAPMGWPTS TVQFPDTNIAELARALGMHAFTVRTQRDLESLREALSDRSCYPMLLDAKVNPAVRGGEWFDLAFEGH
Specific function: Valine and isoleucine biosynthesis; first step. [C]
COG id: COG0028
COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the TPP enzyme family [H]
Homologues:
Organism=Homo sapiens, GI93004078, Length=565, Percent_Identity=25.3097345132743, Blast_Score=113, Evalue=5e-25, Organism=Escherichia coli, GI87081685, Length=570, Percent_Identity=26.140350877193, Blast_Score=165, Evalue=7e-42, Organism=Escherichia coli, GI1787096, Length=550, Percent_Identity=25.2727272727273, Blast_Score=142, Evalue=5e-35, Organism=Escherichia coli, GI1790104, Length=512, Percent_Identity=23.828125, Blast_Score=114, Evalue=2e-26, Organism=Escherichia coli, GI1786717, Length=468, Percent_Identity=24.5726495726496, Blast_Score=100, Evalue=2e-22, Organism=Escherichia coli, GI1788716, Length=541, Percent_Identity=24.3992606284658, Blast_Score=98, Evalue=1e-21, Organism=Caenorhabditis elegans, GI17542570, Length=569, Percent_Identity=24.6045694200351, Blast_Score=108, Evalue=6e-24, Organism=Saccharomyces cerevisiae, GI6323755, Length=579, Percent_Identity=24.006908462867, Blast_Score=125, Evalue=2e-29, Organism=Drosophila melanogaster, GI19922626, Length=574, Percent_Identity=21.7770034843206, Blast_Score=100, Evalue=2e-21,
Paralogues:
None
Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR017820 - InterPro: IPR012000 - InterPro: IPR012001 - InterPro: IPR000399 - InterPro: IPR011766 [H]
Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]
EC number: =2.3.3.15 [H]
Molecular weight: Translated: 59107; Mature: 58976
Theoretical pI: Translated: 5.76; Mature: 5.76
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 1.6 %Met (Translated Protein) 2.4 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 1.5 %Met (Mature Protein) 2.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MAKVYELLSRQLIGLGSDTIFGLVGSGNFFLAESLVTHGAKFVAARHEAGALAMADAWAR CCHHHHHHHHHHHCCCCCEEEEEEECCCEEEHHHHHHCCHHEEEECCCCCCCHHHHHHHH LTQKVGICTVHMGPGFTNIITPLVEAAKARTPLLVLTADVSTGAVTSTFAIDIDALATAS HHHHCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEEEHHHHHCCC GAIPERLWKNSTATQDLVRAYRRARYERRPVVFVMPQDIQAAESTAAVIAPEDNAYFSVI CCCHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEECCCCCEEEEC PDRSILEKIVSLVRDSRHPLILAGRGSVLAGARAGLEKLAEAIGALLATTANAHGLFAGN CCHHHHHHHHHHHHCCCCCEEEECCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEEECCC PWSIGIAGGFSSPGAVELFRQADLILAFGASLTVWTRRHGRLFPESCPIVQIDLDPAALH CEEEEEECCCCCCCHHHHHHHCCEEEEECCEEEEEECCCCCCCCCCCCEEEEECCHHHHC RQYPVTLACVGDARATAEALLELLPDKPVGQWRTPSTLQFIQEHSWINTPFEDASTENTI CCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC DPRTLSLFLEQNLPPDRILVLDSGHFMAYPVMYLSVPDEKGFVFTQALQSIGFGLPNAIG CCHHEEEEECCCCCCCEEEEEECCCEEEEEEEEEECCCCCCCHHHHHHHHHCCCCCHHHH AAIAYPDRLTIAATGDGGLLLSLSELETVARYRLPILIVVYNDAAYGAEVHHFAPMGWPT HHEECCCEEEEEEECCCCEEEEHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCCC STVQFPDTNIAELARALGMHAFTVRTQRDLESLREALSDRSCYPMLLDAKVNPAVRGGEW CEEECCCCCHHHHHHHHCCEEEEEECHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCE FDLAFEGH EEEEEECC >Mature Secondary Structure AKVYELLSRQLIGLGSDTIFGLVGSGNFFLAESLVTHGAKFVAARHEAGALAMADAWAR CHHHHHHHHHHHCCCCCEEEEEEECCCEEEHHHHHHCCHHEEEECCCCCCCHHHHHHHH LTQKVGICTVHMGPGFTNIITPLVEAAKARTPLLVLTADVSTGAVTSTFAIDIDALATAS HHHHCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEEEHHHHHCCC GAIPERLWKNSTATQDLVRAYRRARYERRPVVFVMPQDIQAAESTAAVIAPEDNAYFSVI CCCHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEECCCCCEEEEC PDRSILEKIVSLVRDSRHPLILAGRGSVLAGARAGLEKLAEAIGALLATTANAHGLFAGN CCHHHHHHHHHHHHCCCCCEEEECCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEEECCC PWSIGIAGGFSSPGAVELFRQADLILAFGASLTVWTRRHGRLFPESCPIVQIDLDPAALH CEEEEEECCCCCCCHHHHHHHCCEEEEECCEEEEEECCCCCCCCCCCCEEEEECCHHHHC RQYPVTLACVGDARATAEALLELLPDKPVGQWRTPSTLQFIQEHSWINTPFEDASTENTI CCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC DPRTLSLFLEQNLPPDRILVLDSGHFMAYPVMYLSVPDEKGFVFTQALQSIGFGLPNAIG CCHHEEEEECCCCCCCEEEEEECCCEEEEEEEEEECCCCCCCHHHHHHHHHCCCCCHHHH AAIAYPDRLTIAATGDGGLLLSLSELETVARYRLPILIVVYNDAAYGAEVHHFAPMGWPT HHEECCCEEEEEEECCCCEEEEHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCCC STVQFPDTNIAELARALGMHAFTVRTQRDLESLREALSDRSCYPMLLDAKVNPAVRGGEW CEEECCCCCHHHHHHHHCCEEEEEECHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCE FDLAFEGH EEEEEECC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11439112; 12358600 [H]