| Definition | Thermomicrobium roseum DSM 5159 plasmid unnamed, complete sequence. |
|---|---|
| Accession | NC_011961 |
| Length | 917,738 |
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The map label for this gene is trpB2 [H]
Identifier: 221635606
GI number: 221635606
Start: 181915
End: 183282
Strand: Reverse
Name: trpB2 [H]
Synonym: trd_A0200
Alternate gene names: 221635606
Gene position: 183282-181915 (Counterclockwise)
Preceding gene: 221635607
Following gene: 221635603
Centisome position: 19.97
GC content: 66.23
Gene sequence:
>1368_bases GTGAGCGCAGCAGAGACCGTCAAGTTCGTGTTGACCGAGGCCGATCTCCCAACGCACTGGTACAACCTGGCCGCCGATCT CCCCACGCTTCCCCCACCGCCCATCCACCCAGGGACCGGGGAACCGGTGGGTCCGGCCGACCTCGAACCCGTCTTTCCGA TGGAACTCATCCTCCAGGAAGTGAGCCGCGAGCGCTGGATCGCGATCCCCGGGCCGGTTCTCGACGTCTACCGACTCTGG CGACCGACGCCACTCTTCCGAGCCCGTCGGCTCGAGCGCGTGCTCGGTACACCAGCGCGCATCTACTACAAGTACGAAGG GGTCAGCCCGGCCGGGAGTCATAAGCCGAACACGGCGGTCGCCCAGGCCTACTACAACAAGCAGGCCGGCGTGAAGCGGC TCACCACCGAGACCGGAGCGGGTCAGTGGGGAAGCTCGCTCGCCATGGCCTGCGCCTTCTTCGGTATGGAACTCAAGGTG TATATGGTGCGGGCCAGCTATCTCCAGAAGCCGTACCGGCGTATCCTGATGGAGACCTGGGGAGCCCAGTGCGTCCCCAG CCCCAGTCCCGACACCGCGTCCGGCCGGCGCTTCTACGAGCAGGATCCCGAGCATCCGGGAAGCCTCGGAATCGCCATTT CCGAGGCGGTCGAGGACGCAGCCTCGCGCGCGGACACCAAGTACTCGCTCGGGAGCGTGCTCAACCACGTGCTCCTGCAC CAGACGGTCATCGGGCTGGAGGCGCTCCGGCAGTTCGAGCTGGCCGGTGACTACCCGGACGTCATCTTTGGCTGTGTCGG GGGCGGTTCCAACTTCGCTGGTCTCACTTTTCCCTTCGTGCGGGACAAGCTTCAGGGGAAGGACGTGCGCATCGTCGCCG TCGAACCGGCCGCCTGCCCGTCGCTCACCCGCGGCGTCTATACCTACGACTTCGGCGATACGGCACGGACGACGCCGCTC CTCAAGATGCATACGCTCGGCCACACCTTCATCCCTGCCCGGATCCACGCCGGTGGCTTGCGCTACCATGGGATGGCACC ACTGGTCAGCCACCTCTACGAGCACGGACTGATCGAGGCGATCGCGCTCCCGCAGACCAAGGTGTTCGAGGGAGCCGTTC TGTTCGCCCGTGCCGAGGGGATCATTCCCGCTCCCGAATCGGCACATGCCGTGCGCGCTGCGATCGACGAGGCCCGCCGC TGCGCCGAGACTGGCGAAGAGAAGGCGATCCTGATCGGGCTGAGCGGGCACGGCCACTTCGATCTGGCAGCCTACGAGGC CTACCTCGCCGGGAAGCTGGAAGACTACGAGTATCCCGAGGAAGCAGTCCGCCAGGCGCTCGCCGAGCTGCCGAAGGTCA GCGGCTGA
Upstream 100 bases:
>100_bases GCCGGGAAGGCGGCTGCAGGACGATCTCTTGCAGGATCCGGTCGTTTCGTTACAATGCCTGCAGTGACGAGCGGCACAAC AGGCAAAGCGGAGGACGAGG
Downstream 100 bases:
>100_bases CCACCGCGACGAGCGACTTGCGACCGCACCCCGCTGCTCCCGTTGCACGATCGGGGCGGCGGGGTGTTTTTCGGGGATAC CTGCTCGCGCTGGCAGCACC
Product: tryptophan synthase subunit beta
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 455; Mature: 454
Protein sequence:
>455_residues MSAAETVKFVLTEADLPTHWYNLAADLPTLPPPPIHPGTGEPVGPADLEPVFPMELILQEVSRERWIAIPGPVLDVYRLW RPTPLFRARRLERVLGTPARIYYKYEGVSPAGSHKPNTAVAQAYYNKQAGVKRLTTETGAGQWGSSLAMACAFFGMELKV YMVRASYLQKPYRRILMETWGAQCVPSPSPDTASGRRFYEQDPEHPGSLGIAISEAVEDAASRADTKYSLGSVLNHVLLH QTVIGLEALRQFELAGDYPDVIFGCVGGGSNFAGLTFPFVRDKLQGKDVRIVAVEPAACPSLTRGVYTYDFGDTARTTPL LKMHTLGHTFIPARIHAGGLRYHGMAPLVSHLYEHGLIEAIALPQTKVFEGAVLFARAEGIIPAPESAHAVRAAIDEARR CAETGEEKAILIGLSGHGHFDLAAYEAYLAGKLEDYEYPEEAVRQALAELPKVSG
Sequences:
>Translated_455_residues MSAAETVKFVLTEADLPTHWYNLAADLPTLPPPPIHPGTGEPVGPADLEPVFPMELILQEVSRERWIAIPGPVLDVYRLW RPTPLFRARRLERVLGTPARIYYKYEGVSPAGSHKPNTAVAQAYYNKQAGVKRLTTETGAGQWGSSLAMACAFFGMELKV YMVRASYLQKPYRRILMETWGAQCVPSPSPDTASGRRFYEQDPEHPGSLGIAISEAVEDAASRADTKYSLGSVLNHVLLH QTVIGLEALRQFELAGDYPDVIFGCVGGGSNFAGLTFPFVRDKLQGKDVRIVAVEPAACPSLTRGVYTYDFGDTARTTPL LKMHTLGHTFIPARIHAGGLRYHGMAPLVSHLYEHGLIEAIALPQTKVFEGAVLFARAEGIIPAPESAHAVRAAIDEARR CAETGEEKAILIGLSGHGHFDLAAYEAYLAGKLEDYEYPEEAVRQALAELPKVSG >Mature_454_residues SAAETVKFVLTEADLPTHWYNLAADLPTLPPPPIHPGTGEPVGPADLEPVFPMELILQEVSRERWIAIPGPVLDVYRLWR PTPLFRARRLERVLGTPARIYYKYEGVSPAGSHKPNTAVAQAYYNKQAGVKRLTTETGAGQWGSSLAMACAFFGMELKVY MVRASYLQKPYRRILMETWGAQCVPSPSPDTASGRRFYEQDPEHPGSLGIAISEAVEDAASRADTKYSLGSVLNHVLLHQ TVIGLEALRQFELAGDYPDVIFGCVGGGSNFAGLTFPFVRDKLQGKDVRIVAVEPAACPSLTRGVYTYDFGDTARTTPLL KMHTLGHTFIPARIHAGGLRYHGMAPLVSHLYEHGLIEAIALPQTKVFEGAVLFARAEGIIPAPESAHAVRAAIDEARRC AETGEEKAILIGLSGHGHFDLAAYEAYLAGKLEDYEYPEEAVRQALAELPKVSG
Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]
COG id: COG1350
COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the trpB family [H]
Homologues:
Organism=Escherichia coli, GI1787515, Length=361, Percent_Identity=29.3628808864266, Blast_Score=107, Evalue=1e-24, Organism=Saccharomyces cerevisiae, GI6321412, Length=372, Percent_Identity=30.9139784946237, Blast_Score=123, Evalue=6e-29,
Paralogues:
None
Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001926 - InterPro: IPR006316 - InterPro: IPR006653 - InterPro: IPR023026 [H]
Pfam domain/function: PF00291 PALP [H]
EC number: =4.2.1.20 [H]
Molecular weight: Translated: 49647; Mature: 49516
Theoretical pI: Translated: 6.46; Mature: 6.46
Prosite motif: PS00168 TRP_SYNTHASE_BETA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.1 %Cys (Translated Protein) 1.8 %Met (Translated Protein) 2.9 %Cys+Met (Translated Protein) 1.1 %Cys (Mature Protein) 1.5 %Met (Mature Protein) 2.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSAAETVKFVLTEADLPTHWYNLAADLPTLPPPPIHPGTGEPVGPADLEPVFPMELILQE CCHHHEEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH VSRERWIAIPGPVLDVYRLWRPTPLFRARRLERVLGTPARIYYKYEGVSPAGSHKPNTAV HCCCCEEECCCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHH AQAYYNKQAGVKRLTTETGAGQWGSSLAMACAFFGMELKVYMVRASYLQKPYRRILMETW HHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHCCHHEEEHHHHHHHHHHHHHHHHHHC GAQCVPSPSPDTASGRRFYEQDPEHPGSLGIAISEAVEDAASRADTKYSLGSVLNHVLLH CCCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH QTVIGLEALRQFELAGDYPDVIFGCVGGGSNFAGLTFPFVRDKLQGKDVRIVAVEPAACP HHHHHHHHHHHHHHCCCCHHHEEEECCCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCC SLTRGVYTYDFGDTARTTPLLKMHTLGHTFIPARIHAGGLRYHGMAPLVSHLYEHGLIEA HHHCCCEEEECCCCCCCCCEEEEHHCCCEEECCEEECCCCEECCHHHHHHHHHHCCCHHH IALPQTKVFEGAVLFARAEGIIPAPESAHAVRAAIDEARRCAETGEEKAILIGLSGHGHF HCCCCCHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCE DLAAYEAYLAGKLEDYEYPEEAVRQALAELPKVSG EHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCC >Mature Secondary Structure SAAETVKFVLTEADLPTHWYNLAADLPTLPPPPIHPGTGEPVGPADLEPVFPMELILQE CHHHEEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH VSRERWIAIPGPVLDVYRLWRPTPLFRARRLERVLGTPARIYYKYEGVSPAGSHKPNTAV HCCCCEEECCCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHH AQAYYNKQAGVKRLTTETGAGQWGSSLAMACAFFGMELKVYMVRASYLQKPYRRILMETW HHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHCCHHEEEHHHHHHHHHHHHHHHHHHC GAQCVPSPSPDTASGRRFYEQDPEHPGSLGIAISEAVEDAASRADTKYSLGSVLNHVLLH CCCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH QTVIGLEALRQFELAGDYPDVIFGCVGGGSNFAGLTFPFVRDKLQGKDVRIVAVEPAACP HHHHHHHHHHHHHHCCCCHHHEEEECCCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCC SLTRGVYTYDFGDTARTTPLLKMHTLGHTFIPARIHAGGLRYHGMAPLVSHLYEHGLIEA HHHCCCEEEECCCCCCCCCEEEEHHCCCEEECCEEECCCCEECCHHHHHHHHHHCCCHHH IALPQTKVFEGAVLFARAEGIIPAPESAHAVRAAIDEARRCAETGEEKAILIGLSGHGHF HCCCCCHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCE DLAAYEAYLAGKLEDYEYPEEAVRQALAELPKVSG EHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9537320 [H]