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The map label for this gene is fliM [H]

Identifier: 221635435

GI number: 221635435

Start: 25647

End: 26651

Strand: Reverse

Name: fliM [H]

Synonym: trd_A0029

Alternate gene names: 221635435

Gene position: 26651-25647 (Counterclockwise)

Preceding gene: 221635436

Following gene: 221635434

Centisome position: 2.9

GC content: 63.28

Gene sequence:

>1005_bases
ATGTCGCTCAGTCGTACCCGACCGACGACACGAGCCGCTCGCCGCGAACGCGGCAAGCCGATTCCCTACGATTTCCGTCG
TCCGGATAAGTTCTCCAAAGACCATATCCGGTCCATCCAGAGCATCCACGAAGCCTTCGACCGTTTCGCCTCCAACTATC
TCTCTGCCCAGGTCCGCTCGGCGGTTCACTGCGAACTGGGTCCAATCGAGCAGACGACGCTCGGCGATTACCTGGATCGC
CTGCCTTCACAGGTCGTCCTCTGCATAGCTGAGCTCGAGCCGCTCGTCGGCCGCATCGTTTTGGAAATCGACTTCGCGAC
TGCTTCGCGCCTCGTCGACCGCATGCTCGGGGGTGCCGGCGAGGAGCGCCCGCCTTTCACGGCGACGACGATCACCGAGA
TCGAGCTGCTGCTCCTCCGGGAACTCGGGAACGGCCTTTTCGGTGAGTTGGCAGCTGCGTGGGAACAGGTGATCCGTCTC
CAGCCTCAGCCGTGTGAAGTCGTCCTCTCCGGCCAGCAGATCCAGGGCATGATGCCGAGCGAGATCGTTTTGCTCGTCCA
GCACCAGGTGCGCTTGTTCGAAAGCGAGGGGACCTTCACGGTCGTCCTCCCCGCCTCGACGCTCGAGCCGGTCCTGCCCC
GTCTCAATGCTCGGGTCCTCTTTGCCAATCCCCGGCGCGCGACCTCTCCTCAGGTGATCGCCGATCTCATGGCGCAACTC
GAGGAAGTCTCGCTCCAGTTACGAGTGGAGCTCGGTCGCGCCCGCCTCACCGTGCGGGAACTGCTGGCACTGGAACCTGG
CGATGTCCTCGTGCTCGACCAGGACGTCTCGTTTCCCCTCGCAGTCTATGTCGAGAACGAGCCATGCTTCTATGCGTTTC
CTGGCCAGCGTGGGCGTTCCCTCGCGATACGCATCGCTGGGACGCTCACCGAGGATTGGCCACGCGACGAGGAGCTCGAT
CAGGAAGGAGGCACTGCCCTATGGCCGACCGTGACGACACCCTGA

Upstream 100 bases:

>100_bases
CTCAAGGACGAACTCCGCGAGAAGCTCCAGCCAGCTTTCGGTGACTACAAAATTACCGAGGTTTATCTCACGCAGTTCGT
GATCCAGTGAGGAGGCTCGC

Downstream 100 bases:

>100_bases
ACCGCGGCCAGGAGCCCAGCTCGGTTCGGCCTCCCGAAACCCCGATCCAGGAGGCGCGCTTCGTCGACCTGCGCGATCAG
CCGATCGGCAACGGTCGCCA

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MSLSRTRPTTRAARRERGKPIPYDFRRPDKFSKDHIRSIQSIHEAFDRFASNYLSAQVRSAVHCELGPIEQTTLGDYLDR
LPSQVVLCIAELEPLVGRIVLEIDFATASRLVDRMLGGAGEERPPFTATTITEIELLLLRELGNGLFGELAAAWEQVIRL
QPQPCEVVLSGQQIQGMMPSEIVLLVQHQVRLFESEGTFTVVLPASTLEPVLPRLNARVLFANPRRATSPQVIADLMAQL
EEVSLQLRVELGRARLTVRELLALEPGDVLVLDQDVSFPLAVYVENEPCFYAFPGQRGRSLAIRIAGTLTEDWPRDEELD
QEGGTALWPTVTTP

Sequences:

>Translated_334_residues
MSLSRTRPTTRAARRERGKPIPYDFRRPDKFSKDHIRSIQSIHEAFDRFASNYLSAQVRSAVHCELGPIEQTTLGDYLDR
LPSQVVLCIAELEPLVGRIVLEIDFATASRLVDRMLGGAGEERPPFTATTITEIELLLLRELGNGLFGELAAAWEQVIRL
QPQPCEVVLSGQQIQGMMPSEIVLLVQHQVRLFESEGTFTVVLPASTLEPVLPRLNARVLFANPRRATSPQVIADLMAQL
EEVSLQLRVELGRARLTVRELLALEPGDVLVLDQDVSFPLAVYVENEPCFYAFPGQRGRSLAIRIAGTLTEDWPRDEELD
QEGGTALWPTVTTP
>Mature_333_residues
SLSRTRPTTRAARRERGKPIPYDFRRPDKFSKDHIRSIQSIHEAFDRFASNYLSAQVRSAVHCELGPIEQTTLGDYLDRL
PSQVVLCIAELEPLVGRIVLEIDFATASRLVDRMLGGAGEERPPFTATTITEIELLLLRELGNGLFGELAAAWEQVIRLQ
PQPCEVVLSGQQIQGMMPSEIVLLVQHQVRLFESEGTFTVVLPASTLEPVLPRLNARVLFANPRRATSPQVIADLMAQLE
EVSLQLRVELGRARLTVRELLALEPGDVLVLDQDVSFPLAVYVENEPCFYAFPGQRGRSLAIRIAGTLTEDWPRDEELDQ
EGGTALWPTVTTP

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential). Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=309, Percent_Identity=22.3300970873786, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 37287; Mature: 37156

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSRTRPTTRAARRERGKPIPYDFRRPDKFSKDHIRSIQSIHEAFDRFASNYLSAQVRS
CCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVHCELGPIEQTTLGDYLDRLPSQVVLCIAELEPLVGRIVLEIDFATASRLVDRMLGGAG
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEHHHHHHHHHHHHCCCC
EERPPFTATTITEIELLLLRELGNGLFGELAAAWEQVIRLQPQPCEVVLSGQQIQGMMPS
CCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEECCCHHCCCCCH
EIVLLVQHQVRLFESEGTFTVVLPASTLEPVLPRLNARVLFANPRRATSPQVIADLMAQL
HHHHHHHHHHHHEECCCCEEEEEECHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHH
EEVSLQLRVELGRARLTVRELLALEPGDVLVLDQDVSFPLAVYVENEPCFYAFPGQRGRS
HHHHHHEEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEECCCCEEEECCCCCCCE
LAIRIAGTLTEDWPRDEELDQEGGTALWPTVTTP
EEEEEEEECCCCCCCCCHHHCCCCCEECCCCCCC
>Mature Secondary Structure 
SLSRTRPTTRAARRERGKPIPYDFRRPDKFSKDHIRSIQSIHEAFDRFASNYLSAQVRS
CCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVHCELGPIEQTTLGDYLDRLPSQVVLCIAELEPLVGRIVLEIDFATASRLVDRMLGGAG
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEHHHHHHHHHHHHCCCC
EERPPFTATTITEIELLLLRELGNGLFGELAAAWEQVIRLQPQPCEVVLSGQQIQGMMPS
CCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEECCCHHCCCCCH
EIVLLVQHQVRLFESEGTFTVVLPASTLEPVLPRLNARVLFANPRRATSPQVIADLMAQL
HHHHHHHHHHHHEECCCCEEEEEECHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHH
EEVSLQLRVELGRARLTVRELLALEPGDVLVLDQDVSFPLAVYVENEPCFYAFPGQRGRS
HHHHHHEEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEECCCCEEEECCCCCCCE
LAIRIAGTLTEDWPRDEELDQEGGTALWPTVTTP
EEEEEEEECCCCCCCCCHHHCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8755894; 9665876; 8529894 [H]