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The map label for this gene is nikB [C]

Identifier: 221633924

GI number: 221633924

Start: 1969011

End: 1970015

Strand: Reverse

Name: nikB [C]

Synonym: trd_1951

Alternate gene names: 221633924

Gene position: 1970015-1969011 (Counterclockwise)

Preceding gene: 221633925

Following gene: 221633923

Centisome position: 98.35

GC content: 55.92

Gene sequence:

>1005_bases
ATGCGCCTCCAATACGTGATGCGCCGGTTCCTACTCCTGTTCTTGGTCGTGTGGGTGGCGGCCACGCTGAACTTCTTCCT
CCCCCGCCTGGGAGGTCGCGATCCTATTCGCCAGAAACTCATGGAGCAAGCGGGTGCCGGGGGGTATGTCCATACCGGCA
TAGACGAAATGGTCCAAGAATACAACCGTAAATTTGGTTTAGACAAGCCGCTGTGGCAGCAGTACCTAACCTACCTCGGT
GACGTTTTCCGACTCGACCTCGGGTATTCGATCGCTCATTATCCACGTCGTGTCAGTGATGTCCTGGCCGAATCGCTTCC
CTGGACGATCGGTTTGCTCGCCACCACGACCATACTTGCGTTCGTGGTGGGCACTATTCTTGGTGCACTCCTGGGTTGGC
CCAGGAGTCCCCGTTTCGTCCAGTATCTGTTCCCCCCTCTCCTCACTCTCTCTGCGGTTCCGTACTTCCTCCTTGGCCTT
CTTCTCCTCTATCTCTTCGCCTTCCGCCTCAAGCTGCTGCCTCTGTTCGGTGGTTATACACCAGGCACTTTCCCGAGTTT
CTCGCTCAGCTTCGCGCTCGACGTCCTCCGACATTCCATCCTCCCGGCTGCTTCCATCCTCCTGGCTTCCCTCGGTTTCT
GGGCACTCGGCATGCGAGCCATGACTGTCACCGTCGAAGGGGAGGACTTCATGACTTTCGCTGAGGCGATCGGTCTGCGT
GACCGAACCCTCTTCTATCACTATGTGATTCGCAATGCGCTTCTTCCCCAGACGACTGCTCTGGGACTGGCCCTCGGCCA
AATCCTCTCCGGTGCACTGTTGGTTGAAGTCGTCTTCTCTTATCCTGGCATTGGAACCACTCTTTACCACTCGATCCGCA
CATTCGACTACTTTATGATTCAGGGCATCGTGTTCACAGTTGTTCTCGGAGTTACGTTCGCCACCTTTATCCTCGATCTT
CTTTATCCTCTGATCGACCCCCGTATCACCTATCAGGCGCGGTAA

Upstream 100 bases:

>100_bases
TTTCGCATCGCGCTGAGGACGAGCAGCTGCGCGGCTTGCTGCCCAGCGCGGTGTCCAATTGTTTTCTGAGGTACGGAGCT
GGTCACAAGGCTGGACAACC

Downstream 100 bases:

>100_bases
AGGAGGTCTTATGTGGGCATACCTCCGTCGTAATCCGAGCCTCTTGGTCGGTCTCATCTTGCTCGGCATCGTCCTCGCCT
TCGCCGGCATCGGCCGTCTC

Product: oligopeptide ABC transporter permease

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MRLQYVMRRFLLLFLVVWVAATLNFFLPRLGGRDPIRQKLMEQAGAGGYVHTGIDEMVQEYNRKFGLDKPLWQQYLTYLG
DVFRLDLGYSIAHYPRRVSDVLAESLPWTIGLLATTTILAFVVGTILGALLGWPRSPRFVQYLFPPLLTLSAVPYFLLGL
LLLYLFAFRLKLLPLFGGYTPGTFPSFSLSFALDVLRHSILPAASILLASLGFWALGMRAMTVTVEGEDFMTFAEAIGLR
DRTLFYHYVIRNALLPQTTALGLALGQILSGALLVEVVFSYPGIGTTLYHSIRTFDYFMIQGIVFTVVLGVTFATFILDL
LYPLIDPRITYQAR

Sequences:

>Translated_334_residues
MRLQYVMRRFLLLFLVVWVAATLNFFLPRLGGRDPIRQKLMEQAGAGGYVHTGIDEMVQEYNRKFGLDKPLWQQYLTYLG
DVFRLDLGYSIAHYPRRVSDVLAESLPWTIGLLATTTILAFVVGTILGALLGWPRSPRFVQYLFPPLLTLSAVPYFLLGL
LLLYLFAFRLKLLPLFGGYTPGTFPSFSLSFALDVLRHSILPAASILLASLGFWALGMRAMTVTVEGEDFMTFAEAIGLR
DRTLFYHYVIRNALLPQTTALGLALGQILSGALLVEVVFSYPGIGTTLYHSIRTFDYFMIQGIVFTVVLGVTFATFILDL
LYPLIDPRITYQAR
>Mature_334_residues
MRLQYVMRRFLLLFLVVWVAATLNFFLPRLGGRDPIRQKLMEQAGAGGYVHTGIDEMVQEYNRKFGLDKPLWQQYLTYLG
DVFRLDLGYSIAHYPRRVSDVLAESLPWTIGLLATTTILAFVVGTILGALLGWPRSPRFVQYLFPPLLTLSAVPYFLLGL
LLLYLFAFRLKLLPLFGGYTPGTFPSFSLSFALDVLRHSILPAASILLASLGFWALGMRAMTVTVEGEDFMTFAEAIGLR
DRTLFYHYVIRNALLPQTTALGLALGQILSGALLVEVVFSYPGIGTTLYHSIRTFDYFMIQGIVFTVVLGVTFATFILDL
LYPLIDPRITYQAR

Specific function: Probably part of an ABC transporter complex that could be involved in peptide import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789888, Length=339, Percent_Identity=31.858407079646, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1789965, Length=347, Percent_Identity=29.6829971181556, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1787053, Length=336, Percent_Identity=28.2738095238095, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787761, Length=337, Percent_Identity=32.9376854599407, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787497, Length=331, Percent_Identity=26.5861027190332, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1788504, Length=365, Percent_Identity=26.5753424657534, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1787550, Length=275, Percent_Identity=27.6363636363636, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 37607; Mature: 37607

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLQYVMRRFLLLFLVVWVAATLNFFLPRLGGRDPIRQKLMEQAGAGGYVHTGIDEMVQE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHH
YNRKFGLDKPLWQQYLTYLGDVFRLDLGYSIAHYPRRVSDVLAESLPWTIGLLATTTILA
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
FVVGTILGALLGWPRSPRFVQYLFPPLLTLSAVPYFLLGLLLLYLFAFRLKLLPLFGGYT
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PGTFPSFSLSFALDVLRHSILPAASILLASLGFWALGMRAMTVTVEGEDFMTFAEAIGLR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHCCC
DRTLFYHYVIRNALLPQTTALGLALGQILSGALLVEVVFSYPGIGTTLYHSIRTFDYFMI
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
QGIVFTVVLGVTFATFILDLLYPLIDPRITYQAR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRLQYVMRRFLLLFLVVWVAATLNFFLPRLGGRDPIRQKLMEQAGAGGYVHTGIDEMVQE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHH
YNRKFGLDKPLWQQYLTYLGDVFRLDLGYSIAHYPRRVSDVLAESLPWTIGLLATTTILA
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
FVVGTILGALLGWPRSPRFVQYLFPPLLTLSAVPYFLLGLLLLYLFAFRLKLLPLFGGYT
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PGTFPSFSLSFALDVLRHSILPAASILLASLGFWALGMRAMTVTVEGEDFMTFAEAIGLR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHCCC
DRTLFYHYVIRNALLPQTTALGLALGQILSGALLVEVVFSYPGIGTTLYHSIRTFDYFMI
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
QGIVFTVVLGVTFATFILDLLYPLIDPRITYQAR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA