Click here to switch to the map view.

The map label for this gene is yomI [H]

Identifier: 221633509

GI number: 221633509

Start: 1512523

End: 1514769

Strand: Reverse

Name: yomI [H]

Synonym: trd_1531

Alternate gene names: 221633509

Gene position: 1514769-1512523 (Counterclockwise)

Preceding gene: 221633515

Following gene: 221633507

Centisome position: 75.62

GC content: 68.45

Gene sequence:

>2247_bases
ATGGGCGGGGTACGCCGCGGACTGCTACACAGCGGACTGCTCTCGCTCGCAGTACTCGTCGTCCTCGCGACCGCACCACT
GTCGAGCGAGCACTCCTCGAGCCTGCCGATCGTCGGGAATCAGGCCGGGAACGCCGCCGAACCGGGTGAGGACGACGCGC
TGCGGACCCCAGACGACCCGGTCGAGGCGCTCCTCCAAGCGGAGCGACTTTTGGCAGCGGGATCGTCTGCGCTGGCTGCC
CGGGTCGTCGCGCCAGTCTTGGGGGCGAGCGATCCGGTGTTGCGGGTCCGGGCGCGCTTGCTTTTGGCTCGCGCGATGCT
CGCTGGTGGGGATGCTGCGGCAGCGCTGGAACTCGCTGCGGCCGTGGCACGACAAGCTTCCGCGACGCGGGAGACGGGGT
TGGCACTCGTGATCGCCGGGGAGGCGCTCCTGGCGCGTGGCCAGCCTGGCCAAGCAGCCGAGGAGTTCCTGGCTGCACGC
GCGTTGTTGCCTGAGATCGCGCCCTATCTCGAGTATCGTGCGCTCGATCCGCTCGTGCAGGCCGGTCGCGCGCACGAGGC
GGAGGCGTTGGTCGAGCAGATCGTTGCGACAGCACCGCTCCAGCGGCTCGCGGTGGCCGCTCTGGAATCGCGGCGTGCCT
GGGCAGAACAGCGCGGTGATCTCCCAACGCTCGCCGCGACGCTCGACCGACTGCTCGATCTGGCGACGATCCCCAGTTAC
CGCGCTTCGCTTTTGCTCCAGCGCGGTCGTGTCGAGCAGCAGCGTGGGGATGCGGCAGCCGCGCGAGCCGACTTCGCACG
GGTGATCGACGAGGCACCGGCCAGCCCGGCTGCGGCGCTCGCGCTCGATGAGCTGGCTGCGCTCGGGGTGGCAGAGACGG
TGCCGATCGAGCGACGGGCTGCGATCCTCTTCTCAGCGGGGCGATATCGGGAGGCGGTGACTGCCTTCACTGCTCTCCTG
GCGAGCGATCCGACGCGAGCCGAGGCATGGTACCAGCGGGCGATCGCGCGGGTGCGCGCGGGCGACCTCGCCGCAGGGAT
CGAAGAACTCACCGCGATGGCTGACCGTTACCCCCAAGACGCACGAGCACCGGATGCCCTGGTGACTGCCGCAATGCTCC
TCGAGTGGAGGAACGAGCCGGCGGCTGAGGTGCTGTACCGCCGCGTCCTGTCACAGTATCCAGAGACTGTGGCAGCCCGT
CAGGCCCGCTTCCGTCTCGGCCTCCTCGCCTTTGCGCGTGGCGCGCACGACGAGGCCCGCGATCTCTGGCGAGCGGATGC
CGAGGCGGGCGATCCCCGCGCCGCCTACTGGTATGGAAAGGCGCTGGCAGCGAGCGGTGAGCCAGTGGGAGCGCGCGCAT
GGTGGTCCCGCGCCTACCAGCGCGATCCGAACAGCTTTTGGGGACAGCGGGCCGCGGATCTCCTGTCCGGCCGCACCACG
ATTCTGCAGCAGCCGGTCGCACCACTTTCGCTCGACTCGGAGGCTGATGTGGTTCTCGCTTGGCTGGACGGCAGAGGAGA
AAATCTCGCTGCGGCCAGGGAGGAAATCGAGAAAACCGCTGCGCCCCGGCGGGCTCTCTTGCTCCTCGATGCCGGGCTGC
GTGAAGCTGCCGGCTGGGAGGTCGATGCTGCCCGGACGGAGTTGCGGGATCGGCCCGTGTCGCTCGCTTTCTTCGGCTGG
CTTCTCCTGGAGCGTGGTGAGGCAGCCTATGCCTATCGCATCGGAGGAGACCTGGCGACCGACGAACGAGTACCCGAGCA
GGTCCGTGCCCGTCTCCTGGCGCCGATACCCTATCCGGAAGTGCTCGTTGCGGCGAGCGAGCGTTTTGGAATCGATCCGT
TGCTGCTCGCTGCCCTGATCCGGCAGGAGAGCGGGTTTGAGCCGACTGCCGTCTCGCCGGCCGGTGCCCGCGGACTGACG
CAGGTCATGCCGGAAACGGCAGCGGCCCTGGCGCGGCAGCTCGGCCTGTCCAGTTGGGATCCAGCGGATTCCTTCCGTCC
GGAAACGAGTATCATGCTCGGTGCAGCGGAGCTCGCCCAGCGCTTGCGGCAATTCCACGGGGACCTCGTTCTCACCCTCG
CGAGCTACAACGCAGGCCCCGGAGCCGTCCAACAATGGCTCGCTGAACGACCGACCAGCGACCCTGATCTCTTCGTGGCT
TGGATTCCGTACCGGGAAACGGCCGAGTACGTCCAACGCGTCTACGCTGGCTATCGCCGGTACCACGAGTTGGCCCGTCT
CGGCTGA

Upstream 100 bases:

>100_bases
TGCGGTAACCGGAAGGTAAACGGAGGGACCGACGTCGTGTCTCCCGAACCGTTTCCGTCTATCCTGAAACCAGGGCTTGC
TGAGTCGGGTGGAACATGCC

Downstream 100 bases:

>100_bases
CGAGCGAGCCGGCGTGTTGCCAGTCGCACGGCGTGGGGGATCGCCGGTCGACGGATGTCCTCGGCTCTCGAGGCTTTCCT
CGCTCGGCTGGTTCTCCCAC

Product: putative soluble lytic murein transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 748; Mature: 747

Protein sequence:

>748_residues
MGGVRRGLLHSGLLSLAVLVVLATAPLSSEHSSSLPIVGNQAGNAAEPGEDDALRTPDDPVEALLQAERLLAAGSSALAA
RVVAPVLGASDPVLRVRARLLLARAMLAGGDAAAALELAAAVARQASATRETGLALVIAGEALLARGQPGQAAEEFLAAR
ALLPEIAPYLEYRALDPLVQAGRAHEAEALVEQIVATAPLQRLAVAALESRRAWAEQRGDLPTLAATLDRLLDLATIPSY
RASLLLQRGRVEQQRGDAAAARADFARVIDEAPASPAAALALDELAALGVAETVPIERRAAILFSAGRYREAVTAFTALL
ASDPTRAEAWYQRAIARVRAGDLAAGIEELTAMADRYPQDARAPDALVTAAMLLEWRNEPAAEVLYRRVLSQYPETVAAR
QARFRLGLLAFARGAHDEARDLWRADAEAGDPRAAYWYGKALAASGEPVGARAWWSRAYQRDPNSFWGQRAADLLSGRTT
ILQQPVAPLSLDSEADVVLAWLDGRGENLAAAREEIEKTAAPRRALLLLDAGLREAAGWEVDAARTELRDRPVSLAFFGW
LLLERGEAAYAYRIGGDLATDERVPEQVRARLLAPIPYPEVLVAASERFGIDPLLLAALIRQESGFEPTAVSPAGARGLT
QVMPETAAALARQLGLSSWDPADSFRPETSIMLGAAELAQRLRQFHGDLVLTLASYNAGPGAVQQWLAERPTSDPDLFVA
WIPYRETAEYVQRVYAGYRRYHELARLG

Sequences:

>Translated_748_residues
MGGVRRGLLHSGLLSLAVLVVLATAPLSSEHSSSLPIVGNQAGNAAEPGEDDALRTPDDPVEALLQAERLLAAGSSALAA
RVVAPVLGASDPVLRVRARLLLARAMLAGGDAAAALELAAAVARQASATRETGLALVIAGEALLARGQPGQAAEEFLAAR
ALLPEIAPYLEYRALDPLVQAGRAHEAEALVEQIVATAPLQRLAVAALESRRAWAEQRGDLPTLAATLDRLLDLATIPSY
RASLLLQRGRVEQQRGDAAAARADFARVIDEAPASPAAALALDELAALGVAETVPIERRAAILFSAGRYREAVTAFTALL
ASDPTRAEAWYQRAIARVRAGDLAAGIEELTAMADRYPQDARAPDALVTAAMLLEWRNEPAAEVLYRRVLSQYPETVAAR
QARFRLGLLAFARGAHDEARDLWRADAEAGDPRAAYWYGKALAASGEPVGARAWWSRAYQRDPNSFWGQRAADLLSGRTT
ILQQPVAPLSLDSEADVVLAWLDGRGENLAAAREEIEKTAAPRRALLLLDAGLREAAGWEVDAARTELRDRPVSLAFFGW
LLLERGEAAYAYRIGGDLATDERVPEQVRARLLAPIPYPEVLVAASERFGIDPLLLAALIRQESGFEPTAVSPAGARGLT
QVMPETAAALARQLGLSSWDPADSFRPETSIMLGAAELAQRLRQFHGDLVLTLASYNAGPGAVQQWLAERPTSDPDLFVA
WIPYRETAEYVQRVYAGYRRYHELARLG
>Mature_747_residues
GGVRRGLLHSGLLSLAVLVVLATAPLSSEHSSSLPIVGNQAGNAAEPGEDDALRTPDDPVEALLQAERLLAAGSSALAAR
VVAPVLGASDPVLRVRARLLLARAMLAGGDAAAALELAAAVARQASATRETGLALVIAGEALLARGQPGQAAEEFLAARA
LLPEIAPYLEYRALDPLVQAGRAHEAEALVEQIVATAPLQRLAVAALESRRAWAEQRGDLPTLAATLDRLLDLATIPSYR
ASLLLQRGRVEQQRGDAAAARADFARVIDEAPASPAAALALDELAALGVAETVPIERRAAILFSAGRYREAVTAFTALLA
SDPTRAEAWYQRAIARVRAGDLAAGIEELTAMADRYPQDARAPDALVTAAMLLEWRNEPAAEVLYRRVLSQYPETVAARQ
ARFRLGLLAFARGAHDEARDLWRADAEAGDPRAAYWYGKALAASGEPVGARAWWSRAYQRDPNSFWGQRAADLLSGRTTI
LQQPVAPLSLDSEADVVLAWLDGRGENLAAAREEIEKTAAPRRALLLLDAGLREAAGWEVDAARTELRDRPVSLAFFGWL
LLERGEAAYAYRIGGDLATDERVPEQVRARLLAPIPYPEVLVAASERFGIDPLLLAALIRQESGFEPTAVSPAGARGLTQ
VMPETAAALARQLGLSSWDPADSFRPETSIMLGAAELAQRLRQFHGDLVLTLASYNAGPGAVQQWLAERPTSDPDLFVAW
IPYRETAEYVQRVYAGYRRYHELARLG

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 10 TPR repeats [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=152, Percent_Identity=34.8684210526316, Blast_Score=88, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR008258
- InterPro:   IPR016047
- InterPro:   IPR010090
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01551 Peptidase_M23; PF10145 PhageMin_Tail; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 80192; Mature: 80061

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50293 TPR_REGION L=RR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGVRRGLLHSGLLSLAVLVVLATAPLSSEHSSSLPIVGNQAGNAAEPGEDDALRTPDDP
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCHH
VEALLQAERLLAAGSSALAARVVAPVLGASDPVLRVRARLLLARAMLAGGDAAAALELAA
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
AVARQASATRETGLALVIAGEALLARGQPGQAAEEFLAARALLPEIAPYLEYRALDPLVQ
HHHHHHHHHHHCCEEEEEECHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGRAHEAEALVEQIVATAPLQRLAVAALESRRAWAEQRGDLPTLAATLDRLLDLATIPSY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHH
RASLLLQRGRVEQQRGDAAAARADFARVIDEAPASPAAALALDELAALGVAETVPIERRA
HHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHCCCHHHHH
AILFSAGRYREAVTAFTALLASDPTRAEAWYQRAIARVRAGDLAAGIEELTAMADRYPQD
HHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ARAPDALVTAAMLLEWRNEPAAEVLYRRVLSQYPETVAARQARFRLGLLAFARGAHDEAR
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHH
DLWRADAEAGDPRAAYWYGKALAASGEPVGARAWWSRAYQRDPNSFWGQRAADLLSGRTT
HHHHHCCCCCCCCEEEEECHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHH
ILQQPVAPLSLDSEADVVLAWLDGRGENLAAAREEIEKTAAPRRALLLLDAGLREAAGWE
HHHCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCHHEEEEECCCCHHHCCCC
VDAARTELRDRPVSLAFFGWLLLERGEAAYAYRIGGDLATDERVPEQVRARLLAPIPYPE
CHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCCCHH
VLVAASERFGIDPLLLAALIRQESGFEPTAVSPAGARGLTQVMPETAAALARQLGLSSWD
HHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
PADSFRPETSIMLGAAELAQRLRQFHGDLVLTLASYNAGPGAVQQWLAERPTSDPDLFVA
CCCCCCCCHHHEECHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEE
WIPYRETAEYVQRVYAGYRRYHELARLG
ECCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GGVRRGLLHSGLLSLAVLVVLATAPLSSEHSSSLPIVGNQAGNAAEPGEDDALRTPDDP
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCHH
VEALLQAERLLAAGSSALAARVVAPVLGASDPVLRVRARLLLARAMLAGGDAAAALELAA
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
AVARQASATRETGLALVIAGEALLARGQPGQAAEEFLAARALLPEIAPYLEYRALDPLVQ
HHHHHHHHHHHCCEEEEEECHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGRAHEAEALVEQIVATAPLQRLAVAALESRRAWAEQRGDLPTLAATLDRLLDLATIPSY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHH
RASLLLQRGRVEQQRGDAAAARADFARVIDEAPASPAAALALDELAALGVAETVPIERRA
HHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHCCCHHHHH
AILFSAGRYREAVTAFTALLASDPTRAEAWYQRAIARVRAGDLAAGIEELTAMADRYPQD
HHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ARAPDALVTAAMLLEWRNEPAAEVLYRRVLSQYPETVAARQARFRLGLLAFARGAHDEAR
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHH
DLWRADAEAGDPRAAYWYGKALAASGEPVGARAWWSRAYQRDPNSFWGQRAADLLSGRTT
HHHHHCCCCCCCCEEEEECHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHH
ILQQPVAPLSLDSEADVVLAWLDGRGENLAAAREEIEKTAAPRRALLLLDAGLREAAGWE
HHHCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCHHEEEEECCCCHHHCCCC
VDAARTELRDRPVSLAFFGWLLLERGEAAYAYRIGGDLATDERVPEQVRARLLAPIPYPE
CHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCCCHH
VLVAASERFGIDPLLLAALIRQESGFEPTAVSPAGARGLTQVMPETAAALARQLGLSSWD
HHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
PADSFRPETSIMLGAAELAQRLRQFHGDLVLTLASYNAGPGAVQQWLAERPTSDPDLFVA
CCCCCCCCHHHEECHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEE
WIPYRETAEYVQRVYAGYRRYHELARLG
ECCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]