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The map label for this gene is lysX [H]

Identifier: 221633497

GI number: 221633497

Start: 1499443

End: 1500327

Strand: Reverse

Name: lysX [H]

Synonym: trd_1519

Alternate gene names: 221633497

Gene position: 1500327-1499443 (Counterclockwise)

Preceding gene: 221633498

Following gene: 221633496

Centisome position: 74.9

GC content: 65.2

Gene sequence:

>885_bases
GTGAGTGCACCGCGGATCGGTGTCTTGCTCTCGCATGTCCGGGAAGAGGAGAAGCTGCTCCTGCGTGCGCTCAGCGAGCG
GGGAGCCGAGGTCGTGCGCCTCTACGACCGGCAGCTGGTCTTCGATTTGACGGACGAGCGGAGCTGGCCCCAGGTCGATC
TGGTACTCGATCGCTGCATGGCGCATAGCCGGGGGCAGGTCGCGCTACGCCTCTTCGAGTCGGCTGGCGTCCCGACGATC
AATCGGAGCCAGGCGACGGCGATCGCTGACGACAAGGTGCTGACCACGCGGTTGCTCGCAGCAGCTGGTATCCCGACCCT
CCGCACGCTCGTCGCCTTCGACGTCGAGAGTGCGCTGGCAGCGCTGGAACGGCTCGGTTATCCAGCTGTCATCAAGCCGG
TGACCGGTTCCTGGGGACGGCTCCTCGCTCGCGTCAACTCACCAGCGGCAGCCCGCGCCGTGCTGCAGCACAAGCGGTCG
CTCGGCTCTTTCCACCACGGAGTCTTCTATCTCCAGGAATACGTGGACAAGCCGGGCCGCGACGTGCGCGTCTTCGTGGT
CGGTGACCGGGTGGTGGCTGCTTCCTATCGTCAGGCCGACCACTGGGTGACCAATGTCGCTCGCGGGGCGGTTTCTTATC
CCTGCCCTGTGACACCTGAACTCGCCGATCTCGCCTTGCGGGCTGTCCAGACGATCGGTTTGGAGATCGCCGGAGTCGAT
CTCGTCGAGACTGCCGACGGACTCGAGGTGCTGGAAGTCAATGGTGGTGTCGAGTTCAAGGGCCTGATGCGGACGACTCA
TATCGATGTCGCCGGTCTCATCGCCGATTACGTGCTCGCGCGGGCGACGCGAGGTGCGTCGCCCGCGATCGCATTGAGCC
GGTGA

Upstream 100 bases:

>100_bases
ATATCGCGATCGAGGAGTTCCTGCGCGGGATCATGGTCCTAGAGCGGGTGATCGAACGCGTGGCAGCCCAGATCGTGGGT
GGGCGATGGGGGGACGAGTC

Downstream 100 bases:

>100_bases
AACGTTCGCCGACGGGAGGGAGAGGCGGTCACGGATGGTCGTATCGGTCGCGATCTTGGGGGGCTCTGGGTATACGGGTG
GTGAATTGCTTCGCTTGCTG

Product: Lysine biosynthesis protein LysX

Products: NA

Alternate protein names: AAA--lysW ligase lysX [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MSAPRIGVLLSHVREEEKLLLRALSERGAEVVRLYDRQLVFDLTDERSWPQVDLVLDRCMAHSRGQVALRLFESAGVPTI
NRSQATAIADDKVLTTRLLAAAGIPTLRTLVAFDVESALAALERLGYPAVIKPVTGSWGRLLARVNSPAAARAVLQHKRS
LGSFHHGVFYLQEYVDKPGRDVRVFVVGDRVVAASYRQADHWVTNVARGAVSYPCPVTPELADLALRAVQTIGLEIAGVD
LVETADGLEVLEVNGGVEFKGLMRTTHIDVAGLIADYVLARATRGASPAIALSR

Sequences:

>Translated_294_residues
MSAPRIGVLLSHVREEEKLLLRALSERGAEVVRLYDRQLVFDLTDERSWPQVDLVLDRCMAHSRGQVALRLFESAGVPTI
NRSQATAIADDKVLTTRLLAAAGIPTLRTLVAFDVESALAALERLGYPAVIKPVTGSWGRLLARVNSPAAARAVLQHKRS
LGSFHHGVFYLQEYVDKPGRDVRVFVVGDRVVAASYRQADHWVTNVARGAVSYPCPVTPELADLALRAVQTIGLEIAGVD
LVETADGLEVLEVNGGVEFKGLMRTTHIDVAGLIADYVLARATRGASPAIALSR
>Mature_293_residues
SAPRIGVLLSHVREEEKLLLRALSERGAEVVRLYDRQLVFDLTDERSWPQVDLVLDRCMAHSRGQVALRLFESAGVPTIN
RSQATAIADDKVLTTRLLAAAGIPTLRTLVAFDVESALAALERLGYPAVIKPVTGSWGRLLARVNSPAAARAVLQHKRSL
GSFHHGVFYLQEYVDKPGRDVRVFVVGDRVVAASYRQADHWVTNVARGAVSYPCPVTPELADLALRAVQTIGLEIAGVDL
VETADGLEVLEVNGGVEFKGLMRTTHIDVAGLIADYVLARATRGASPAIALSR

Specific function: Catalyzes the ATP-dependent binding between the amino group of alpha-aminoadipate (AAA) and the gamma-carboxyl group of 'Glu-54' of lysW [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI164663810, Length=222, Percent_Identity=31.5315315315315, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI134254462, Length=219, Percent_Identity=31.9634703196347, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1787076, Length=243, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011870
- InterPro:   IPR016185
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 31888; Mature: 31757

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPRIGVLLSHVREEEKLLLRALSERGAEVVRLYDRQLVFDLTDERSWPQVDLVLDRCM
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHH
AHSRGQVALRLFESAGVPTINRSQATAIADDKVLTTRLLAAAGIPTLRTLVAFDVESALA
HCCCCHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ALERLGYPAVIKPVTGSWGRLLARVNSPAAARAVLQHKRSLGSFHHGVFYLQEYVDKPGR
HHHHCCCCEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DVRVFVVGDRVVAASYRQADHWVTNVARGAVSYPCPVTPELADLALRAVQTIGLEIAGVD
EEEEEEECCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCEEECCC
LVETADGLEVLEVNGGVEFKGLMRTTHIDVAGLIADYVLARATRGASPAIALSR
CEECCCCCEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCC
>Mature Secondary Structure 
SAPRIGVLLSHVREEEKLLLRALSERGAEVVRLYDRQLVFDLTDERSWPQVDLVLDRCM
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHH
AHSRGQVALRLFESAGVPTINRSQATAIADDKVLTTRLLAAAGIPTLRTLVAFDVESALA
HCCCCHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ALERLGYPAVIKPVTGSWGRLLARVNSPAAARAVLQHKRSLGSFHHGVFYLQEYVDKPGR
HHHHCCCCEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DVRVFVVGDRVVAASYRQADHWVTNVARGAVSYPCPVTPELADLALRAVQTIGLEIAGVD
EEEEEEECCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCEEECCC
LVETADGLEVLEVNGGVEFKGLMRTTHIDVAGLIADYVLARATRGASPAIALSR
CEECCCCCEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12963379 [H]