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The map label for this gene is metE [H]

Identifier: 221633329

GI number: 221633329

Start: 1341376

End: 1343667

Strand: Reverse

Name: metE [H]

Synonym: trd_1349

Alternate gene names: 221633329

Gene position: 1343667-1341376 (Counterclockwise)

Preceding gene: 221633330

Following gene: 221633328

Centisome position: 67.08

GC content: 64.4

Gene sequence:

>2292_bases
ATGGCCCAGAGCTCGGTCCTCGGCTATCCCCGAATCGGCAAGCAGCGTGAACTGAAGCGGGCCCTCGAGCGTTACTGGGC
CGGTGAGGCTGATGCAGCGGCCTTGCAGGAGACGGCTCGAGCAGTCCGCGAAGCGGCGTGGCGTGTGCAGTTCCAGGCCG
GGATCGATCAGATTCCCTGTAACGATTTCAGCCTCTACGACCATGTCCTGGATACGATCTGTCTGGTCGGGCTCGTGCCG
TCCCGGTTCGGCTCGGCCGAGCCAGAAGTCAGTCTGCCGACCTACTTCGCCATGGCGCGGGGGGCACCGGGTGTTCCAGC
GCTCGAGTTGACGAAGTGGTTCGATACCAACTACCACTATCTCGTACCGGAATTCGATGCGCCTGGTCCGCGTCTGGCCA
GCGCCAAGCCCTTCCTCGAGTTCGCCGAGGCGACGTCGCTCTTGGGACGACCGGCCAAGCCGGTTCTGCTGGGCCCGATT
TCGCTTTTGCTCCTCGGCAAGTATCACCAGCGGCCGTTGCGCGAGCACCTGGAGCAATTGCTCCCGATCTACCAGGAGGT
CTTGGAGCGGCTCGCACGGGCTGGTGCCGAGTGGGTCCAGATGGACGAGCCGGTTCTCGTGCAGGATCGCAGCGACGAAG
AGTTGCAGCTGCTCCGGGCAGCCTATCGCGTGCTCGGCGAAGCGAGCGCTCGTCCGAAGCTGATGCTGCAGACCTACTAT
GGCAGTCTCGACGATGCGAGTTGGGACGTCGTCATGAGTCTGCCGATCGAAGGGATCGGTCTGGACTTCGTGCGGGACGA
CGGTAACCGAGCTTTGCTCAACCGGCACGGCTTCCCCCAGGGGAAGGTGATCGGTGCGGGAGTCGTGAATGGGCGCAACA
TCTGGCGAACGAATCTTGTGGAAGCTCTCGAGAAGCTGGAAGAGATCGCCGAGCATGTGGAGCCGGAGCGTATCTGGGTC
CAGCCGTCCTGCACGCTCTTGCATCTCCCGCACGACGTGCGACTGGAAACCAAGCTCCCGGCTGCAGTGCGCGCATTGCT
GGCGTTCGCTGAGCAACGACTGGAAGAGTTGGTGATCTTGACGCGTGCACTCAACGAGGGACGTGCAGCCATTCTGGAAG
AGCTCGAGCAGAACGCGGAGGCGTTTCGCCTGGCGCTGGACGAGGTTCGGGTCTACCGGCGCGACGTGCGCGAGCGGCTG
GCTGCGCTCACCGAGGACGACTTCCGGCGCCAGTCGCCGTACGAGATGCGCGAACCGCTCCAGCGGGCGCGCTTCCACCT
TCCCCTCTTTCCGACGACGACGATCGGCAGCTTTCCGCAAACGCCGGACGTCCGCCAGGCCCGTGCACGCTGGCGTGCCG
GCAAGCTCTCGGATGAGGACTATTGGGGGTTCATCCGCGAGCGGATCGCCGAGGTCATCCGGCGTCAGGAGGAGATCGGG
CTGGATGTCCTGGTGCACGGCGAGTACGAGCGTGCCGACATGGTGGAGTACTTCGCCGAGCAACTGGAAGGATTCGTCCT
GACCGAACATGGCTGGGTGCAGTCGTACGGGAGCCGCGTGGTCAGACCGCCGATCATCTTCGGTGATGTGGCGCGACCAC
GACCGATGACAGTTGAAGTGAGTCGGTGTGCGCAGTCGCTGACCGAGAAACCGGTCAAAGGCATGCTGACCGGTCCGGTG
ACGATCCTGAACTGGTCGTTCGTCCGGGAAGATATCCCGCGACGCGAGGTCGCGTACCAGATCGCGCTGGCGCTGCGCGA
CGAGGTTCGTGACCTGGAGGCTGCCGGGATCAGCATGATCCAGATCGACGAGCCGGCGCTCCGTGAGGGGCTTCCCTTGC
GTCGAGCAGACTGGCCGGAGTACCTCGAGTGGGCCGTGCGCGCCTTCCGCCTGGCGAGTTCGGGCGTTCGTGACGACACG
CAAATCCACTCGCACATGTGCTACAGCGAGTTCAACGACATCATCGAGGCGATCGACGCGCTCGACGCGGACGTCATCTC
GATCGAGAACAGTCGGAGTCGGGGAGAGCTCCTGCAGGCGTTCCAGGAGTACCGCTACCCGCGGCAGATTGGTCCGGGCG
TCTACGACGTCCACTCCGCGCGGATCCCGAGCGTCGAGGAGATGCTCGAGCTGCTGGAGCGTGCGCTCGCATTCCTGCGA
CCGGAGCAGCTGTGGGTCAATCCGGACTGCGGACTCAAGACCCGGCGTCCGGAGGAGGTTTGGCCATCGCTCAGCAACAT
GGTCGAAGCCGCGCGCCGACTCCGCGCGCGCTTCACAGCGCAGGGCCAGTGA

Upstream 100 bases:

>100_bases
TGCCCTTTGACGATGCGCTCATCGCGTGTTATCGTCGCCGAGCGAGTCGTGATGAGCGATCGCAAGCCTGAGTGAACCGA
ACAGAGGAGAGAGGGAGCGC

Downstream 100 bases:

>100_bases
AGCTGAGGAACTGCTCACGGGGGCGATGATGGGCCAGGCGGTCGATACGCGTTCGCTTCCCGTCATGCCGTTCGGCCCAT
TCACCTTGCGGCCGTTCGCC

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 763; Mature: 762

Protein sequence:

>763_residues
MAQSSVLGYPRIGKQRELKRALERYWAGEADAAALQETARAVREAAWRVQFQAGIDQIPCNDFSLYDHVLDTICLVGLVP
SRFGSAEPEVSLPTYFAMARGAPGVPALELTKWFDTNYHYLVPEFDAPGPRLASAKPFLEFAEATSLLGRPAKPVLLGPI
SLLLLGKYHQRPLREHLEQLLPIYQEVLERLARAGAEWVQMDEPVLVQDRSDEELQLLRAAYRVLGEASARPKLMLQTYY
GSLDDASWDVVMSLPIEGIGLDFVRDDGNRALLNRHGFPQGKVIGAGVVNGRNIWRTNLVEALEKLEEIAEHVEPERIWV
QPSCTLLHLPHDVRLETKLPAAVRALLAFAEQRLEELVILTRALNEGRAAILEELEQNAEAFRLALDEVRVYRRDVRERL
AALTEDDFRRQSPYEMREPLQRARFHLPLFPTTTIGSFPQTPDVRQARARWRAGKLSDEDYWGFIRERIAEVIRRQEEIG
LDVLVHGEYERADMVEYFAEQLEGFVLTEHGWVQSYGSRVVRPPIIFGDVARPRPMTVEVSRCAQSLTEKPVKGMLTGPV
TILNWSFVREDIPRREVAYQIALALRDEVRDLEAAGISMIQIDEPALREGLPLRRADWPEYLEWAVRAFRLASSGVRDDT
QIHSHMCYSEFNDIIEAIDALDADVISIENSRSRGELLQAFQEYRYPRQIGPGVYDVHSARIPSVEEMLELLERALAFLR
PEQLWVNPDCGLKTRRPEEVWPSLSNMVEAARRLRARFTAQGQ

Sequences:

>Translated_763_residues
MAQSSVLGYPRIGKQRELKRALERYWAGEADAAALQETARAVREAAWRVQFQAGIDQIPCNDFSLYDHVLDTICLVGLVP
SRFGSAEPEVSLPTYFAMARGAPGVPALELTKWFDTNYHYLVPEFDAPGPRLASAKPFLEFAEATSLLGRPAKPVLLGPI
SLLLLGKYHQRPLREHLEQLLPIYQEVLERLARAGAEWVQMDEPVLVQDRSDEELQLLRAAYRVLGEASARPKLMLQTYY
GSLDDASWDVVMSLPIEGIGLDFVRDDGNRALLNRHGFPQGKVIGAGVVNGRNIWRTNLVEALEKLEEIAEHVEPERIWV
QPSCTLLHLPHDVRLETKLPAAVRALLAFAEQRLEELVILTRALNEGRAAILEELEQNAEAFRLALDEVRVYRRDVRERL
AALTEDDFRRQSPYEMREPLQRARFHLPLFPTTTIGSFPQTPDVRQARARWRAGKLSDEDYWGFIRERIAEVIRRQEEIG
LDVLVHGEYERADMVEYFAEQLEGFVLTEHGWVQSYGSRVVRPPIIFGDVARPRPMTVEVSRCAQSLTEKPVKGMLTGPV
TILNWSFVREDIPRREVAYQIALALRDEVRDLEAAGISMIQIDEPALREGLPLRRADWPEYLEWAVRAFRLASSGVRDDT
QIHSHMCYSEFNDIIEAIDALDADVISIENSRSRGELLQAFQEYRYPRQIGPGVYDVHSARIPSVEEMLELLERALAFLR
PEQLWVNPDCGLKTRRPEEVWPSLSNMVEAARRLRARFTAQGQ
>Mature_762_residues
AQSSVLGYPRIGKQRELKRALERYWAGEADAAALQETARAVREAAWRVQFQAGIDQIPCNDFSLYDHVLDTICLVGLVPS
RFGSAEPEVSLPTYFAMARGAPGVPALELTKWFDTNYHYLVPEFDAPGPRLASAKPFLEFAEATSLLGRPAKPVLLGPIS
LLLLGKYHQRPLREHLEQLLPIYQEVLERLARAGAEWVQMDEPVLVQDRSDEELQLLRAAYRVLGEASARPKLMLQTYYG
SLDDASWDVVMSLPIEGIGLDFVRDDGNRALLNRHGFPQGKVIGAGVVNGRNIWRTNLVEALEKLEEIAEHVEPERIWVQ
PSCTLLHLPHDVRLETKLPAAVRALLAFAEQRLEELVILTRALNEGRAAILEELEQNAEAFRLALDEVRVYRRDVRERLA
ALTEDDFRRQSPYEMREPLQRARFHLPLFPTTTIGSFPQTPDVRQARARWRAGKLSDEDYWGFIRERIAEVIRRQEEIGL
DVLVHGEYERADMVEYFAEQLEGFVLTEHGWVQSYGSRVVRPPIIFGDVARPRPMTVEVSRCAQSLTEKPVKGMLTGPVT
ILNWSFVREDIPRREVAYQIALALRDEVRDLEAAGISMIQIDEPALREGLPLRRADWPEYLEWAVRAFRLASSGVRDDTQ
IHSHMCYSEFNDIIEAIDALDADVISIENSRSRGELLQAFQEYRYPRQIGPGVYDVHSARIPSVEEMLELLERALAFLRP
EQLWVNPDCGLKTRRPEEVWPSLSNMVEAARRLRARFTAQGQ

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=758, Percent_Identity=50.7915567282322, Blast_Score=725, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=780, Percent_Identity=46.5384615384615, Blast_Score=658, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 86985; Mature: 86854

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQSSVLGYPRIGKQRELKRALERYWAGEADAAALQETARAVREAAWRVQFQAGIDQIPC
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
NDFSLYDHVLDTICLVGLVPSRFGSAEPEVSLPTYFAMARGAPGVPALELTKWFDTNYHY
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHCCCCEE
LVPEFDAPGPRLASAKPFLEFAEATSLLGRPAKPVLLGPISLLLLGKYHQRPLREHLEQL
EECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
LPIYQEVLERLARAGAEWVQMDEPVLVQDRSDEELQLLRAAYRVLGEASARPKLMLQTYY
HHHHHHHHHHHHHCCCHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHC
GSLDDASWDVVMSLPIEGIGLDFVRDDGNRALLNRHGFPQGKVIGAGVVNGRNIWRTNLV
CCCCCCCCCEEEECCCCCCCCEEEECCCCEEHHHCCCCCCCCEEEEEECCCCHHHHHHHH
EALEKLEEIAEHVEPERIWVQPSCTLLHLPHDVRLETKLPAAVRALLAFAEQRLEELVIL
HHHHHHHHHHHHCCCCEEEECCCCEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHH
TRALNEGRAAILEELEQNAEAFRLALDEVRVYRRDVRERLAALTEDDFRRQSPYEMREPL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCHHHHHHH
QRARFHLPLFPTTTIGSFPQTPDVRQARARWRAGKLSDEDYWGFIRERIAEVIRRQEEIG
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
LDVLVHGEYERADMVEYFAEQLEGFVLTEHGWVQSYGSRVVRPPIIFGDVARPRPMTVEV
CEEEEECCCCHHHHHHHHHHHHCCEEEECCCHHHHHCCCCCCCCEEECCCCCCCCCEEEH
SRCAQSLTEKPVKGMLTGPVTILNWSFVREDIPRREVAYQIALALRDEVRDLEAAGISMI
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEE
QIDEPALREGLPLRRADWPEYLEWAVRAFRLASSGVRDDTQIHSHMCYSEFNDIIEAIDA
EECCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
LDADVISIENSRSRGELLQAFQEYRYPRQIGPGVYDVHSARIPSVEEMLELLERALAFLR
CCCCEEEECCCCCHHHHHHHHHHCCCCHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHC
PEQLWVNPDCGLKTRRPEEVWPSLSNMVEAARRLRARFTAQGQ
CCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AQSSVLGYPRIGKQRELKRALERYWAGEADAAALQETARAVREAAWRVQFQAGIDQIPC
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
NDFSLYDHVLDTICLVGLVPSRFGSAEPEVSLPTYFAMARGAPGVPALELTKWFDTNYHY
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHCCCCEE
LVPEFDAPGPRLASAKPFLEFAEATSLLGRPAKPVLLGPISLLLLGKYHQRPLREHLEQL
EECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
LPIYQEVLERLARAGAEWVQMDEPVLVQDRSDEELQLLRAAYRVLGEASARPKLMLQTYY
HHHHHHHHHHHHHCCCHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHC
GSLDDASWDVVMSLPIEGIGLDFVRDDGNRALLNRHGFPQGKVIGAGVVNGRNIWRTNLV
CCCCCCCCCEEEECCCCCCCCEEEECCCCEEHHHCCCCCCCCEEEEEECCCCHHHHHHHH
EALEKLEEIAEHVEPERIWVQPSCTLLHLPHDVRLETKLPAAVRALLAFAEQRLEELVIL
HHHHHHHHHHHHCCCCEEEECCCCEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHH
TRALNEGRAAILEELEQNAEAFRLALDEVRVYRRDVRERLAALTEDDFRRQSPYEMREPL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCHHHHHHH
QRARFHLPLFPTTTIGSFPQTPDVRQARARWRAGKLSDEDYWGFIRERIAEVIRRQEEIG
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
LDVLVHGEYERADMVEYFAEQLEGFVLTEHGWVQSYGSRVVRPPIIFGDVARPRPMTVEV
CEEEEECCCCHHHHHHHHHHHHCCEEEECCCHHHHHCCCCCCCCEEECCCCCCCCCEEEH
SRCAQSLTEKPVKGMLTGPVTILNWSFVREDIPRREVAYQIALALRDEVRDLEAAGISMI
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEE
QIDEPALREGLPLRRADWPEYLEWAVRAFRLASSGVRDDTQIHSHMCYSEFNDIIEAIDA
EECCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
LDADVISIENSRSRGELLQAFQEYRYPRQIGPGVYDVHSARIPSVEEMLELLERALAFLR
CCCCEEEECCCCCHHHHHHHHHHCCCCHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHC
PEQLWVNPDCGLKTRRPEEVWPSLSNMVEAARRLRARFTAQGQ
CCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA