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The map label for this gene is potD [H]

Identifier: 221632878

GI number: 221632878

Start: 858406

End: 859629

Strand: Reverse

Name: potD [H]

Synonym: trd_0878

Alternate gene names: 221632878

Gene position: 859629-858406 (Counterclockwise)

Preceding gene: 221632879

Following gene: 221632877

Centisome position: 42.92

GC content: 61.11

Gene sequence:

>1224_bases
GTGAGTCGAGATTGGGAGCGACTACTCAAGGACCTCGAGCGGGCCAGTCGCCTCCAACGGCGCCAGTTTCTCCGCGCCCT
GCTGGCGTCCGGGGTGACACTGGCGGGGGCGAGCGCACTGGCCGCTTGTCGCCAGCAGGCGACTCCGACGGCTGCTCCAG
CTGGGCAGACGCCGGCCGCAGCGCCGACGCAAGCGCAAGAGGGCGGCCTCCTCAAAGTCGCTGGATACGACAATCCTAAC
AAGTGGAAAGGGAAGACGCTCGTCTTCGCGGGGTTCGGCGGTGCGATGCAGGACGCCCAGCGCAAGGCGGTACTGGAGCC
GTTCCAGCGGCTCACCGGTGCGACCATCGTCGAAGATACCACCGACATTGGGAAATTGAAGGCGATGGTCGACGCCAAGG
CGGTCGAGTGGACGGTATCCCAGCAAGGAACCTTCGAAAGCCGTGTGCTGGGGCAGCAAGGGTACTTGGAACCGATCGAC
TACTCCATCGTCGATAAATCGAACTTTTTCGAGGATATCGCCGGAGAGTTCGACGTCGGAGTCATTTATTGGGCGCTTAT
CCTCGCCTATCGAACGGACAAATTCGGCAACAATCCGCCGCAGGGGTGGAAAGACTTCTGGGATGTGCAGAACTTCCCTG
GTCCGCGCGCCATGGATAAAGTCGACGGACCGGTTGGTAACCTCGAGTTCGCTCTGATGGCCGATGGGGTGCCGAAAGAG
CAAGTCTACCAGGTCATGCGCACAGCTGAGGGGATCGAGCGAGCCTTCAAGAAGATGGACGAGATCAAGCCACACGTGAC
GGTCTGGTGGGAAGCGGGTGCGCAGCCGGCCCAGCTGCTCACCGACGGCGAGGTCGATATGGCCAACGCCTGGAACGGGC
GGATCGATGCTGCTCAACGGCAAGGGGCGCCGATCGCGATCCAGTGGAACCAAGGGCTGCTCCGCACTGACTCCTTCATC
GTTCCCAAGGGGGTGAAGGAGAAGGAGTTGGCGATGGACTTCATCAACTTCGCGACGCGACCAGAAGTCCAGGCCGAGCT
GTCCAAGTACATTCCCTACAGCCCAACGAACGCGAAGGCCTTCGACCTGCTTCCCGAAGACCTCAAGGCCCGCTTGCCGA
GTGCGCCCGCACAAAAAGAGAAGATGCTCTTTGCCGACGCTGAATTCTGGCTGGCTAACCTCGACAAGCTCACAGAGCGC
TTCAACGACTGGCTGACGAAGTGA

Upstream 100 bases:

>100_bases
GGTGGATTCCCACGCCGAACCAAGCTGCTTTCAGCGAACTCGAGGACTGATCGTGGCATACTGTGTGCGGGTCGTTGACG
CGTGAGGAAGGAGGATTGCG

Downstream 100 bases:

>100_bases
CGCGCAGTCCAGTGACGCTGCTGCCGGGGGAGTGCTCGCTCCCCCGGTAGCGTGTCATCGCATCGATGTGTACAGTAGTG
TCGGGGAAGCTCCGGTTGAG

Product: polyamine ABC transporter periplasmic polyamine-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MSRDWERLLKDLERASRLQRRQFLRALLASGVTLAGASALAACRQQATPTAAPAGQTPAAAPTQAQEGGLLKVAGYDNPN
KWKGKTLVFAGFGGAMQDAQRKAVLEPFQRLTGATIVEDTTDIGKLKAMVDAKAVEWTVSQQGTFESRVLGQQGYLEPID
YSIVDKSNFFEDIAGEFDVGVIYWALILAYRTDKFGNNPPQGWKDFWDVQNFPGPRAMDKVDGPVGNLEFALMADGVPKE
QVYQVMRTAEGIERAFKKMDEIKPHVTVWWEAGAQPAQLLTDGEVDMANAWNGRIDAAQRQGAPIAIQWNQGLLRTDSFI
VPKGVKEKELAMDFINFATRPEVQAELSKYIPYSPTNAKAFDLLPEDLKARLPSAPAQKEKMLFADAEFWLANLDKLTER
FNDWLTK

Sequences:

>Translated_407_residues
MSRDWERLLKDLERASRLQRRQFLRALLASGVTLAGASALAACRQQATPTAAPAGQTPAAAPTQAQEGGLLKVAGYDNPN
KWKGKTLVFAGFGGAMQDAQRKAVLEPFQRLTGATIVEDTTDIGKLKAMVDAKAVEWTVSQQGTFESRVLGQQGYLEPID
YSIVDKSNFFEDIAGEFDVGVIYWALILAYRTDKFGNNPPQGWKDFWDVQNFPGPRAMDKVDGPVGNLEFALMADGVPKE
QVYQVMRTAEGIERAFKKMDEIKPHVTVWWEAGAQPAQLLTDGEVDMANAWNGRIDAAQRQGAPIAIQWNQGLLRTDSFI
VPKGVKEKELAMDFINFATRPEVQAELSKYIPYSPTNAKAFDLLPEDLKARLPSAPAQKEKMLFADAEFWLANLDKLTER
FNDWLTK
>Mature_406_residues
SRDWERLLKDLERASRLQRRQFLRALLASGVTLAGASALAACRQQATPTAAPAGQTPAAAPTQAQEGGLLKVAGYDNPNK
WKGKTLVFAGFGGAMQDAQRKAVLEPFQRLTGATIVEDTTDIGKLKAMVDAKAVEWTVSQQGTFESRVLGQQGYLEPIDY
SIVDKSNFFEDIAGEFDVGVIYWALILAYRTDKFGNNPPQGWKDFWDVQNFPGPRAMDKVDGPVGNLEFALMADGVPKEQ
VYQVMRTAEGIERAFKKMDEIKPHVTVWWEAGAQPAQLLTDGEVDMANAWNGRIDAAQRQGAPIAIQWNQGLLRTDSFIV
PKGVKEKELAMDFINFATRPEVQAELSKYIPYSPTNAKAFDLLPEDLKARLPSAPAQKEKMLFADAEFWLANLDKLTERF
NDWLTK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=307, Percent_Identity=25.0814332247557, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 45205; Mature: 45074

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRDWERLLKDLERASRLQRRQFLRALLASGVTLAGASALAACRQQATPTAAPAGQTPAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
APTQAQEGGLLKVAGYDNPNKWKGKTLVFAGFGGAMQDAQRKAVLEPFQRLTGATIVEDT
CCCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEECCC
TDIGKLKAMVDAKAVEWTVSQQGTFESRVLGQQGYLEPIDYSIVDKSNFFEDIAGEFDVG
HHHHHHHHHHHHHHHEEECCCCCCHHHHHHCCCCCCCCCCEEEECCHHHHHHHCCCCCHH
VIYWALILAYRTDKFGNNPPQGWKDFWDVQNFPGPRAMDKVDGPVGNLEFALMADGVPKE
HHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCHHHCCCCCCCEEEEEEECCCCHH
QVYQVMRTAEGIERAFKKMDEIKPHVTVWWEAGAQPAQLLTDGEVDMANAWNGRIDAAQR
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCCCEECCCCCCCCCHHHH
QGAPIAIQWNQGLLRTDSFIVPKGVKEKELAMDFINFATRPEVQAELSKYIPYSPTNAKA
CCCCEEEEECCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCH
FDLLPEDLKARLPSAPAQKEKMLFADAEFWLANLDKLTERFNDWLTK
HHCCCHHHHHHCCCCCCCHHHEEEECHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRDWERLLKDLERASRLQRRQFLRALLASGVTLAGASALAACRQQATPTAAPAGQTPAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
APTQAQEGGLLKVAGYDNPNKWKGKTLVFAGFGGAMQDAQRKAVLEPFQRLTGATIVEDT
CCCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEECCC
TDIGKLKAMVDAKAVEWTVSQQGTFESRVLGQQGYLEPIDYSIVDKSNFFEDIAGEFDVG
HHHHHHHHHHHHHHHEEECCCCCCHHHHHHCCCCCCCCCCEEEECCHHHHHHHCCCCCHH
VIYWALILAYRTDKFGNNPPQGWKDFWDVQNFPGPRAMDKVDGPVGNLEFALMADGVPKE
HHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCHHHCCCCCCCEEEEEEECCCCHH
QVYQVMRTAEGIERAFKKMDEIKPHVTVWWEAGAQPAQLLTDGEVDMANAWNGRIDAAQR
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCCCEECCCCCCCCCHHHH
QGAPIAIQWNQGLLRTDSFIVPKGVKEKELAMDFINFATRPEVQAELSKYIPYSPTNAKA
CCCCEEEEECCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCH
FDLLPEDLKARLPSAPAQKEKMLFADAEFWLANLDKLTERFNDWLTK
HHCCCHHHHHHCCCCCCCHHHEEEECHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]