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The map label for this gene is potB [H]

Identifier: 221632877

GI number: 221632877

Start: 857383

End: 858285

Strand: Reverse

Name: potB [H]

Synonym: trd_0876

Alternate gene names: 221632877

Gene position: 858285-857383 (Counterclockwise)

Preceding gene: 221632878

Following gene: 221632876

Centisome position: 42.85

GC content: 56.7

Gene sequence:

>903_bases
ATGACGACAGGTGAGGCGCTTGCCAGTGCGGCGTATCAGGGAAGCGCGCTGAGTCGTCGGTTGGAGCTGCGCCGGCAAGT
CGGATATCTGGTGCTCTTGCTCCCGGCGTTGATCGTGCTTCTCGTGCTTTTCGTGTATCCGATCCTCCGACTTCTCGTCA
CGAGCGTCTACGACGGAGGTCAACTGACGCTCGAACATTATCGACGGATCGTCGAGGTCGACCTTTATCTTCGGGTCTTA
CGTACGACCTTCACGATCGCACTACAGGTAACGCTCCTCTGCCTGGCACTGGGTTACCCGTTAGCGTATTTTCTGGCGAC
ACTCCGCGCCCGGACAGCACGGCTACTCATGATCTTGGTGCTGATTCCGTTTTGGACGAGCATCCTGGTGCGCACGTACG
CGTGGATGGTATTACTCCAACGCCAGGGTGTCTTCAATCGCTGGCTCTTGGAACTCGGGTTGATCGATGAACCGCTTCGT
ATGATGTATAACCGCATCGGCGTACTCGTCGGTATGACGCATGTGCTCTTGCCGTTCATGGTGCTGCCGACCTATGCGGT
CATGCGGGGAATCGATCGCAATCTCCTGCGAGCAGCGGCGAATCTGGGAGCCTCGCCGCTCCAGTCGTTCCTGCGCGTGT
TCCTTCCATTGAGTCTCCCCGGGGTCGCGGCTGGTAGTTTGCTCGTCTTCATTCTGGCGCTCGGTTTCTTCATCACTCCA
GCTTTGATGGGTGGTCGAAACGATATGATGATCGCCCAGCTCATCGAGACGCAGATTCGGACACAGCTGAACTTCGCGTT
CGCAGCAGCTCTCGCGACTGTTCTTCTCGTGGTGACGCTGATCATCTTTGCCATTTACAATCGGTTACTCGGACTCGATA
AAATGTTCGGGGGTGGCGTCTGA

Upstream 100 bases:

>100_bases
CTGCCGGGGGAGTGCTCGCTCCCCCGGTAGCGTGTCATCGCATCGATGTGTACAGTAGTGTCGGGGAAGCTCCGGTTGAG
TGGTTCGAGGGGCGTCGAGG

Downstream 100 bases:

>100_bases
TGGCCGAAGCAGCGACTCCGCTCGAACGGCTCGAGATAGCAGTACAGCCAAGGCGAAGCCGGCGCGCGATACCGTGGGCG
CGTATTGTCCTGGGGATATT

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MTTGEALASAAYQGSALSRRLELRRQVGYLVLLLPALIVLLVLFVYPILRLLVTSVYDGGQLTLEHYRRIVEVDLYLRVL
RTTFTIALQVTLLCLALGYPLAYFLATLRARTARLLMILVLIPFWTSILVRTYAWMVLLQRQGVFNRWLLELGLIDEPLR
MMYNRIGVLVGMTHVLLPFMVLPTYAVMRGIDRNLLRAAANLGASPLQSFLRVFLPLSLPGVAAGSLLVFILALGFFITP
ALMGGRNDMMIAQLIETQIRTQLNFAFAAALATVLLVVTLIIFAIYNRLLGLDKMFGGGV

Sequences:

>Translated_300_residues
MTTGEALASAAYQGSALSRRLELRRQVGYLVLLLPALIVLLVLFVYPILRLLVTSVYDGGQLTLEHYRRIVEVDLYLRVL
RTTFTIALQVTLLCLALGYPLAYFLATLRARTARLLMILVLIPFWTSILVRTYAWMVLLQRQGVFNRWLLELGLIDEPLR
MMYNRIGVLVGMTHVLLPFMVLPTYAVMRGIDRNLLRAAANLGASPLQSFLRVFLPLSLPGVAAGSLLVFILALGFFITP
ALMGGRNDMMIAQLIETQIRTQLNFAFAAALATVLLVVTLIIFAIYNRLLGLDKMFGGGV
>Mature_299_residues
TTGEALASAAYQGSALSRRLELRRQVGYLVLLLPALIVLLVLFVYPILRLLVTSVYDGGQLTLEHYRRIVEVDLYLRVLR
TTFTIALQVTLLCLALGYPLAYFLATLRARTARLLMILVLIPFWTSILVRTYAWMVLLQRQGVFNRWLLELGLIDEPLRM
MYNRIGVLVGMTHVLLPFMVLPTYAVMRGIDRNLLRAAANLGASPLQSFLRVFLPLSLPGVAAGSLLVFILALGFFITPA
LMGGRNDMMIAQLIETQIRTQLNFAFAAALATVLLVVTLIIFAIYNRLLGLDKMFGGGV

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=256, Percent_Identity=40.625, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1787080, Length=227, Percent_Identity=37.0044052863436, Blast_Score=171, Evalue=8e-44,
Organism=Escherichia coli, GI1787713, Length=249, Percent_Identity=34.136546184739, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787368, Length=132, Percent_Identity=31.8181818181818, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33460; Mature: 33329

Theoretical pI: Translated: 10.63; Mature: 10.63

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTGEALASAAYQGSALSRRLELRRQVGYLVLLLPALIVLLVLFVYPILRLLVTSVYDGG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QLTLEHYRRIVEVDLYLRVLRTTFTIALQVTLLCLALGYPLAYFLATLRARTARLLMILV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIPFWTSILVRTYAWMVLLQRQGVFNRWLLELGLIDEPLRMMYNRIGVLVGMTHVLLPFM
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VLPTYAVMRGIDRNLLRAAANLGASPLQSFLRVFLPLSLPGVAAGSLLVFILALGFFITP
HHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
ALMGGRNDMMIAQLIETQIRTQLNFAFAAALATVLLVVTLIIFAIYNRLLGLDKMFGGGV
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TTGEALASAAYQGSALSRRLELRRQVGYLVLLLPALIVLLVLFVYPILRLLVTSVYDGG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC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PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]