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The map label for this gene is rpoD [H]

Identifier: 221632805

GI number: 221632805

Start: 781796

End: 782905

Strand: Reverse

Name: rpoD [H]

Synonym: trd_0799

Alternate gene names: 221632805

Gene position: 782905-781796 (Counterclockwise)

Preceding gene: 221632809

Following gene: 221632801

Centisome position: 39.09

GC content: 58.29

Gene sequence:

>1110_bases
ATGGATCTGCATGCCTTGGATGGAACGAGCGATTCCTACTTCCTCGCCGACGACGTGTTTCCCTCACTGACCGATTTGGA
TGAGCTTTCCGCCGACGAAACCTTGAGGACAAACGAGAGCGAAGGCCTCCTTCCCTCACGACACTCACGCCTCCAGCGAC
GATTGCGCGATCACCCCGAACGCTTCGATGACGACGGCCTCAGTACCAGCGATACCGTTCGTCTCTATCTTCAAGAAATC
GGTGAAACAGCGCTTCTCACCCCCCAAGAGGAAGTCTGGCTCGCTCAGCGAATCGAACGAGGCAAGATCGCCAAAGTTCG
GCTCGAGCGGGGAGACTACAAGTCGGAAGACGAACGACTGGCCCTCGTCGCTGACAAGGAAGATGGTGAACGTGCCCGCG
CTCATTTGATTCAAGCCAACCTGCGGCTGGTAGTCAGCGTCGCCAAGAAATATGTCGGACGTGGGCTCTCTTTCCTCGAC
CTCATTCAAGAGGGCAATATCGGCCTCATGAAGGCGACTGAGAAATTCGACTACAAGCGGGGCTACAAGTTCTCCACCTA
TGCGACGTGGTGGATCCGGCAAGCAATCACGCGGGCGATTTCCGATCAGAGTCGGACCATCCGCTTGCCCGTCCATGTCG
GCGAGACCATCAACCGCATCAAGAAGATCAGCCACCGCCTCCAACAGATTCTCGAACGTGAACCGACCCAATTGGAGGTT
GCTCGTGCCCTCGATATGCCGGAAGAAAAGATTCGCCAGATCCTCGAAGCAGCACGCCTCCCCGTCTCGCTGGAAGCACC
GATCGGTCAAGATGGTGACGCGTTCTTGGGCGACTTCATCGAGGACGAGACACAACCAGCCCCCCTAGAAATCGCTTCCC
AGCAACTCCTTCGGCAACAGCTCGAGGAAGCGTTGAGTCGCCTCACTGAGCGCGAACGGCGCATCATCATCCTGCGATAC
GGCCTCGAAGACGGACAGTTCCGCACCCTGGAGGAAGTCGGGCGCGAGTTCGGTATCACCCGCGAGCGGATCCGGCAGAT
CGAGGCGAAGGCCCTGCGTAAGCTGCGCCACCCGAGCTGCAGCCGCTTCTTGCGCGGCTATCTAGACTGA

Upstream 100 bases:

>100_bases
ACGACCGCTCGCCGGAAGATGGCCCACGACGTCATGATCTCATGGTTCGACAACGTCGATGCGGTGCCGACTGGTGAGTG
GGGGTTGAAACATGGACTCC

Downstream 100 bases:

>100_bases
TCATAACCCTTTTCTGCTATTCTCCCTTCCGTAGGAAACCAACGGAAGGGAGAATATCATGCTGACGATCGAGTTTTGGT
CCGATATCCAATGCCCTTGG

Product: sigma factor MysA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MDLHALDGTSDSYFLADDVFPSLTDLDELSADETLRTNESEGLLPSRHSRLQRRLRDHPERFDDDGLSTSDTVRLYLQEI
GETALLTPQEEVWLAQRIERGKIAKVRLERGDYKSEDERLALVADKEDGERARAHLIQANLRLVVSVAKKYVGRGLSFLD
LIQEGNIGLMKATEKFDYKRGYKFSTYATWWIRQAITRAISDQSRTIRLPVHVGETINRIKKISHRLQQILEREPTQLEV
ARALDMPEEKIRQILEAARLPVSLEAPIGQDGDAFLGDFIEDETQPAPLEIASQQLLRQQLEEALSRLTERERRIIILRY
GLEDGQFRTLEEVGREFGITRERIRQIEAKALRKLRHPSCSRFLRGYLD

Sequences:

>Translated_369_residues
MDLHALDGTSDSYFLADDVFPSLTDLDELSADETLRTNESEGLLPSRHSRLQRRLRDHPERFDDDGLSTSDTVRLYLQEI
GETALLTPQEEVWLAQRIERGKIAKVRLERGDYKSEDERLALVADKEDGERARAHLIQANLRLVVSVAKKYVGRGLSFLD
LIQEGNIGLMKATEKFDYKRGYKFSTYATWWIRQAITRAISDQSRTIRLPVHVGETINRIKKISHRLQQILEREPTQLEV
ARALDMPEEKIRQILEAARLPVSLEAPIGQDGDAFLGDFIEDETQPAPLEIASQQLLRQQLEEALSRLTERERRIIILRY
GLEDGQFRTLEEVGREFGITRERIRQIEAKALRKLRHPSCSRFLRGYLD
>Mature_369_residues
MDLHALDGTSDSYFLADDVFPSLTDLDELSADETLRTNESEGLLPSRHSRLQRRLRDHPERFDDDGLSTSDTVRLYLQEI
GETALLTPQEEVWLAQRIERGKIAKVRLERGDYKSEDERLALVADKEDGERARAHLIQANLRLVVSVAKKYVGRGLSFLD
LIQEGNIGLMKATEKFDYKRGYKFSTYATWWIRQAITRAISDQSRTIRLPVHVGETINRIKKISHRLQQILEREPTQLEV
ARALDMPEEKIRQILEAARLPVSLEAPIGQDGDAFLGDFIEDETQPAPLEIASQQLLRQQLEEALSRLTERERRIIILRY
GLEDGQFRTLEEVGREFGITRERIRQIEAKALRKLRHPSCSRFLRGYLD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This may be the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=239, Percent_Identity=60.2510460251046, Blast_Score=304, Evalue=5e-84,
Organism=Escherichia coli, GI1789098, Length=286, Percent_Identity=40.5594405594406, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1789871, Length=238, Percent_Identity=30.672268907563, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788231, Length=201, Percent_Identity=29.8507462686567, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 42587; Mature: 42587

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLHALDGTSDSYFLADDVFPSLTDLDELSADETLRTNESEGLLPSRHSRLQRRLRDHPE
CCCCCCCCCCCCEEEEHHCCCCCCCHHHCCCCCHHCCCCCCCCCCHHHHHHHHHHHHCCC
RFDDDGLSTSDTVRLYLQEIGETALLTPQEEVWLAQRIERGKIAKVRLERGDYKSEDERL
CCCCCCCCCHHHHHHHHHHHCCCEECCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEE
ALVADKEDGERARAHLIQANLRLVVSVAKKYVGRGLSFLDLIQEGNIGLMKATEKFDYKR
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEECHHHHHHHC
GYKFSTYATWWIRQAITRAISDQSRTIRLPVHVGETINRIKKISHRLQQILEREPTQLEV
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ARALDMPEEKIRQILEAARLPVSLEAPIGQDGDAFLGDFIEDETQPAPLEIASQQLLRQQ
HHHHCCCHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHH
LEEALSRLTERERRIIILRYGLEDGQFRTLEEVGREFGITRERIRQIEAKALRKLRHPSC
HHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCH
SRFLRGYLD
HHHHHHCCC
>Mature Secondary Structure
MDLHALDGTSDSYFLADDVFPSLTDLDELSADETLRTNESEGLLPSRHSRLQRRLRDHPE
CCCCCCCCCCCCEEEEHHCCCCCCCHHHCCCCCHHCCCCCCCCCCHHHHHHHHHHHHCCC
RFDDDGLSTSDTVRLYLQEIGETALLTPQEEVWLAQRIERGKIAKVRLERGDYKSEDERL
CCCCCCCCCHHHHHHHHHHHCCCEECCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEE
ALVADKEDGERARAHLIQANLRLVVSVAKKYVGRGLSFLDLIQEGNIGLMKATEKFDYKR
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEECHHHHHHHC
GYKFSTYATWWIRQAITRAISDQSRTIRLPVHVGETINRIKKISHRLQQILEREPTQLEV
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ARALDMPEEKIRQILEAARLPVSLEAPIGQDGDAFLGDFIEDETQPAPLEIASQQLLRQQ
HHHHCCCHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHH
LEEALSRLTERERRIIILRYGLEDGQFRTLEEVGREFGITRERIRQIEAKALRKLRHPSC
HHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCH
SRFLRGYLD
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1923759 [H]