Click here to switch to the map view.

The map label for this gene is ruvB

Identifier: 221632730

GI number: 221632730

Start: 712711

End: 713772

Strand: Reverse

Name: ruvB

Synonym: trd_0718

Alternate gene names: 221632730

Gene position: 713772-712711 (Counterclockwise)

Preceding gene: 221632731

Following gene: 221632729

Centisome position: 35.64

GC content: 61.96

Gene sequence:

>1062_bases
ATGAACGAGCCACGCATCGTCGCACCACAACCGACCACGAGCGAGCAGTATTTCGAGGAGACCTTGCGCCCGCGTCGCCT
TGCCGAGTACATCGGGCAGGATCGTGTCAAGGAAAGCCTCGCCATCGCCATCAGAGCCGCCCAGGAGCGTGGTGAGCCAC
TCGATCATCTCCTCTTCTATGGCCCTCCAGGACTCGGTAAAACGACGCTCGCCGGGATCATCGCCGCGGAGATGGGGGTT
AACCTCCGGATCACCAGCGGTCCAGCCATCGAGCGTCCGGGCGATCTCGTTTCGATCCTGACCAATCTTCGTCCTGGAGA
CGTCCTCTTCATCGACGAAATCCATCGCCTTAACCGGCTGGTCGAAGAAATCCTCTACCCCGCGATGGAAGACTTTGCGG
TCGATCTGGTGATCGGTAAAGGCCCAGCAGCACGCAGTGTGCGCATTGCGCTCCCGCGCTTCACGTTGGTCGGGGCAACC
ACGCGGCTCGCACTGCTCACCTCCCCGCTGCGCGATCGCTTCGGCGCGACCTATCGACTGGACTTTTACGACACCGCGGC
GCTGCGAGCCATCGTCGAGCGTGCCGCCCGCATCCTCCAGGTGCCTATTACCTCCGATGGCGCGGAGGAGATCGCCCGGC
GTGGTCGCGGTACACCTCGTATAGCGATCCGTCTCCTCAAGCGTGTGCGCGACTACGCCCAGGTCATCGGCGACGGCACG
ATCACCCGAGAACTCGCTCGCCTGGCCCTCGATCAGCTCGCCGTCGACGAACTCGGCCTCGATGAAGTCGACCGGCTCAT
CCTCCGGACCCTGGTGGAGAAATTCGACGGTGGTCCAGTCGGTATCCAGACATTAGCCGCCGCGACAAGCGAAGAACCGG
ATACGATCGAAAGTGTCTACGAGCCATATCTCCTCCAGCTCGGGTTCCTTCAGCGCACACCGCGCGGACGGATCGCCACC
CGTCGAGCCTACGAGCATCTCGGACTCACTTATCCAGAAAACCGGTTGCAGCTCGAAATACCGTTCGAGCATGCCGCGTC
GGAAAGATCCAGCGATGCTTGA

Upstream 100 bases:

>100_bases
CATCCTTCTCGTCGAGGAAGCGGAGTTGCCAGAGAAACCTGGCACTTGAGACGAACTCCTGTTCTTCTCTCTGGTATCCT
CATACGTGTAGGGATGAGCG

Downstream 100 bases:

>100_bases
GGAAGCGGCACTCTCTCTTCATGAATACGATTACGAGCTTCCGGAGGAATTGATCGCACAGGAGCCGATCGAGCCGCGCG
ATGCTGCCCGGCTGATGGTT

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MNEPRIVAPQPTTSEQYFEETLRPRRLAEYIGQDRVKESLAIAIRAAQERGEPLDHLLFYGPPGLGKTTLAGIIAAEMGV
NLRITSGPAIERPGDLVSILTNLRPGDVLFIDEIHRLNRLVEEILYPAMEDFAVDLVIGKGPAARSVRIALPRFTLVGAT
TRLALLTSPLRDRFGATYRLDFYDTAALRAIVERAARILQVPITSDGAEEIARRGRGTPRIAIRLLKRVRDYAQVIGDGT
ITRELARLALDQLAVDELGLDEVDRLILRTLVEKFDGGPVGIQTLAAATSEEPDTIESVYEPYLLQLGFLQRTPRGRIAT
RRAYEHLGLTYPENRLQLEIPFEHAASERSSDA

Sequences:

>Translated_353_residues
MNEPRIVAPQPTTSEQYFEETLRPRRLAEYIGQDRVKESLAIAIRAAQERGEPLDHLLFYGPPGLGKTTLAGIIAAEMGV
NLRITSGPAIERPGDLVSILTNLRPGDVLFIDEIHRLNRLVEEILYPAMEDFAVDLVIGKGPAARSVRIALPRFTLVGAT
TRLALLTSPLRDRFGATYRLDFYDTAALRAIVERAARILQVPITSDGAEEIARRGRGTPRIAIRLLKRVRDYAQVIGDGT
ITRELARLALDQLAVDELGLDEVDRLILRTLVEKFDGGPVGIQTLAAATSEEPDTIESVYEPYLLQLGFLQRTPRGRIAT
RRAYEHLGLTYPENRLQLEIPFEHAASERSSDA
>Mature_353_residues
MNEPRIVAPQPTTSEQYFEETLRPRRLAEYIGQDRVKESLAIAIRAAQERGEPLDHLLFYGPPGLGKTTLAGIIAAEMGV
NLRITSGPAIERPGDLVSILTNLRPGDVLFIDEIHRLNRLVEEILYPAMEDFAVDLVIGKGPAARSVRIALPRFTLVGAT
TRLALLTSPLRDRFGATYRLDFYDTAALRAIVERAARILQVPITSDGAEEIARRGRGTPRIAIRLLKRVRDYAQVIGDGT
ITRELARLALDQLAVDELGLDEVDRLILRTLVEKFDGGPVGIQTLAAATSEEPDTIESVYEPYLLQLGFLQRTPRGRIAT
RRAYEHLGLTYPENRLQLEIPFEHAASERSSDA

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=329, Percent_Identity=59.5744680851064, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1787119, Length=130, Percent_Identity=33.8461538461538, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_THERP (B9KZ09)

Other databases:

- EMBL:   CP001275
- RefSeq:   YP_002521951.1
- GeneID:   7355946
- GenomeReviews:   CP001275_GR
- KEGG:   tro:trd_0718
- OMA:   EKFDGGP
- ProtClustDB:   CLSK2807652
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 39208; Mature: 39208

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEPRIVAPQPTTSEQYFEETLRPRRLAEYIGQDRVKESLAIAIRAAQERGEPLDHLLFY
CCCCCEECCCCCCHHHHHHHHHCHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHEEEE
GPPGLGKTTLAGIIAAEMGVNLRITSGPAIERPGDLVSILTNLRPGDVLFIDEIHRLNRL
CCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHCCCCCCEEEHHHHHHHHHH
VEEILYPAMEDFAVDLVIGKGPAARSVRIALPRFTLVGATTRLALLTSPLRDRFGATYRL
HHHHHHHHHHHHEEEEEECCCCCCCEEEEECCCEEEHHHHHHHHHHHHHHHHHCCCEEEE
DFYDTAALRAIVERAARILQVPITSDGAEEIARRGRGTPRIAIRLLKRVRDYAQVIGDGT
EHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCH
ITRELARLALDQLAVDELGLDEVDRLILRTLVEKFDGGPVGIQTLAAATSEEPDTIESVY
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHH
EPYLLQLGFLQRTPRGRIATRRAYEHLGLTYPENRLQLEIPFEHAASERSSDA
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEEECHHHHHCCCCCCC
>Mature Secondary Structure
MNEPRIVAPQPTTSEQYFEETLRPRRLAEYIGQDRVKESLAIAIRAAQERGEPLDHLLFY
CCCCCEECCCCCCHHHHHHHHHCHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHEEEE
GPPGLGKTTLAGIIAAEMGVNLRITSGPAIERPGDLVSILTNLRPGDVLFIDEIHRLNRL
CCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHCCCCCCEEEHHHHHHHHHH
VEEILYPAMEDFAVDLVIGKGPAARSVRIALPRFTLVGATTRLALLTSPLRDRFGATYRL
HHHHHHHHHHHHEEEEEECCCCCCCEEEEECCCEEEHHHHHHHHHHHHHHHHHCCCEEEE
DFYDTAALRAIVERAARILQVPITSDGAEEIARRGRGTPRIAIRLLKRVRDYAQVIGDGT
EHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCH
ITRELARLALDQLAVDELGLDEVDRLILRTLVEKFDGGPVGIQTLAAATSEEPDTIESVY
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHH
EPYLLQLGFLQRTPRGRIATRRAYEHLGLTYPENRLQLEIPFEHAASERSSDA
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEEECHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA