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The map label for this gene is queA [H]

Identifier: 221632729

GI number: 221632729

Start: 711657

End: 712718

Strand: Reverse

Name: queA [H]

Synonym: trd_0716

Alternate gene names: 221632729

Gene position: 712718-711657 (Counterclockwise)

Preceding gene: 221632730

Following gene: 221632726

Centisome position: 35.58

GC content: 62.34

Gene sequence:

>1062_bases
ATGCTTGAGGAAGCGGCACTCTCTCTTCATGAATACGATTACGAGCTTCCGGAGGAATTGATCGCACAGGAGCCGATCGA
GCCGCGCGATGCTGCCCGGCTGATGGTTGTTCGCCGAGCGACACACTCTATCGAGCATCGCATCTTTCGTGAACTTCCCT
CCCTCCTCGATCCTGGAGACCTTTTGATCGTCAACGACTCGCGGGTCCTCCCAGCCCGTCTCTTGGGTCACCGCCTGACC
GGAGGGAAAGTCGAGATCCTCTTGGTCCAACCGCTCGAAGGAACCACCGTTTGGCTCGCGCTCGCCCGCCCAGCTCGGAA
GCTGCGCCCCGGAGAGACGATCACGATCCAACCTCGCGATCCACAGTTCGCTCAGCCAGCGCCCCTGCGCATCCGCGAGC
GCCGTTCGGGCGGCCAGGTCATCGTCGAGCTGGATCCCGGTATCGCCGAACGCTTGGACGCTTTCGGGCACGTGCCGCTC
CCGCCGTATATCCACCGTCCGCTGTCCGACCCGGAACGCTACCAAACGGTTTATGCAAGGCATCCCGGCTCGGTAGCCGC
GCCGACTGCTGGGCTCCATTTCACGGAACGACTGCTGGACGAGTTACGCCAACGGGGAATCCGCATCGCGCCGCTCACCC
TGCATGTCGGAATCGGCACCTTCAAGCCGATCGAGGTGACCGACGTCCGCTTGCACAAGATGCATGCGGAGTGGTACGCG
GTGCCAGCCGAGACAGTCTCGCTGATTCGAGAAACTCGTGCTGCGAACCGCCGGATCGTTGCCGTGGGCACGACGGCTGC
CCGCACCCTCGAATCGATCGCCGATGCTCTGACAGGAGACACCGAGGGTCCTATCACTGGCTGGACTGACCTTTACATCT
ATCCTGGCTACCACTGGCGCGTGGTCGACGCGCTGATCACCAACTTCCACTTGCCCCGCAGCACCCTGATCCTGCTCGTT
GCGAGCTTCGCCGGGCGCGAACTGATCCTGGAGGCGTATCGAGAGGCCGTCCAGCAACGCTATCGCTTCTACAGTTTCGG
CGACGCGATGCTCATTCTCTGA

Upstream 100 bases:

>100_bases
GCCACCCGTCGAGCCTACGAGCATCTCGGACTCACTTATCCAGAAAACCGGTTGCAGCTCGAAATACCGTTCGAGCATGC
CGCGTCGGAAAGATCCAGCG

Downstream 100 bases:

>100_bases
CCCCCGCACTGCCAACAGCGAGCTGCTCGAGTGCGCTCGCCAACTTTTCGAGATCCTCGAACGAGGTGAAGTAGGCGCAG
GATACGCGTATGCCGTTCGG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MLEEAALSLHEYDYELPEELIAQEPIEPRDAARLMVVRRATHSIEHRIFRELPSLLDPGDLLIVNDSRVLPARLLGHRLT
GGKVEILLVQPLEGTTVWLALARPARKLRPGETITIQPRDPQFAQPAPLRIRERRSGGQVIVELDPGIAERLDAFGHVPL
PPYIHRPLSDPERYQTVYARHPGSVAAPTAGLHFTERLLDELRQRGIRIAPLTLHVGIGTFKPIEVTDVRLHKMHAEWYA
VPAETVSLIRETRAANRRIVAVGTTAARTLESIADALTGDTEGPITGWTDLYIYPGYHWRVVDALITNFHLPRSTLILLV
ASFAGRELILEAYREAVQQRYRFYSFGDAMLIL

Sequences:

>Translated_353_residues
MLEEAALSLHEYDYELPEELIAQEPIEPRDAARLMVVRRATHSIEHRIFRELPSLLDPGDLLIVNDSRVLPARLLGHRLT
GGKVEILLVQPLEGTTVWLALARPARKLRPGETITIQPRDPQFAQPAPLRIRERRSGGQVIVELDPGIAERLDAFGHVPL
PPYIHRPLSDPERYQTVYARHPGSVAAPTAGLHFTERLLDELRQRGIRIAPLTLHVGIGTFKPIEVTDVRLHKMHAEWYA
VPAETVSLIRETRAANRRIVAVGTTAARTLESIADALTGDTEGPITGWTDLYIYPGYHWRVVDALITNFHLPRSTLILLV
ASFAGRELILEAYREAVQQRYRFYSFGDAMLIL
>Mature_353_residues
MLEEAALSLHEYDYELPEELIAQEPIEPRDAARLMVVRRATHSIEHRIFRELPSLLDPGDLLIVNDSRVLPARLLGHRLT
GGKVEILLVQPLEGTTVWLALARPARKLRPGETITIQPRDPQFAQPAPLRIRERRSGGQVIVELDPGIAERLDAFGHVPL
PPYIHRPLSDPERYQTVYARHPGSVAAPTAGLHFTERLLDELRQRGIRIAPLTLHVGIGTFKPIEVTDVRLHKMHAEWYA
VPAETVSLIRETRAANRRIVAVGTTAARTLESIADALTGDTEGPITGWTDLYIYPGYHWRVVDALITNFHLPRSTLILLV
ASFAGRELILEAYREAVQQRYRFYSFGDAMLIL

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=346, Percent_Identity=43.9306358381503, Blast_Score=299, Evalue=2e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39775; Mature: 39775

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEEAALSLHEYDYELPEELIAQEPIEPRDAARLMVVRRATHSIEHRIFRELPSLLDPGD
CCCHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LLIVNDSRVLPARLLGHRLTGGKVEILLVQPLEGTTVWLALARPARKLRPGETITIQPRD
EEEECCCCCCHHHHHCCCCCCCEEEEEEEECCCCCEEEEEECCCHHHCCCCCEEEECCCC
PQFAQPAPLRIRERRSGGQVIVELDPGIAERLDAFGHVPLPPYIHRPLSDPERYQTVYAR
CCCCCCCCCEEEECCCCCEEEEEECCCHHHHHHHCCCCCCCHHHCCCCCCHHHHHEEEEE
HPGSVAAPTAGLHFTERLLDELRQRGIRIAPLTLHVGIGTFKPIEVTDVRLHKMHAEWYA
CCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEEEHHHHEEECHHHEE
VPAETVSLIRETRAANRRIVAVGTTAARTLESIADALTGDTEGPITGWTDLYIYPGYHWR
CCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHH
VVDALITNFHLPRSTLILLVASFAGRELILEAYREAVQQRYRFYSFGDAMLIL
HHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure
MLEEAALSLHEYDYELPEELIAQEPIEPRDAARLMVVRRATHSIEHRIFRELPSLLDPGD
CCCHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LLIVNDSRVLPARLLGHRLTGGKVEILLVQPLEGTTVWLALARPARKLRPGETITIQPRD
EEEECCCCCCHHHHHCCCCCCCEEEEEEEECCCCCEEEEEECCCHHHCCCCCEEEECCCC
PQFAQPAPLRIRERRSGGQVIVELDPGIAERLDAFGHVPLPPYIHRPLSDPERYQTVYAR
CCCCCCCCCEEEECCCCCEEEEEECCCHHHHHHHCCCCCCCHHHCCCCCCHHHHHEEEEE
HPGSVAAPTAGLHFTERLLDELRQRGIRIAPLTLHVGIGTFKPIEVTDVRLHKMHAEWYA
CCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEEEHHHHEEECHHHEE
VPAETVSLIRETRAANRRIVAVGTTAARTLESIADALTGDTEGPITGWTDLYIYPGYHWR
CCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHH
VVDALITNFHLPRSTLILLVASFAGRELILEAYREAVQQRYRFYSFGDAMLIL
HHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA