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The map label for this gene is dnaN [H]

Identifier: 221632726

GI number: 221632726

Start: 708009

End: 709151

Strand: Reverse

Name: dnaN [H]

Synonym: trd_0714

Alternate gene names: 221632726

Gene position: 709151-708009 (Counterclockwise)

Preceding gene: 221632729

Following gene: 221632725

Centisome position: 35.4

GC content: 62.73

Gene sequence:

>1143_bases
GTGCAGCTCAGGTGTCCACAGAGCGAACTCGAGCGAGCTCTGCATCAAGTGTCACGTGCCGTCGCACGGCGCTCGACTCT
GCCAGTCCTGAGTAATGTCCTCCTCGCCGCCGACGAAGCGAACCTCCGCATCACCGCGACGAATCTCGAGATCGCCCTGA
GCGTCAGTTTTCCGGCAGAGGTCACTGATCCCGGCCAACTGAGTGTGCGAGCTGATGTCTTTACCGAATTTATCGGAAGC
CTTCCCAAGCAGGACGTGATCCTCTCCGGCACGACCGGCAGCAACGATCTCCGCGTCACCTGCGGAACCTCCAAGGCGCG
CATCCCTGGTATCCCCGCCGAGGACTTCCCCAGTCTGCCGCGAATCGGTGACCAGCCTCCGACCGCAACTGTGCCGGTCG
AGGGTCTCCGGGAAGCGATCAGCCAGGTCGTGTTCGCAGCGGCGACCGATGACAGTCGGCCAGTCCTCGCCGGAGTCCTG
CTCGAGTTTCGCGGCACGGAGTTGACATTAGCCGCAGCTGATGGTTTCCGCCTGTCGGTACGCTCCCTAGCCCTGCAACA
GCCCGCTGCGACGGACTTGGCCATCATCGTGCCCGCGAGAGCTCTCGGTGAACTGGCTCGCGTCCTCGGCGACGGTGAAG
AAACGGTCCAGCTCCTCGTCACACCGAATCGCAGCCAACTTCTGGCACGCAGTGGCCGACTCGAGTTCCTCTCCCGACTC
ATCGATGGAACCTTTCCCGAGTTCCGGCAAATCATCCCCAAGCAATGGCGTACCCGGGTCACGGTCGACCGCGAATCGTT
CCTCGCGGCAGCCCGCCGAGCCAAGATCTTCGCTCAGTCCAACAACGAGGTAGTCAAGTTGCACATCGCACCGGGTGATT
CCGAACTCGACCCTGGCTCGTTGACTGTTTCCGCCCAGGCAGCCGATGCCGGCGATACCGAGGACATCCTTCCAGCACGG
GTTGACGGTCCGGAGGCGACCATCGCCTTCAACGGCCGCTACTTGACTGACGTCTTGTCCGTCACCAAAGCCACCGAGAT
CGCGCTCGAGATGACGACACCCAACGCTGCGGGCGTCTTTCGACCGGTCGGGGACGAGTCGTTCGTCCACGTCGTGATGC
CGATGGTCATCGGTGCGGCATGA

Upstream 100 bases:

>100_bases
CCGGTAAGCTTCCCGCGTCGGGGTCCCCTCATACTCGAATGCAGGAAGGGCCTGTATAATTCCGGCAGTTGGGTGTACGA
ACAGCGGGGAGGAGCAGTCC

Downstream 100 bases:

>100_bases
CCAGACCGAGGACGGCGACACGATCCAGCCCACCATTGCAGACCCGCCTGTTCTGATACAGGCGAGGATCCCCATGCTGG
TCAGGTCTTTGGAACTCGAA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MQLRCPQSELERALHQVSRAVARRSTLPVLSNVLLAADEANLRITATNLEIALSVSFPAEVTDPGQLSVRADVFTEFIGS
LPKQDVILSGTTGSNDLRVTCGTSKARIPGIPAEDFPSLPRIGDQPPTATVPVEGLREAISQVVFAAATDDSRPVLAGVL
LEFRGTELTLAAADGFRLSVRSLALQQPAATDLAIIVPARALGELARVLGDGEETVQLLVTPNRSQLLARSGRLEFLSRL
IDGTFPEFRQIIPKQWRTRVTVDRESFLAAARRAKIFAQSNNEVVKLHIAPGDSELDPGSLTVSAQAADAGDTEDILPAR
VDGPEATIAFNGRYLTDVLSVTKATEIALEMTTPNAAGVFRPVGDESFVHVVMPMVIGAA

Sequences:

>Translated_380_residues
MQLRCPQSELERALHQVSRAVARRSTLPVLSNVLLAADEANLRITATNLEIALSVSFPAEVTDPGQLSVRADVFTEFIGS
LPKQDVILSGTTGSNDLRVTCGTSKARIPGIPAEDFPSLPRIGDQPPTATVPVEGLREAISQVVFAAATDDSRPVLAGVL
LEFRGTELTLAAADGFRLSVRSLALQQPAATDLAIIVPARALGELARVLGDGEETVQLLVTPNRSQLLARSGRLEFLSRL
IDGTFPEFRQIIPKQWRTRVTVDRESFLAAARRAKIFAQSNNEVVKLHIAPGDSELDPGSLTVSAQAADAGDTEDILPAR
VDGPEATIAFNGRYLTDVLSVTKATEIALEMTTPNAAGVFRPVGDESFVHVVMPMVIGAA
>Mature_380_residues
MQLRCPQSELERALHQVSRAVARRSTLPVLSNVLLAADEANLRITATNLEIALSVSFPAEVTDPGQLSVRADVFTEFIGS
LPKQDVILSGTTGSNDLRVTCGTSKARIPGIPAEDFPSLPRIGDQPPTATVPVEGLREAISQVVFAAATDDSRPVLAGVL
LEFRGTELTLAAADGFRLSVRSLALQQPAATDLAIIVPARALGELARVLGDGEETVQLLVTPNRSQLLARSGRLEFLSRL
IDGTFPEFRQIIPKQWRTRVTVDRESFLAAARRAKIFAQSNNEVVKLHIAPGDSELDPGSLTVSAQAADAGDTEDILPAR
VDGPEATIAFNGRYLTDVLSVTKATEIALEMTTPNAAGVFRPVGDESFVHVVMPMVIGAA

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=376, Percent_Identity=28.9893617021277, Blast_Score=146, Evalue=2e-36,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40542; Mature: 40542

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLRCPQSELERALHQVSRAVARRSTLPVLSNVLLAADEANLRITATNLEIALSVSFPAE
CCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEECCCCEEEEEEEEEEEEEEECCCC
VTDPGQLSVRADVFTEFIGSLPKQDVILSGTTGSNDLRVTCGTSKARIPGIPAEDFPSLP
CCCCCCEEEHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
RIGDQPPTATVPVEGLREAISQVVFAAATDDSRPVLAGVLLEFRGTELTLAAADGFRLSV
CCCCCCCCCCCCHHHHHHHHHHHEEEEECCCCCCHHHHHHHEECCCEEEEEECCCCEEEH
RSLALQQPAATDLAIIVPARALGELARVLGDGEETVQLLVTPNRSQLLARSGRLEFLSRL
HHHHHCCCCCCCEEEEECHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCHHHHHHHH
IDGTFPEFRQIIPKQWRTRVTVDRESFLAAARRAKIFAQSNNEVVKLHIAPGDSELDPGS
HCCCCHHHHHHCCHHHCCEEEECHHHHHHHHHHHEEEEECCCCEEEEEECCCCCCCCCCC
LTVSAQAADAGDTEDILPARVDGPEATIAFNGRYLTDVLSVTKATEIALEMTTPNAAGVF
EEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHEEEEEEEECCCCCCCE
RPVGDESFVHVVMPMVIGAA
ECCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MQLRCPQSELERALHQVSRAVARRSTLPVLSNVLLAADEANLRITATNLEIALSVSFPAE
CCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEECCCCEEEEEEEEEEEEEEECCCC
VTDPGQLSVRADVFTEFIGSLPKQDVILSGTTGSNDLRVTCGTSKARIPGIPAEDFPSLP
CCCCCCEEEHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
RIGDQPPTATVPVEGLREAISQVVFAAATDDSRPVLAGVLLEFRGTELTLAAADGFRLSV
CCCCCCCCCCCCHHHHHHHHHHHEEEEECCCCCCHHHHHHHEECCCEEEEEECCCCEEEH
RSLALQQPAATDLAIIVPARALGELARVLGDGEETVQLLVTPNRSQLLARSGRLEFLSRL
HHHHHCCCCCCCEEEEECHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCHHHHHHHH
IDGTFPEFRQIIPKQWRTRVTVDRESFLAAARRAKIFAQSNNEVVKLHIAPGDSELDPGS
HCCCCHHHHHHCCHHHCCEEEECHHHHHHHHHHHEEEEECCCCEEEEEECCCCCCCCCCC
LTVSAQAADAGDTEDILPARVDGPEATIAFNGRYLTDVLSVTKATEIALEMTTPNAAGVF
EEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHEEEEEEEECCCCCCCE
RPVGDESFVHVVMPMVIGAA
ECCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]