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The map label for this gene is recF

Identifier: 221632725

GI number: 221632725

Start: 706745

End: 707935

Strand: Reverse

Name: recF

Synonym: trd_0713

Alternate gene names: 221632725

Gene position: 707935-706745 (Counterclockwise)

Preceding gene: 221632726

Following gene: 221632722

Centisome position: 35.34

GC content: 61.54

Gene sequence:

>1191_bases
ATGCTGGTCAGGTCTTTGGAACTCGAAGAGTTCCGTTGCTTTCGCCACCTCCATCTCGTCCTCCCGGATCGCGGGCTGCG
CCTCGTCGGCGCGAACGGCTCAGGCAAGACGAGTCTCATCGAGGCGCTCTACATGCTCGCCACGACCAAGTCGTTCCGCG
CCTCGCTGGAGCGTCATCTCGTGCACCGATCGAGCGGGAGCGAACTCGGCATTCCTCCTTATGCTCGCCTCGCTGCCGAA
CTCTTCACGGAGACCGAACGAAGTACGCTGGAAATCGTGCTGATGGTTGACCCCGCCAGCGGCACTGTCAGAAAGCTGTA
TCGCCGGGATGGACGCTCCCTCCGAGCCGTCGAGTTCGTCGGCACACTCCGGGTTGTTCTGTTCTCGCCGGAGGATCTGG
AACTGGTGACGGGAAGCCCGCAGCAGCGTCGACGGTACCTGGATACCATCCTCTCGACGATCGACCGAGCCTATCTGCGC
GCTCTCGCTCGTTACACGCGCATCCTCGAGCACCGGAACAGTCTCTTGAAATCCTTGGCCGAACGGGATCAGCGCGCTGC
TGACGAACAACTCGCGTATTGGGACGAGCAGTTGGTCACGTACGGGGCGTATTTGCTCGTCGCGCGACTTCGTTTTCTCG
CGGAATGGGGACCACGACTCCGCGACCATTTCCAGGCGCTCGATACCCAGGCACAGGTGCTGACAACGGCCTATCTTCCT
TCGATCGACCTTCCGGAAAGTCTCCTCAGCGAACTGGCCGCTCGGGAAGTGGCCGACGCTCAGCTGATCGTGGGAGCACG
GTATCGCGAGACACTGGAACGGCTTCGACCTGACGAGCTGCGGCGGGGAAGCACGCTTGTCGGGCCGCATCGCGACGATG
TGGAGTTCCTGCTGGGCGAGGAGCCACTCACCGCTTTCGGCTCACGCGGCGTGCAGCGCTTGGCCGTGATAGCTGCCAAA
CTAGCCGAAATCGCCGTGATCCACCGCGTCACCGACGACTGGCCTGTCCTGCTTCTCGACGATGCGCTGTCCGAACTCGA
TCAGCAGCATCGAGCGCATCTTTTGGCAACGCTCTCCGCACTACCAGCCCAGTTGATTCTCACAGCTACCGAGTCCGATG
TTCTCGAGACTCCGGTTCTCTCGTCCTTGCCCCTTTTTCGCTTGAATGATGGTCGCCTGGAACCGGGGTGA

Upstream 100 bases:

>100_bases
ATGCCGATGGTCATCGGTGCGGCATGACCAGACCGAGGACGGCGACACGATCCAGCCCACCATTGCAGACCCGCCTGTTC
TGATACAGGCGAGGATCCCC

Downstream 100 bases:

>100_bases
GTCACTGTCCTGCAGCTCACGGACGCTCGACGATCACCGCGATTCCCATACCTCCACCGATACAGAGCGCTGCGGCACCA
TAACGCCCTCCGGTGCGTCG

Product: DNA replication and repair protein recF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MLVRSLELEEFRCFRHLHLVLPDRGLRLVGANGSGKTSLIEALYMLATTKSFRASLERHLVHRSSGSELGIPPYARLAAE
LFTETERSTLEIVLMVDPASGTVRKLYRRDGRSLRAVEFVGTLRVVLFSPEDLELVTGSPQQRRRYLDTILSTIDRAYLR
ALARYTRILEHRNSLLKSLAERDQRAADEQLAYWDEQLVTYGAYLLVARLRFLAEWGPRLRDHFQALDTQAQVLTTAYLP
SIDLPESLLSELAAREVADAQLIVGARYRETLERLRPDELRRGSTLVGPHRDDVEFLLGEEPLTAFGSRGVQRLAVIAAK
LAEIAVIHRVTDDWPVLLLDDALSELDQQHRAHLLATLSALPAQLILTATESDVLETPVLSSLPLFRLNDGRLEPG

Sequences:

>Translated_396_residues
MLVRSLELEEFRCFRHLHLVLPDRGLRLVGANGSGKTSLIEALYMLATTKSFRASLERHLVHRSSGSELGIPPYARLAAE
LFTETERSTLEIVLMVDPASGTVRKLYRRDGRSLRAVEFVGTLRVVLFSPEDLELVTGSPQQRRRYLDTILSTIDRAYLR
ALARYTRILEHRNSLLKSLAERDQRAADEQLAYWDEQLVTYGAYLLVARLRFLAEWGPRLRDHFQALDTQAQVLTTAYLP
SIDLPESLLSELAAREVADAQLIVGARYRETLERLRPDELRRGSTLVGPHRDDVEFLLGEEPLTAFGSRGVQRLAVIAAK
LAEIAVIHRVTDDWPVLLLDDALSELDQQHRAHLLATLSALPAQLILTATESDVLETPVLSSLPLFRLNDGRLEPG
>Mature_396_residues
MLVRSLELEEFRCFRHLHLVLPDRGLRLVGANGSGKTSLIEALYMLATTKSFRASLERHLVHRSSGSELGIPPYARLAAE
LFTETERSTLEIVLMVDPASGTVRKLYRRDGRSLRAVEFVGTLRVVLFSPEDLELVTGSPQQRRRYLDTILSTIDRAYLR
ALARYTRILEHRNSLLKSLAERDQRAADEQLAYWDEQLVTYGAYLLVARLRFLAEWGPRLRDHFQALDTQAQVLTTAYLP
SIDLPESLLSELAAREVADAQLIVGARYRETLERLRPDELRRGSTLVGPHRDDVEFLLGEEPLTAFGSRGVQRLAVIAAK
LAEIAVIHRVTDDWPVLLLDDALSELDQQHRAHLLATLSALPAQLILTATESDVLETPVLSSLPLFRLNDGRLEPG

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=394, Percent_Identity=25.6345177664975, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_THERP (B9KZ04)

Other databases:

- EMBL:   CP001275
- RefSeq:   YP_002521946.1
- GeneID:   7355039
- GenomeReviews:   CP001275_GR
- KEGG:   tro:trd_0713
- OMA:   GQQRTLV
- ProtClustDB:   CLSK974188
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR003593
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- SMART:   SM00382
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 44610; Mature: 44610

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVRSLELEEFRCFRHLHLVLPDRGLRLVGANGSGKTSLIEALYMLATTKSFRASLERHL
CCCCCCCHHHHHHHHHHHHEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VHRSSGSELGIPPYARLAAELFTETERSTLEIVLMVDPASGTVRKLYRRDGRSLRAVEFV
HHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHCCCCCHHHHHH
GTLRVVLFSPEDLELVTGSPQQRRRYLDTILSTIDRAYLRALARYTRILEHRNSLLKSLA
HHEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERDQRAADEQLAYWDEQLVTYGAYLLVARLRFLAEWGPRLRDHFQALDTQAQVLTTAYLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCC
SIDLPESLLSELAAREVADAQLIVGARYRETLERLRPDELRRGSTLVGPHRDDVEFLLGE
CCCCCHHHHHHHHHHHHCCHHHHCCHHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHCCC
EPLTAFGSRGVQRLAVIAAKLAEIAVIHRVTDDWPVLLLDDALSELDQQHRAHLLATLSA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
LPAQLILTATESDVLETPVLSSLPLFRLNDGRLEPG
CCHHHEEEECCCHHHCCCHHHCCCCEEECCCCCCCC
>Mature Secondary Structure
MLVRSLELEEFRCFRHLHLVLPDRGLRLVGANGSGKTSLIEALYMLATTKSFRASLERHL
CCCCCCCHHHHHHHHHHHHEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VHRSSGSELGIPPYARLAAELFTETERSTLEIVLMVDPASGTVRKLYRRDGRSLRAVEFV
HHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHCCCCCHHHHHH
GTLRVVLFSPEDLELVTGSPQQRRRYLDTILSTIDRAYLRALARYTRILEHRNSLLKSLA
HHEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERDQRAADEQLAYWDEQLVTYGAYLLVARLRFLAEWGPRLRDHFQALDTQAQVLTTAYLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCC
SIDLPESLLSELAAREVADAQLIVGARYRETLERLRPDELRRGSTLVGPHRDDVEFLLGE
CCCCCHHHHHHHHHHHHCCHHHHCCHHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHCCC
EPLTAFGSRGVQRLAVIAAKLAEIAVIHRVTDDWPVLLLDDALSELDQQHRAHLLATLSA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
LPAQLILTATESDVLETPVLSSLPLFRLNDGRLEPG
CCHHHEEEECCCHHHCCCHHHCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA