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The map label for this gene is tenA [H]

Identifier: 221632564

GI number: 221632564

Start: 533457

End: 534122

Strand: Reverse

Name: tenA [H]

Synonym: trd_0539

Alternate gene names: 221632564

Gene position: 534122-533457 (Counterclockwise)

Preceding gene: 221632565

Following gene: 221632560

Centisome position: 26.67

GC content: 60.96

Gene sequence:

>666_bases
ATGACCAGCTTCACCCAAGAGCTCTGGCGCTCGATCGATCCGATCTACCAGGCGATCGTCGTTCACCCCTTCCTCGTCGG
CCTGACCGACGGCACCTTACCCGAAGAAGCATTTCGCTTTTACGTCGTGCAGGACGCACTTTACCTGCAGGACTATGCCC
GCTGTCTCGCGCTGGCTGCTGCCAAGGCACCGCGCGAGACGTGGTGCGAGCTCTTTGCCGACCACGCCAAAGTAGCGCTG
GTCGTGGAGCGTGCCTTGCACGAGAGTTTCTTCGCTGCCTGGGGACTTTCTCCCGACAAGATCGCTGGCACGCCGTACGC
GCCGACGAACCTCGCGTACACCTCCTATCTTTTGCGGGTCGCCTACGAGCGTCCGTTCGAGGAGGTCATCGGTGCACTGC
TTCCCTGCTATTGGATCTACTGGGAAGTGGGCAAGCACCTGGAACGATCTGGTTCACCGAACCCACTGTATCAGAAGTGG
ATCGACACTTATGCGTCGGAAGAGTACGCAGCTGTCGTCCAGGCGGTACTGGATGTCGCTGACCAGGTGGTGACCGACCT
CCCCGAGTCGCGCCGGCAACCCATTCGGACACACTTCGTTACGACCGCCCGCTACGAGTGGATGTTCTGGGACGCTGCCT
GGCGCTTGGAGCGCTGGCCAGTGTGA

Upstream 100 bases:

>100_bases
TATTCGGGCTGCCTTTCTGGCCACTGCTCGCTACGAGTACCTCTTCTGGGACATGGTCTATACCGGCTCCAACTGGCCCA
TATAGCGAGAGGAGTGGATG

Downstream 100 bases:

>100_bases
GCCTCTGGCGTTCGCACCCGGCCGAGGACCGAGAACGCGTACCACTGGGCGCAGTGATGCAAAGATCGACCGCAGCAGCA
GGTCCGCAAAGCGCGTGGCT

Product: TENA/THI-4 family

Products: NA

Alternate protein names: Thiaminase II; Transcriptional activator tenA [H]

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MTSFTQELWRSIDPIYQAIVVHPFLVGLTDGTLPEEAFRFYVVQDALYLQDYARCLALAAAKAPRETWCELFADHAKVAL
VVERALHESFFAAWGLSPDKIAGTPYAPTNLAYTSYLLRVAYERPFEEVIGALLPCYWIYWEVGKHLERSGSPNPLYQKW
IDTYASEEYAAVVQAVLDVADQVVTDLPESRRQPIRTHFVTTARYEWMFWDAAWRLERWPV

Sequences:

>Translated_221_residues
MTSFTQELWRSIDPIYQAIVVHPFLVGLTDGTLPEEAFRFYVVQDALYLQDYARCLALAAAKAPRETWCELFADHAKVAL
VVERALHESFFAAWGLSPDKIAGTPYAPTNLAYTSYLLRVAYERPFEEVIGALLPCYWIYWEVGKHLERSGSPNPLYQKW
IDTYASEEYAAVVQAVLDVADQVVTDLPESRRQPIRTHFVTTARYEWMFWDAAWRLERWPV
>Mature_220_residues
TSFTQELWRSIDPIYQAIVVHPFLVGLTDGTLPEEAFRFYVVQDALYLQDYARCLALAAAKAPRETWCELFADHAKVALV
VERALHESFFAAWGLSPDKIAGTPYAPTNLAYTSYLLRVAYERPFEEVIGALLPCYWIYWEVGKHLERSGSPNPLYQKWI
DTYASEEYAAVVQAVLDVADQVVTDLPESRRQPIRTHFVTTARYEWMFWDAAWRLERWPV

Specific function: Stimulates the production of several extracellular degradative enzymes at the transcriptional level, probably by interfering with degS and degU, which are required for this effect. Is not an essential protein, but affects the sporulation frequency [H]

COG id: COG0819

COG function: function code K; Putative transcription activator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiaminase-2 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6325378, Length=215, Percent_Identity=30.2325581395349, Blast_Score=80, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324517, Length=219, Percent_Identity=27.3972602739726, Blast_Score=76, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324997, Length=219, Percent_Identity=25.1141552511416, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016084
- InterPro:   IPR004305 [H]

Pfam domain/function: PF03070 TENA_THI-4 [H]

EC number: =3.5.99.2 [H]

Molecular weight: Translated: 25561; Mature: 25430

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSFTQELWRSIDPIYQAIVVHPFLVGLTDGTLPEEAFRFYVVQDALYLQDYARCLALAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKAPRETWCELFADHAKVALVVERALHESFFAAWGLSPDKIAGTPYAPTNLAYTSYLLRV
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHH
AYERPFEEVIGALLPCYWIYWEVGKHLERSGSPNPLYQKWIDTYASEEYAAVVQAVLDVA
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHH
DQVVTDLPESRRQPIRTHFVTTARYEWMFWDAAWRLERWPV
HHHHHHCCHHHCCCHHHHHHHHHHHEEEEEHHHHHHHCCCC
>Mature Secondary Structure 
TSFTQELWRSIDPIYQAIVVHPFLVGLTDGTLPEEAFRFYVVQDALYLQDYARCLALAA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKAPRETWCELFADHAKVALVVERALHESFFAAWGLSPDKIAGTPYAPTNLAYTSYLLRV
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHH
AYERPFEEVIGALLPCYWIYWEVGKHLERSGSPNPLYQKWIDTYASEEYAAVVQAVLDVA
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHH
DQVVTDLPESRRQPIRTHFVTTARYEWMFWDAAWRLERWPV
HHHHHHCCHHHCCCHHHHHHHHHHHEEEEEHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1898926; 9384377 [H]