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The map label for this gene is phsA [H]

Identifier: 221632495

GI number: 221632495

Start: 465271

End: 467526

Strand: Reverse

Name: phsA [H]

Synonym: trd_0469

Alternate gene names: 221632495

Gene position: 467526-465271 (Counterclockwise)

Preceding gene: 221632496

Following gene: 221632494

Centisome position: 23.34

GC content: 64.58

Gene sequence:

>2256_bases
TTGGAGATGGGCATGCGGCGAAGAGATTTTCTCAAGGTGGCCGCAACCTCAGCGACCGGTGCTGTCTTGTTCGCGGGATG
CGGTCGCATCGGAGACGGTGTGCCAGAAAGCGAGTTCAAGATTGCTCAGCCCGTCTTCAACACCAACGATTGGGTGTATG
GGCGTGACGCGTGGTTCGCGACCGCTGCGCCACCGGACCAGGGGGGATACGGACTGATCGTCCGCGTCTTCGAGGGGCGC
GCCAAGAAGGTCGATGGAAACCCTGATTTCCCTGGCATCCATGGCAAGAGCGATGCCCGAGCGCAGGCGCTCGTCCAGGA
GCTGTACCACCCCGATCGTCTCAGCGGAGCGCGTCGAGCCACTTCGCGCGGGAGCGGCCAATTTCAGACTATTCCGTGGG
ATTCAGCTCTCCAAGAAGTGGCCCAGATCCTCCGCGAGGTGGGTGGCGAGCACGCCGTCCTCATCACCGGTCGGCTGAAC
GCATCGCAAGCTGCGATCGTCGCGGCCTTCCGGAAGGCTACTGGCATTCGCCAAGCCGTTCTGGAACCGGACGAGTTCGT
GGTACTCCGCACCGCTATCCGGCGGCTCTTCGGGGTGAGCACGCTCCCGATTCCTGACCTGGCCAACGCGCGCTTTCTTC
TCAACTTCAGTGCCGACTTCTTGCACGGCTGGATCGCACCCGTCGCACTGGCGCACGCCTATGGCGAGTTCCGGCAAGGA
AAGCCGGGTCAGCGCGGGCTGTACTATCACGTCGGCCCTCGGCTCTCGGGGACAGCCGCGAATGCTGACCGATGGTTCCC
GATCATTCCTGGGTCGGAGGGGCTCGTCGCGCTCGCGATCGCTCAGGTGCTCTCGACCGAGGGGAAAGCCCCGCAAGATC
GCCTCGATCCCTACCTGACGGCGCTCGGGCTGGATGCGACCCGACTGGCGAGTGATTACGCGCCCGAACGAGTCGCCCCA
GCCACGGGTCTTTCGGCGGAACAGATCCGCGAACTGGCGCACGCGCTCGTCGAGCATCAGCCGGCCGTGGTCATCGCTGG
TACGAGTGCTGCAGCAGCCACCAATGGTCTTTTCAACTTGGTGGCGACGTTCGGCCTCAATTGGATACTCGGCTCGGTGA
ACCAGCCTGGCGGCCTGCTCCTCAATCCTGAGCCGCCATTGCCCGACCTGCGTGTACCCGATGACGCCATCCTCTCCCTT
CGCGGCTGGACAGAGCTGTTCGAGCAAATTCGATCGGGCACGATCAGCACCGTCCTCGTCTACCGCGCCAATCCGGTGTA
TGCCCTCCCCAGTGCCAGCGGTGCGCGTGAGGCTCTCCAGGCCGCCAAGCATGTCGTCAGCTTCTCGCCCTTCCTCGACG
AGACCACGACCTTGGCTGATCTGGTTTTGCCGGACCAGGCTTGGCTCGAACAATGGGGGTTGAGCGTTCCCGATCCTGGC
CCCGGCTTCGCAGCGGTCGCCATCCAGCAACCGGTGGTTGCGCCCTTCCTGGATGCCCGCGCCTTCGGCGATGTCCTTCT
CGACCTTCTCCGCCTCCTCGGCCAGGACCAGCCGTGGAGCACGCAGGAGCAAGCGACCAAAGCGCTCGCCCTGCAACTCC
GGTCACTCGGCCGCGGAAGCGTTACGGCGACGAACGACAAAGAGTTCCTCGTGACACTCCAGGCGCGAGGTGGGTGGTGG
GACGAGCAGAGTCGGGCGACCGGCAGTGCACCGTCAACGGCCGCAGTGCAGCCGCGCGAGCCGGCCTTTGCGGGCGAGCA
GAACCAGTTCCCTTATGTCCTTCATCCCTATCCATTGCCGTCGCTCGGCTACGGAGAACTCGCCCACCTGCCCTGGCTGC
AGGGACTCCCGGAACCGATCAGCACGAACGTCTGGCAGACCTGGATCGAAGTGAGTCAGGCGACCGCGGACGAACTCGGG
CTCCAGACCGGTGATCTTGTCCGCGTCCTCACGCCACAGGGAGTCGCTGAGCTACCGGTTTACGTCAACCCAGCAACGCA
GCCTGGAATCATCGCCATTCCGTTCGGGCAGGGACATACAGCGTACACGCGGTACGCCGAGGGACGCGGAGTCAATCCGG
CGACGCTCGTTGCGCCAGAGACCGAACCGGATACCGGTACCTTCGCCTGGGCAGCCACGCGCTGCCGGATCGAGAAGACC
GGACGCCGCGCTCGCCTAGCTCGCTTCGAGGGTATGGTGCCAGCGTTCCAGCTGGAGGAGTTCCCGATCGTCCGCGTGAT
GGCACCGAAGGCCTGA

Upstream 100 bases:

>100_bases
TGCATTACCTGCCACCAGCAGAACGGCGCACGGACTGACTGCTCGGTGTGCCACTACTGATCCGAACAGACGGCTCGACG
ACGATGTGGGGAGGCTGACA

Downstream 100 bases:

>100_bases
GCGGGAGGGATCAGGGAGAGGAAAAGGACGGGACGCATGCCACGCTGGGGAATGGTGATCGATCTTGACCGCTGTATCGG
GTGCGGCGCCTGCGTCGTCG

Product: putative 4Fe-4S ferredoxin, iron-sulfur binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 751; Mature: 751

Protein sequence:

>751_residues
MEMGMRRRDFLKVAATSATGAVLFAGCGRIGDGVPESEFKIAQPVFNTNDWVYGRDAWFATAAPPDQGGYGLIVRVFEGR
AKKVDGNPDFPGIHGKSDARAQALVQELYHPDRLSGARRATSRGSGQFQTIPWDSALQEVAQILREVGGEHAVLITGRLN
ASQAAIVAAFRKATGIRQAVLEPDEFVVLRTAIRRLFGVSTLPIPDLANARFLLNFSADFLHGWIAPVALAHAYGEFRQG
KPGQRGLYYHVGPRLSGTAANADRWFPIIPGSEGLVALAIAQVLSTEGKAPQDRLDPYLTALGLDATRLASDYAPERVAP
ATGLSAEQIRELAHALVEHQPAVVIAGTSAAAATNGLFNLVATFGLNWILGSVNQPGGLLLNPEPPLPDLRVPDDAILSL
RGWTELFEQIRSGTISTVLVYRANPVYALPSASGAREALQAAKHVVSFSPFLDETTTLADLVLPDQAWLEQWGLSVPDPG
PGFAAVAIQQPVVAPFLDARAFGDVLLDLLRLLGQDQPWSTQEQATKALALQLRSLGRGSVTATNDKEFLVTLQARGGWW
DEQSRATGSAPSTAAVQPREPAFAGEQNQFPYVLHPYPLPSLGYGELAHLPWLQGLPEPISTNVWQTWIEVSQATADELG
LQTGDLVRVLTPQGVAELPVYVNPATQPGIIAIPFGQGHTAYTRYAEGRGVNPATLVAPETEPDTGTFAWAATRCRIEKT
GRRARLARFEGMVPAFQLEEFPIVRVMAPKA

Sequences:

>Translated_751_residues
MEMGMRRRDFLKVAATSATGAVLFAGCGRIGDGVPESEFKIAQPVFNTNDWVYGRDAWFATAAPPDQGGYGLIVRVFEGR
AKKVDGNPDFPGIHGKSDARAQALVQELYHPDRLSGARRATSRGSGQFQTIPWDSALQEVAQILREVGGEHAVLITGRLN
ASQAAIVAAFRKATGIRQAVLEPDEFVVLRTAIRRLFGVSTLPIPDLANARFLLNFSADFLHGWIAPVALAHAYGEFRQG
KPGQRGLYYHVGPRLSGTAANADRWFPIIPGSEGLVALAIAQVLSTEGKAPQDRLDPYLTALGLDATRLASDYAPERVAP
ATGLSAEQIRELAHALVEHQPAVVIAGTSAAAATNGLFNLVATFGLNWILGSVNQPGGLLLNPEPPLPDLRVPDDAILSL
RGWTELFEQIRSGTISTVLVYRANPVYALPSASGAREALQAAKHVVSFSPFLDETTTLADLVLPDQAWLEQWGLSVPDPG
PGFAAVAIQQPVVAPFLDARAFGDVLLDLLRLLGQDQPWSTQEQATKALALQLRSLGRGSVTATNDKEFLVTLQARGGWW
DEQSRATGSAPSTAAVQPREPAFAGEQNQFPYVLHPYPLPSLGYGELAHLPWLQGLPEPISTNVWQTWIEVSQATADELG
LQTGDLVRVLTPQGVAELPVYVNPATQPGIIAIPFGQGHTAYTRYAEGRGVNPATLVAPETEPDTGTFAWAATRCRIEKT
GRRARLARFEGMVPAFQLEEFPIVRVMAPKA
>Mature_751_residues
MEMGMRRRDFLKVAATSATGAVLFAGCGRIGDGVPESEFKIAQPVFNTNDWVYGRDAWFATAAPPDQGGYGLIVRVFEGR
AKKVDGNPDFPGIHGKSDARAQALVQELYHPDRLSGARRATSRGSGQFQTIPWDSALQEVAQILREVGGEHAVLITGRLN
ASQAAIVAAFRKATGIRQAVLEPDEFVVLRTAIRRLFGVSTLPIPDLANARFLLNFSADFLHGWIAPVALAHAYGEFRQG
KPGQRGLYYHVGPRLSGTAANADRWFPIIPGSEGLVALAIAQVLSTEGKAPQDRLDPYLTALGLDATRLASDYAPERVAP
ATGLSAEQIRELAHALVEHQPAVVIAGTSAAAATNGLFNLVATFGLNWILGSVNQPGGLLLNPEPPLPDLRVPDDAILSL
RGWTELFEQIRSGTISTVLVYRANPVYALPSASGAREALQAAKHVVSFSPFLDETTTLADLVLPDQAWLEQWGLSVPDPG
PGFAAVAIQQPVVAPFLDARAFGDVLLDLLRLLGQDQPWSTQEQATKALALQLRSLGRGSVTATNDKEFLVTLQARGGWW
DEQSRATGSAPSTAAVQPREPAFAGEQNQFPYVLHPYPLPSLGYGELAHLPWLQGLPEPISTNVWQTWIEVSQATADELG
LQTGDLVRVLTPQGVAELPVYVNPATQPGIIAIPFGQGHTAYTRYAEGRGVNPATLVAPETEPDTGTFAWAATRCRIEKT
GRRARLARFEGMVPAFQLEEFPIVRVMAPKA

Specific function: Oxidoreductase which produces hydrogen sulfide from thiosulfate (Potential) [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=709, Percent_Identity=21.5796897038082, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 80860; Mature: 80860

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMGMRRRDFLKVAATSATGAVLFAGCGRIGDGVPESEFKIAQPVFNTNDWVYGRDAWFA
CCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCCCCCEEEECCCEEE
TAAPPDQGGYGLIVRVFEGRAKKVDGNPDFPGIHGKSDARAQALVQELYHPDRLSGARRA
ECCCCCCCCCEEEEEEECCCCEECCCCCCCCCCCCCCHHHHHHHHHHHCCCHHCCHHHHH
TSRGSGQFQTIPWDSALQEVAQILREVGGEHAVLITGRLNASQAAIVAAFRKATGIRQAV
HCCCCCCEEECCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
LEPDEFVVLRTAIRRLFGVSTLPIPDLANARFLLNFSADFLHGWIAPVALAHAYGEFRQG
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCC
KPGQRGLYYHVGPRLSGTAANADRWFPIIPGSEGLVALAIAQVLSTEGKAPQDRLDPYLT
CCCCCCEEEEECCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHCHHHH
ALGLDATRLASDYAPERVAPATGLSAEQIRELAHALVEHQPAVVIAGTSAAAATNGLFNL
HHCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHH
VATFGLNWILGSVNQPGGLLLNPEPPLPDLRVPDDAILSLRGWTELFEQIRSGTISTVLV
HHHHCHHHEEECCCCCCCEEECCCCCCCCCCCCHHHHEEHHHHHHHHHHHHCCCEEEEEE
YRANPVYALPSASGAREALQAAKHVVSFSPFLDETTTLADLVLPDQAWLEQWGLSVPDPG
EECCCEEECCCCCCHHHHHHHHHHHHHCCCHHCCHHHHHHHHCCCHHHHHHCCCCCCCCC
PGFAAVAIQQPVVAPFLDARAFGDVLLDLLRLLGQDQPWSTQEQATKALALQLRSLGRGS
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCC
VTATNDKEFLVTLQARGGWWDEQSRATGSAPSTAAVQPREPAFAGEQNQFPYVLHPYPLP
EEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCC
SLGYGELAHLPWLQGLPEPISTNVWQTWIEVSQATADELGLQTGDLVRVLTPQGVAELPV
CCCCCHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCE
YVNPATQPGIIAIPFGQGHTAYTRYAEGRGVNPATLVAPETEPDTGTFAWAATRCRIEKT
EECCCCCCCEEEEECCCCCCHHEEECCCCCCCCCEEECCCCCCCCCEEEEEEEEEEHHHC
GRRARLARFEGMVPAFQLEEFPIVRVMAPKA
CCHHHHHHHHCCCCCEECCCCCEEEEECCCC
>Mature Secondary Structure
MEMGMRRRDFLKVAATSATGAVLFAGCGRIGDGVPESEFKIAQPVFNTNDWVYGRDAWFA
CCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCCCCCEEEECCCEEE
TAAPPDQGGYGLIVRVFEGRAKKVDGNPDFPGIHGKSDARAQALVQELYHPDRLSGARRA
ECCCCCCCCCEEEEEEECCCCEECCCCCCCCCCCCCCHHHHHHHHHHHCCCHHCCHHHHH
TSRGSGQFQTIPWDSALQEVAQILREVGGEHAVLITGRLNASQAAIVAAFRKATGIRQAV
HCCCCCCEEECCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
LEPDEFVVLRTAIRRLFGVSTLPIPDLANARFLLNFSADFLHGWIAPVALAHAYGEFRQG
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCC
KPGQRGLYYHVGPRLSGTAANADRWFPIIPGSEGLVALAIAQVLSTEGKAPQDRLDPYLT
CCCCCCEEEEECCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHCHHHH
ALGLDATRLASDYAPERVAPATGLSAEQIRELAHALVEHQPAVVIAGTSAAAATNGLFNL
HHCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHH
VATFGLNWILGSVNQPGGLLLNPEPPLPDLRVPDDAILSLRGWTELFEQIRSGTISTVLV
HHHHCHHHEEECCCCCCCEEECCCCCCCCCCCCHHHHEEHHHHHHHHHHHHCCCEEEEEE
YRANPVYALPSASGAREALQAAKHVVSFSPFLDETTTLADLVLPDQAWLEQWGLSVPDPG
EECCCEEECCCCCCHHHHHHHHHHHHHCCCHHCCHHHHHHHHCCCHHHHHHCCCCCCCCC
PGFAAVAIQQPVVAPFLDARAFGDVLLDLLRLLGQDQPWSTQEQATKALALQLRSLGRGS
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCC
VTATNDKEFLVTLQARGGWWDEQSRATGSAPSTAAVQPREPAFAGEQNQFPYVLHPYPLP
EEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCC
SLGYGELAHLPWLQGLPEPISTNVWQTWIEVSQATADELGLQTGDLVRVLTPQGVAELPV
CCCCCHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCE
YVNPATQPGIIAIPFGQGHTAYTRYAEGRGVNPATLVAPETEPDTGTFAWAATRCRIEKT
EECCCCCCCEEEEECCCCCCHHEEECCCCCCCCCEEECCCCCCCCCEEEEEEEEEEHHHC
GRRARLARFEGMVPAFQLEEFPIVRVMAPKA
CCHHHHHHHHCCCCCEECCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7751291; 11677609; 7737516 [H]